scholarly journals ДІАГНОСТИКА ТА ЗАХОДИ БОРОТЬБИ З ПАСТЕРЕЛЬОЗОМ ГУСЕЙ В ПРИВАТНОМУ ЗООПАРКУ ПОЛТАВСЬКОЇ ОБЛАСТІ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 152-157
Author(s):  
O.O. Peredera ◽  
I.V. Lavrinenko ◽  
I.A. Zhernosik
Keyword(s):  
High Efficiency ◽  
Clinical Signs ◽  
High Sensitivity ◽  
Petri Dish ◽  
Growth Zone ◽  
Diffusion Method ◽  
Growth Delay ◽  
Stunted Growth ◽  
Therapeutic Measures ◽  
Moderately Resistant

The results of the study geese pasteurellosis outbreaks in private zoo in the Poltava region. Diagnosis for pasteurellosis set comprehensively, taking into account epizootological features, clinical signs and pathological changes.The diagnosis was carried out comprehensively, taking into account the characteristics of epizootic, clinical signs and postmortem autopsy results. In determining the diagnosis were the results of microscopic and bacteriological research. To study the morphology of microorganisms strokes, the selected cultures were stained with alcoholic–aqueous methylene blue. We studied the sensitivity of selected microorganisms to certain antibiotics. After 24 hours of incubation in nutrient medium grew rounded, smooth, «wax» colonies of microorganisms.In smears of colonies that grew were found to IPA Pasteurella – typical bipolar coli. After obtaining a pure culture was carried determine the sensitivity of the selected pathogen to various antibacterial agents. For this we used disco–diffusion method. After the incubation, the Petri dish looked and measured in millimeters zone delay line growth of microorganisms.According to the results obtained, isolated Pasteurella culture revealed high sensitivity to tilozyn. This antibacterial agent caused stunted growth zone microflora 14–21 mm; High sensitivity also highlighted the culture was determined to enrofloxacin (zone of stunted growth – 16–24 mm) and gentamicin (zone of stunted growth – 18–24 mm) Moderately resistant culture was to kanamycin and chloramphenicol (zone growth retardation – 8–14 mm in accordance). To the rest of the drugs was highlighted microflora resistant, zone microflora growth delay ranged from 0 to 4 mm.The drug Enroksil 10% solution, based on enrofloxacin used orally in drinking water at the rate of 5 ml per 10 liters of water for three days. During treatment birds received only water containing the drug. The high sensitivity of microflora selected a number of antibacterial drugs and high efficiency of therapeutic measures can be explained by timely treatment zoo owner for help with diagnosis.

scholarly journals Визначення чутливості виробничого штаму Bacillus anthracis UA–07 до антибіотиків

2017 ◽  
Vol 19 (73) ◽  
pp. 84-88
Author(s):  
I. Rublenko ◽  
V. Skripnik
Keyword(s):  
Bacillus Anthracis ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Growth Delay ◽  
Genetic Changes ◽  
Stunted Growth ◽  
Highly Sensitive ◽  
And Control ◽  
Zone Growth

Long-term use of antibiotics leads to antibiotic resistance in pathogens. In addition, there is a possibility of permanent genetic changes in strains due to environmental influences. Therefore there is a need for periodic study vaccine strains for sensitivity to antibiotics in order to study and control of their passport characteristics. The aim of our research was necessary to determine the sensitivity of the strain of Bacillus anthracis UA-07 to antibiotics. The results of studies of sensitivity of strain Bacillus anthracis UA–07 to 50 antibiotics were determined by the size of the diameter of the zones of growth retardation. Zone growth delay of 15 mm was considered a sign of a weak antibiotic sensitivity, zone 15–24 mm – a sign of sensitivity and area of more than 24 mm - a sign of the high sensitivity of the strain to this antibiotic. Lack of stunted growth areas points to the insensitivity of the strain to this antibiotic. The research results show that the strain of Bacillus anthracis UA–07 is highly sensitive to ofloxacin (Of5), ampicillin / sulbactam (A/S10/10), linezolid (LZ30), amoxicillin (AMX30), norfloxacin (NX10), cefalotin (CEP30), tetracycline (TE30). Not affect the development of the studied microorganism antibiotics, cefepime (SPM30), nystatin (NS100U) tsefuroksyn (CXM30) tsefeksym (CFM5), sulfadiazine (SZ300), metronidazole (MT4), cefazolin (Cz30).

