scholarly journals Імунологічні зміни у вагітних із дисбіотичними порушеннями

2012 ◽  
Vol 3 (1) ◽  
pp. 85-90
Author(s):  
Y. Y. Nikulina ◽  
I. E. Sokolova ◽  
A. I. Vinnikov
Keyword(s):  
Toxoplasma Gondii ◽  
Pregnant Women ◽  
Immune Complexes ◽  
Trichomonas Vaginalis ◽  
Biological Properties ◽  
Gardnerella Vaginalis ◽  
Vaginal Smears ◽  
Cell Parameters ◽  
Clinical Hospital ◽  
Candida Sp

The biological properties of the vaginosis pathogens isolated from 255 pregnant patients of the Pregnancy Pathology Department of the Mechnikov Dnipropetrovsk Region Clinical Hospital were studied. It was found that all investigated microflora was represented by the Candida yeasts, Trichomonas vaginalis, Toxoplasma gondii, Chlamidia trachomatis, Gardnerella vaginalis and Leptotrix vaginalis. The yeasts of genus Candida were the most common isolated pathogens of the vaginosis in pregnant women. The presence of Candida sp., Trichomonas vaginalis, Chlamidia trachomatis and Toxoplasma gondii in the vaginal smears taken from pregnant women was accompanied by changes of the immunity cell parameters and concentration of circulating immune complexes. 

TRICHOMONAS VAGINALIS E ANORMALIDES CITOPATÓLOGICAS NOS EXAMES CITOPATOLÓGICOS REALIZADOS EM UM LABORATÓRIO CLÍNICO DA PONTIFÍCIA UNIVERSIDADE CATÓLICA DE GOIÁS - GO: ESTUDO DE PREVALÊNCIA

2020 ◽  
Vol 47 (1) ◽  
pp. 6104
Author(s):  
Andrea Alves Ribeiro ◽  
Sara Rosa Peixoto ◽  
Gabriel Henrique Martins de Almeida Araújo ◽  
Carolina Tonello dos Santos Pelá Alcanfor
Keyword(s):  
Herpes Simplex ◽  
Chlamydia Trachomatis ◽  
Odds Ratio ◽  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Sp

O Trichomonas vaginalis é um protozoário que causa uma das principais infecções sexualmente transmissíveis. O objetivo foi estimar a prevalência de Trichomonas vaginalis em um Laboratório escola de Goiânia-GO. É um estudo retrospectivo utilizando as fichas de requisição dos exames citopatológicos realizados no Laboratório Clínico da Pontifícia Universidade Católica de Goiás (LC-PUCGO) entre janeiro de 2013 a dezembro de 2015. As variáveis foram categorizadas no programa Excel 2013 e tabulados no programa Epi Info™ versão 7, para avaliação da associação entre as variáveis foi realizado o cálculo do Odds Ratio (OR), considerando um intervalo de confiança (IC) de 95% e o valor de significância p< 0,05. Do total dos 4558 exames citopatológicos satisfatórios foi encontrada uma prevalência de Lactobacillus sp., de 46,97% (2141/4558) seguida por bacilos 24,62% (1122/4558), bacilos e cocos de 21,68% (988/4558), cocos de 3,86%(176/4558) e bacilos e lactobacilos de 2,87% (131/4558). Os agentes patogênicos encontrados foram Gardnerella vaginalis 79,6% (1141/1433), Candida sp. 17,7% (253/1433), Trichomonas vaginalis de 2,2% (32/1433), Herpes simplex de 0,4% (6/1433) e Chlamydia trachomatis de 0,1% (1/1433). Dentre os 4558 exames citopatológicos, 14,39% (656/4558) apresentaram anormalidades citológicas. As anormalidades encontradas foram ASC-US 2,57% (117/656), LSIL 1,78 % (81/656), ASC-H 3,52 % (160/656), HSIL 1,08% (48/656), AGC NEO 0,22% (10/656), Carcinoma invasor 0,02 % (1/656). Houve uma associação estatisticamente significante com a presença de Trichomonas vaginalis e o padrão anaeróbio OR 44,89 (IC 95 %: 10.5-271,8 p < 0,000). A detecção da manifestação por Trichomonas vaginalis é bastante sensível por meio do exame citopatológico. A presença de Trichomonas vaginalis, em um percentual relativamente baixo, indica a necessidade de identificação dos fatores de risco e aconselhamento em relação à proteção, transmissão e tratamento dessa infecção.

