In vitro hyperthermia studied in a continuous manner using electric impedance sensing

RSC Advances ◽  
2015 ◽  
Vol 5 (76) ◽  
pp. 62007-62016 ◽  
Author(s):  
Xinwu Xie ◽  
Ran Liu ◽  
Youchun Xu ◽  
Lei Wang ◽  
Ziyang Lan ◽  
...  
Keyword(s):  
Cell Interactions ◽  
Electric Impedance ◽  
Impedance Sensing

A platform based on the ECIS technique was constructed for analyzing heat-cell interactions and further in vitro hyperthermia studies.

scholarly journals IGFBP-1 expression is reduced in human type 2 diabetic glomeruli and modulates β1-integrin/FAK signalling in human podocytes

Diabetologia ◽  
2021 ◽  
Author(s):  
Abigail C. Lay ◽  
Lorna J. Hale ◽  
Holly Stowell-Connolly ◽  
Robert J. P. Pope ◽  
Viji Nair ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Igf Binding Proteins ◽  
Impedance Sensing ◽  
Human Type ◽  
Early Stages ◽  
Igf Binding ◽  
Phosphoinositide 3 Kinase ◽  
End Stage

AbstractAims/hypothesisPodocyte loss or injury is one of the earliest features observed in the pathogenesis of diabetic kidney disease (DKD), which is the leading cause of end-stage renal failure worldwide. Dysfunction in the IGF axis, including in IGF binding proteins (IGFBPs), is associated with DKD, particularly in the early stages of disease progression. The aim of this study was to investigate the potential roles of IGFBPs in the development of type 2 DKD, focusing on podocytes.MethodsIGFBPexpression was analysed in the Pima DKD cohort, alongside data from the Nephroseq database, and in ex vivo human glomeruli. Conditionally immortalised human podocytes and glomerular endothelial cells were studied in vitro, where IGFBP-1 expression was analysed using quantitative PCR and ELISAs. Cell responses to IGFBPs were investigated using migration, cell survival and adhesion assays; electrical cell-substrate impedance sensing; western blotting; and high-content automated imaging.ResultsData from the Pima DKD cohort and from the Nephroseq database demonstrated a significant reduction in glomerularIGFBP-1in the early stages of human type 2 DKD. In the glomerulus, IGFBP-1 was predominantly expressed in podocytes and controlled by phosphoinositide 3-kinase (PI3K)–forkhead box O1 (FoxO1) activity. In vitro,IGFBP-1 signalled to podocytes via β1-integrins, resulting in increased phosphorylation of focal-adhesion kinase (FAK), increasing podocyte motility, adhesion, electrical resistance across the adhesive cell layer and cell viability.Conclusions/interpretationThis work identifies a novel role for IGFBP-1 in the regulation of podocyte function and that the glomerular expression ofIGFBP-1is reduced in the early stages of type 2 DKD, via reduced FoxO1 activity. Thus, we hypothesise that strategies to maintain glomerular IGFBP-1 levels may be beneficial in maintaining podocyte function early in DKD.Graphical abstract

Innervated human corneal equivalents as in vitro models for nerve‐target cell interactions

2003 ◽  
Vol 18 (1) ◽  
pp. 170-172 ◽  
Author(s):  
Erik J. Suuronen ◽  
Masatsugu Nakamura ◽  
Mitchell A. Watsky ◽  
Peter K. Stys ◽  
Linda J. Müller ◽  
...  
Keyword(s):  
Target Cell ◽  
In Vitro Models ◽  
Cell Interactions

An Optimized In Vitro Model of the Respiratory Tract Wall to Study Particle Cell Interactions

2006 ◽  
Vol 19 (3) ◽  
pp. 392-405 ◽  
Author(s):  
Fabian Blank ◽  
Barbara M. Rothen-Rutishauser ◽  
Samuel Schurch ◽  
Peter Gehr
Keyword(s):  
Respiratory Tract ◽  
In Vitro Model ◽  
Cell Interactions ◽  
Vitro Model

Cytokine regulation of cell-to-cell interactions in lymphokine-activated killer cell cytotoxicity in vitro

1993 ◽  
Vol 36 (2) ◽  
pp. 76-82 ◽  
Author(s):  
Toshiyuki Takahashi ◽  
Hiroshi Ishikura ◽  
Kazuhiro Iwai ◽  
Chisa Takahashi ◽  
Hiroyuki Kato ◽  
...  
Keyword(s):  
Killer Cell ◽  
Cell Interactions ◽  
Cell Cytotoxicity ◽  
Cytokine Regulation ◽  
Lymphokine Activated Killer Cell

scholarly journals SyNPL: Synthetic Notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo

2021 ◽  
Author(s):  
Mattias Malaguti ◽  
Rosa Portero Migueles ◽  
Jennifer Annoh ◽  
Daina Sadurska ◽  
Guillaume Blin ◽  
...  
Keyword(s):  
Cell Fate ◽  
Cell Lines ◽  
Modular Design ◽  
Cell Interactions ◽  
Cell Populations ◽  
Cell Contact ◽  
Pluripotent Cells ◽  
Cell Fate Decisions ◽  
Cell Cell

ABSTRACTCell-cell interactions govern differentiation and cell competition in pluripotent cells during early development, but the investigation of such processes is hindered by a lack of efficient analysis tools. Here we introduce SyNPL: clonal pluripotent stem cell lines which employ optimised Synthetic Notch (SynNotch) technology to report cell-cell interactions between engineered “sender” and “receiver” cells in cultured pluripotent cells and chimaeric mouse embryos. A modular design makes it straightforward to adapt the system for programming differentiation decisions non-cell-autonomously in receiver cells in response to direct contact with sender cells. We demonstrate the utility of this system by enforcing neuronal differentiation at the boundary between two cell populations. In summary, we provide a new tool which could be used to identify cell interactions and to profile changes in gene or protein expression that result from direct cell-cell contact with defined cell populations in culture and in early embryos, and which can be adapted to generate synthetic patterning of cell fate decisions.

scholarly journals Reconstitution of immune cell interactions in free-standing membranes

2018 ◽  
Author(s):  
Edward Jenkins ◽  
Ana Mafalda Santos ◽  
James H. Felce ◽  
Deborah Hatherley ◽  
Michael L. Dustin ◽  
...  
Keyword(s):  
Synapse Formation ◽  
Immune Cell ◽  
Cell Interactions ◽  
Free Standing ◽  
Immune Synapse ◽  
Signalling Molecules ◽  
Transient Nature ◽  
Spatiotemporal Regulation ◽  
Summary Statement

AbstractThe spatiotemporal regulation of signalling proteins at the contacts formed between immune cells and their targets determines how and when immune responses begin and end. It is important, therefore, to be able to elucidate molecular processes occurring at these interfaces. However, the detailed investigation of each component’s contribution to the formation and regulation of the contact is hampered by the complexity of cellular composition and architecture. Moreover, the transient nature of these interactions creates additional challenges, especially for using advanced imaging technology. One approach to circumventing these problems is to establish in vitro systems that faithfully mimic immune cell interactions, incorporating complexity that can be ‘dialled-in’ as needed. Here, we present an in vitro system making use of synthetic vesicles that mimic important aspects of immune cell surfaces. Using this system, we begin to investigate the spatial distribution of signalling molecules (receptors, kinases and phosphatases) and the intracellular rearrangements that accompany the initiation of signalling in T cells. The model system presented here is expected to be widely applicable.Summary StatementImmune cell-cell interactions are reconstituted in free-standing vesicles wherein spatiotemporal aspects of immune synapse formation can be investigated.

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