scholarly journals Using markers and field evaluation to identify the source of eyespot resistance genePch1in the collection of wheat breeding lines

2015 ◽  
Vol 43 (4) ◽  
pp. 638-648 ◽  
Author(s):  
M. Kwiatek ◽  
H. Wiśniewska ◽  
Z. Kaczmarek ◽  
M. Korbas ◽  
M. Gawłowska ◽  
...  
Keyword(s):  
Field Evaluation ◽  
Wheat Breeding ◽  
Breeding Lines ◽  
Eyespot Resistance

scholarly journals Identification of PCH1 Eyespot Resistance Gene in the Collection of Wheat Lines (Triticum Aestivum L.)

2012 ◽  
Vol 52 (2) ◽  
pp. 254-258 ◽  
Author(s):  
Michał Kwiatek ◽  
Katarzyna Pankiewicz ◽  
Halina Wiśniewska ◽  
Marek Korbas ◽  
Jakub Danielewicz
Keyword(s):  
Triticum Aestivum ◽  
Resistance Gene ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Field Conditions ◽  
Sts Marker ◽  
Breeding Lines ◽  
Eyespot Resistance ◽  
Resistant Lines ◽  
Wheat Lines

Identification of PCH1 Eyespot Resistance Gene in the Collection of Wheat Lines (Triticum AestivumL.)Endopeptidase markerEpD1band STS markerXustSSR2001-7DLare closely linked to very effective eyespot resistance genePch1. Because of this, the aim of this study was to compare the results obtained under lab conditions using such markers with the results obtained under field conditions. 134 wheat breeding lines andTriticum aestivumL. var. Randevous used as a eyespot resistance control were analized. The combination of three methods allowed to select eight completely resistant or high resistant lines, that could be used in following breeding processes. Results obtained using endopeptidase and STS markers in 100% correlate with the phenotyping scoring.

scholarly journals How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

2016 ◽  
Vol 56 (4) ◽  
pp. 319-322 ◽  
Author(s):  
Michał Kwiatek ◽  
Halina Wiśniewska ◽  
Marek Korbas ◽  
Magdalena Gawłowska ◽  
Jolanta Belter ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Wheat Variety ◽  
Wheat Breeding ◽  
Aegilops Ventricosa ◽  
Wheat Varieties ◽  
Breeding Lines ◽  
Eyespot Resistance ◽  
Limited Effectiveness ◽  
Wheat Lines ◽  
Resistance Expression

Abstract Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez; (3) the Pch3 gene, originating from Dasypyrum villosum; and (4) the Q.Pch.jic-5A gene, a quantitative trait locus (QTL) located on chromosome 5A of Capelle Desprez. However, those loci have drawbacks, such as linkage of Pch1 with deleterious traits and limited effectiveness of Pch2 against the disease. Here we present an initial study which aims to characterize wheat pre-registration breeding lines carrying 12 eyespot resistance genes, consider their resistance expression in inoculation tests and the influence of resistance genotypes on the yield. We selected four groups of breeding lines, carrying: (1) the Pch1 gene alone: one line; (2) the Pch2 gene alone: four lines; (3) the Q.Pch.jic-5A gene alone: one line; and (4) Pch1 + Q.Pch.jic-5A: three lines. For the first time, the effect of the combination of Pch1 and Q.Pch.jic-5A genes was compared with resistance conferred by Pch1 or Q.Pch.jic-5A alone. We found significant differences between infection scores evaluated in resistant lines carrying Pch1 and Q.Pch.jic-5A alone, while no differences in terms of the level of resistance expression were detected between Pch1 alone and Pch1 + Q.Pch.jic-5A, and between wheat lines carrying Pch1 and Pch2 alone. Moreover, we demonstrated that the Pch1 gene, together with an Ae. ventricosa segment, caused statistically significant yield losses, both as a single eyespot resistance source or in a combination with Q.Pch.jic-5A. Yield scores showed that wheat lines with Q.Pch.jic-5A had the highest yields, similar to the yielding potential of Pch2-bearing lines and control varieties.

scholarly journals Reaction of winter wheat genotypes to infection by Mycosphaerella graminicola(Fuckel) Schroeter

2013 ◽  
Vol 55 (1) ◽  
pp. 233-246
Author(s):  
Ewa Mirzwa-Mróz ◽  
Czesław Zamorski
Keyword(s):  
Winter Wheat ◽  
Broad Spectrum ◽  
Mycosphaerella Graminicola ◽  
Wheat Breeding ◽  
Septoria Tritici ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Wheat Genotypes ◽  
Winter Wheat Cultivars

