Structure of polish isolates ofBipolaris sorokinianaand effect of different pathotypes on spot blotch severity of selected spring barley cultivars

2019 ◽  
pp. 1-10
Author(s):  
M. Cegiełko ◽  
M. Wit ◽  
I. Kiecana ◽  
W. Wakuliński ◽  
E. Mielniczuk
Keyword(s):  
Spring Barley ◽  
Spot Blotch ◽  
Barley Cultivars

Structure of Polish isolates ofBipolaris sorokinianaand effect of different pathotypes on spot blotch severity of selected spring barley cultivars

2019 ◽  
Vol 47 (2) ◽  
pp. 314-323
Author(s):  
M. Cegiełko ◽  
M. Wit ◽  
I. Kiecana ◽  
W. Wakuliński ◽  
E. Mielniczuk
Keyword(s):  
Spring Barley ◽  
Spot Blotch ◽  
Barley Cultivars

scholarly journals The In-Silico Development of DNA Markers for Breeding of Spring Barley Varieties That Are Resistant to Spot Blotch in Russia

Agriculture ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 505
Author(s):  
Irina V. Rozanova ◽  
Nina M. Lashina ◽  
Vadim M. Efimov ◽  
Olga S. Afanasenko ◽  
Elena K. Khlestkina
Keyword(s):  
Dna Markers ◽  
Root Rot ◽  
Spring Barley ◽  
Significant Snps ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
Yield Losses ◽  
Barley Cultivars ◽  
Northwestern Region ◽  
The Impact

The fungal pathogen Cochliobolus sativus Drechs. Ex Dastur, anamorph Bipolaris sorokiniana (Sacc.) Shoemaker is one of the most common barley pathogens worldwide and causes spot blotch and root rot in barley. Spot blotch is considered to be the major biotic stress hampering the commercial production of barley. During high disease severity, which occurs in the northwestern region of Russia once every three to four years, yield losses for barley may reach 40%. An increase in common root rot severity results in yield losses that can reach 80%. The goal of the current study was to identify significant markers that can be employed as diagnostic DNA markers to breed C. sativus pathogen-resistant varieties of barley. In 94 spring barley cultivars and lines, the resistance of seedlings and adult plants to the impact of C. sativus on their leaves and roots was investigated. Five genomic regions associated with resistance to Spot blotch were identified (on chromosome 1H (50–61.2 cM), 2H (68.7–69.68 cM), 3H (18.72–26.18 cM), 7H (7.52–15.44 cM)). No significant loci were determined to be associated with root rot. According to obtained data, 11 significant SNPs were converted into KASP markers and 6 markers located on chromosome 3H were determined to possess good accuracy and the potential to be employed in marker-assisted selection.

Referencing laser and ultrasonic height measurements of barleycultivars by using a herbometre as standard

2016 ◽  
Vol 67 (12) ◽  
pp. 1215 ◽  
Author(s):  
Gero Barmeier ◽  
Bodo Mistele ◽  
Urs Schmidhalter
Keyword(s):  
Plant Height ◽  
Spring Barley ◽  
Operating Time ◽  
Reference Method ◽  
Fold Increase ◽  
Barley Cultivars ◽  
Coefficients Of Variation ◽  
Field Phenotyping ◽  
High Throughput Phenotyping ◽  
Distance Sensor

Assessment of plant height is an important factor for agronomic and breeder decisions; however, current field phenotyping, such as visual scoring or using a ruler, is time consuming, labour intensive, costly and subjective. For agronomists and plant breeders, the most common method used to measure plant height is still a meter stick. In a 3-year study, we have adopted a herbometre similar to a rising plate meter as a reference method to obtain the weighted plant height of barley cultivars and to evaluate vehicle-based ultrasonic and laser distance sensors. Sets of 30 spring barley cultivars and 14 and 60 winter barley cultivars were tested in 2013, 2014 and 2015, respectively. The herbometre was well suited as a reference method allowing for an increased area and was easy to handle. The herbometre measurements within a plot showed very low coefficients of variation. Good and close relationships (R2 = 0.59, 0.76, 0.80) between the herbometre and the ultrasonic distance sensor measurements were observed in the years 2013, 2014 and 2015, respectively, demonstrating also increased values of heritability. Hence, both sensors were able to differentiate among barley cultivars in standard breeding trials. For the sensors, we observed a 4-fold faster operating time and 6-fold increase of measurement points compared with the herbometre measurement. Based on these results, we conclude that distance sensors represent a powerful and economical high-throughput phenotyping tool for breeders and plant scientists to estimate plant height and to differentiate cultivars for agronomic decisions and breeding activities potentially being also applicable in other small grain cereals with dense crop stands. Particularly, ultrasonic distance sensors may reflect an agronomically and physiologically relevant plant height information.

