Referencing laser and ultrasonic height measurements of barleycultivars by using a herbometre as standard

2016 ◽  
Vol 67 (12) ◽  
pp. 1215 ◽  
Author(s):  
Gero Barmeier ◽  
Bodo Mistele ◽  
Urs Schmidhalter

Assessment of plant height is an important factor for agronomic and breeder decisions; however, current field phenotyping, such as visual scoring or using a ruler, is time consuming, labour intensive, costly and subjective. For agronomists and plant breeders, the most common method used to measure plant height is still a meter stick. In a 3-year study, we have adopted a herbometre similar to a rising plate meter as a reference method to obtain the weighted plant height of barley cultivars and to evaluate vehicle-based ultrasonic and laser distance sensors. Sets of 30 spring barley cultivars and 14 and 60 winter barley cultivars were tested in 2013, 2014 and 2015, respectively. The herbometre was well suited as a reference method allowing for an increased area and was easy to handle. The herbometre measurements within a plot showed very low coefficients of variation. Good and close relationships (R2 = 0.59, 0.76, 0.80) between the herbometre and the ultrasonic distance sensor measurements were observed in the years 2013, 2014 and 2015, respectively, demonstrating also increased values of heritability. Hence, both sensors were able to differentiate among barley cultivars in standard breeding trials. For the sensors, we observed a 4-fold faster operating time and 6-fold increase of measurement points compared with the herbometre measurement. Based on these results, we conclude that distance sensors represent a powerful and economical high-throughput phenotyping tool for breeders and plant scientists to estimate plant height and to differentiate cultivars for agronomic decisions and breeding activities potentially being also applicable in other small grain cereals with dense crop stands. Particularly, ultrasonic distance sensors may reflect an agronomically and physiologically relevant plant height information.

Author(s):  
Zh. M. Novak ◽  

Different weather conditions provide unequal growth and development of plants, in addition, biotypes react differently to changes in external environmental factors. Scientists from Ukraine and other countries analyze varieties and samples of spring barley for the purpose of determine donors of useful traits in different soil and climatic conditions. For the purpose of combine a number of useful traits in one genotype, hybridization between geographically distant biotypes is used. Uman National University of Horticulture has a collection of spring barley samples by different geographic origin. The analyze of breeding material by biometric traits, which will allow us to determine the level of manifestation of each of them in different conditions of 2018–2020 and to determine the donors of useful traits for the further breeding process, was carried out. The weather conditions of the years of research were very different. 2018 was the most unfavorable year for early spring crops. The reason for this was the presence of snow cover until the end of March. After that were strong heat and lack of precipitation. 2019 and 2020 were more favorable for spring barley due to the long cool spring. In addition, in 2020, May and June characterized by increased rainfall. The average plant height of the studied biotypes ranged from 50 to 64 cm. Most samples had the lowest plant height 40–57 cm in 2018, and the highest – in 2020 – from 54 to 88 cm. The average spike length of the analyzed samples was 5.9–8.1 cm. There was not dependence between conditions of the research year and spike length. The number of spikelets per spike was naturally determined by the research conditions. The lowest indicators were observed in 2018 — from 7.0 to 12.0 units, while in 2019 — 16.6–23.9 units, and in 2020 — 20.2–27.7 units. The variation of plant height of the analyzed samples was medium and significant with coefficients from 13.7 to 32.6 %. There was a slight, medium and significant variation of the length of the ear (V = 2.9–23.6 %). The index of number of spikelets per spike most varied — the coefficients of variation were 32.6–55.5 %.


1970 ◽  
Vol 50 (1) ◽  
pp. 97-101 ◽  
Author(s):  
GARRY A. SMITH ◽  
WILSON H. FOOTE

The effect of heterozygosity on the phenotypic stabilities of tiller number, plant height, kernels per spike, kernel weight, grain yield, and awn length was studied in hybrid crosses of six-row barley Hordeurn vulgare L. emend Lam., and two-row barley Hordeum distichum L. emend Lam. Experimental material consisting of parents and their F1 diallel progeny were grown at two field locations and one greenhouse location in Oregon. Coefficients of variation were used to measure phenotypic stability within and across the three locations.Some evidence was obtained which indicated that heterozygosity reduced phenotypic stability of particular characters at some locations. Although individual characters were affected within particular locations, heterozygosity per se did not influence average phenotypic stability when the results from three diverse environments were combined.Association studies indicated that the phenotypic stability of kernel weight, plant height, kernels per spike and awn length were associated, which may suggest a common control mechanism in the phenotypic stability of these characters.


Author(s):  
Zh. M. Novak ◽  

The study of crop formation processes by constituent elements revealed the dependence of the parameters of the number of grains in the ear and the weight of barley grain at the same level of grain yield on the number of productive stems per unit area. It is established that the increase in the number of productive shoots per 1 m2 is accompanied by a significant decrease in the productivity potential of the ear in terms of the number of grains and the parameters of the mass of one grain. The correlation dependence of these yield elements on the stem density of barley is strong. In the studies of 2018–2021, the number of productive stems, plant height, productivity of one ear and weight of 1000 grains of spring barley varieties Daniele, Gezine, Beatrix, Soldo, 5/18, Fabiola, Sangria, Utah, 9/19, Mompi 19, Lyuba and Champush. Correlation holidays between these indicators were also established. The number of productive stems of spring barley varieties on average for 2018–2021 amounted to 0.87–1.39 pieces/1 plant. The average biotype ranged from 0.89 in 2018 to 1.47 in 2021. There was a medium and strong variation in the number of productive stems depending on genotypes. In most collection specimens, the rate varied greatly depending on growing conditions. Collectible samples 5/18 is a semi-dwarf, other biotypes are dwarfs. The highest plants were in 2020. The plant height of most cultivars varied slightly over the years of research. The average productivity of one ear of the analyzed collection samples was 0.63–1.17 g. The lowest indicators were noted in 2018, the highest – in 2020. The average weight of 1000 grains was 45.2–53.5 g with the highest indicator in 2021 g. The correlation between plant height and ear weight per ear was positive medium and close; between the number of productive stems and plant height – positive average, weak and negative average; between the number of productive stems and the mass of grain from the ear – a weak positive and negative correlation; between the number of productive stems and the mass of 1000 grains – the average positive and weak negative; between the height of the plants and the mass of 1000 grains and between the mass of grains from the ear and the mass of 1000 grains – from the average positive to the average negative.


