scholarly journals Evaluation of classical and rapid methods for isolation and identification of Mycobacterium bovis in cattle in Bulgaria

2021 ◽  
Vol 24 (3) ◽  
pp. 334-343
Author(s):  
T. Savova-Lalkovska ◽  
M. Bonovska ◽  
A. Dimitrova ◽  
V. Valcheva ◽  
Y. Petkov ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Economic Impact ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Bacterial Isolates ◽  
Tuberculosis Complex ◽  
Isolation And Identification ◽  
Rapid Methods ◽  
Diagnostic Capabilities

Bovine tuberculosis is still a serious problem with major economic impact in many countries. The aim of study was to evaluate the diagnostic capabilities of the classical and some modern, rapid methods for isolation and identification of Mycobacterium bovis. In the period 2015-2018 from 29 outbreaks in 10 different regions of Bulgaria, 1193 lymph nodes from slaughtered cattle were examined by pathoanatomical, bacteriological, PCR and immunochromatographic methods. Of the 283 bacterial isolates, 263 were identified as M. bovis - member of the M. tuberculosis complex.

scholarly journals Distribution of Lesions in Cattle Infected with Mycobacterium Bovis

1996 ◽  
Vol 8 (3) ◽  
pp. 351-354 ◽  
Author(s):  
Diana L. Whipple ◽  
Carole A. Bolin ◽  
Janice M. Miller
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Dairy Herd ◽  
The United States ◽  
Primary Method ◽  
Thoracic Region ◽  
Gross Lesions ◽  
Tissue Specimens ◽  
Postmortem Examinations

Detailed postmortem examinations were conducted on 30 cattle from a dairy herd with bovine tuberculosis to determine the distribution of lesions in Mycobacterium bovid-infected cattle. Twenty-four different tissue specimens from each animal were examined for gross lesions and collected for bacteriologic culturing and histologic examination. Tuberculosis was confirmed in 15 cattle with evidence of infection in 1 or more of the following tissues: medial retropharyngeal, parotid, tracheobronchial, mediastinal, caudal deep cervical, and subiliac lymph nodes; palatine tonsil; and lung. Gross and histologic lesions were present most frequently in lymph nodes of the thoracic region. Mycobacterium bovis was isolated from 3 cattle that had no gross lesions of tuberculosis. One animal had lesions only in the subiliac lymph node, which is not routinely examined during slaughter surveillance. Results of this study indicate that not all cattle infected with M. bovis have visible lesions of tuberculosis in sites that are routinely inspected. These findings are important because detection of gross lesions of tuberculosis during inspection of carcasses at slaughter is the primary method for detection of tuberculous cattle and herds in the United States.

scholarly journals Isolation nontuberculous mycobacteria and histopathological changes in lymph nodes collected at the abattoir in cattle reactive-positive to tuberculin dermal test

2020 ◽  
Author(s):  
Angélica María Hernández Jarguín ◽  
Julio Martínez-Burnes ◽  
Gloria M. Molina-Salinas ◽  
Ned I. de la Cruz-Hernández ◽  
José L. Palomares-Rangel ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Bovine Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Nontuberculous Mycobacteria ◽  
Histopathological Changes ◽  
Tuberculosis Complex ◽  
Confounding Effect ◽  
Granulomatous Lymphadenitis ◽  
The Common ◽  
Abattoir Inspection

Abstract Background: The Mycobacterium tuberculosis complex causes a variety of diseases; in bovine, the common pathogen is M. bovis which is considered zoonotic. A separate group of mycobacteria, much less known, is "non-tuberculous mycobacteria (NTM) which are also infectious for animals and humans. The Mexican Official Norm (NOM-ZOO-031-1995) regulates M. bovis in cattle, but not NTM species, even though this last type of microorganisms has a confounding effect for the diagnosis of bovine tuberculosis. The objective of the study was to isolate and identify the NTM of bovine lymph nodes condemned in the slaughterhouse, to characterize the histological lesions in these tissues and to correlate bacteriological and postmortem findings with the antemortem skin test of the tuberculin. Results: Mycobacteria were isolated from 54/528 (10.2%) of the lymph nodes; 29/54 (53.7%) of these isolates were identified as M. bovis and 25/54 (46.2%) as NTM; 3 bacteriological cultures were discarded due to contamination with fungi and in one case it was not possible to identify the species. Granulomatous and pyogranulomatous inflammation were observed in 6/21 (28.6%) and 7/21 (33.3%) of the NTM-positive lymph nodes, respectively. Necrosis and mineralization were only found in 6/21(28.6%) of the lymph nodes. The species of NTM associated with granulomatous lymphadenitis were M. scrofulaceum, M. triviale, M. terrae and M. szulgai, while those causing pyogranulomatous lesions were M. szulgai, M. kansasii, M. phlei, and M. scrofulaceum. Conclusions: Considering the increase of mycobacterial infections in humans worldwide, the idetification of NTM that inducing Tuberculosis-like lesions in abattoir inspection is the first step to investigate the livestock-human-wildlife-environment interactions with especially focus on transmission dynamics.