The response of Polish winter wheat genotypes to <i>M.graminicola</i> (preliminary experiments and cultivar collections) was observed in different regions of Poland. Observations were carried out in 1995-1999. The winter wheat genotypes showed a broad spectrum of reaction to this pathogen. Between 1997 and 1999 the highest degree of infection on winter wheat breeding lines was noted in Kończewice. During this time no genotypes free from infection were observed (preliminary breeding experiments). Cultivars with no symptoms of <i>Septoria tritici</i> blotch (Leszczyńska Wczesna and Żelazna) were found among old genotypes in Słupia Wielka only in earlier experiments (1995-1996). In the years 1997-1999 the winter wheat cultivars were classified into groups on the basis of their response to the pathogen. The degree of infection for the majority cultivars was quite high.

scholarly journals Field Evaluation of Yield and Yield Component Traits of Breeding Lines of Maize over Two Seasons in Derived Savannah Agro-Ecology

2018 ◽  
Vol 10 (4) ◽  
pp. 567-574
Author(s):  
Charles U. UBA ◽  
Christian U. AGBO ◽  
Uchechukwu P. CHUKWUDI ◽  
Andrew A. EFUSIE ◽  
Stella O. MUOJIAMA
Keyword(s):  
Grain Yield ◽  
Block Design ◽  
Field Evaluation ◽  
Yield Component ◽  
Seasonal Effects ◽  
International Institute ◽  
Early Season ◽  
Breeding Lines ◽  
Late Season ◽  
Positive Effects

The understanding of yield and the interaction with its components is very important for selection in early generations of crop breeding. Twelve maize genotypes were collected from International Institute for Tropical Agriculture (IITA) along with seven landraces in order to identify the contribution of different traits to yield improvement. The experiments were carried out in two different seasons (March/April-early and July/August- late) in a randomized complete block design with three replications. Early season planting had a higher grain yield than late season planting. The difference in grain yield between early and late season was 3.92 tons/ha. This represents a 27.8% increase in grain yield during the early season over the late season planting. Number of ears per plant and shelling percentage were not influenced by seasonal effects. Ear weight and days to tasselling showed the highest direct positive effects of 0.972 and 0.665, respectively on grain yield, during early season. Furthermore, ear weight, followed by shelling percentage, exerted the highest direct positive effect on grain yield in late season. Higher indirect positive effects were obtained for ear diameter, ear length, ear height and plant height via ear weight in both seasons. Ear weight, days to tasselling and ear length were identified as the major traits affecting yield of maize in both seasons in the derived Savannah agro-ecology.

Isolation of differentially expressed genes in wheat caryopses with contrasting starch granule size

2013 ◽  
Vol 8 (3) ◽  
pp. 297-305
Author(s):  
Rita Armonienė ◽  
Kristina Jonavičienė ◽  
Vytautas Ruzgas ◽  
Gintaras Brazauskas
Keyword(s):  
Gene Expression ◽  
Winter Wheat ◽  
Differentially Expressed Genes ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Starch Granule ◽  
Wheat Breeding ◽  
Granule Size ◽  
Differentially Expressed ◽  
Breeding Lines

AbstractIn order to identify genes responsible for starch granule initiation during early development of wheat caryopsis, nine winter wheat breeding lines were studied. Two breeding lines, which are the most diverse in A-type granule size (26.85 µm versus 23.65 µm) were chosen for further differential gene expression analysis in developing caryopses at 10 and 15 days post-anthesis (DPA). cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis resulted in 384 transcript-derived fragments, out of which 18 were identified as being differentially expressed. Six differentially expressed genes, together with the six well-known starch biosynthesis genes, were chosen for semi-quantitative gene expression analysis in developing wheat caryopses at 10 and 15 DPA. This study provides genomic information on 18 genes differentially expressed at early stages of wheat caryopses development and reports on the identification of genes putatively involved in the production of large A-type granules. These genes are targets for further validation on their role in starch granule synthesis control and provide the basis for the development of DNA marker tools in winter wheat breeding for enhanced starch quality.

scholarly journals Emerging Yr26-Virulent Races of Puccinia striiformis f. tritici Are Threatening Wheat Production in the Sichuan Basin, China

