Optimizing Process Variables to Control Fiber Diameter of Electrospun Polycaprolactone Nanofiber Using Factorial Design

2011 ◽  
Vol 1316 ◽  
Author(s):  
Saida P. Khan ◽  
Kadambari Bhasin ◽  
Golam M. Newaz
Keyword(s):  
Dielectric Constant ◽  
Factorial Design ◽  
Fiber Diameter ◽  
Polymer Concentration ◽  
Engineering Application ◽  
Electrospinning Process ◽  
Solution Flow Rate ◽  
Tissue Engineering Application ◽  
Electrospinning Technique ◽  
Solution Flow

ABSTRACTIn the electrospinning process, fibers ranging from 50 nm to 1000 nm or greater can be produced by applying an electric potential to a polymeric solution [1, 2]. Our group has studied the fabrication of electro-spun Poly-caprolactone (PCL) nanofiber consisting of a range of fiber diameter (nm-um) and pore sizes. PCL is a biocompatible, FDA approved and biodegradable [3, 4] polymer. As a solvent we have used 2,2,2-trifluoroethanol (TFE) for its biocompatibility, conductivity and high dielectric constant. The electrospinning technique consists of a simple setup with a number of variables working in a complex and unpredictable way. The variables affecting fiber diameter are polymer concentration in the solution, flow rate, applied voltage, tip to collector distance, diameter of the needle/capillary, polymer/solvent dielectric constant etc. In our study we have found that concentration of the solution and molecular weight of the polymer are the most important parameters for forming the nanofibers and viscosity is important for the fiber diameter. To optimize so many variables to control the fiber diameter, we have used the factorial design method. The study is important for the fabrication of biomimetic scaffold for vascular implant and tissue engineering application.

scholarly journals Bioactivity Assessment of Poly(ɛ-caprolactone)/Hydroxyapatite Electrospun Fibers for Bone Tissue Engineering Application

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Mohd Izzat Hassan ◽  
Naznin Sultana ◽  
Salehhuddin Hamdan
Keyword(s):  
Body Fluid ◽  
Geometrical Structure ◽  
Fiber Diameter ◽  
Engineering Application ◽  
High Water ◽  
Electrospinning Process ◽  
Tissue Engineering Application ◽  
Nanofibrous Structure ◽  
Homogenous Distribution ◽  
Adjustment Of Parameters

Electrospinning is useful for fabricating nanofibrous structure with different composition and morphologies. It offers great advantages through its geometrical structure and biomimetic property, which can provide a suitable environmental site for cell growth. The fiber diameter is entangled by the concentration of PCL with some adjustment of parameters during electrospinning process. PCL with lower concentration had bead structure while higher concentration had smooth fiber. The incorporation of nanoparticle hydroxyapatite (nHA) into poly(ɛ-caprolactone) fiber was studied. The fiber diameter of PCL was increased with the addition of nHA. Composition of fiber at lower concentrations of PCL and nHA into the polymer produced fiber with a homogenous distribution of nHA in PCL fiber with less agglomeration. The immersion of PCL/nHA fiber in simulated body fluid (SBF) had bone-like apatite layer on its surface while PCL showed no results. PCL/nHA showed high water uptake and had improved wettability compared to PCL alone, suggesting that PCL/nHA fibers were more hydrophilic than PCL fiber.

scholarly journals Towards Analysis and Optimization of Electrospun PVP (Polyvinylpyrrolidone) Nanofibers

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Utkarsh ◽  
Hussien Hegab ◽  
Muhammad Tariq ◽  
Nabeel Ahmed Syed ◽  
Ghaus Rizvi ◽  
...  
Keyword(s):  
Sound Absorption ◽  
Fiber Diameter ◽  
Polymer Concentration ◽  
Electrospinning Process ◽  
Average Model ◽  
Model Accuracy ◽  
Electrospinning Technique ◽  
Array Design ◽  
Polymeric Nanofibers ◽  
Experimental Trials