IDENTIFICATION OF CANDIDA SP., GARDNERELLA VAGINALIS AND TRICHOMONAS VAGINALIS BY MEANS OF THE AFFIRM(R) VPIII TEST

Mycoses ◽  
2002 ◽  
Vol 45 (S2) ◽  
pp. 24-24
Author(s):  
U.-C. Hipler ◽  
C. Greif ◽  
U. Metzner ◽  
C. Hipler ◽  
P. Elsner
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Sp

scholarly journals Papillomavirus in cervicovaginal smears of women infected with human immunodefficiency virus

1995 ◽  
Vol 113 (6) ◽  
pp. 1009-1011 ◽  
Author(s):  
Edenilson Eduardo Galore ◽  
Maria José Cavaliere ◽  
Neuza Kasumi Shirata ◽  
Maria de Fátima Araújo
Keyword(s):  
Trichomonas Vaginalis ◽  
Intraepithelial Neoplasia ◽  
Gardnerella Vaginalis ◽  
Herpes Simplex Virus 1 ◽  
Candida Sp ◽  
Cytopathic Effects ◽  
Immunological Factors ◽  
Simplex Virus ◽  
Invasive Carcinomas ◽  
Hiv Seropositive

It has been described that women infected with the human immunodeffciency virus (HIV) present more frequent cytological abnormalities in cervicovaginal smears, generally related to infection by human papillomavirus (HPV). The present work is a study of cervicovaginal smears of 147 HIV-seropositive women submitted to routine gynecological examinations. The smears were stained by the Papanicolaou method. Cytopathic effects of HPV were found in 38 (25.8%) cases. Nuclear atypias of cervical intraepithelial neoplasia (CIN) were evident in 36 (24.5%) of these cases: 27 (18.4%), CIN I; 6 (4.0%), CIN II and 3 (2.0%) CIN III. Also 2 (1.4%) invasive carcinomas and one (0.7%) endocervical dysplasia were found. Other agents observed were: Candida sp, 19 (12.9%) cases, Gardnerella vaginalis, 19 (12.9%), Trichomonas vaginalis, 13 (8.4%), Chlamydia trachomatis 5 (3.4%), Mobiluncus sp 2 (1.4%) and Herpes simplex virus 1 (0.7%). This study emphasizes the high frequency of HPV/CIN cervicovaginal abnormalities in HIV-seropositive in our population. It is possible that immunological factors and sexual promiscuity are involved in this phenomenon.

scholarly journals Análise dos resultados do último laudo citopatológico de pacientes com Papanicolaou em atraso

2020 ◽  
Vol 11 (3) ◽  
Author(s):  
Nathanael De Souza Maciel ◽  
Francisco Jardsom Moura Luzia ◽  
Diego Da Silva Ferreira ◽  
Maria Charlianne de Lima Pereira Silva ◽  
Davide Carlos Joaquim ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Pap Smear ◽  
Gardnerella Vaginalis ◽  
Cervical Lesions ◽  
Papanicolaou Test ◽  
Pap Tests ◽  
Sexually Transmitted ◽  
Candida Sp ◽  
Infecciones De Transmisión Sexual