Trends in Diagnosis for Active Tuberculosis Using Nanomaterials

2019 ◽  
Vol 26 (11) ◽  
pp. 1946-1959 ◽  
Author(s):  
Le Minh Tu Phan ◽  
Lemma Teshome Tufa ◽  
Hwa-Jung Kim ◽  
Jaebeom Lee ◽  
Tae Jung Park
Keyword(s):  
Sensitivity And Specificity ◽  
High Efficiency ◽  
Point Of Care ◽  
High Sensitivity ◽  
Signs And Symptoms ◽  
Diagnostic Methods ◽  
Advantages And Disadvantages ◽  
Treatment And Prevention ◽  
Clinical Tools ◽  
Diagnostic Applications

Background:Tuberculosis (TB), one of the leading causes of death worldwide, is difficult to diagnose based only on signs and symptoms. Methods for TB detection are continuously being researched to design novel effective clinical tools for the diagnosis of TB.Objective:This article reviews the methods to diagnose TB at the latent and active stages and to recognize prospective TB diagnostic methods based on nanomaterials.Methods:The current methods for TB diagnosis were reviewed by evaluating their advantages and disadvantages. Furthermore, the trends in TB detection using nanomaterials were discussed regarding their performance capacity for clinical diagnostic applications.Results:Current methods such as microscopy, culture, and tuberculin skin test are still being employed to diagnose TB, however, a highly sensitive point of care tool without false results is still needed. The utilization of nanomaterials to detect the specific TB biomarkers with high sensitivity and specificity can provide a possible strategy to rapidly diagnose TB. Although it is challenging for nanodiagnostic platforms to be assessed in clinical trials, active TB diagnosis using nanomaterials is highly expected to achieve clinical significance for regular application. In addition, aspects and future directions in developing the high-efficiency tools to diagnose active TB using advanced nanomaterials are expounded.Conclusion:This review suggests that nanomaterials have high potential as rapid, costeffective tools to enhance the diagnostic sensitivity and specificity for the accurate diagnosis, treatment, and prevention of TB. Hence, portable nanobiosensors can be alternative effective tests to be exploited globally after clinical trial execution.

scholarly journals Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 667
Author(s):  
Pavel Rudenko ◽  
Yuriy Vatnikov ◽  
Nadezhda Sachivkina ◽  
Andrei Rudenko ◽  
Evgeny Kulikov ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
High Efficiency ◽  
Biological Marker ◽  
High Sensitivity ◽  
Antagonistic Activity ◽  
Adhesive Properties ◽  
Surgical Infection ◽  
Surgical Infections ◽  
Causative Agents ◽  
Inflammatory Processes

Despite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent–inflammatory processes remains one of the most complex and urgent problems in veterinary practice. The article presents the results of the isolation of indigenous microbiota from various biotopes of healthy cats, as well as the study of their biological marker properties for the selection of the most optimal strains in probiotic medicines for the control of surgical infections. It was demonstrated that isolated cultures of bifidobacteria and lactobacilli, which we isolated, revealed high sensitivity to antibiotics of the β-lactam group (excepting L. acidophilus No. 24, L. plantarum “Victoria” No. 22, L. rhamnosus No. 5, L. rhamnosus No. 20, and L. rhamnosus No. 26, which showed a significant variability in sensitivity to antibacterial drugs of this group, indicating the great potential of these microorganisms) and resistance to aminoglycosides, lincosamides, and fluoroquinolones (with the exception of gatifloxacin, which showed high efficiency in relation to all lactic acid microorganisms). The adhesive properties of the isolated lactobacteria and bifidobacteria were variable, even within the same species. It was found that the B. adolescentis No. 23 strain of the Bifidobacterium genus, as well as the L. plantarum No. 8, L. plantarum “Victoria” No. 22, L. rhamnosus No. 6, L. rhamnosus No. 26, L. acidophilus No. 12, and L. acidophilus No. 24 strains of the Lactobacillus genus had the highest adhesive activity. Thus, when conducting a detailed analysis of the biological marker properties of candidate cultures (determining their sensitivity to antimicrobial agents, studying the adhesive properties, and antagonistic activity in relation to causative agents of surgical infection in cats), it was found that the most promising are L. plantarum “Victoria” No. 22, L. rhamnosus No. 26, and L. acidophilus No. 24.