Plant Disease ◽  
2015 ◽  
Vol 99 (6) ◽  
pp. 754-760 ◽  
Author(s):  
D. J. Han ◽  
Q. L. Wang ◽  
X. M. Chen ◽  
Q. D. Zeng ◽  
J. H. Wu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Sichuan Basin ◽  
Puccinia Striiformis ◽  
Field Tests ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Breeding Lines ◽  
Wheat Production ◽  
Stripe Rust Resistance Gene ◽  
The Sichuan Basin

Stripe rust, caused by Puccinia striiformis f. tritici, is one of the most destructive diseases of wheat in the world. The Sichuan Basin is one of the most important regions of wheat production and stripe rust epidemics in China. Stripe rust resistance gene Yr26 (the same gene as Yr24) has been widely used in wheat breeding programs and in many cultivars grown in this region since the gene was discovered in the early 1990s. Virulence to Yr26 has increased in frequency since its first detection in 2008. The objective of this study was to assess the vulnerability of the wheat cultivars and breeding lines in the Sichuan Basin to Yr26-virulent races. In total, 85 wheat accessions were tested with Yr26-avirulent races CYR32, CYR33, and Su11-4 and two Yr26-virulent races, V26/CM42 and V26/Gui22. DNA markers for Yr26 were used to determine the presence and absence of Yr26 in the wheat accessions. Of the 85 wheat accessions, only 5 were resistant and 19 susceptible to all races tested, and the remaining 61 were resistant to at least one or more races tested in seedling stage. In all, 65 (76.5%) accessions were susceptible to the emerging Yr26-virulent race V26/Gui22. In field tests, susceptible accessions increased from 31.8% in a nursery inoculated with predominant and Yr26-avirulent races to 61.2% in the nursery inoculated with the predominant races mixed with V26/Gui22. Based on the results of the molecular marker and race tests, 33 (38.8%) accessions were determined to have Yr26, showing that the Yr26 virulence is a major threat to wheat production in the Sichuan Basin and potentially in other regions of China.

Genetics of late maturity α-amylase in a doubled haploid wheat population

2010 ◽  
Vol 61 (2) ◽  
pp. 153 ◽  
Author(s):  
M. K. Tan ◽  
A. P. Verbyla ◽  
B. R. Cullis ◽  
P. Martin ◽  
A. W. Milgate ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Value Added ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Breeding Lines ◽  
Positive Effects ◽  
Wheat Population ◽  
B1 Gene ◽  
Late Maturity ◽  
First Time

Late maturity α-amylase (LMA) in wheat is a defect where high-isoelectric point (pI) α-amylase accumulates in the ripening grain. Wheat genotypes vary in expression from zero to high levels of α-amylase, the latter with detrimental consequences on their use for value-added end products. Expression in each genotype is characterised by varying numbers of grains affected and different levels in each grain. Analysis of a doubled haploid (DH) population (188 lines) from WW1842 × Whistler has identified significant QTL on chromosomes 2DL, 3A, 3B, 3D, 4B, 4D, 5DS and 5BL. The 4B LMA allele (P < 0.0001) from Whistler is closely linked to the QTL for the ‘tall’ allele (P < 0.0001) of the Rht-B1 gene. The 4D LMA QTL (P < 0.0001) in WW1842 co-locates with the QTL for the ‘tall’ allele (P < 0.0001) of the Rht-D1 gene. This study has shown for the first time that a DH cross between two semi-dwarf cultivars with low or no LMA produces ~25% of progeny lines of the ‘tall’ genotypes with a high frequency of LMA. This is attributed to the large additive positive effects from the combination of one recessive ‘tall’ Rht-B1 gene and one recessive ‘tall’ Rht-D1 gene. High-yielding semi-dwarf genotypes with different combinations of Rht-B1 and Rht-D1 alleles which have very low or non-existent LMA expression (e.g. WW1842 and Whistler) may meet industry criteria for registration as commercial wheat varieties. However, when they are used as breeding lines, the cross produces some progeny genotypes with severe levels of LMA. These LMA genotypes comprise the gibberellic acid-sensitive ‘tall’ progenies and a very small proportion of semi-dwarfs. Thus, it is of paramount importance to screen the defect in wheat breeding programs. The suite of QTL identified for LMA will enable the use of marker assisted selection in the pyramiding of the beneficial QTL to maximise yield and minimise (or eliminate) LMA in semi-dwarf genotypes.