2016 ◽  
Vol 8 (12) ◽  
pp. 1031 ◽  
Author(s):  
Fenner Holman ◽  
Andrew Riche ◽  
Adam Michalski ◽  
March Castle ◽  
Martin Wooster ◽  
...  

2018 ◽  
Vol 27 (4) ◽  
pp. 250-5
Author(s):  
Sultan A.M. Saghir ◽  
Amer A. Almaiman ◽  
Aishah K.A. Shatar ◽  
Norris Naim ◽  
Huda S. Baqir

Background: The fast and outpatient setting for a determination of the hemoglobin (Hb) level is a well-recognized prerequisite to detect anemia in blood donors. This study aimed to evaluate the performance of the HemoCue methods (HemoCue B-Hb and HemoCue-301) against Coulter LH-750 as a reference method for Hb determination.Methods: This study was an experimental cross-sectional study. It includes 455 blood samples that were collected from volunteer blood donors between January 15, 2010 and February 15, 2011. The performance of the three methods and their comparisons were assessed using the analysis of coefficients of variation (CV), linear regression, and mean difference. Correlation coefficient and Bland–Altman plots were drawn to compare the two HemoCue measurements and the automated cell analyzer against each other and to evaluate their results. The Hb concentrations were compared using the concordance correlation coefficient.Results: The findings exhibited that the CV for the three methods Coulter LH-750, HemoCue B-Hb, and HemoCue-301 were 0.60%, 0.72%, and 0.92%, respectively. A statistically significant difference was observed between the means of the Hb measurements for the three methods (p<0.001). The HemoCue B-Hb and HemoCue-301 methods showed the best agreement, and the Coulter LH-750 method gave a lower Hb value compared with the two HemoCue methods. The results showed a positive correlation of HemoCue Hb results compared with the reference method.Conclusion: All three methods provide a good agreement for Hb determination. The new device HemoCue-301 was found to be more accurate compared with HemoCue B-Hb and Coulter LH-750.


2020 ◽  
Vol 47 (3) ◽  
pp. 275-282
Author(s):  
Dewi Andriani ◽  
Desta Wirnas ◽  
Dan Trikoesoemaningtyas

Sorghum has a growing popularity for food, feed, biofuel, and therefore needs to be developed. The research aimed to compare the effectiveness of pedigree and modified bulk selection methods for improving yield in three sorghum populations. The genetic materials used were 60 F6 lines from three populations, namely PI 10-90-A x Numbu, PI 150-20-A x Numbu and PI 150-20-A x Kawali which had been selected using pedigree and modified bulk methods. The experiment was conducted from October 2018 to February 2019 at the Cikabayan Experimental Station of IPB University, Dramaga, Bogor. The experiment was arranged in an augmented design with six checks, namely Kawali, Numbu, PI 10-90-A, PI 150-20-A, Samurai 1, and Samurai 2 which were replicated four times. Observations were made on agronomic traits and yield. Plant height and grain weight per panicle had high heritability and large genotypic coefficients of variation. The contrast test results showed significant differences between populations in plant height, panicle length, days of harvesting and 100-seed weight. On the other hand, no significant difference was observed among selection methods, indicating that both methods were equally effective for increasing sorghum yield. The pedigree selection and modified bulk selection increased grain yield per panicle by 14.1 g and 18.2 g respectively. Bulk of the best genotypes in early generation could be an alternative of bulk selection method. Keywords: contrast test, differential selection, genotypic coefficient of variation, heritability


Genetika ◽  
2012 ◽  
Vol 44 (3) ◽  
pp. 499-512 ◽  
Author(s):  
Novo Przulj ◽  
Vojislava Momcilovic

Environmental conditions in the Pannonian zone can be characterized with moderate high temperature and partially water deficit during grain filling of spring barley, although low temperature and water deficit are possible also in period till anthesis. This study was conducted to evaluate the variation of the duration of the period from emergence to anthesis (VP), duration of grain filling period (GFP), plant height (PH), spikes number m-2 (SN), grains number spike-1 (GN), thousand grains weight (GW) and yield (YIL) in spring two-rowed barley in conditions of the Pannonian zone. All three factors; genotype, environment and the interaction GxY affected the studied traits. Average VP was 777 GDD, GFP 782 GDD, PH 78 cm, SN 523, GN 28.2, GW 43.2 g and YIL 6.26 t ha-1. Variation across varieties was higher than across growing seasons. Heritability varied from 0.66 for YIL to 0.94 for VP and GFP. This study confirmed that a sufficiently large genetic variability must be base for selecting appropriate varieties for the Pannonian zone conditions. In order to determine high yielding and quality barley extensive research in relation to breeding, variety choice for production and growing practice must be done.


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