scholarly journals Effect ofWithania SomniferaRoot Powder on the Levels of Circulatory Lipid Peroxidation and Liver Marker Enzymes in Chronic Hyperammonemia

2008 ◽  
Vol 5 (4) ◽  
pp. 872-877 ◽  
Author(s):  
B. Harikrishnan ◽  
P. Subramanian ◽  
S. Subash
Keyword(s):  
Lipid Peroxidation ◽  
Ammonium Chloride ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Underlying Mechanism ◽  
Antioxidant Property ◽  
Marker Enzymes ◽  
Liver Marker Enzymes ◽  
Lipid Peroxidation Products ◽  
Liver Markers

Withania somnifera(L) Dunal (Solanaceae), commonly called Ashwagandha (Sanskrit) is an Ayurvedic Indian medicinal plant, which has been widely used as a home remedy for several ailments. We have investigated the influence ofW.somniferaroot powder on the levels of circulatory ammonia, urea, lipid peroxidation products such as TBARS (thiobarbituric acid and reactive substances), HP (hydroperoxides) and liver marker enzymes such as AST (aspartate transaminase), ALT (alanine transaminase) and ALP (alkaline phosphatase), for its hepatoprotective effect in ammonium chloride induced hyperammonemia. Ammonium chloride treated rats showed a significant increase in the levels of circulatory ammonia, urea, AST, ALT, ALP, TBARS and HP. These changes were significantly decreased in rats treated withW.somniferaroot powder and ammonium chloride. Our results indicate thatW.somniferaoffers hepatoprotection by influencing the levels of lipid peroxidation products and liver markers in experimental hyperammonemia and this could be due to (i) the presence of alkaloids, withanolids and flavonoids, (ii) normalizing the levels of urea and urea related compounds, (iii) its free radical scavenging property and (iv) its antioxidant property. The exact underlying mechanism is still unclear and further research needed.

scholarly journals Influence of Hibiscus sabdariffa (Gongura) on the levels of circulatory lipid peroxidation products and liver marker enzymes in experimental hyperammonemia

2006 ◽  
Vol 4 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Musthafa Mohamed Essa ◽  
Perumal Subramanian ◽  
Ganapathy Suthakar ◽  
Tamilarasan Manivasagam ◽  
Kadiyala Babu Dakshayani ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Hibiscus Sabdariffa ◽  
Marker Enzymes ◽  
Liver Marker Enzymes ◽  
Lipid Peroxidation Products

scholarly journals Antioxidant Potential ofMomordica Charantiain Ammonium Chloride-Induced Hyperammonemic Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
A. Justin Thenmozhi ◽  
P. Subramanian
Keyword(s):  
Body Weight ◽  
Ammonium Chloride ◽  
Thiobarbituric Acid ◽  
Underlying Mechanism ◽  
Antioxidant Potential ◽  
Oxidative Stress Biomarkers ◽  
Plasma Urea ◽  
Male Wistar Rats ◽  
Liver Markers ◽  
And Oxidative Stress

The present study was aimed to investigate the antioxidant potential ofMomordica charantiafruit extract (MCE) in ammonium chloride-induced (AC) hyperammonemic rats. Experimental hyperammonemia was induced in adult male Wistar rats (180–200 g) by intraperitoneal injections of ammonium chloride (100 mg kg−1body weight) thrice a week. The effect of oral administration (thrice a week for 8 consecutive weeks) of MCE (300 mg kg−1body weight) on blood ammonia, plasma urea, serum liver marker enzymes and oxidative stress biomarkers in normal and experimental animals was analyzed. Hyperammonemic rats showed a significant increase in the activities of thiobarbituric acid reactive substances, hydroperoxides and liver markers (alanine transaminase, aspartate transaminase and alkaline phosphatase), and the levels of glutathione peroxidase, superoxide dismutase, catalase and reduced glutathione were decreased in the liver and brain tissues. Treatment with MCE normalized the above-mentioned changes in hyperammonemic rats by reversing the oxidant-antioxidant imbalance during AC-induced hyperammonemia, and offered protection against hyperammonemia. Our results indicate that MCE exerting the antioxidant potentials and maintaining the cellular integrity of the liver tissue could offer protection against AC-induced hyperammonemia. However, the exact underlying mechanism is yet to be investigated, and examination of the efficacy of the active constituents of theM. charantiaon hyperammonemia is desirable.

