Capabilities and disadvantages of combined matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and high-performance thin-layer chromatography (HPTLC): Analysis of egg yolk lipids

2009 ◽  
Vol 22 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Beate Fuchs ◽  
Jürgen Schiller ◽  
Rosmarie Süß ◽  
Ariane Nimptsch ◽  
Martin Schürenberg ◽  
...  
Keyword(s):  
High Performance ◽  
Laser Desorption ◽  
Egg Yolk ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Layer Chromatography ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption ◽  
Combined Matrix

scholarly journals Analysis of Brain Lipids by Directly Coupled Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry and High-Performance Thin-Layer Chromatography

2008 ◽  
Vol 91 (5) ◽  
pp. 1227-1236 ◽  
Author(s):  
Beate Fuchs ◽  
Ariane Nimptsch ◽  
Rosmarie Süß, ◽  
Jürgen Schiller
Keyword(s):  
High Performance ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Brain Lipids ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Layer Chromatography ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Abstract Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a soft ionization MS technique providing only minor fragmentation of the analyte. Therefore, the method is basically suitable for mixture analysis, although the ion yields strongly depend on the basicity/acidity of the analyte in relation to the applied matrix. Accordingly, less sensitively detectable compounds may be suppressed by more sensitively detectable compounds. Thus, separation of the mixture into the individual compounds is normally indispensable. This paper demonstrates the capabilities and limitations of a direct, simple, and inexpensive MALDI-high-performance thin-layer chromatography (HPTLC) coupling for the analysis of a crude lipid extract from porcine brain. Brain lipids were chosen because they represent a rather complex mixture and are of currently significant research interest. It was found that normal-phase HPTLC-separated lipids can be easily characterized by direct MALDI-TOF-MS analysis with sufficient resolution to allow the assignment of virtually all lipid classes, even rather minor species such as phosphorylated phosphoinositides or complex glycolipids as gangliosides. Advantages and disadvantages of this approach are discussed.

scholarly journals Lipidoak aztertu eta identifikatzeko TLC kromatografiaren eta MALDI masa-espektrometriaren arteko akoplamendua

2018 ◽  
pp. 149-165
Author(s):  
Antonio Veloso
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Maldi Ims ◽  
Layer Chromatography ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Geruza meheko kromatografia teknika (TLC, ingelesezko Thin Layer Chromatography) oso erabilia izan da hainbat molekularen banaketa eta karakterizaziorako. Bestalde, Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) edo matrizez lagundutako laserraren bidezko desortzioionizazioa/hegaldi-denbora masa-espektrometria teknikaren erabilera gero eta gehiago hedatzen ari da molekula desberdinen analisia egiteko. Gainera, Imaging Mass Spectrometry (IMS) edo masa-espektrometriaren bidezko irudia izeneko teknikak, ahalbidetzen du ehun biologikoetan dauden lipidoen, proteinen eta farmakoen banaketa-irudiak lortzea eta teknika bikaina da molekula horien banaketa aztertzeko. Teknika honek lipido mota ezberdinen identifikazioa eta lokalizazioa ahalbidetzen du, konposatuaren edota familia kimikoaren aukera egin aurretik. Analizatu behar diren konposatuen aukera aldez aurretik egin behar ez denez, aukera dago irudiak sortzeko masa-espektrometroan detektatutako ioietatik abiatuta. Lan honetan, erakusten da lipidoen ikerkuntzarako oso lagungarria dela TLC xaflak eta MALDI IMS teknikak konbinatzea.

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