Investigation of Normal Cell and Cancer Cell Attachment and the Effects of Ganoderma Lucidum Using an Electric Impedance Sensing Technique

MRS Advances ◽  
2020 ◽  
Vol 5 (45) ◽  
pp. 2341-2348
Author(s):  
Steffi Shi Qing Kong ◽  
Alejandra Martinez ◽  
Maddy Behravan
Keyword(s):  
Ganoderma Lucidum ◽  
Cell Attachment ◽  
A431 Cell ◽  
Hacat Cells ◽  
Electric Impedance ◽  
A431 Cells ◽  
Impedance Sensing ◽  
Impedance Data ◽  
Before And After ◽  
Cellular Matrix

AbstractThis research introduces an application of an electric impedance sensing technique to investigate cell attachment of normal epithelial cells (HaCAT) and cancerous cells (A431) before and after addition of Ganoderma Lucidum (reishi). In this study, an impedance sensing system is used to measure and characterize real-time changes in electric impedance (resistance and capacitance) with respect to an alternating current (AC) applied to HaCAT and A431 cell colonies. The impedance data is related to the properties of cell spreading, attachment, and delamination. The effect of reishi at dosages of 0.005, 0.01, and 0.02 mg/ml on these cellular properties was inferred from impedance data. The initial impedance data show that resistance is greater for A431 cells than HaCAT cells and that capacitance for A431 cells is less than the capacitance for HaCAT cells. Further, the data shows the resistance for HaCAT cells and for A431 cells increases with time, and the capacitance for both decreases with time. The impedance data analysis shows that reishi plays no role in altering the impedance of the cellular matrix. At most, reishi serves as a lubricant to allow partial detachment and reattachment of HaCAT cell-to-cell bonds, thus reordering (< entropy) the cellular matrix. This effect is not seen in A431 cell colonies.

scholarly journals Comparison of Leading Biosensor Technologies to Detect Changes in Human Endothelial Barrier Properties in Response to Pro-Inflammatory TNFα and IL1β in Real-Time

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 159
Author(s):  
James J. W. Hucklesby ◽  
Akshata Anchan ◽  
Simon J. O'Carroll ◽  
Charles P. Unsworth ◽  
E. Scott Graham ◽  
...  
Keyword(s):  
Human Brain ◽  
Real Time ◽  
Barrier Properties ◽  
Endothelial Barrier ◽  
Endothelial Monolayer ◽  
Impedance Sensing ◽  
Endothelial Monolayers ◽  
Impedance Data ◽  
Cell Substrate ◽  
Limited Frequency

Electric Cell-Substrate Impedance Sensing (ECIS), xCELLigence and cellZscope are commercially available instruments that measure the impedance of cellular monolayers. Despite widespread use of these systems individually, direct comparisons between these platforms have not been published. To compare these instruments, the responses of human brain endothelial monolayers to TNFα and IL1β were measured on all three platforms simultaneously. All instruments detected transient changes in impedance in response to the cytokines, although the response magnitude varied, with ECIS being the most sensitive. ECIS and cellZscope were also able to attribute responses to particular endothelial barrier components by modelling the multifrequency impedance data acquired by these instruments; in contrast the limited frequency xCELLigence data cannot be modelled. Consistent with its superior impedance sensing, ECIS exhibited a greater capacity than cellZscope to distinguish between subtle changes in modelled endothelial monolayer properties. The reduced resolving ability of the cellZscope platform may be due to its electrode configuration, which is necessary to allow access to the basolateral compartment, an important advantage of this instrument. Collectively, this work demonstrates that instruments must be carefully selected to ensure they are appropriate for the experimental questions being asked when assessing endothelial barrier properties.