Objetivo: Analisar os resultados do último laudo citopatológico de pacientes com o exame Papanicolaou em atraso. Metodologia: Estudo documental, retrospectivo, com abordagem quantitativa, realizado no município de Redenção–CE. Foram explorados 660 prontuários de mulheres com idade entre 25 e 64 anos. Para análise foram utilizadas medidas de tendência central e frequências absolutas e relativas. Resultados: O número de mulheres que nunca haviam realizado o exame Papanicolaou foi de 44,7% e as que haviam realizado, mas estavam em atraso, foi de 55,3%. A presença de células metaplásicas foi menos frequente (38,6%). Entre agentes infecciosos, houve prevalência dos resultados sugestivos de Gardnerella vaginalis e Mobiluncus (14,9%), Cândida sp (3,9%) e Trichomonas vaginalis (1,1%).  Os laudos indicaram presença de alterações celulares pré-malignas e malignas. Conclusão: Esta análise poderá motivar a implementação da busca de pacientes com exame atrasado, especialmente das que apresentam alguma infecção sexualmente transmissível ou alteração celular pré-maligna ou maligna.Descritores: Neoplasias do Colo do Útero; Teste de Papanicolaou; Programas de Rastreamento; Atenção Primária à Saúde, Enfermagem.ANALYSIS OF THE RESULTS OF THE LAST CYTOPATHOLOGICAL EXAM OF PATIENTS WITH DELAYED PAP SMEARObjective: To analyze the results of the last cytopathological report of patients with delayed Pap smear. Methodology: Documentary, retrospective study with quantitative approach, conducted in the city of Redenção-CE, from April to September 2018. Were explored 660 medical records of women aged 25 to 64 years. For analysis, measures of central tendency and absolute and relative frequencies were used. Results: The number of women who had never had the Pap smear was 44,7%, and those who had been submitted but were delayed were 55,3%. The presence of metaplastic cells was less frequent (38,6%). Among infectious agents, there was a prevalence of suggestive results of Gardnerella vaginalis and Mobiluncus (14,9%), Candida sp (3,9%) and Trichomonas vaginalis (1,1%). The reports indicated the presence of premalignant and malignant cellular alterations. Conclusion: There are a large number of women with delayed Pap tests and a deficiency in the quality of the tests, indicated by the absence of metaplastic tissue. This should motivate the active search of patients to interrupt the chain of transmission of Sexually Transmitted Infections and to treat cervical lesions early, reducing the chances of progression.KEYWORDS: Uterine Cervical Neoplasms, Papanicolaou Test, Mass Screening, Primary Health Care, Nursing. ANÁLISIS DE LOS RESULTADOS DEL ÚLTIMO EXAMEN CITOPATOLÓGICO DE PACIENTES CON PAPANICOLAOU EN RETRASOObjetivo: Analizar resultados del último examen citopatológico de pacientes con Papanicolaou en retraso. Metodología: Estudio documental, retrospectivo, con enfoque cuantitativo, realizado en la ciudad de Redenção-CE, de abril a septiembre de 2018. Fueron explorados 660 prontuarios de mujeres de 25 a 64 años. Para el análisis, se utilizaron medidas de tendencia central, frecuencias absolutas y relativas. Resultados: El número de mujeres que nunca se habían hecho la prueba de Papanicolaou fue del 44,7%, y las que se sometieron, pero estában en retraso, fueron 55,3%. La presencia de células metaplásicas fue menos frecuente (38,6%). Entre los agentes infecciosos, hubo una prevalencia de resultados sugestivos de Gardnerella vaginalis y Mobiluncus (14,9%), Candida sp (3,9%) y Trichomonas vaginalis (1,1%). Los informes indicaron presencia de alteraciones celulares premalignas y malignas. Conclusión: Hoy una gran cantidad de mujeres con pruebas de Papanicolaou retrasadas y una deficiencia en la calidad de los exámenes, indicada por la ausencia de tejido metaplásico. Esto debería motivar la búsqueda activa de pacientes para interrumpir la cadena de transmisión de las infecciones de transmisión sexual y para tratar las lesiones cervicales temprano, reduciendo las posibilidades de progresión.PALABRAS-CLAVE: Neoplasias del Cuello Uterino, Prueba de Papanicolaou, Tamizaje Masivo, Atención Primaria de Salud, Enfermería.

scholarly journals ANÁLISE DOS LAUDOS DE PAPANICOLAOU REALIZADOS EM UMA UNIDADE BÁSICA DE SAÚDE

2012 ◽  
Vol 17 (4) ◽  
Author(s):  
Ana Paula Vieira Bringel ◽  
Marina Pessoa de Farias Rodrigues ◽  
Eglídia Carla Figueirêdo Vidal
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Sp

Estudo descritivo teve por objetivo caracterizar os aspectos microbiológicos e epiteliais presentes nos exames preventivos realizados em uma unidade básica de saúde de Juazeiro do Norte - Ceará. Os dados foram coletados de julho a novembro de 2011, mediante a análise de 782 resultados de Papanicolaou realizados entre março de 2008 e outubro de 2011 e que constavam no livro de registros de preventivos da unidade. Os dados foram analisados com auxílio do programa estatístico SPSS 16.0. Os resultados evidenciaram maior procura de mulheres entre 25 a 34 anos (28,4%) para a realização do exame; no esfregaço houve prevalência de epitélio escamoso (80,9%), Cocos (49,2%) e agentes infecciosos como Gardnerella vaginalis (65%), Cândida sp (23,7%) e Trichomonas vaginalis (5,6%). O profissional de saúde precisa identificar os fatores de risco associados às afecções, possibilitando prevenção do câncer do colo uterino e controle das Doenças Sexualmente Transmissíveis.