Antioxidant and Antifungal Properties of Benzimidazole Derivatives

2010 ◽  
Vol 65 (9-10) ◽  
pp. 537-542 ◽  
Author(s):  
Canan Kuş ◽  
Fatma Sözüdönmez ◽  
Benay Can-Eke ◽  
Tülay Çoban
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Radical Scavenging ◽  
Liver Microsomes ◽  
Thiobarbituric Acid ◽  
Benzimidazole Derivatives ◽  
Rat Liver Microsomes ◽  
Inhibitory Effects ◽  
Stable Free Radical ◽  
Radical Scavenging Properties

Antioxidant and radical scavenging properties of a series of 2-[4-(substituted piperazin-/ piperidin-1-ylcarbonyl)phenyl]-1H-benzimidazole derivatives were examined. Free radical scavenging properties of compounds 11-30 and 33 were evaluated for the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide anion radical. In addition the inhibitory effects on the NADPH-dependent lipid peroxidation levels were determined by measuring the formation of 2-thiobarbituric acid reactive substances (TBARS) using rat liver microsomes. Compound 33 which has a p-fluorobenzyl substitutent at position 1 exhibited the strongest inhibition (83%) of lipid peroxidation at a concentration of 10-3 M, while the nonsubstituted analogue 13 caused 57% inhibition. This result is fairly consistent with the antimicrobial activity results against both Staphylococcus aureus and Candida albicans.

scholarly journals Hibiscus sabdariffaAffects Ammonium Chloride-Induced Hyperammonemic Rats

2007 ◽  
Vol 4 (3) ◽  
pp. 321-325 ◽  
Author(s):  
M. Mohamed Essa ◽  
P. Subramanian
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ammonium Chloride ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Alcoholic Extract ◽  
Hibiscus Sabdariffa ◽  
Protein Nitrogen ◽  
Antioxidant Effects ◽  
Brain Tissues

Hibiscus sabdariffa(HS) is an edible medicinal plant, indigenous to India, China and Thailand and is used in Ayurveda and traditional medicine. Alcoholic extract of HS leaves (HSEt) was studied for its anti-hyperammonemic and antioxidant effects in brain tissues of ammonium chloride-induced hyperammonemic rats. Oral administration of HSEt (250 mg kg−1body weight) significantly normalizes the levels of ammonia, urea, uric acid, creatinine and non-protein nitrogen in the blood. HSEt significantly reduced brain levels of lipid peroxidation products such as thiobarbituric acid and reactive substances (TBARS) and hydroperoxides (HP). However, the administered extract significantly increased the levels of antioxidants such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) in brain tissues of hyperammonemic rats. This investigation demonstrates significant anti-hyperammonemic and antioxidant activity of HS.

scholarly journals THERAPEUTIC EFFECT OF NYMPHAEA ALBA LINN. FLOWERS AGAINST ISONIAZID-INDUCED HEPATOTOXICITY: AN EXPERIMENTAL STUDY

2018 ◽  
Vol 11 (5) ◽  
pp. 333
Author(s):  
Mohammad Nasiruddin ◽  
Irfan Ahmad Khan ◽  
Sayeedul Hasan Arif
Keyword(s):  
Biochemical Parameters ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Albino Rats ◽  
Marker Enzymes ◽  
Liver Marker Enzymes ◽  
Stress Markers ◽  
Significant Reversal ◽  
Nymphaea Alba ◽  
Wistar Albino Rats