In vitro hyperthermia studied in a continuous manner using electric impedance sensing

RSC Advances ◽  
2015 ◽  
Vol 5 (76) ◽  
pp. 62007-62016 ◽  
Author(s):  
Xinwu Xie ◽  
Ran Liu ◽  
Youchun Xu ◽  
Lei Wang ◽  
Ziyang Lan ◽  
...  
Keyword(s):  
Cell Interactions ◽  
Electric Impedance ◽  
Impedance Sensing

A platform based on the ECIS technique was constructed for analyzing heat-cell interactions and further in vitro hyperthermia studies.

Collagen XXII binds to collagen-binding integrins via the novel motifs GLQGER and GFKGER

2014 ◽  
Vol 459 (1) ◽  
pp. 217-227 ◽  
Author(s):  
Daniela Zwolanek ◽  
Guido Veit ◽  
Johannes A. Eble ◽  
Donald Gullberg ◽  
Florence Ruggiero ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Hacat Cells ◽  
The Novel ◽  
Collagen Binding ◽  
Binding Motifs ◽  
Helical Domain ◽  
Triple Helical Domain

Cell attachment to collagens is mediated by integrins. In the present study, we define two new integrin-binding motifs, GLQGER and GFKGER, within the collagen XXII triple helical domain. Mutation of the two motifs in collagen XXII abolishes the binding to HaCaT cells completely.

Comparative study of the human keratinocytes proteome of the HaCaT line: identification of proteins encoded by genes of 18 chromosomes under the influence of detergents

2020 ◽  
Vol 66 (6) ◽  
pp. 469-476
Author(s):  
Y.S. Kisrieva ◽  
N.F. Samenkova ◽  
O.B. Larina ◽  
V.G. Zgoda ◽  
I.I. Karuzina ◽  
...  
Keyword(s):  
Sodium Dodecyl ◽  
Human Keratinocytes ◽  
Tandem Mass ◽  
Hacat Cells ◽  
Hacat Keratinocytes ◽  
Chromosome 18 ◽  
Line Identification ◽  
Before And After ◽  
Hacat Keratinocyte ◽  
Triton X

Using electrospray ionization tandem mass spectrometry, a comparative analysis of the HaCaT keratinocyte proteins encoded by the 18th chromosome was performed before and after exposure to sodium dodecyl sulfate (25 mg/ml) and to Triton X-100 (12.5 mg/ml) in a subtoxic dose for 48 hours. Proteins were identified using the SearchGUI platform (X!Tandem and MS-GF+ search engines). In total, 1284 proteins were found in immortalized human HaCaT keratinocytes and about 75% of them were identified by two or more peptides. Were identified, that 26 proteins were encoded by genes of chromosome 18. Among these proteins, 17 were common for control cells and HaCaT cells treated with SDS. Proteins MARE2 and CTIF were identified only in control keratinocytes. Seven identified proteins encoded by genes of chromosome 18 were found only in detergent-treated keratinocytes: LMAN1, NDUV2, SPB3, VPS4B, KDSR, ROCK1 and RHG28.

SEM Observation of Composite Ceramic Scaffolds’ Surface during Incubation in Culture Medium with or without Human PDL Fibroblasts

2011 ◽  
Vol 493-494 ◽  
pp. 866-871
Author(s):  
Anna Theocharidou ◽  
K. Tsoptsias ◽  
Eleana Kontonasaki ◽  
Lambrini Papadopoulou ◽  
C. Panayiotou ◽  
...  
Keyword(s):  
Culture Medium ◽  
Cell Attachment ◽  
Surface Characteristics ◽  
Composite Ceramic ◽  
Surface Porosity ◽  
Periodontal Ligament Fibroblasts ◽  
Eds Analysis ◽  
Before And After ◽  
And Function