In this study, the polymeric nanofibers of polyvinylpyrrolidone (PVP) were manufactured using the electrospinning technique. The electrospinning process parameters such as voltage, polymer concentration, rotational speed of the collecting drum, collecting distance, and flow rate were optimized to obtain the minimum fiber diameter for sound absorption applications. The effects of these parameters on the fiber diameter as output responses were investigated by analysis of variance (ANOVA) and Taguchi’s array design. Furthermore, a mathematical model was generated using response surface methodology (RSM) to model the electrospinning process. The high voltage and polymer concentration were observed to be the most significant parameters at 95% and 99% confidence level. The average model accuracy of 83.4% was observed for the predictive model of electrospinning which is considered acceptable as it is composed of complete experimental trials of 27 out of 243 runs. The experimental study offers a promising attempt in the open literature to carefully understand the effect of various electrospinning parameters when producing PVP nanofibers.

scholarly journals Electrospun Nylon-6 Nanofibers and Their Characteristics

2020 ◽  
Vol 9 (1) ◽  
pp. 9-19
Author(s):  
Ida Sriyanti ◽  
Meily P Agustini ◽  
Jaidan Jauhari ◽  
Sukemi Sukemi ◽  
Zainuddin Nawawi
Keyword(s):  
Fiber Diameter ◽  
Peak Shift ◽  
Nylon 6 ◽  
Electrospinning Process ◽  
Electrospinning Technique ◽  
Fiber Concentration ◽  
Air Filters ◽  
Xrd Pattern ◽  
Electrospinning Method ◽  
Small Wave

The purposes of this research were to investigate the synthesized Nylon-6 nanofibers using electrospinning technique and their characteristics. The method used in this study was an experimental method with a quantitative approach. Nylon-6 nanofibers have been produced using the electrospinning method. This fiber was made with different concentrations, i.e. 20% w/w (FN1), 25% w/w (FN2), and 30% w/w (FN3). The SEM results show that the morphology of all nylon-6 nanofibers) forms perfect fibers without bead fiber. Increasing fiber concentration from 20% w/w to 30% w/w results in bigger morphology and fiber diameter. The dimensions of the FN1, FN2, and FN3 fibers are 1890 nm, 2350 nm, and 2420 nm, respectively. The results of FTIR analysis showed that the increase in the concentration of nylon-6 (b) and the electrospinning process caused a peak shift in the amide II group (CH2 bond), the carbonyl group and the CH2 stretching of the amide III group from small wave numbers to larger ones. The results of XRD characterization showed that the electrospinning process affected the changes in the XRD pattern of nylon-6 nanofiber (FN1, FN2, and FN3) in the state of semi crystal. Nylon-6 nanofibers can be used for applications in medicine, air filters, and electrode for capacitors

Effects of Process Parameters on Formation of Hybrid Tissue Constructs

2013 ◽  
Author(s):  
Karen Chang Yan ◽  
Pamela Hitscherich ◽  
James Ferrie
Keyword(s):  
Tissue Engineering ◽  
Processing Parameters ◽  
Living Cells ◽  
Live Cells ◽  
Solution Flow Rate ◽  
Electrospinning Technique ◽  
Solution Flow ◽  
Tissue Constructs ◽  
Key Issues ◽  
Working Distance

Tissue engineering is a promising aspect of regenerative medicine that is aimed at constructing functional tissues and organs. While progresses in tissue engineering have led successful clinic applications, challenges remain for more complex tissues/organs that require concerted efforts from multiple types of cells. One of the key issues in building replacements for complex tissues/organs is to mimic the organ’s complex natural organization using a mixture of engineered materials and living cells [1]. Electrospinning has shown promise as a technique to create the microenvironment necessary for cell growth and proliferation for tissue engineering applications[2–4], while multiple fabrication methods have been developed to manipulate live cells(e.g. cell printing) [5–7]. To this end, a system integrating polymer electrospinning technique and pressure-driven cell deposition method is currently under development for forming hybrid tissue constructs with living cells and polymers. This study focuses on examining morphology of electrospun fibers as function of processing parameters including working distance and solution flow rate.

