scholarly journals Molecular Techniques for Identification of Species of the Mycobacterium tuberculosis Complex: The use of Multiplex PCR and an Adapted HPLC Method for Identification of Mycobacterium bovis and Diagnosis of Bovine Tuberculosis

10.5772/33876 ◽  
2012 ◽  
Author(s):  
Eduardo Eustquio de Souza Figueiredo ◽  
Carlos Adam Conte Jnior ◽  
Leone Vincius ◽  
Flvia Galindo Silvestre Silva ◽  
Rafael Silva ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Hplc Method ◽  
Molecular Techniques ◽  
Tuberculosis Complex ◽  
Identification Of Species

scholarly journals Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

2014 ◽  
Vol 45 (3) ◽  
pp. 841-843 ◽  
Author(s):  
F.L.E. Spositto ◽  
P.A.Z. Campanerut ◽  
L.D. Ghiraldi ◽  
C.Q.F. Leite ◽  
M.H. Hirata ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Mycobacterium Bovis ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex

scholarly journals Tuberculosis in Antelopes in a Zoo in Poland – Problem of Public Health

2015 ◽  
Vol 64 (4) ◽  
pp. 395-397 ◽  
Author(s):  
Monika Krajewska ◽  
Michał Załuski ◽  
Anna Zabost ◽  
Blanka Orłowska ◽  
Ewa Augustynowicz-Kopeć ◽  
...  
Keyword(s):  
Public Health ◽  
Infectious Disease ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Wild Animals ◽  
Tuberculosis Complex ◽  
Mycobacterium Caprae ◽  
In Captivity

Bovine tuberculosis is an infectious disease that occurs in many species of both domestic and wild animals, as well as those held in captivity. The etiological factor is the acid resistant bacillus (Mycobacterium bovis or Mycobacterium caprae), which is characterized by the major pathogenicity among mycobacteria belonging to the Mycobacterium tuberculosis complex. The material from 8 antelopes from the zoo, suspected for tuberculosis were examined, and M. bovis strains were isolated from 6 of them. The spoligotyping method showing spoligo pattern 676763777777600. In Poland, this spoligotype has not been observed so far.

PCR Detection of Mycobacterium bovis DNA in Cattle Bloods

2013 ◽  
Vol 319 ◽  
pp. 169-173
Author(s):  
Chun Fang Wang ◽  
Yun Hang Gao ◽  
Xiu Yun Jiang ◽  
Jian Wang ◽  
Gong Mei Li ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Mycobacterium Bovis ◽  
Whole Blood ◽  
Mycobacterium Tuberculosis Complex ◽  
Pcr Detection ◽  
Biological Detection ◽  
Tuberculosis Complex ◽  
Blood Samples ◽  
Whole Blood Samples

For molecular biological detection of Mycobacterium tuberculosis, PCR methods with primers targeting different regions specific for mycobacterium tuberculosis complex are used worldwide. In this study,256 whole blood samples were detected using multiplex PCR.2 of all samples were found to be PCR positive. Homogenous comparison of Mycobacterium bovis specific genes with Mycobacterium bovis AF2122/97 all reached up to 99%.

scholarly journals Development and application of affordable SNP typing approaches to genotype Mycobacterium tuberculosis complex strains in low and high burden countries

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Irving Cancino-Muñoz ◽  
Ana Gil-Brusola ◽  
Manuela Torres-Puente ◽  
Carla Mariner-Llicer ◽  
John Dogba ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Low Income ◽  
Sanger Sequencing ◽  
High Resolution Melting ◽  
Mycobacterium Tuberculosis Complex ◽  
Molecular Techniques ◽  
Low Income Countries ◽  
Tuberculosis Complex ◽  
Typing Methods

Abstract The Mycobacterium tuberculosis complex (MTBC) comprises the species that causes tuberculosis (TB) which affects 10 million people every year. A robust classification of species, lineages, and sub-lineages is important to explore associations with drug resistance, epidemiological patterns or clinical outcomes. We present a rapid and easy-to-follow methodology to classify clinical TB samples into the main MTBC clades. Approaches are based on the identification of lineage and sub-lineage diagnostic SNP using a real-time PCR high resolution melting assay and classic Sanger sequencing from low-concentrated, low quality DNA. Thus, suitable for implementation in middle and low-income countries. Once we validated our molecular procedures, we characterized a total of 491 biological samples from human and cattle hosts, representing countries with different TB burden. Overall, we managed to genotype ~95% of all samples despite coming from unpurified and low-concentrated DNA. Our approach also allowed us to detect zoonotic cases in eight human samples from Nigeria. To conclude, the molecular techniques we have developed, are accurate, discriminative and reproducible. Furthermore, it costs less than other classic typing methods, resulting in an affordable alternative method in TB laboratories.

Sign in / Sign up

Export Citation Format

Share Document