scholarly journals Tuberculosis in Antelopes in a Zoo in Poland – Problem of Public Health

2015 ◽  
Vol 64 (4) ◽  
pp. 395-397 ◽  
Author(s):  
Monika Krajewska ◽  
Michał Załuski ◽  
Anna Zabost ◽  
Blanka Orłowska ◽  
Ewa Augustynowicz-Kopeć ◽  
...  
Keyword(s):  
Public Health ◽  
Infectious Disease ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Wild Animals ◽  
Tuberculosis Complex ◽  
Mycobacterium Caprae ◽  
In Captivity

Bovine tuberculosis is an infectious disease that occurs in many species of both domestic and wild animals, as well as those held in captivity. The etiological factor is the acid resistant bacillus (Mycobacterium bovis or Mycobacterium caprae), which is characterized by the major pathogenicity among mycobacteria belonging to the Mycobacterium tuberculosis complex. The material from 8 antelopes from the zoo, suspected for tuberculosis were examined, and M. bovis strains were isolated from 6 of them. The spoligotyping method showing spoligo pattern 676763777777600. In Poland, this spoligotype has not been observed so far.

scholarly journals Bovine tuberculosis in domestic and wild mammals in an area of Dorset. III. The prevalence of tuberculosis in mammals other than badgers and cattle

1982 ◽  
Vol 89 (2) ◽  
pp. 225-234 ◽  
Author(s):  
T. W. A. Little ◽  
C. Swan ◽  
H. V. Thompson ◽  
J. W. Wilesmith
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Rattus Norvegicus ◽  
Bovine Tuberculosis ◽  
Vulpes Vulpes ◽  
Wild Mammal ◽  
Wild Mammals ◽  
Large Sample

SUMMARYA large sample of the wild mammals found on a farm in South Dorset were trapped and examined for the presence ofMycobacterium bovisfollowing the discovery of widespread infection in cattle and badgers.M. boviswas isolated from the lymph nodes of two out of 90 rats (Rattus norvegicus) and one out of seven foxes (Vulpes vulpes) but no lesions of tuberculosis were observed. It was concluded that the badger was the only species of wild mammal which was a reservoir ofM. bovisin this area.

scholarly journals Bovine Tuberculosis in the Republic of Macedonia: Postmortem, Microbiological and Molecular Study in Slaughtered Reactor Cattle

2017 ◽  
Vol 40 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Iskra Cvetkovikj ◽  
Slavcho Mrenoshki ◽  
Kiril Krstevski ◽  
Igor Djadjovski ◽  
Branko Angjelovski ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Molecular Identification ◽  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Nucleotide Polymorphisms ◽  
Republic Of Macedonia ◽  
Mycobacterium Caprae ◽  
Lowenstein Jensen ◽  
The Republic

Abstract Bovine tuberculosis is a chronic infectious disease in cattle caused mainly by Mycobacterium bovis and to a lesser extent by Mycobacterium caprae. The other members of the Mycobacterium tuberculosis complex (MTBC) can also cause the disease in domestic and wild animals and all of them have a zoonotic potential. The main purpose of the study was to determine the presence and distribution of the tuberculous lesions in reactor cattle, and to isolate and identify the causative agents of bovine tuberculosis in the Republic of Macedonia. Lymph nodes and affected organs from 188 reactor cattle slaughtered due to a positive intradermal comparative cervical tuberculin test were analyzed by detection of tuberculous lesions, followed by isolation and molecular identification of the isolated mycobacteria. The isolation was performed on selective media - Lowenstein Jensen with glycerol, Lowenstein Jensen without glycerol and Stonebrink medium supplemented with pyruvate. The molecular identification of the MTBC members was performed by analysis of the Regions of difference (RD1, RD9 and RD4) and detection of single nucleotide polymorphisms in the lepA gene for Mycobacterium caprae. Typical tuberculous lesions were detected in 62 animals (33.0%) and the lesions were most prevalent in the mediastinal lymph nodes (47.5%). The isolated mycobacteria in the MTBC were identified as Mycobacterium bovis and Mycobacterium caprae and were found in both animals with visible lesions (82.2%) and animals without visible lesions (27.7%). The slaughterhouse postmortem examinations and laboratory investigations should be included on regular bases in order to improve the National eradication program.

scholarly journals Gross and Microscopic Lesions of Naturally Occurring Tuberculosis in a Captive Herd of Wapiti (Cervus Elaphus Nelsoni) in Colorado

1992 ◽  
Vol 4 (4) ◽  
pp. 428-433 ◽  
Author(s):  
Jack C. Rhyan ◽  
Dennis A. Saari ◽  
Elizabeth S. Williams ◽  
Michael W. Miller ◽  
Arthur J. Davis ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Cervus Elaphus ◽  
Bovine Tuberculosis ◽  
Caseous Lymphadenitis ◽  
Infected Herd ◽  
Cervus Elaphus Nelsoni ◽  
Naturally Occurring ◽  
Gross Lesions ◽  
Immunohistochemical Techniques