Low-frequency multiplex CARS microscopy with a high-repetition near-infrared supercontinuum laser

2021 ◽  
Author(s):  
Yusuke Arashida ◽  
Atsushi Taninaka ◽  
Takayuki Ochiai ◽  
Hiroyuki Mogi ◽  
Shoji YOSHIDA ◽  
...  
Keyword(s):  
Near Infrared ◽  
High Efficiency ◽  
Signal To Noise Ratio ◽  
Low Frequency ◽  
High Sensitivity ◽  
High Repetition ◽  
Spectrum Shaping ◽  
Crystal Phase Transition ◽  
Wavenumber Region ◽  
Anti Stokes

Abstract We have developed a multiplex Coherent anti-Stokes Raman scattering (CARS) microscope effective for low-wavenumber measurement by combining a high-repetition supercontinuum light source of 1064 nm and an infrared high-sensitivity InGaAs diode array. This system could observe the low-wavenumber region down to 55 cm-1 with high sensitivity. In addition, using spectrum shaping and spectrum modulation techniques, we simultaneously realized a wide bandwidth (<1800 cm-1), high wavenumber resolution (9 cm-1), high efficiency, and increasing signal to noise ratio by reducing the effect of the background shape in low-wavenumber region. Spatial variation of a sulfur crystal phase transition with metastable states was visualized.

Markers of the early stage of rheumatoid arthritis

2016 ◽  
Vol 51 (4) ◽  
pp. 305-314
Author(s):  
Beata Polińska ◽  
Joanna Matowicka-Karna ◽  
Halina Kemona
Keyword(s):  
Rheumatoid Arthritis ◽  
Human Population ◽  
Early Stage ◽  
Clinical Signs ◽  
High Sensitivity ◽  
Unknown Etiology ◽  
Serological Tests ◽  
Citrullinated Peptide ◽  
Peptide Antibodies

Rheumatoid arthritis (RA) is a chronic, autoimmune connective tissue disease of unknown etiology. RA affects about 1% of the human population, women suffer three times more often than men, with the peak incidence between the age of 40 to 50. The up-to-date criteria from 2010 for the diagnosis of RA include: occurrence and duration of clinical signs, indicators of inflammation and serological tests. Neopterin, a protein released by macrophages, is a sensitive indicator of inflammation and the severity of RA. Regarding the serological tests, anti-cyclic citrullinated peptide antibodies represent a well-known marker with the specificity for RA of about 98%. The antibodies may be present in the serum of patients even a few years before the first clinical signs of the disease, heralding erosive changes in the joints and more severe course of RA. The literature also contains reports about autoantibodies anti-CarP and anti-Sa/ anti-MCV, which may occur in people with pain and swelling of joints and precede full-blown development of RA as well as reflect disease activity. Serological diagnosis of RA may be supported by some genetic tests based on PCR for detecting mutations e.g. C1858T in the PNPN22 gene. In turn, the quantitative analysis of different classes of miRNAs seems justified in order to better classify patients showing symptoms of RA. Further studies are needed that take into account the role of different markers in the development of RA, and confirm the high sensitivity and specificity of these markers in the diagnosis of the disease.

scholarly journals Proposal of an Algorithm to Early Detect Attenuated Type I Mucopolysaccharidosis (MPS Ia) among Children with Growth Abnormalities

Medicina ◽  
2022 ◽  
Vol 58 (1) ◽  
pp. 97
Author(s):  
Federico Baronio ◽  
Stefano Zucchini ◽  
Francesco Zulian ◽  
Mariacarolina Salerno ◽  
Rossella Parini ◽  
...  
Keyword(s):  
Short Stature ◽  
Metabolic Disorders ◽  
Clinical Presentation ◽  
Diagnostic Delay ◽  
Clinical Signs ◽  
Clinical Approach ◽  
Type I ◽  
Mucopolysaccharidosis Type I ◽  
Stunted Growth ◽  
Growth Abnormalities

Background and Objectives: Diagnostic delay is common in attenuated Mucopolysaccharidosis Type I (MPS Ia) due to the rarity of the disease and the variability of clinical presentation. Short stature and impaired growth velocity are frequent findings in MPS Ia, but they rarely raise suspicion as paediatric endocrinologists are generally poorly trained to detect earlier and milder clinical signs of this condition. Materials and Methods: Following a consensus-based methodology, a multidisciplinary panel including paediatric endocrinologists, paediatricians with expertise in metabolic disorders, radiologists, and rheumatologists shared their experience on a possible clinical approach to the diagnosis of MPS Ia in children with short stature or stunted growth. Results: The result was the formation of an algorithm that illustrates how to raise the suspicion of MPS Ia in a patient older than 5 years with short stature and suggestive clinical signs. Conclusion: The proposed algorithm may represent a useful tool to improve the awareness of paediatric endocrinologists and reduce the diagnostic delay for patients with MPS Ia.