Objective: This study was designed to evaluate the effect of ethanolic extract of Nymphaea alba (NAEE) Linn. flowers on liver marker enzymes, histology, and antioxidant tests against isoniazid (INH)-induced hepatotoxicity in rats.Methods: Wistar albino rats were treated with INH (50 mg/kg) for 28 days to induce hepatotoxicity. Silymarin (100 mg/kg) and NAEE Linn flowers in 200 mg/kg and 400 mg/kg doses, respectively, were used as standard and test drugs. Liver marker enzymes and histological examination of livers were performed to demonstrate the effect of NAEE against INH-induced hepatotoxicity. Catalase [CAT], glutathione, and malondialdehyde (MDA) levels were estimated to evaluate the antioxidant property of the NAEE extract.Results: The NAEE extract in 200 mg/kg and 400 mg/kg doses significantly decreased INH-induced elevation of liver marker enzymes as well as oxidative stress markers (CAT, glutathione, and MDA) in rats. Changes in biochemical parameters were supported by histological improvements of the liver.Conclusion: The NAEE Linn flowers in 200 mg/kg and 400 mg/kg doses showed a significant reversal of hepatic damage which was induced by INH in rats.

scholarly journals Study of Seminal MDA Level as a Oxidative Stress Marker in Infertile Male

2013 ◽  
Vol 9 (1-2) ◽  
pp. 85-93 ◽  
Author(s):  
Zeba Un-Nahar ◽  
M Ali ◽  
SK Biswas ◽  
N Kamrun ◽  
T Bashar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Male Infertility ◽  
Seminal Fluid ◽  
Sperm Count ◽  
Thiobarbituric Acid ◽  
Underlying Mechanism ◽  
Oxidative Stress Marker ◽  
Infertile Male ◽  
Infertile Group

Male infertility is an important medical and psychosocial problem worldwide. However, the underlying mechanism is far from clear in many cases of male infertility. Apart from known factors, it is therefore very important to identify new and non-conventional factors that may play significant role in male infertility. Oxidative stress has recently been identified as an underlying mechanism of numerous chronic diseases. Some studies have already claimed a relationship between oxidative stress and male infertility. The aim of the present study was therefore to evaluate whether there is an association between oxidative stress and male infertility in Bangladeshi subjects. MDA, the marker of lipid peroxidation was measured in the seminal fluid of fertile and age-matched otherwise healthy infertile male subjects. The sperm count, sperm morphology and motility, as well as semen volume were all found significantly lower in infertile subjects compared with fertile subjects. Interestingly, lipid peroxidation was found elevated seminal fluid of infertile subjects. The MDA was measured by thiobarbituric acid method. In seminal plasma, the median (range) MDA level was found significantly higher in infertile group (3.17 (1.20-6.21) versus 1.88 (0.50-5.37) nmol/ml, p<0.001). DOI: http://dx.doi.org/10.3329/jsf.v9i1-2.14651 J. Sci. Foundation, 9(1&2): 85-93, June-December 2011

scholarly journals Antioxidant Potential of Chestnut (Castanea sativa L.) and Almond (Prunus dulcis L.) By-products

2010 ◽  
Vol 16 (3) ◽  
pp. 209-216 ◽  
Author(s):  
J.C.M. Barreira ◽  
I.C.F.R. Ferreira ◽  
M.B.P.P. Oliveira ◽  
J.A. Pereira
Keyword(s):  
Lipid Peroxidation ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Castanea Sativa ◽  
Thiobarbituric Acid ◽  
Waste Products ◽  
By Products

The antioxidant properties of almond green husks (Cvs. Duro Italiano, Ferraduel, Ferranhês, Ferrastar and Orelha de Mula), chestnut skins and chestnut leaves (Cvs. Aveleira, Boa Ventura, Judia and Longal) were evaluated through several chemical and biochemical assays in order to provide a novel strategy to stimulate the application of waste products as new suppliers of useful bioactive compounds, namely antioxidants. All the assayed by-products revealed good antioxidant properties, with very low EC50 values (lower than 380 μg/mL), particularly for lipid peroxidation inhibition (lower than 140 μg/mL). The total phenols and flavonoids contents were also determined. The correlation between these bioactive compounds and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation in pig brain tissue through formation of thiobarbituric acid reactive substances, was also obtained. Although, all the assayed by-products proved to have a high potential of application in new antioxidants formulations, chestnut skins and leaves demonstrated better results.

Sign in / Sign up

Export Citation Format

Share Document