A Mycobacterium bovis-infected herd of captive wapiti (Cervus elaphus nelsoni) in Colorado was depopulated after lesions of bovine tuberculosis were confirmed in 8 of 10 tuberculin skin test reactors. Of the 43 animals > 1 year of age, 26 had gross lesions suggestive of tuberculosis, 24 had microscopic lesions of tuberculosis, and 23 had acid-fast bacilli associated with the lesions. Lungs and retropharyngeal lymph nodes were the most frequently affected sites. Most lesions grossly and microscopically resembled tuberculosis in cattle; however, some lesions resembled abscesses or ovine caseous lymphadenitis lesions. Special stains and immunohistochemical techniques labeled few to numerous mycobacteria associated with the lesions.

scholarly journals The Bovine Tuberculoid Granuloma

Pathogens ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 61
Author(s):  
Mitchell V. Palmer ◽  
Carly Kanipe ◽  
Paola M. Boggiatto
Keyword(s):  
Lymph Nodes ◽  
Disease Severity ◽  
Mycobacterium Bovis ◽  
Vaccine Efficacy ◽  
Bovine Tuberculosis ◽  
Regional Lymph Nodes ◽  
Cytokines And Chemokines ◽  
Successful Control ◽  
Bacterial Replication ◽  
Efficacy Studies

The bovine tuberculoid granuloma is the hallmark lesion of bovine tuberculosis (bTB) due to Mycobacterium bovis infection. The pathogenesis of bTB, and thereby the process of bovine tuberculoid granuloma development, involves the recruitment, activation, and maintenance of cells under the influence of antigen, cytokines and chemokines in affected lungs and regional lymph nodes. The granuloma is key to successful control of bTB by preventing pathogen dissemination through containment by cellular and fibrotic layers. Paradoxically, however, it may also provide a niche for bacterial replication. The morphologic and cellular characteristics of granulomas have been used to gauge disease severity in bTB pathogenesis and vaccine efficacy studies. As such, it is critical to understand the complex mechanisms behind granuloma initiation, development, and maintenance.

scholarly journals First approach to molecular epidemiology of bovine tuberculosis in Colombia

2016 ◽  
pp. 5222-5236 ◽  
Author(s):  
Jimena Jojoa-Jojoa ◽  
Maira Wintaco M ◽  
Francisco Osorio R ◽  
Gloria Puerto-Castro ◽  
Martha Guerrero-Guerrero
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Microbiological Analysis ◽  
Mycobacterium Species ◽  
Molecular Assays ◽  
Nasal Swabs ◽  
The World ◽  
Gross Lesions

ABSTRACT Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.RESUMEN Objetivo. Investigar la presencia de Mycobacterium bovis y otras especies de Mycobacterium en muestras de ganado bovino y búfalino en Colombia e iniciar la caracterización molecular de M. bovis en el país. Materiales y métodos. 492 muestras fueron recogidas de hatos identificados con presencia de animales infectados a través de la prueba de PPD, por el Grupo de Tuberculosis Bovina del Instituto Colombiano Agropecuario ICA en ocho departamentos de Colombia. Se incluyeron ganglios linfáticos de cabeza, tórax y abdomen, tejidos con lesiones macroscópicas de tuberculosis, hisopos nasales, leche, sangre y quesos frescos. Las muestras se sometieron a investigación de Mycobacterium bovis y otras micobacterias mediante el análisis microbiológico convencional y pruebas moleculares de PCR-IS6110 y spoligotyping. Resultados. En las muestras analizadas especialmente en los ganglios linfáticos, se evidenció Mycobacterium bovis con genotipos reportados y no reportados previamente en el mundo, así como M. tuberculosis en los departamentos de Antioquia, Cundinamarca, Magdalena y Boyacá. Conclusiones. En Colombia existen al menos 7 genotipos de M. bovis que se encuentran infectando y enfermando a bovinos y bufalinos de cuatro diferentes departamentos del país constituyéndose en seria amenaza para la salud pública.

scholarly journals Mycobacterium bovis: an extraordinary pathogen

2004 ◽  
Vol 25 (4) ◽  
pp. 15
Author(s):  
Debby Cousins
Keyword(s):  
International Trade ◽  
Human Health ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Developed Countries ◽  
Zoonotic Disease ◽  
Causative Organism ◽  
Crossing Over ◽  
Tuberculosis Complex ◽  
Evolutionary Spectrum

Mycobacterium bovis, originally considered to be the precursor of human tuberculosis by ?crossing over? when man first began domesticating animals, is now considered at the opposite end of the evolutionary spectrum. M. bovis belongs in the M. tuberculosis complex, a group of organisms that has, until relatively recently, contained the established pathogens M. tuberculosis, M. africanum, M. bovis (and M. bovis BCG) and M. microti. M. tuberculosis and M. africanum primarily affect humans and M. microti is the main pathogen of voles. M. bovis is the causative organism of bovine tuberculosis, a well known zoonotic disease that can impact on human health, international trade and production. In fact, because of these factors, most developed countries have spent large sums of money attempting to eradicate or control bovine tuberculosis.

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