Chitin is a polysaccharide abundant in nature. Its’ deacetylation product-chitosan- in combination with gelatin (collagen product) is commonly used asbiopolymer scaffold for tissue engineering. The aim of this study was to investigate diffrerences in surface characteristics of chitin (CHN CCS) and chitosan –gelatin (CHS-G CCS) composite ceramic scaffolds (CCS), during their incubation in culture medium (DMEM) with or without human periodontal ligament fibroblasts (HPDLF). CHN CCS and CHS- G CCS, with pore size 70-200μm, were fabricated on the surface of ceramic disks, being coated with a mixture of bioactive glass – ceramic (1:1 wt). Three CCSs of each type were constructed. Each CCS was incubated at 37 °C up to 10 days, either only in DMEM supplemented with 10% FCS or in DMEM with the presence of 105HPDLF. SEM microphotographs and EDS analysis, before and after incubation, were used to investigate CCSs’ surface alterations. Before incubation, all type of CCSs appeared to be macro porous with high interconnectivity. Exposed to incubation, CHN CCSs’ surface porosity seemed to be rapidly reduced and a rough surface without pores was observed with or without HPDLF. Attached HPDLF were rarely detected. CHS-G CCSs appeared to retain surface porosity in DMEM without cells. In HPDLF culture an almost uniform surface with organic aggregates and attached cells was observed. Until day 10, HPDLF could only be detected at CHS-G CCS’s surface. Conclusion: SEM microphotographs observations indicate that CHN CCSs’ incubation in DMEM led in early and rapid coalescence of surface pores, thus inhibiting HPDLF attachment. HPDLF attachment on CHS-G CCSs confirm the beneficial role of gelatin, while differences in CHS-G CCSs’ surface with and without HPDLF culture indicate that not only sedimentation of medium's ingredients, but cell attachment and function could decrease surface’s porosity, affecting consequently HPDLF proliferation.

High Sensitivity MEMS Biosensor for Monitoring Cell Attachment

2012 ◽  
Author(s):  
Fei Liu ◽  
Fang Li ◽  
David C. Spray ◽  
Anis Nurashikin Nordin ◽  
Ioana Voiculescu
Keyword(s):  
Resonant Frequency ◽  
Cell Attachment ◽  
High Sensitivity ◽  
Live Cells ◽  
Seeding Density ◽  
Impedance Sensing ◽  
Aortic Endothelial Cells ◽  
Frequency Measurements ◽  
Cell Substrate ◽  
Time Required

This paper presents the fabrication and testing of a novel microelectromechanical (MEMS) biosensor based on live cells. The biosensor combines two biosensing techniques; resonant frequency measurements and electric cell-substrate impedance sensing (ECIS) on a single device. The sensor is based on the innovative placement of the working microelectrode for ECIS technique as the upper electrode of a quartz crystal microbalance (QCM) resonator. This hybrid biosensor was tested with bovine aortic endothelial cells with different seeding densities. The cell attachment and spreading was monitored with both sensors; the QCM and the ECIS technique. After the cells form a monolayer the values of the impedance and resonant frequency measurements are constant. The optimal cell seeding density with minimal time required to attach and form a monolayer was observed to be 1.5×104 cells/cm2. This biosensor monitors the cells attachment and viability and could be used for screening toxicants in drinking water.

Changes in chemical compositions of substrates before and after Ganoderma lucidum cultivation

2010 ◽  
Vol 27 (3) ◽  
pp. 637-642 ◽  
Author(s):  
A. Peksen ◽  
G. Yakupoglu ◽  
T. Yakupoglu ◽  
C. Gulser ◽  
E. Ozturk ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Chemical Compositions ◽  
Before And After

scholarly journals Enhanced Osseointegration and Bio-Decontamination of Nanostructured Titanium Based on Non-Thermal Atmospheric Pressure Plasma

2020 ◽  
Vol 21 (10) ◽  
pp. 3533 ◽  
Author(s):  
Yuhao Zeng ◽  
Satoshi Komasa ◽  
Hisataka Nishida ◽  
Akinori Agariguchi ◽  
Tohru Sekino ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Cell Attachment ◽  
Atmospheric Pressure Plasma ◽  
Plasma Generator ◽  
Matrix Mineralization ◽  
Bone Response ◽  
Plasma Device ◽  
Before And After ◽  
Surface Nanostructure

