The Influence of Cellular Debris on Cell Guidance and Implications for Incorporating Silicon Based Micropatterns

MRS Advances ◽  
2017 ◽  
Vol 2 (57) ◽  
pp. 3537-3546 ◽  
Author(s):  
Delphine Dean ◽  
Katherine Hafner ◽  
Xue Chen ◽  
Brian Kirkland ◽  
Theresa Hafner ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Cell Alignment ◽  
Osteoblast Cells ◽  
Piranha Solution ◽  
Microelectronic Fabrication ◽  
Cellular Debris ◽  
Cell Guidance ◽  
Silicon Based ◽  
Microelectronic Processing ◽  
External Stimuli

ABSTRACTDetermining what external stimuli influence cell differentiation, morphology, and growth continues to be a focus on many research groups to meet the healthcare Grand Challenges. While prior work has shown the influence of stiffness, surface chemistry and topography, these parameters often change in tandem, making it difficult to delineate the role of an individual component. This study examined the possible incorporation of microelectronic processing to produce reusable substrates for cell guidance studies. Subsequent plating of substrates cleaned with methods common in a microelectronic fabrication process showed complex responses including migration. Optical characterization of surfaces after cleaning showed remaining cellular debris that could be removed through the incorporation of a piranha solution. The micro patterned substrates did allow controlled comparison between dental pulp stem cells and osteoblast cells. The dental pulp cells did not show any cell alignment or cell proliferation (as indicated by cell density) with the isotropic or anisotropic micropatterns on the initial plating. The osteoblast cells (control) only aligned with the lines and not any of the other patterns (dots, holes or hexagons).

Odontoblastic Exosomes Attenuate Apoptosis in Neighboring Cells

2019 ◽  
Vol 98 (11) ◽  
pp. 1271-1278 ◽  
Author(s):  
H.S. Wang ◽  
F.H. Yang ◽  
Y.J. Wang ◽  
F. Pei ◽  
Z. Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Dental Pulp ◽  
Ex Vivo ◽  
Intercellular Signaling ◽  
High Concentration ◽  
Rescue Effect ◽  
Apical Papilla ◽  
External Stimuli

Each odontoblast is tightly linked to other odontoblasts. They form a line of defense and are capable of withstanding external stimuli, particularly the inflammation caused by caries. Thus, we investigated exosomes derived from odontoblasts as an intercellular mechanism by which inflamed odontoblasts are protected from apoptosis. CD63, an exosome marker, was expressed at high levels in caries-affected regions of the dental pulp. We conducted an ex vivo experiment by applying different concentrations of lipopolysaccharide (LPS) to the odontoblast-like cells (mineralization was induced in stem cells derived from the apical papilla). Odontoblast-like cells treated with a high concentration of LPS (20 µg/mL LPS, severely affected) exhibited an accelerated release of exosomes, which attenuated the LPS-induced cell apoptosis of odontoblast-like cells treated with a low concentration of LPS (1 µg/mL LPS, mildly affected). Next, we blocked exosome uptake with chlorpromazine, and the rescue effect vanished. Based on our findings, severely inflamed odontoblasts attenuate the apoptosis of mildly inflamed neighboring cells through an exosome-mediated intercellular signaling pathway.

scholarly journals Local Regeneration of Dentin-Pulp Complex Using Controlled Release of FGF-2 and Naturally Derived Sponge-Like Scaffolds

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Chiaki Kitamura ◽  
Tatsuji Nishihara ◽  
Masamichi Terashita ◽  
Yasuhiko Tabata ◽  
Ayako Washio
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Controlled Release ◽  
Dental Pulp ◽  
Traumatic Injury ◽  
Pathological Changes ◽  
Irreversible Damage ◽  
Exogenous Growth ◽  
External Stimuli ◽  
Exogenous Growth Factors