scholarly journals Molecular Mapping of Loci Conferring Susceptibility to Spot Blotch and Resistance to Powdery Mildew in Barley Using the Sequencing-Based Genotyping Approach

2020 ◽  
Vol 110 (2) ◽  
pp. 440-446 ◽  
Author(s):  
Yueqiang Leng ◽  
Mingxia Zhao ◽  
Jason Fiedler ◽  
Antonín Dreiseitl ◽  
Shiaoman Chao ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Molecular Mapping ◽  
Recombinant Inbred Lines ◽  
Major Gene ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
Blumeria Graminis ◽  
Seedling Stage ◽  
Barley Cultivars ◽  
Trait Locus

Spot blotch (SB) caused by Bipolaris sorokiniana and powdery mildew (PM) caused by Blumeria graminis f. sp. hordei are two important diseases of barley. To map genetic loci controlling susceptibility and resistance to these diseases, a mapping population consisting of 138 recombinant inbred lines (RILs) was developed from the cross between Bowman and ND5883. A genetic map was constructed for the population with 852 unique single nucleotide polymorphism markers generated by sequencing-based genotyping. Bowman and ND5883 showed distinct infection responses at the seedling stage to two isolates (ND90Pr and ND85F) of Bipolaris sorokiniana and one isolate (Race I) of Blumeria graminis f. sp. hordei. Genetic analysis of the RILs revealed that one major gene (Scs6) controls susceptibility to Bipolaris sorokiniana isolate ND90Pr, and another major gene (Mla8) confers resistance to Blumeria graminis f. sp. hordei isolate Race I, respectively. Scs6 was mapped on chromosome 1H of Bowman, as previously reported. Mla8 was also mapped to the short arm of 1H, which was tightly linked but not allelic to the Rcs6/Scs6 locus. Quantitative trait locus (QTL) analysis identified two QTLs, QSbs-1H-P1 and QSbs-7H-P1, responsible for susceptibility to spot blotch caused by Bipolaris sorokiniana isolate ND85F in ND5883, which are located on chromosome 1H and 7H, respectively. QSbs-7H-P1 was mapped to the same region as Rcs5, whereas QSbs-1H-P1 may represent a novel allele conferring seedling stage susceptibility to isolate ND85F. Identification and molecular mapping of the loci for SB susceptibility and PM resistance will facilitate development of barley cultivars with resistance to the diseases.

scholarly journals Adaptability of prospective spring barley cultivars bred in Federal Agricultural Research Center of the North-East

2021 ◽  
Vol 36 ◽  
pp. 01013
Author(s):  
Irina N. Shchennikova ◽  
Olga N. Shupletsova ◽  
Irina Yu. Zaytseva ◽  
Larissa P. Kokina ◽  
Rimma I. Vyatkina
Keyword(s):  
Agricultural Research ◽  
Spring Barley ◽  
Culture Method ◽  
Field Studies ◽  
Tube Formation ◽  
Yield Potential ◽  
High Yield ◽  
Barley Cultivars ◽  
North East ◽  
Yield Capacity

Based on the statistical assessment of long-term field studies, in competitive variety tests, barley cultivars were identified adaptive to the conditions of the region, promising for transfer to the State variety test. As a result of the studies, a promising breeding material was created. The efficiency of using various methods of creating a new initial material, in particular, the cell culture method, is shown. A statistically significant correlation was established between the value of the hydrothermal coefficient (GTK) in the period “leaf tube formation - heading” and the yield capacity of a cultivar (r = 0.520). High variability (V = 24.4%) in the duration of the inter-stage period “leaf tube formation - heading” was revealed in the cultivars of competitive variety testing. Cultivars 94-13 and 38-15 are distinguished by a high yield potential (up to 6.4… 6.9 t/ha) and the duration of inter-stage periods, at which the main stages of organogenesis occur at the most favorable combination of heat and moisture for the potential development of plant features and properties. Cultivars 207-15, 38-15, and regenerant 550-08, characterized by high annual average yield capacity (5.9…5.7 tons/ha) and adaptability, are promising for transfer to the Russian Federation State variety test.

scholarly journals Virulence of Rhizoctonia oryzae on Wheat and Barley Cultivars from the Pacific Northwest

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 51-55 ◽  
Author(s):  
T. C. Paulitz ◽  
J. D. Smith ◽  
K. K. Kidwell
Keyword(s):  
Winter Wheat ◽  
Pacific Northwest ◽  
Spring Wheat ◽  
Spring Barley ◽  
Root Diameter ◽  
Natural Soil ◽  
Root Tips ◽  
Barley Cultivars ◽  
Rhizoctonia Oryzae ◽  
Waitea Circinata