Photoperiod and vernalization gene effects in southern Australian wheat

2010 ◽  
Vol 61 (9) ◽  
pp. 721 ◽  
Author(s):  
H. A. Eagles ◽  
Karen Cane ◽  
Haydn Kuchel ◽  
G. J. Hollamby ◽  
Neil Vallance ◽  
...  
Keyword(s):  
Field Experiments ◽  
Allelic Variation ◽  
Wheat Breeding ◽  
Gene Effects ◽  
Cross Prediction ◽  
Breeding Lines ◽  
Vrn Genes ◽  
Days To Heading ◽  
Australian Wheat ◽  
Vernalization Genes

Photoperiod and vernalization genes are important for the optimal adaptation of wheat to different environments. Diagnostic markers are now available for Vrn-A1, Vrn-B1, Vrn-D1 and Ppd-D1, with all four genes variable in southern Australian wheat-breeding programs. To estimate the effects of these genes on days to heading we used data from 128 field experiments spanning 24 years. From an analysis of 1085 homozygous cultivars and breeding lines, allelic variation for these four genes accounted for ~45% of the genotypic variance for days to heading. In the presence of the photoperiod-insensitive allele of Ppd-D1, differences between the winter genotype and genotypes with a spring allele at one of the genes ranged from 3.5 days for Vrn-B1 to 4.9 days for Vrn-D1. Smaller differences occurred between genotypes with a spring allele at one of the Vrn genes and those with spring alleles at two of the three genes. The shortest time to heading occurred for genotypes with spring alleles at both Vrn-A1 and Vrn-D1. Differences between the photoperiod-sensitive and insensitive alleles of Ppd-D1 depended on the genotype of the vernalization genes, being greatest when three spring alleles were present (11.8 days) and least when the only spring allele was at Vrn-B1 (3.7 days). Because of these epistatic interactions, for the practical purposes of using these genes for cross prediction and marker-assisted selection we concluded that using combinations of alleles of genes simultaneously would be preferable to summing effects of individual genes. The spring alleles of the vernalization genes responded differently to the accumulation of vernalizing temperatures, with the common spring allele of Vrn-A1 showing the least response, and the spring allele of Vrn-D1 showing a response that was similar to, but less than, a winter genotype.

Field evaluation of early vigour for genetic improvement of grain yield in wheat

2002 ◽  
Vol 53 (10) ◽  
pp. 1137 ◽  
Author(s):  
T. L. Botwright ◽  
A. G. Condon ◽  
G. J. Rebetzke ◽  
R. A. Richards
Keyword(s):  
Grain Size ◽  
Grain Yield ◽  
Biomass Production ◽  
Field Evaluation ◽  
Field Trials ◽  
Wheat Breeding ◽  
Growth And Yield ◽  
Seeding Density ◽  
Recurrent Parent ◽  
Early Vigour

Improved early vigour in wheat (Triticum aestivum L.) has been proposed as an important trait for increasing grain yield through greater water-use efficiency in rainfed, Mediterranean-type environments. Three years of field trials were undertaken in Western Australia at 2 sites, Merredin (low rainfall, 244 mm in the growing season) and Wongan Hills (medium rainfall, 308 mm), to examine the influence of increased early vigour on crop growth and yield. The effect of breeding for greater early vigour was tested in 1998 and 1999 using 3 high vigour and 3 low vigour BC2:F5 lines of the cultivar Amery backcrossed to a 'high vigour' donor. Averaged across environments, the high vigour backcross lines had a 10% increase in early vigour (i.e. leaf area/plant) at 50 DAS compared with the low vigour lines. Differences in yield across environments were associated with variation in total rainfall, rainfall distribution, and soil properties. In the wetter of the 2 years (1999), greater early vigour translated to increased yield of c. 12%, averaged across environments, but there was no difference in yield in either environment in the drier year (1998). Potential deleterious effects of the recurrent parent on yield were eliminated in field trials in 1999 and 2000 by manipulating early vigour through varying grain size (25, 35, or 50 mg) or seeding density (50, 200, or 400 plants/m2) of Amery at sowing. Large grain increased the embryo size and early vigour at 50 DAS in both environments in 1999. This translated to greater biomass production at anthesis and maturity to increase grain yield at Wongan Hills in 1999. In contrast, there was no relationship between grain size, biomass production, and yield at Merredin in 1999 or at either site in 2000. Sowing density treatments also had no effect on yield in 2000. In conclusion, there is potential to increase yield of wheat by selecting for greater early vigour in a wheat breeding program. The expression of vigour in field conditions and the translation of this improvement to higher yields is, however, dependent on the environment. Current yield limitations arising through backcrossing with a high vigour, yet poor yielding donor, need to be addressed.

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