scholarly journals Antimicrobial resistance in bacteria isolated from pigs with respiratory clinical signs in Brazil

2020 ◽  
Vol 57 (1) ◽  
pp. e160956
Author(s):  
Maysa Serpa ◽  
Juliana Amália Fonte Bôa do Nascimento ◽  
Mirian Fátima Alves ◽  
Maria Isabel Maldonado Coelho Guedes ◽  
Adrienny Trindade Reis ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Clinical Signs ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Great Level ◽  
Respiratory Pathogens ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Pig Farms

Antimicrobial resistance is a current and important issue to public health, and it is usually associated with the indiscriminate use of antimicrobials in animal production. This study aimed to evaluate the antimicrobial susceptibility profile in bacterial isolates from pigs with clinical respiratory signs in Brazil. One hundred sixty bacterial strains isolated from pigs from 51 pig farms in Brazil were studied. In vitro disk-diffusion method was employed using 14 antimicrobial agents: amoxicillin, penicillin, ceftiofur, ciprofloxacin, enrofloxacin, chlortetracycline, doxycycline, oxytetracycline, tetracycline, erythromycin, tilmicosin, florfenicol, lincomycin, and sulfadiazine/trimethoprim. The majority of isolates were resistant to at least one antimicrobial agent (98.75%; 158/160), while 31.25% (50/160) of the strains were multidrug resistant. Streptococcus suis and Bordetella bronchiseptica were the pathogens that showed higher resistance levels. Haemophilus parasuis showed high resistance levels to sulfadiazine/trimethoprim (9/18=50%). We observed that isolates from the midwestern and southern regions exhibited four times greater chance of being multidrug resistant than the isolates from the southeastern region studied. Overall, the results of the present study showed a great level of resistance to lincomycin, erythromycin, sulfadiazine/trimethoprim, and tetracycline among bacterial respiratory pathogens isolated from pigs in Brazil. The high levels of antimicrobial resistance in swine respiratory bacterial pathogens highlight the need for the proper use of antimicrobials in Brazilian pig farms.

Aerothermal Simulations Comparisons of a Shaped-Hole Film Cooling Flow

2019 ◽  
Author(s):  
Matthieu Simon ◽  
Sébastien Gautier ◽  
Emmanuel Vanoli ◽  
Pierre Auzillon
Keyword(s):  
Numerical Simulations ◽  
Film Cooling ◽  
Test Bench ◽  
High Efficiency ◽  
Density Ratio ◽  
High Sensitivity ◽  
Good Prediction ◽  
Ansys Fluent ◽  
Free Stream Turbulence ◽  
Complete Test

Abstract Film Cooling is a crucial technology for engine manufacturer to develop high-efficiency gas turbine engines by raising turbine entry temperature. A lot of cooling holes geometries have been studied in the past few years in tests, as well as numerical simulations. Shaped holes are nowadays a standard geometry for protecting the blades, given the performance improvement compared to cylindrical holes. Numerical correlation with physical tests is challenging due to the high sensitivity to thermal mixing and adequate boundary condition predictions. This paper is devoted to numerical simulation comparisons of the 777 shaped holes configuration of Pennsylvania State University, for an incompressible flow with a density ratio of 1.5, a blowing ratio of 1.5 and a free stream turbulence intensity of 0.5%. Two different simulations have been chosen: a state-of-the-art RANS simulation with k-e Realizable model computed with ANSYS Fluent and a high fidelity solver Lattice-Boltzmann Method computed with Simulia PowerFLOW. In order to improve the accuracy of numerical simulations against test results, this article deals with an aerothermal model of the complete test bench. This additional modeling allows to strongly improve thermal prediction and to understand initial discrepancies related to test bench environment. Results show that k-ε Realizable simulation provides a good prediction of average effectiveness, but local differences appear due to inherent RANS modeling limitations. On the other hand, LBM simulation provides excellent results for both aerodynamic and thermal quantities: tests results are very well reproduced.

Sign in / Sign up

Export Citation Format

Share Document