Restorative and endodontic procedures have been recently developed in an attempt to preserve the vitality of dental pulp after exposure to external stimuli, such as caries infection or traumatic injury. When damage to dental pulp is reversible, pulp wound healing can proceed, whereas irreversible damage induces pathological changes in dental pulp, eventually requiring its removal. Nonvital teeth lose their defensive abilities and become severely damaged, resulting in extraction. Development of regeneration therapy for the dentin-pulp complex is important to overcome limitations with presently available therapies. Three strategies to regenerate the dentin-pulp complex have been proposed; regeneration of the entire tooth, local regeneration of the dentin-pulp complex from amputated dental pulp, and regeneration of dental pulp from apical dental pulp or periapical tissues. In this paper, we focus on the local regeneration of the dentin-pulp complex by application of exogenous growth factors and scaffolds to amputated dental pulp.

scholarly journals The Macrophage Iron Signature in Health and Disease

2021 ◽  
Vol 22 (16) ◽  
pp. 8457
Author(s):  
Christina Mertens ◽  
Oriana Marques ◽  
Natalie K. Horvat ◽  
Manuela Simonetti ◽  
Martina U. Muckenthaler ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Blood Cells ◽  
The Body ◽  
Tissue Homeostasis ◽  
Gene Profile ◽  
Physiological Conditions ◽  
Cellular Debris ◽  
Health And Disease ◽  
Fast Adaptation ◽  
External Stimuli

Throughout life, macrophages are located in every tissue of the body, where their main roles are to phagocytose cellular debris and recycle aging red blood cells. In the tissue niche, they promote homeostasis through trophic, regulatory, and repair functions by responding to internal and external stimuli. This in turn polarizes macrophages into a broad spectrum of functional activation states, also reflected in their iron-regulated gene profile. The fast adaptation to the environment in which they are located helps to maintain tissue homeostasis under physiological conditions.

scholarly journals Investigation of Pulse Electric Field Effect on HT29 Cell Alignment Properties Cultured on Laminin Micro-Patterned Surface

2018 ◽  
Vol 7 (3.36) ◽  
pp. 108
Author(s):  
Hassan Buhari Mamman ◽  
Muhammad Mahadi Abdul Jamil ◽  
Mohammed Ahmed Bawa ◽  
Mohamad Nazib Adon
Keyword(s):  
Wound Healing ◽  
Cell Adhesion ◽  
Cell Membrane ◽  
Electric Field ◽  
Pulse Electric Field ◽  
Ht29 Cell ◽  
Cell Physiology ◽  
Cell Alignment ◽  
Cell Guidance ◽  
Pulse Electric

Pulse electric field (PEF) is a way of generating transient holes in the cell membrane. This is achieved by exposing the cell to a high voltage electric field of usually of short duration. The application of PEF to the cell cannot only open pores in the cell membrane but can also affect the cell physiology. Extracellular matrix protein is the major regulator of many cellular functions such as proliferation, adhesion and migration. PEF was also found to modulate these cellular behaviours. However, a combined influence of PEF and ECM on cellular behaviour which could further enhances the cellular processes for wound healing application via directed cell migration has not been investigated. Therefore, the aim of this study is to examine the effect of PEF in combination with ECM on the cell guidance and self-assemble monolayer of HT29 cell line. Cell alignment was investigated via micro-contact printing techniques. The results of the study have shown that PEF has improved the HT29 cell alignment and elongation by more than 40%. Since tissue development in multicellular organisms in the course of wound healing depends on the cell adhesion process which can be influence by electrostatic charges. Therefore, manipulation of substrate charge by patterning the substrate and application of PEF to enhance cell adhesion is a promising scheme that can regulate cell guidance for wound healing application.   

Deposition of ternary silicon based compounds by CVD techniques

1999 ◽  
Vol 09 (PR8) ◽  
pp. Pr8-101-Pr8-107
Author(s):  
F. J. Martí ◽  
A. Castro ◽  
J. Olivares ◽  
C. Gómez-Aleixandre ◽  
J. M. Albella
Keyword(s):  
Silicon Based

CVD mullite and mullite-alumina corrosion protection coatings for silicon based ceramics

2001 ◽  
Vol 11 (PR3) ◽  
pp. Pr3-861-Pr3-867 ◽  
Author(s):  
S. M. Zemskova ◽  
J. A. Haynes ◽  
K. M. Cooley
Keyword(s):  
Corrosion Protection ◽  
Silicon Based

