scholarly journals Bioactivity Assessment of Poly(ɛ-caprolactone)/Hydroxyapatite Electrospun Fibers for Bone Tissue Engineering Application

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Mohd Izzat Hassan ◽  
Naznin Sultana ◽  
Salehhuddin Hamdan
Keyword(s):  
Body Fluid ◽  
Geometrical Structure ◽  
Fiber Diameter ◽  
Engineering Application ◽  
High Water ◽  
Electrospinning Process ◽  
Tissue Engineering Application ◽  
Nanofibrous Structure ◽  
Homogenous Distribution ◽  
Adjustment Of Parameters

Electrospinning is useful for fabricating nanofibrous structure with different composition and morphologies. It offers great advantages through its geometrical structure and biomimetic property, which can provide a suitable environmental site for cell growth. The fiber diameter is entangled by the concentration of PCL with some adjustment of parameters during electrospinning process. PCL with lower concentration had bead structure while higher concentration had smooth fiber. The incorporation of nanoparticle hydroxyapatite (nHA) into poly(ɛ-caprolactone) fiber was studied. The fiber diameter of PCL was increased with the addition of nHA. Composition of fiber at lower concentrations of PCL and nHA into the polymer produced fiber with a homogenous distribution of nHA in PCL fiber with less agglomeration. The immersion of PCL/nHA fiber in simulated body fluid (SBF) had bone-like apatite layer on its surface while PCL showed no results. PCL/nHA showed high water uptake and had improved wettability compared to PCL alone, suggesting that PCL/nHA fibers were more hydrophilic than PCL fiber.

Optimizing Process Variables to Control Fiber Diameter of Electrospun Polycaprolactone Nanofiber Using Factorial Design

2011 ◽  
Vol 1316 ◽  
Author(s):  
Saida P. Khan ◽  
Kadambari Bhasin ◽  
Golam M. Newaz
Keyword(s):  
Dielectric Constant ◽  
Factorial Design ◽  
Fiber Diameter ◽  
Polymer Concentration ◽  
Engineering Application ◽  
Electrospinning Process ◽  
Solution Flow Rate ◽  
Tissue Engineering Application ◽  
Electrospinning Technique ◽  
Solution Flow

ABSTRACTIn the electrospinning process, fibers ranging from 50 nm to 1000 nm or greater can be produced by applying an electric potential to a polymeric solution [1, 2]. Our group has studied the fabrication of electro-spun Poly-caprolactone (PCL) nanofiber consisting of a range of fiber diameter (nm-um) and pore sizes. PCL is a biocompatible, FDA approved and biodegradable [3, 4] polymer. As a solvent we have used 2,2,2-trifluoroethanol (TFE) for its biocompatibility, conductivity and high dielectric constant. The electrospinning technique consists of a simple setup with a number of variables working in a complex and unpredictable way. The variables affecting fiber diameter are polymer concentration in the solution, flow rate, applied voltage, tip to collector distance, diameter of the needle/capillary, polymer/solvent dielectric constant etc. In our study we have found that concentration of the solution and molecular weight of the polymer are the most important parameters for forming the nanofibers and viscosity is important for the fiber diameter. To optimize so many variables to control the fiber diameter, we have used the factorial design method. The study is important for the fabrication of biomimetic scaffold for vascular implant and tissue engineering application.

Physicochemical and biological investigation of oxygen plasma modified electrospun polyurethane scaffolds for connective tissue engineering application

2019 ◽  
Vol 39 (6) ◽  
pp. 526-533
Author(s):  
Farnaz Ghorbani ◽  
Ali Zamanian
Keyword(s):  
Tissue Engineering ◽  
Plasma Treatment ◽  
Oxygen Plasma ◽  
Fiber Diameter ◽  
Engineering Application ◽  
Biological Behavior ◽  
Plasma Modification ◽  
Diameter Distribution ◽  
Oxygen Plasma Treatment ◽  
Tissue Engineering Application

