Streptavidin-Phycoerythrin Conjugated Proteins Bound to Biotin on Langmuir-Blodgett Films of Biotinylated Lipid Monolayers

1990 ◽  
Vol 218 ◽  
Author(s):  
Lynne A. Samuelson ◽  
D. L. Kaplan ◽  
K. A. Marx ◽  
P. Miller ◽  
D. M. Galotti ◽  
...  
Keyword(s):  
Specific Binding ◽  
Lipid Monolayers ◽  
Interface Pressure ◽  
Monolayer Films ◽  
Protein Charge ◽  
Langmuir Blodgett ◽  
Surface Recognition ◽  
Langmuir Blodgett Films ◽  
Pressure Area ◽  
Structure Research

AbstractStudies involving the specific and non-specific surface recognition of biotin on biotinylated LB lipid monolayers by streptavidin and avidin conjugated phycoerythrin are presented. Both streptavidin and avidin conjugates were injected under the monolayer and found to preferentially adsorb to the biotinylated monolayers at the air-water interface. Pressure-area isotherms displayed a biotin-streptavidin/avidin complex dependent increase in surface pressure at expanded areas indicating protein adsorption. The binding of protein was confirmed by transferring the monolayer films to solid supports and measuring the characteristic intense phycoerythrin fluorescence at 576 nm. The effect of protein charge, monolayer packing density and structure, and activation of the tetramer proteins towards specific and non-specific binding are discussed. These results suggest a novel and general methodology for the two-dimensional ordering of protein monolayers with potential bioelectronic, optical and protein structure research applications.

Ordered Langmuir-Blodgett Films of Novel Sandwich-Type Heteroleptic (Porphyrinato) (Phthalocyaninato) Europium Complex

2011 ◽  
Vol 399-401 ◽  
pp. 1097-1101
Author(s):  
Qing Yun Liu ◽  
Qing Ru Zhao ◽  
Hong Guo Liu ◽  
Yong Li
Keyword(s):  
Orientation Angle ◽  
Europium Complex ◽  
Complex Molecules ◽  
Face To Face ◽  
Langmuir Blodgett ◽  
Double Decker ◽  
Vis Spectroscopy ◽  
Langmuir Blodgett Films ◽  
Pressure Area ◽  
Sandwich Type

Ordered molecular assemblies of novel sandwich-type heteroleptic (porphyrinato) (phthalocyaninato) europium complex (a double-decker compound) molecules were fabricated by Langmuir–Blodgett technique. The monolayer behaviors of the complex molecules were investigated using a series of techniques including surface pressure-area isotherm measurement, UV-vis spectroscopy, polarized UV-vis spectroscopy, and low-angle X-ray diffractometry. The results indicated that double-decker complex molecules form well-ordered and stable monolayers with a face-to-face configuration and edge-on arrangement to the water surface at the air-water interface as well as J-aggregates in lamellar multilayers on solid substrates. The orientation angle of the macrocycle in the double-decker compound with respect to the quartz slice surface in the LB films have been determined on the basis of the polarized absorbance and were found to be ca. 42o.

Organometallic Gold(I) Acetylide/Polyoxometalate Hybrid Langmuir-Blodgett Films: Preparation, Characterization, and Photoelectric Properties

2010 ◽  
Vol 63 (1) ◽  
pp. 103 ◽  
Author(s):  
Li Liu ◽  
Qian Liu ◽  
Ming Chen ◽  
Ming-Jian Li ◽  
Li-Ping Xu ◽  
...  
Keyword(s):  
Pure Water ◽  
Monolayer Films ◽  
Microscopy Imaging ◽  
Lb Films ◽  
Quenching Effect ◽  
Force Microscopy ◽  
Langmuir Blodgett ◽  
Photoelectric Properties ◽  
Langmuir Blodgett Films ◽  
Tunnelling Microscopy

A new series of organometallic/inorganic hybrid Langmuir-Blodgett (LB) films containing an organometallic gold(i) aryleneethynylene metal complex (OMA) as the π-conjugated organometallic molecule and a polyoxometalate (POM, POM = H3BW12O40, H6As2W18O62, or H6P2Mo18O62) of the Keggin and Dawson structure as the inorganic composite, were prepared and characterized by π–A isotherms, UV-vis absorption spectra, photoluminescence spectra, atomic force microscopy imaging, scanning tunnelling microscopy, and surface photovoltage spectroscopy. Our experimental results indicate that stable, well defined and well organized Langmuir and LB films are formed in pure water and polyoxometalate subphases. There is a quenching effect in the luminescence of the gold(i) complex when it is made into the LB films. The OMA/POM hybrid LB films display strong photovoltage effect in the UV-vis region. The photovoltage response of OMA/H6As2W18O62 can be as high as 3338 μV. OMA/POM monolayer films show good electrical conductivity, and the tunnelling current for a OMA/H3BW12O40 monolayer film amounts to 50 to approximately –100 nA when the voltage is set at ±9 V.