scholarly journals Frequency of Trichomonas vaginalis, Candida sp and Gardnerella vaginalis in cervical-vaginal smears in four different decades

2001 ◽  
Vol 119 (6) ◽  
pp. 200-205 ◽  
Author(s):  
Sheila Jorge Adad ◽  
Rodrigo Vaz de Lima ◽  
Zahir Tannous Elias Sawan ◽  
Maria Letícia Gobo Silva ◽  
Maria Azniv Hazarabedian de Souza ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Significance Level ◽  
Tertiary Referral Center ◽  
Candida Sp ◽  
Gynecology And Obstetrics ◽  
Chi Squared ◽  
Causative Agents ◽  
Vaginal Cytology ◽  
De Medicina

CONTEXT: Vaginitis is one of the principal motives that lead women to seek out an obstetrician or gynecologist. Bacterial vaginosis, candidiasis and trichomoniasis are responsible for 90% of the cases of infectious vaginitis. OBJECTIVE: To verify the frequency of the three main causative agents of vaginitis, Trichomonas vaginalis, Candida sp and Gardnerella vaginalis, in four different decades (1960's, 1970's, 1980's and 1990's). DESIGN: Retrospective. PLACE: A tertiary referral center. PARTICIPANTS: Patients attended to as gynecology and obstetrics outpatients at the Faculdade de Medicina do Triângulo Mineiro during the years 1968, 1978, 1988, 1998, taken as samples of each decade. MAIN MEASUREMENTS: Diagnoses of infection by Trichomonas vaginalis, Candida sp and Gardnerella vaginalis were gathered from 20,356 cervical-vaginal cytology tests on patients attended to as gynecology outpatients at Faculdade de Medicina do Triângulo Mineiro during the years 1968, 1978, 1988, 1998, representing the four decades. The results were grouped according to the age group of the patients: under 20, between 20 and 29, between 30 and 39, between 40 and 49, and 50 or over. Statistical analysis was done via the chi-squared (Mantel-Haentzel) test with a significance level of 5%. RESULTS: In 1968 infections by Trichomonas vaginalis and Candida sp were diagnosed in 10% and 0.5% of the cytology tests and in 1978, 5.1% and 17.3%, respectively (P < 0.0001). Infection by Gardnerella vaginalis could only be evaluated in the latter two decades. In 1988, 19.8% of the women had positive tests for Gardnerella vaginalis, which was the most frequent agent in that year, diminishing in the subsequent decade to 15.9% (P < 0.0001). Candidiasis was the most frequent infection in 1998, detected in 22.5% of the tests (P < 0.0001). In a general manner, all the infections were most frequent among younger patients, especially those aged under 20, in all decades, whereas infections were least frequent among patients aged 50 or over (P < 0.05). CONCLUSION: There was a reduction in the frequency of cervical-vaginal infection by Trichomonas vaginalis and an increase in the frequency of Candida sp over the four decades studied. All the infections were most frequent in patients aged under 20 years.

scholarly journals Pesquisa de agentes infecciosos em exames citopatológicos de mulheres atendidas em uma unidade docente assistencial (UDA)

2019 ◽  
Vol 4 (3) ◽  
pp. 790-799
Author(s):  
Ayane Kelly Gomes dos Santos ◽  
Fernanda Barbosa da Silva ◽  
Evilma Nunes de Araújo ◽  
Samara Almeida de Souza Griz ◽  
Valéria Cristina de Melo Lopes ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Sp

As Doenças Sexualmente Transmissíveis (DSTs) predominam um importante problema de saúde pública mundial, sendo o diagnóstico rápido, mediante técnicas precisas, importante para que diminua a transmissão e consequências causadas pelas DSTs1. São três os principais tipos de vaginites infecciosas: vaginose bacteriana, candidíase e tricomoníase, sendo estes processos inflamatórios os que mais acometem os órgãos genitais femininos5. A avaliação foi realizada por meio de um estudo descritivo retrospectivo em 1240 laudos citológicos, coletados na Unidade Docente Assistencial (UDA) no Município de Maceió/AL no período de 2008 a 2012. Como critério de exclusão, pacientes que: não fazer o uso de cremes vaginal 24 horas antes do exame, não estar fazendo o uso de antibióticos, não estar no período menstrual, não ter relação sexual 48 horas antes do exame, como critério de participação do estudo. Os resultados, em relação à presença ou ausência dos agentes microbiológicos, foram distribuídos por faixa etária. Os seguintes intervalos foram analisados: 15 a 34 anos, 35 a 49 anos e de 50 a 65 anos. Verificou-se através das análises retrospectiva que (133/1240) laudos apresentaram agentes infecciosos. Destes 62,41% (83/133) foram positivos para Gardnerella vaginalis, 32,33% (43/133) para Candida sp, e 5,26% (7/133) para Trichomonas vaginalis.