AbstractIn this study, electrospinning was selected to fabricate randomly oriented polyurethane (PU) nanofibers for tissue engineering application, and the surface of scaffolds was exposed to oxygen plasma flow. The morphology structure of the PU scaffolds before and after oxygen plasma treatment was observed using scanning electron microscopy (SEM) micrographs, and the fiber diameter distribution was measured using Image J software. The results demonstrated that oxygen plasma modification reduces the fiber diameter without any other special effects on fiber microstructure. Water drop contact angle and swelling ratio of PU constructs were performed to estimate the water-scaffolds interactions. The results revealed improvement of hydrophilicity by oxygen plasma treatment. Atomic force microscopy test was done to analyze a topological characteristic of the scaffolds, and it was found out that oxygen plasma treatment decreases the roughness of the scaffolds. The biological behavior of the scaffolds was investigated by SEM observation and MTT assay after L-929 fibroblast cells culture.In vitroassays demonstrated biocompatibility, cellular attachments, and filopodia formation on plasma modified samples. These results suggest that oxygen plasma treatment improves the physicochemical and biological properties of PU scaffolds to create a more hydrophilic surface which facilitates cell attachments and proliferation.

scholarly journals Comparative Study of One-Step Cross-Linked Electrospun Chitosan-Based Membranes

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Yanet E. Aguirre-Chagala ◽  
Laura B. Pavón-Pérez ◽  
Víctor Altuzar ◽  
Jorge G. Domínguez-Chávez ◽  
Severino Muñoz-Aguirre ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Engineering Application ◽  
Electrospinning Process ◽  
Light Change ◽  
Tissue Engineering Application ◽  
Major Drawback ◽  
Close Relationship ◽  
Uva Light ◽  
One Step ◽  
Physical Resistance

Chitosan membranes are widely applied for tissue engineering; however, a major drawback is their low resistance in aqueous phases and therefore the structure collapses impeding their long-term use. Although there is extensive research, because of chitosan’s importance as a biomaterial, studies involving chitosan-based membranes are still needed. Herein, a detailed investigation of diverse chemical routes to cross-link fibers in situ by electrospinning process is described. In case of using genipin as cross-linker, a close relationship with the content and the mean diameter values is reported, suggesting a crucial effect over the design of nanostructures. Also, the physical resistance is enhanced for the combination of two types of methods, such as chemical and physical methods. Cross-linked fibers upon exposure to long wave ultraviolet A (UVA light) change their morphology, but not their chemical composition. When they are incubated in aqueous phase for 70 days, they show an extensive improvement of their macrostructural integrity which makes them attractive candidates for tissue engineering application. As a result, the thermal properties of these materials reveal less crystallinity and higher temperature of degradation.

Processing of polycaprolactone and hydroxyapatite to fabricate graded electrospun composites for tendon-bone interface regeneration

2017 ◽  
Vol 37 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Ece Bayrak ◽  
Burak Ozcan ◽  
Cevat Erisken
Keyword(s):  
Concentration Distribution ◽  
Fiber Diameter ◽  
Engineering Application ◽  
Model Systems ◽  
Diameter Distribution ◽  
Tissue Engineering Application ◽  
Mineral Concentration ◽  
Bone Interface ◽  
Fiber Diameter Distribution ◽  
Mesh Fiber

Abstract The process of electrospinning is utilized with different approaches including conventional electrospinning, extrusion electrospinning, and electroblowing to form nanofibrous meshes and composites. Here, we report on the quality and properties of spatially graded polycaprolactone (PCL) and nano-hydroxyapatite (nHA) composite meshes fabricated with multiple-spinneret electrospinning. The composite meshes were characterized in terms of the amount of spatially allocated nHA concentration across the mesh, fiber diameter, porosity, pore size, and hydrophilicity of meshes. Results show that linearly and continuously varying nHA concentration distribution, i.e. graded structure, can be accomplished across the mesh thickness using multiple-spinneret electrospinning, which is in accordance with the change of mineral concentration observed in native tendon-bone interface. Furthermore, incorporation of nanoparticles into nanofibers led to increased fiber diameter as depicted by a shift in fiber diameter distribution, a significant increase in mean fiber diameter from 361±9 nm to 459±21 nm, and an increase in contact angle from 120.01±2.77° to 115.24±1.17°. These findings suggest that the composite meshes formed in this study could serve as model systems to be used as scaffolds in tendon-bone tissue engineering application in particular, and for other tissue-tissue interfaces in a broader context.

scholarly journals Effects of Chitosan Alkali Pretreatment on the Preparation of Electrospun PCL/Chitosan Blend Nanofibrous Scaffolds for Tissue Engineering Application