scholarly journals Designing Multifunctional Protective PVC Electrospun Fibers with Tunable Properties

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2086
Author(s):  
Pedro J. Rivero ◽  
Iker Rosagaray ◽  
Juan P. Fuertes ◽  
José F. Palacio ◽  
Rafael J. Rodríguez
Keyword(s):  
Fiber Diameter ◽  
Electrospinning Process ◽  
Operating Parameters ◽  
Electrospun Fibers ◽  
Polymeric Precursor ◽  
Operational Parameters ◽  
Electrospinning Technique ◽  
Coating Morphology ◽  
Hydrophobic Nature ◽  
First Time

In this work, the electrospinning technique is used for the fabrication of electrospun functional fibers with desired properties in order to show a superhydrophobic behavior. With the aim to obtain a coating with the best properties, a design of experiments (DoE) has been performed by controlling several inputs operating parameters, such as applied voltage, flow rate, and precursor polymeric concentration. In this work, the reference substrate to be coated is the aluminum alloy (60661T6), whereas the polymeric precursor is the polyvinyl chloride (PVC) which presents an intrinsic hydrophobic nature. Finally, in order to evaluate the coating morphology for the better performance, the following parameters—such as fiber diameter, surface roughness (Ra, Rq), optical properties, corrosion behavior, and wettability—have been deeply analyzed. To sum up, this is the first time that DoE has been used for the optimization of superhydrophobic or anticorrosive surfaces by using PVC precursor for the prediction of an adequate surface morphology as a function of the input operational parameters derived from electrospinning process with the aim to validate better performance.

scholarly journals Morphology andIn VitroBehavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Toshihiro Inami ◽  
Yasuhiro Tanimoto ◽  
Masayuki Ueda ◽  
Yo Shibata ◽  
Satoshi Hirayama ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Biomedical Applications ◽  
Polymer Concentration ◽  
The Body ◽  
Solution Flow Rate ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
Solution Flow

This work describes the fabrication, optimization, and characterization of electrospun fibrous poly(D,L-lactic acid) (PDLLA) for biomedical applications. The influences of the polymer concentration of the electrospinning solution (5, 10, or 15 wt%) and the solution flow rate (0.1, 0.5, 1.0, or 2.0 mL/h) on the morphology of the obtained fibrous PDLLA were evaluated. Thein vitrobiocompatibility of two types of PDLLA, ester terminated PDLLA (PDLLA-R) and carboxyl terminated PDLLA (PDLLA-COOH), was evaluated by monitoring apatite formation on samples immersed in Hanks’ balanced salt (HBS) solution. 15 wt% polymer solution was the most beneficial for preparing a fibrous PDLLA structure. Meanwhile, no differences in morphology were observed for PDLLA prepared at various flow rates. Apatite precipitate is formed on both types of PDLLA only 1 day after immersion in HBS solution. After 7 days of immersion, PDLLA-COOH showed greater apatite formation ability compared with that of PDLLA-R, as measured by thin-film X-ray diffraction. The results indicated that the carboxyl group is effective for apatite precipitation in the body environment.

Physicochemical and biological investigation of oxygen plasma modified electrospun polyurethane scaffolds for connective tissue engineering application

2019 ◽  
Vol 39 (6) ◽  
pp. 526-533
Author(s):  
Farnaz Ghorbani ◽  
Ali Zamanian
Keyword(s):  
Tissue Engineering ◽  
Plasma Treatment ◽  
Oxygen Plasma ◽  
Fiber Diameter ◽  
Engineering Application ◽  
Biological Behavior ◽  
Plasma Modification ◽  
Diameter Distribution ◽  
Oxygen Plasma Treatment ◽  
Tissue Engineering Application