scholarly journals Incidence of Gardnerella vaginalis, Candida sp and human papilloma virus in cytological smears

2000 ◽  
Vol 118 (4) ◽  
pp. 105-108 ◽  
Author(s):  
Eddie Fernando Candido Murta ◽  
Maria Azniv Hazarabedian de Souza ◽  
Eduardo Araújo Júnior ◽  
Sheila Jorge Adad
Keyword(s):  
Human Papilloma Virus ◽  
Trichomonas Vaginalis ◽  
Hpv Infection ◽  
Gardnerella Vaginalis ◽  
Control Group ◽  
Cytological Evidence ◽  
Papilloma Virus ◽  
Tertiary Referral Center ◽  
Candida Sp ◽  
Cytological Smear

CONTEXT: In spite of the wide-ranging literature on the microbiology of normal and abnormal flora of the vagina, there are few studies on the relationship between human papilloma virus (HPV) and other vaginal microorganisms. OBJECTIVE: To analyze the frequency of infection by human papilloma virus (HPV) and other agents like Candida sp., Gardnerella vaginalis and Trichomonas vaginalis in cytological smears. DESIGN STUDY: Retrospective study SETTING: A public tertiary referral center. SAMPLE: An analysis of 17,391 cytologies from outpatients seen between January 1997 and August 1998. The control group was made up of patients in the same age group and same period with no cytological evidence of HPV infection. Patients with a diagnosis of cervical intraepithelial neoplasia (CIN) II or III were excluded from this analysis. MAIN MEASUREMENTS: The diagnosis of HPV infection was made in accordance with the criteria of Schneider et al. and the diagnosis of Gardnerella vaginalis was made with a finding of clue cells. RESULTS: 390 (2.24%) had alterations consistent with infection by HPV, sometimes associated with CIN I. The results showed that Gardnerella vaginalis was the most frequent agent in women with HPV infection (23.6% versus 17.4%; P <0.05), while in the control group the most frequent agent was Candida sp. (23.9% versus 13.8%; p <0.001). CONCLUSION: In spite of this study being based solely on cytological criteria, in which specific HPV and Gardnerella diagnostic tests were not used, the cytological smear is widely used in clinical practice and the data presented in this investigation show that there is an association between Gardnerella vaginalis and HPV infection. It remains to be established whether the microorganisms favor each other.

scholarly journals PREVALÊNCIA DE Gardnerella vaginalis, Candida spp. e Trichomonas vaginalis EM EXAMES CITOPATOLÓGICOS DE GESTANTES ATENDIDAS NO HOSPITAL UNIVERSITÁRIO DE SANTA MARIA-RS

2011 ◽  
Vol 36 (1) ◽  
pp. 37
Author(s):  
Gabriela Bonfanti ◽  
Thissiane De Lima Gonçalves
Keyword(s):  
Prenatal Care ◽  
Pregnant Women ◽  
Trichomonas Vaginalis ◽  
Pap Smear ◽  
Gardnerella Vaginalis ◽  
Candida Spp ◽  
Pap Smear Test ◽  
Microbiological Agents ◽  
Santa Maria ◽  
Lactobacillus Spp