The monomolecular organization of a photodynamic protein system through specific surface recognition of streptavidin by biotinylated Langmuir-Blodgett films

Langmuir ◽  
1992 ◽  
Vol 8 (2) ◽  
pp. 604-608 ◽  
Author(s):  
Lynne A. Samuelson ◽  
Pascal Miller ◽  
Dianne M. Galotti ◽  
Kenneth A. Marx ◽  
Jayant Kumar ◽  
...  
Keyword(s):  
Specific Surface ◽  
Langmuir Blodgett ◽  
Protein System ◽  
Surface Recognition ◽  
Langmuir Blodgett Films

Nanomechanical properties of ordered phthalocyanine Langmuir–Blodgett layers

2004 ◽  
Vol 19 (5) ◽  
pp. 1461-1470 ◽  
Author(s):  
Tammy Oshiro ◽  
Arnie Backstrom ◽  
Ann-Marie Cumberlidge ◽  
K.W. Hipps ◽  
Ursula Mazur ◽  
...  
Keyword(s):  
Copper Phthalocyanine ◽  
Multilayer Structures ◽  
Monolayer Films ◽  
One Dimensional ◽  
Langmuir Blodgett ◽  
Nanomechanical Properties ◽  
Lb Film ◽  
Pressure Area ◽  
Additional Stability ◽  
Interlayer Structure

The mechanical properties of monolayer and multilayer structures of several symmetrically substituted alkoxy copper phthalocyanine, CuPc(OR)8, Langmuir–Blodgett (LB) films were evaluated. One-dimensional compliance of the monolayers was determined from the slopes of the surface pressure–area isotherms. Multilayers were examined using a SPM and a commercial nanoindentation system. The nanomechanical studies show a distinct relationship between the position and length of alkoxy substituents on the Pc macrocycle and the LB film elasticity and hardness. The phthalocyanine complexes with peripheral octabutoxy and octaoctyloxy substituents form stiff LB monolayers and multilayers. The nonperipheral alkoxy derivetized CuPc films were less stiff. For monolayer films, it appears that the extent of π-π interaction determines the strength of the film. In multilayers, significant additional stability can be imparted through interdigitation of long paraffinic chains which play a significant role in determining the interlayer structure of the LB layers.

Effects of deposition parameters on morphology of Langmuir-blodgett films of poly(γ-Benzyl-L-Glutamate) by scanning-probe-microscopy

1994 ◽  
Vol 52 ◽  
pp. 1058-1059
Author(s):  
D. L. Smith ◽  
I. H. Musselman ◽  
E. P. Enriquez ◽  
V. F. Guarisco ◽  
E. T. Samulski
Keyword(s):  
Liquid Crystalline ◽  
Sample Surface ◽  
Contact Forces ◽  
Structural Motif ◽  
Spectroscopic Techniques ◽  
Monolayer Films ◽  
Langmuir Blodgett ◽  
Deposition Parameters ◽  
Langmuir Blodgett Films ◽  
Soft Samples

Synthetic homopolypeptides, such as poly(γ-benzyl-L-glutamate)(PBLG) bear oc-helical secondary structure and exhibit liquid-crystalline behavior. These properties are useful for a range of materials applications, among them nonlinear optics (NLO). The α-helical structural motif of PBLG can be exploited by regular substitution of chemical functionalities in the sidechains along the main axis. Macromolecular order can then be established in polypeptide thin films using Langmuir-Blodgett (LB) deposition. Monolayer films of PBLG have been investigated by surface spectroscopic techniques, without having an understanding of the local morphology.Atomic force microscopy (AFM) has the potential for elucidating the microstructure of LB films, providing a mechanism for relating differing morphologies to deposition parameters. Excessive contact forces exerted by an AFM tip at small size scales, however, make the technique less viable for delicate organic monolayers. By intermittently striking a sample surface with a sharp probe, the tapping mode AFM provides a means for nondestructively imaging such soft samples.

Langmuir-blodgett films of liquid-crystalline heteroaromatic cations associated with diamagnetic (BF4-, CIO4-) or paramagnetic (TCNQ[math]) anions

1988 ◽  
Vol 85 ◽  
pp. 385-389 ◽  
Author(s):  
M. Vandevyver ◽  
J. Richard ◽  
A Barraud ◽  
M. Veber ◽  
C Jallabert ◽  
...  
Keyword(s):  
Liquid Crystalline ◽  
Langmuir Blodgett ◽  
Langmuir Blodgett Films

Non linear physical properties of langmuir-blodgett films : their possible applications

1988 ◽  
Vol 85 ◽  
pp. 1093-1097 ◽  
Author(s):  
G.G. Roberts ◽  
B. Holcroft ◽  
T. Richardson ◽  
R. Colbrook
Keyword(s):  
Physical Properties ◽  
Langmuir Blodgett ◽  
Non Linear ◽  
Langmuir Blodgett Films
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