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Fatemeh Roozbahani ◽  
Naznin Sultana ◽  
Ahmad Fauzi Ismail ◽  
Hamed Nouparvar
Keyword(s):  
Tissue Engineering ◽  
Molecular Weight ◽  
Treatment Time ◽  
Engineering Application ◽  
Electrospinning Process ◽  
Wound Dressings ◽  
Tissue Engineering Application ◽  
Sem Images ◽  
Nanofibrous Scaffolds ◽  
3D Network

Recently, nanofibrous scaffolds have been used in the field of biomedical engineering as wound dressings, tissue engineering scaffolds, and drug delivery applications. The electrospun nanofibrous scaffolds can be used as carriers for several types of drugs, genes, and growth factors. PCL is one of the most commonly applied synthetic polymers for medical use because of its biocompatibility and slow biodegradability. PCL is hydrophobic and has no cell recognition sites on its structure. Electrospinning of chitosan and PCL blend was investigated in formic acid/acetic acid as the solvent with different PCL/chitosan ratios. High viscosity of chitosan solutions makes difficulties in the electrospinning process. Strong hydrogen bonds in a 3D network in acidic condition prevent the movement of polymeric chains exposed to the electrical field. Consequently, the amount of chitosan in PCL/chitosan blend was limited and more challenging when the concentration of PCL increases. The treatment of chitosan in alkali condition under high temperature reduced its molecular weight. Longer treatment time further decreased the molecular weight of chitosan and hence its viscosity. Electrospinning of PCL/chitosan blend was possible at higher chitosan ratio, and SEM images showed a decrease in fiber diameter and narrower distribution with increase in the chitosan ratio.

Coating of Laponite on PLA Nanofibrous for Bone Tissue Engineering Application

2021 ◽  
Vol 29 (3) ◽  
pp. 191-198
Author(s):  
Zahra Orafa ◽  
Shiva Irani ◽  
Ali Zamanian ◽  
Hadi Bakhshi ◽  
Habib Nikukar ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Bone Tissue Engineering Application

Electrospun nano-bio membrane for bone tissue engineering application- a new approach

2020 ◽  
Vol 249 ◽  
pp. 123010 ◽  
Author(s):  
Senthil Rethinam ◽  
Bahri Basaran ◽  
Sumathi Vijayan ◽  
Ali Mert ◽  
Oğuz Bayraktar ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
New Approach ◽  
Bone Tissue Engineering Application

Preparation of Fibrous Scaffolds Containing Calcium and Silicon Species

2011 ◽  
Vol 493-494 ◽  
pp. 840-843
Author(s):  
Akiko Obata ◽  
Hiroki Ozasa ◽  
Julian R. Jones ◽  
Toshihiro Kasuga
Keyword(s):  
Tissue Engineering ◽  
Hybrid Materials ◽  
Fiber Diameter ◽  
Fiber Spinning ◽  
High Porosity ◽  
Cotton Wool ◽  
Tissue Engineering Scaffolds ◽  
Large Defect ◽  
Nanofibrous Structure ◽  
Versatile Technique

Materials for bone defect filling should have 3D macroporous structure and be flexible to be packed into complex defects with limited entrance space. Tissue engineering scaffolds should also mimic the structure and morphology of the host tissue. Electrospinning is a versatile technique to produce materials with micro/nanofibrous structure, large surface area and high porosity. Electrospun materials are very promising for tissue engineering due to the possibility of mimicking the fibrous structure of natural extra cellular matrix (ECM). Siloxane-containing vaterite (SiV)/poly (L-lactic acid) (PLLA) hybrids (SiPVH) with controlled silicate and calcium ions releasing ability has been produced in our group. They have also demonstrated good cell infiltration into the electrospun hybrid materials that had fiber diameters greater than 10 μm. However, these electrospun hybrid materials were planar (2D) and are not suitable for large defect regeneration. In this work, the development of a fabrication technique for the production of 3D cotton wool-like structures with fiber diameter in the range of 10 μm was performed. SiPVH cotton wool-like structure containing 0, 30 and 60 wt % SiV were prepared by blowing air in the direction perpendicular to fiber spinning. Si-vaterite particles and small pores were found on the surface of the fibers. The fiber diameter of the samples were found to be in the range of 10 ~ 20 μm. Stretch tests showed more than 50 % extension for the SiPVH cotton wool-like material containing 30 wt % SiV (SiPVH30). This extension was similar to that observed for the PLLA cotton wool-like material. The results suggest that the SiPVH30 cotton wool-like material are good candidates for bone tissue engineering scaffolds.

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