Alkali-treated titanate layer with nanonetwork structures (TNS) is a promising surface for improving osseointegration capacity in implants. Nevertheless, there is a risk of device failure as a result of insufficient resistance to biofilm contamination. This study tested whether treatment using a handheld non-thermal plasma device could efficiently eliminate biofilm contamination without destroying the surface nanostructure while re-establishing a surface that promoted new bone generation. TNS specimens were treated by a piezoelectric direct discharge (PDD) plasma generator. The effect of decontamination was performed utilizing Staphylococcus aureus. The evaluation of initial cell attachment with adhesion images, alkaline phosphatase activity, extracellular matrix mineralization, and expression of genes related to osteogenesis was performed using rat bone marrow mesenchymal stem cells, and the bone response were evaluated in vivo using a rat femur model. Nanotopography and surface roughness did not significantly differ before and after plasma treatments. Cell and bone formation activity were improved by TNS plasma treatment. Furthermore, plasma treatment effectively eliminated biofilm contamination from the surface. These results suggested that this plasma treatment may be a promising approach for the treatment of nanomaterials immediately before implantation and a therapeutic strategy for peri-implantitis.

scholarly journals Gold Nanoparticles Synthesized with a Polyphenols-Rich Extract from Cornelian Cherry (Cornus mas) Fruits: Effects on Human Skin Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Maria Perde-Schrepler ◽  
Luminita David ◽  
Liliana Olenic ◽  
Monica Potara ◽  
Eva Fischer-Fodor ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Types ◽  
Dna Lesions ◽  
Hydrodynamic Diameter ◽  
Hacat Cells ◽  
A431 Cells ◽  
Cornus Mas ◽  
Normal Keratinocytes ◽  
Biologic Effects ◽  
The Difference

Gold nanoparticles (GNPs) were obtained by green synthesis with an extract fromCornus masfruits (GNPs-CM), characterized by several methods, and their biologic effects were evaluated on two cell lines: HaCaT, normal keratinocytes, and A431, epidermoid carcinoma. GNPs were spherical with sizes between 2 and 24 nm. Their optical spectra had a dominant plasmonic band centered at 525 nm; zeta potential distribution was narrow, centered at −19.7 mV, and the mean hydrodynamic diameter was 58 nm. GNPs were visualized in both cell types entering the cells by endocytosis. The amount of gold uptaken by the cells was dose and time dependent. The intracellular concentration of Au ions was higher in HaCaT compared to A431 cells. The toxicity of GNPs-CM was dose dependent being significant only when the highest concentrations were employed. A431 cells were less affected compared to HaCaT cells, but the difference was not statistically significant. ROS production was not significant, except in HaCaT cells at the highest concentration. The comet assay revealed no significant supplementary DNA lesions, while the secretion of inflammatory cytokines was modulated by the presence of GNPs only when the cells were additionally irradiated with UVB. These results recommend GNPs-CM for further testing and possible dermatological applications.

Apigenin mediated gold nanoparticle synthesis and their anti-cancer effect on human epidermoid carcinoma (A431) cells

RSC Advances ◽  
2015 ◽  
Vol 5 (63) ◽  
pp. 51055-51066 ◽  
Author(s):  
Indra Rajendran ◽  
Harini Dhandapani ◽  
Rajaram Anantanarayanan ◽  
Rama Rajaram
Keyword(s):  
Gold Nanoparticle ◽  
Nanoparticle Synthesis ◽  
Epidermoid Carcinoma ◽  
Hacat Cells ◽  
A431 Cells ◽  
Anti Cancer ◽  
Gold Nanoparticle Synthesis ◽  
Cancer Activity ◽  
Human Epidermoid Carcinoma

Apigenin reduces Au3+to Au0to form ap-AuNPs at RT. ap-AuNPs are biocompatible towards HaCat cells. They show anti-cancer activity towards A431 cells by inducing apoptosis.

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