Introdução: Durante a gestação podem ocorrer distúrbios no mecanismo fisiológico do trato genital, composto por Lactobacillus spp., resultando em processos infecciosos determinados por agentes bacterianos. Tais infecções podem levar a doença pélvica inflamatória, parto prematuro e infecção fetal, sendo que o exame de Papanicolaou pode sugerir a presença desses agentes infecciosos. Objetivo: Verificar a prevalência dos agentes microbiológicos encontrados no Papanicolaou de gestantes atendidas no HUSM. Métodos: Foi realizado um levantamento do arquivo dos laudos citológicos do Laboratório do Hospital Universitário de Santa Maria (HUSM) no período de janeiro de 2005 a dezembro de 2008, das gestantes que realizaram o exame citopatológico durante o pré-natal. Resultados: Foram analisados 1344 laudos e observou-se que 59,82% das pacientes apresentaram flora bacteriana normal, ou seja, composta porLactobacillus spp. O total de floras alteradas foi de 40,17%. Dessas, 38,24% apresentaram Gardnerella vaginalis, 33,75% Candida spp., 5,92% Trichomonas vaginalis, 21,54% flora mista (cocos e outros bacilos) e 0,54% outros microorganismos como Leptothrix vaginalis e Fuseobacterium spp. Conclusão: Essas infecções estão associadas à complicações na gestação e nossos resultados mostraram uma alta prevalência de agentes patogênicos nas pacientes, confirmando a importância do acompanhamento pré-natal das gestantes para o diagnóstico e tratamento adequado dessas possíveis infecções.Descritores: gestantes, exame citopatológico, agentes microbiológicos.  Introduction: During pregnancy may occur disorders in the physiological mechanism of genital tract, consisting ofLactobacillus spp., resulting in infectious processes determined by bacterial agents. These infections can lead to pelvic inflammatory disease, preterm delivery and fetal infection. The Pap Smear test may suggest the presence of these infectious agents. Objective: To determinate the prevalence of microbiological agents found in the Pap smear test of pregnant women attending in HUSM. Methods: It was made survey of the file of the cytological laboratory of the Hospital Universitario de Santa Maria (HUSM) from January 2005 to December 2008, belonging to women, who underwent the examination of cytopathologic during prenatal care. Results: 1344 reports were analyzed and indicated that 59.82% of patients showed normal bacterial flora, which is composed of Lactobacillus spp. The total flora switched was 40.17%. Of these, 38.24% had Gardnerella vaginalis, 33.75% Candida spp., 5.92% Trichomonas vaginalis, 21.54% mixed flora (bacilli and cocci) and 0.54% other microorganisms such as Leptothrix vaginalis and Fuseobacteriumspp. Conclusion: The microbial infections are associated with complications during pregnancy, our results showed a high prevalence of pathogens in patients, confirming the importance of prenatal care of pregnant women for diagnosis and proper treatment of possible infections.Descriptors: pregnant women, cervical screening, microbiological agents.  

scholarly journals Molecular diagnosis of bacterial vaginosis: Prevalence of Gardnerella vaginalis and Atopobium vaginae in pregnant women

2018 ◽  
Vol 146 (7-8) ◽  
pp. 417-421
Author(s):  
Snezana Matic ◽  
Dane Nenadic ◽  
Jelena Cukic ◽  
Zeljko Mijailovic ◽  
Nevena Manojlovic ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Pregnant Women ◽  
Real Time ◽  
Vaginal Discharge ◽  
Gardnerella Vaginalis ◽  
Bacterial Number ◽  
Vaginal Smears ◽  
Vaginal Lactobacilli ◽  
Real Time Qpcr ◽  
Atopobium Vaginae

Introduction/Objective. Bacterial vaginosis (BV) is defined as disequilibrium of vaginal microbiota due to proliferation of Gram-negative/variable anaerobes and reduction/depletion of vaginal lactobacilli. Difficulties in interpreting microscopically categorized findings in diagnosis of BV need a molecular analysis of bacteria present in vaginal discharge of patients. In this regard, we performed real-time qPCR analysis of vaginal discharge samples with the goal to explore in which extent prevalence and amount of anaerobes, Gardnerella vaginalis and Atopobium vaginae, are related to findings obtained by microscopy. Methods. This study enrolled 111 asymptomatic pregnant women between 24 and 28 weeks of pregnancy. Gram-stained vaginal smears were evaluated microscopically. Afterwards, DNA of bacteria was extracted from Gram slides and real-time qPCR was performed with the aim to detect and quantify G. vaginalis and A. vaginae. Results. The data of our study showed that 53.2% of patients had normal results, while 20.7% and 26.1% of patients had intermediary (IMD) and BV results, respectively. G. vaginalis and A. vaginae were more frequently found in IMD and BV than in healthy patients; also, the average bacterial number of G. vaginalis and A. vaginae were significantly higher in BV and IMD than in the group with normal findings (p = 0.000). Comparing mutual relation of G. vaginalis and A. vaginae, the prevalence and number of G. vaginalis were in all groups significantly higher than A. vaginae. Conclusion. The data of our study have shown that in distinguishing normal from BV findings, quantification of bacteria may be more important than just molecular detection of bacteria.

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