Rhizoctonia oryzae (teleomorph = Waitea circinata) causes sheath spot of rice and root rot of wheat and barley. R. oryzae commonly is isolated from barley, wheat, and pea plants in eastern Washington and Idaho. Eight representative isolates were tested for virulence on spring barley (Hordeum vulgare cv. Baronesse), soft white winter wheat (Triticum aestivum cv. Madsen), and hard red spring wheat (cv. Scarlet) planted in natural soil in the greenhouse and maintained at 16°C. All isolates caused significant reduction of emergence in barley, but only seven of the eight isolates and one of the eight isolates reduced emergence of winter wheat and spring wheat, respectively. All isolates caused significant stunting and reduction in the number of seminal roots, root length, and number of root tips on wheat and barley. Some isolates also reduced the frequency of fine secondary roots, resulting in a reduction of the average root diameter. Spring barley was more susceptible to R. oryzae than winter or spring wheat. The main effects of both cultivar and isolate were significant, and there was a significant isolate-cultivar interaction. R. oryzae isolate 80042 was the most virulent on barley, whereas R. oryzae isolate 801387 was the most virulent on wheat. The two isolates from pea were intermediate in virulence on wheat and barley. When screening germ plasm for potential resistance, isolates exhibiting the maximum virulence for each host should be used.

scholarly journals Effect of Fusarium Head Blight on Mycotoxin Content in Grain of Spring Barley Cultivars

2011 ◽  
Vol 40 (No. 3) ◽  
pp. 91-101 ◽  
Author(s):  
V. Šíp ◽  
L. Tvarůžek ◽  
J. Chrpová ◽  
S. Sýkorová ◽  
L. Leišová ◽  
...  
Keyword(s):  
Spring Barley ◽  
The Other ◽  
Cultivar Differences ◽  
Pcr Analysis ◽  
Yield Traits ◽  
Head Blight ◽  
Fungicide Treatment ◽  
Barley Cultivars ◽  
Cultivar Responses ◽  
Moderate Resistance

The results are based on field infection experiments with six spring barley cultivars registered in the Czech Republic and resistance sources Chevron and CI 4196. One of the four Fusarium isolates used for inoculations was a predominant nivalenol producer, while the other isolates were deoxynivalenol (DON) producers. Out of the other mycotoxins 3-AcDON was found in grain at a relatively higher concentration. Significant cultivar differences in DON content, examined yield traits, percentage of Fusarium colonies and percentage of non-germinating seeds were detected after inoculum spraying on two dates and mist irrigation of infected plots. When inoculated with aggressive isolate of F. culmorum Chevron and CI 4196 cvs. showed high resistance and the cultivars Jersey, Olbram and Scarlett moderate resistance to DON accumulation in grain. Treatment with fungicide Horizon 250 EW (active ingredient tebuconazole) led on average to a 52.5% reduction of DON content, but the efficacy of fungicide treatment was highly influenced by year and cultivar. Fungicide treatment did not have a significant effect on grain weight per spike and, in general, the influence of infection on examined yield traits was low in these experiments. DON content was closely related only with the parameter C<sub>T </sub>Fus (transformed) from quantitative real time PCR analysis. Using the developed PCR system it was possible to specify clearly cultivar responses to infection and effects of fungicide treatment on DON content. &nbsp;

scholarly journals Resistance to Fusarium head blight in spring barley

2011 ◽  
Vol 47 (No. 2) ◽  
pp. 58-63 ◽  
Author(s):  
J. Chrpová ◽  
V. Šíp ◽  
L. Štočková ◽  
L. Stemberková ◽  
L. Tvarůžek
Keyword(s):  
Fusarium Head Blight ◽  
Spring Barley ◽  
Fusarium Culmorum ◽  
Grain Weight ◽  
Head Blight ◽  
The Czech Republic ◽  
Disease Symptoms ◽  
Barley Cultivars ◽  
Yield And Quality ◽  
Medium Resistance

Fusarium head blight (FHB) is a fungal disease causing substantial yield and quality losses in barley. Genetic variation in deoxynivalenol (DON) content and and important yield traits in response to FHB were studied in 44 spring barley cultivars for two years following artificial inoculation with Fusarium culmorum under field conditions. The analysis of variance revealed that the largest effect on DON content and simultaneously on the reduction of thousand grain weight and grain weight per spike were due to the environmental conditions of the year, while the visual disease symptoms depended on the cultivars to a larger extent. All these traits were significantly interrelated. The most resistant cultivars Murasski mochi, Nordic, Krasnodarskij 35, Krasnodarskij 95, Nordus, and Usurijskij 8, together with the resistant check Chevron, showed the lowest DON content, the lowest expression of disease symptoms and the lowest reduction of TGW and GWS. However, most spring barley cultivars registered in the Czech Republic in recent years expressed susceptibility or medium resistance and were considerably affected by the disease. This increases the importance of breeding barley for resistance to FHB.

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