Charge Trapping and Degradation Properties of PZT Thin Films for MEMS

1996 ◽  
Vol 444 ◽  
Author(s):  
Hyeon-Seag Kim ◽  
D. L. Polla ◽  
S. A. Campbell
Keyword(s):  
Thin Films ◽  
Loss Factor ◽  
High Field ◽  
Microelectromechanical Systems ◽  
Charge Trapping ◽  
Metal Organic ◽  
Electrical Reliability ◽  
Silicon Based ◽  
Degradation Properties ◽  
Organic Decomposition

AbstractThe electrical reliability properties of PZT (54/46) thin films have been measured for the purpose of integrating this material with silicon-based microelectromechanical systems. Ferroelectric thin films of PZT were prepared by metal organic decomposition. The charge trapping and degradation properties of these thin films were studied through device characteristics such as hysteresis loop, leakage current, fatigue, dielectric constant, capacitancevoltage, and loss factor measurements. Several unique experimental results have been found. Different degradation processes were verified through fatigue (bipolar stress), low and high charge injection (unipolar stress), and high field stressing (unipolar stress).

Photoconductivity in Vacuum Deposited Films of Silicon-Based Polymers

1996 ◽  
Vol 444 ◽  
Author(s):  
H. Okumoto ◽  
M. Shimomura ◽  
N. Minami ◽  
Y. Tanabe
Keyword(s):  
Charge Transfer ◽  
Chemical Modification ◽  
Electronic Transport ◽  
Hole Transport ◽  
Electron Acceptors ◽  
Oxygen Atmosphere ◽  
Dangling Bonds ◽  
Silicon Based ◽  
Deposited Films

AbstractSilicon-based polymers with σconjugated electrons have specific properties; photoreactivity for microlithography and photoconductivity for hole transport materials. To explore the possibility of combining these two properties to develop photoresists with electronic transport capability, photoconductivity of polysilanes is investigated in connection with their photoinduced chemical modification. Increase in photocurrent is observed accompanying photoreaction of poly(dimethylsilane) vacuum deposited films. This increase is found to be greatly enhanced in oxygen atmosphere. Such changes of photocurrent can be explained by charge transfer to electron acceptors from Si dangling bonds postulated to be formed during photoreaction.

Use of Transparent Conductive Oxide Materials with Low Indices of Refraction in Amorphous Silicon-Based Solar Cell Technology

2005 ◽  
Vol 862 ◽  
Author(s):  
Scott J. Jones ◽  
Joachim Doehler ◽  
Tongyu Liu ◽  
David Tsu ◽  
Jeff Steele ◽  
...  
Keyword(s):  
Solar Cell ◽  
Amorphous Silicon ◽  
Transparent Conductive Oxide ◽  
Open Circuit ◽  
Long Term Stability ◽  
Back Reflectors ◽  
Stability Issues ◽  
Silicon Based ◽  
Solar Cell Technology

AbstractNew types of transparent conductive oxides with low indices of refraction have been developed for use in optical stacks for the amorphous silicon (a-Si) solar cell and other thin film applications. The alloys are ZnO based with Si and MgF added to reduce the index of the materials through the creation of SiO2 or MgF2, with n=1.3-1.4, or the addition of voids in the materials. Alloys with 12-14% Si or Mg have indices of refraction at λ=800nm between 1.6 and 1.7. These materials are presently being used in optical stacks to enhance light scattering by Al/multi-layer/ZnO back reflectors in a-Si based solar cells to increase light absorption in the semiconductor layers and increase open circuit currents and boost device efficiencies. In contrast to Ag/ZnO back reflectors which have long term stability issues due to electromigration of Ag, these Al based back reflectors should be stable and usable in manufactured PV products. In this manuscript, structural properties for the materials will be reported as well as the performance of solar cell devices made using these new types of materials.

Low Power Pixel-Level ADC Readout Circuit for an Amorphous Silicon-Based Microbolometer

2009 ◽  
Vol E92-C (5) ◽  
pp. 708-712
Author(s):  
Dong-Heon HA ◽  
Chi Ho HWANG ◽  
Yong Soo LEE ◽  
Hee Chul LEE
Keyword(s):  
Low Power ◽  
Amorphous Silicon ◽  
Readout Circuit ◽  
Silicon Based
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