The monomolecular organization of a photodynamic protein system through specific surface recognition of streptavidin by biotinylated Langmuir-Blodgett films

Langmuir ◽  
1992 ◽  
Vol 8 (2) ◽  
pp. 604-608 ◽  
Author(s):  
Lynne A. Samuelson ◽  
Pascal Miller ◽  
Dianne M. Galotti ◽  
Kenneth A. Marx ◽  
Jayant Kumar ◽  
...  
Keyword(s):  
Specific Surface ◽  
Langmuir Blodgett ◽  
Protein System ◽  
Surface Recognition ◽  
Langmuir Blodgett Films

Streptavidin-Phycoerythrin Conjugated Proteins Bound to Biotin on Langmuir-Blodgett Films of Biotinylated Lipid Monolayers

1990 ◽  
Vol 218 ◽  
Author(s):  
Lynne A. Samuelson ◽  
D. L. Kaplan ◽  
K. A. Marx ◽  
P. Miller ◽  
D. M. Galotti ◽  
...  
Keyword(s):  
Specific Binding ◽  
Lipid Monolayers ◽  
Interface Pressure ◽  
Monolayer Films ◽  
Protein Charge ◽  
Langmuir Blodgett ◽  
Surface Recognition ◽  
Langmuir Blodgett Films ◽  
Pressure Area ◽  
Structure Research

AbstractStudies involving the specific and non-specific surface recognition of biotin on biotinylated LB lipid monolayers by streptavidin and avidin conjugated phycoerythrin are presented. Both streptavidin and avidin conjugates were injected under the monolayer and found to preferentially adsorb to the biotinylated monolayers at the air-water interface. Pressure-area isotherms displayed a biotin-streptavidin/avidin complex dependent increase in surface pressure at expanded areas indicating protein adsorption. The binding of protein was confirmed by transferring the monolayer films to solid supports and measuring the characteristic intense phycoerythrin fluorescence at 576 nm. The effect of protein charge, monolayer packing density and structure, and activation of the tetramer proteins towards specific and non-specific binding are discussed. These results suggest a novel and general methodology for the two-dimensional ordering of protein monolayers with potential bioelectronic, optical and protein structure research applications.

Langmuir-blodgett films of liquid-crystalline heteroaromatic cations associated with diamagnetic (BF4-, CIO4-) or paramagnetic (TCNQ[math]) anions

1988 ◽  
Vol 85 ◽  
pp. 385-389 ◽  
Author(s):  
M. Vandevyver ◽  
J. Richard ◽  
A Barraud ◽  
M. Veber ◽  
C Jallabert ◽  
...  
Keyword(s):  
Liquid Crystalline ◽  
Langmuir Blodgett ◽  
Langmuir Blodgett Films

Non linear physical properties of langmuir-blodgett films : their possible applications

1988 ◽  
Vol 85 ◽  
pp. 1093-1097 ◽  
Author(s):  
G.G. Roberts ◽  
B. Holcroft ◽  
T. Richardson ◽  
R. Colbrook
Keyword(s):  
Physical Properties ◽  
Langmuir Blodgett ◽  
Non Linear ◽  
Langmuir Blodgett Films

Manufacturing of direct exfoliation graphene-based transparent conducting Langmuir – Blodgett films

2018 ◽  
pp. 17-28
Author(s):  
E. A. Danilov ◽  
◽  
V. M. Samoilov ◽  
V. S. Dmitrieva ◽  
A. V. Nikolaeva ◽  
...  
Keyword(s):  
Langmuir Blodgett ◽  
Transparent Conducting ◽  
Langmuir Blodgett Films

Formation and tribological properties of octacosanoic acid monomolecular Langmuir–Blodgett films

2021 ◽  
pp. 1-7
Author(s):  
Viktoryia Akulova ◽  
Aliaksandr Salamianski ◽  
Ignat Chishankov ◽  
Vladimir Agabekov
Keyword(s):  
Tribological Properties ◽  
Langmuir Blodgett ◽  
Langmuir Blodgett Films

Infrared studies of valinomycin-containing Langmuir-Blodgett films

Langmuir ◽  
1989 ◽  
Vol 5 (2) ◽  
pp. 330-332 ◽  
Author(s):  
V. A. Howarth ◽  
M. C. Petty ◽  
G. H. Davies ◽  
J. Yarwood
Keyword(s):  
Langmuir Blodgett ◽  
Infrared Studies ◽  
Langmuir Blodgett Films
Sign in / Sign up

Export Citation Format

Share Document