AbstractIn this study, electrospinning was selected to fabricate randomly oriented polyurethane (PU) nanofibers for tissue engineering application, and the surface of scaffolds was exposed to oxygen plasma flow. The morphology structure of the PU scaffolds before and after oxygen plasma treatment was observed using scanning electron microscopy (SEM) micrographs, and the fiber diameter distribution was measured using Image J software. The results demonstrated that oxygen plasma modification reduces the fiber diameter without any other special effects on fiber microstructure. Water drop contact angle and swelling ratio of PU constructs were performed to estimate the water-scaffolds interactions. The results revealed improvement of hydrophilicity by oxygen plasma treatment. Atomic force microscopy test was done to analyze a topological characteristic of the scaffolds, and it was found out that oxygen plasma treatment decreases the roughness of the scaffolds. The biological behavior of the scaffolds was investigated by SEM observation and MTT assay after L-929 fibroblast cells culture.In vitroassays demonstrated biocompatibility, cellular attachments, and filopodia formation on plasma modified samples. These results suggest that oxygen plasma treatment improves the physicochemical and biological properties of PU scaffolds to create a more hydrophilic surface which facilitates cell attachments and proliferation.

scholarly journals Comparative Study of One-Step Cross-Linked Electrospun Chitosan-Based Membranes

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Yanet E. Aguirre-Chagala ◽  
Laura B. Pavón-Pérez ◽  
Víctor Altuzar ◽  
Jorge G. Domínguez-Chávez ◽  
Severino Muñoz-Aguirre ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Engineering Application ◽  
Electrospinning Process ◽  
Light Change ◽  
Tissue Engineering Application ◽  
Major Drawback ◽  
Close Relationship ◽  
Uva Light ◽  
One Step ◽  
Physical Resistance

Chitosan membranes are widely applied for tissue engineering; however, a major drawback is their low resistance in aqueous phases and therefore the structure collapses impeding their long-term use. Although there is extensive research, because of chitosan’s importance as a biomaterial, studies involving chitosan-based membranes are still needed. Herein, a detailed investigation of diverse chemical routes to cross-link fibers in situ by electrospinning process is described. In case of using genipin as cross-linker, a close relationship with the content and the mean diameter values is reported, suggesting a crucial effect over the design of nanostructures. Also, the physical resistance is enhanced for the combination of two types of methods, such as chemical and physical methods. Cross-linked fibers upon exposure to long wave ultraviolet A (UVA light) change their morphology, but not their chemical composition. When they are incubated in aqueous phase for 70 days, they show an extensive improvement of their macrostructural integrity which makes them attractive candidates for tissue engineering application. As a result, the thermal properties of these materials reveal less crystallinity and higher temperature of degradation.

The Effect of Processing Parameters Onpoly (Lactic Acid)/Poly(Ethylene Oxide)Bicomponentultrafine Fibersby Co-Electrospinning

2013 ◽  
Vol 701 ◽  
pp. 254-258 ◽  
Author(s):  
Suttipan Pavasupree ◽  
Kawee Srikulkit ◽  
Ratthapol Rangkupan
Keyword(s):  
Lactic Acid ◽  
Flow Rate ◽  
Applied Voltage ◽  
Water Soluble ◽  
Flow Rate Ratio ◽  
Poly Lactic Acid ◽  
Solution Flow Rate ◽  
Electrospinning Technique ◽  
Solution Flow ◽  
Water Soluble Polymer

Poly (lactic acid) (PLA)/polyethylene oxide (PEO) bicomponent fibers werefabricated by co-electrospinning technique in a side by side configuration. Effect of PEO concentration, PLA and PEO solution flow rate and an applied voltage on formation, size and morphology of the fibers were investigated. The results showed that the fibers size increased with increasing PEO concentration, PEO flow rate ratio and applied voltage. The composition of the fibers was confirmed by IR spectrum. Additionally, by pairing PEO, which is a water soluble polymer, with PLA, follow by PEO phase removal in water, a C-shaped ultrafine fiber was prepared.

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