scholarly journals EFFECT OF SILVER NITRATE ON IN VITRO REGENERATION AND ANTIOXIDANT RESPONSES OF OILSEED RAPE CULTIVARS (BRASSICA NAPUS L.)

2021 ◽  
Vol 10 (6) ◽  
Keyword(s):  
Brassica Napus ◽  
Silver Nitrate ◽  
Oilseed Rape ◽  
In Vitro Regeneration ◽  
Brassica Napus L ◽  
Antioxidant Responses

scholarly journals The Impact of Forest Fungi on Promoting Growth and Development of Brassica napus L.

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2475
Author(s):  
Grażyna B. Dąbrowska ◽  
Zuzanna Garstecka ◽  
Alina Trejgell ◽  
Henryk P. Dąbrowski ◽  
Wiktoria Konieczna ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Growth And Development ◽  
Plant Pathogens ◽  
Fungal Species ◽  
Brassica Napus L ◽  
Number Of Microorganisms ◽  
The Impact

Inoculation of plants with fungi has been shown to increase yields by improving germination, seedling vigor, plant growth, root morphogenesis, photosynthesis, and flowering through direct or indirect mechanisms. These mechanisms include solubilization and mineralization of nutrients, facilitating their uptake by plants, regulation of hormone balance, production of volatile organic compounds and microbial enzymes, suppression of plant pathogens, and mitigation of abiotic stresses. In the presented experiments, the effect of selected forest soil fungi on the growth and development of Brassica napus L. seedlings was investigated. Inoculation was carried out in vivo and in pot experiments with ectomycorrhizal and saprophytic fungi typical of forest soils: Collybia tuberosa, Clitocybe sp., Laccaria laccata, Hebeloma mesophaeum, and Cyathusolla. It was shown that all analyzed fungi produced IAA. In the in vitro experiment, B. napus inoculated with L. laccata showed stimulated root growth and greater number of leaves compared to control plants. A similar stimulatory effect on lateral root formation was observed in cuttings grown in pots in the presence of the C. olla fungus. In the pot experiment, the seedlings inoculated with the L. laccata fungus also showed increased growth of shoots and biomass. The effect of inoculation with the tested fungal strains, especially C. olla, on the growth and development of oilseed rape was probably indirect, as it also contributed to an increase in the number of microorganisms, especially soil bacteria. The expression of the metallothioneins in B. napus (BnMT1-BnMT3) varied depending on the fungal species. The presence of C. olla significantly increased BnMT2 expression in oilseed rape. It was found that BnMT1 expression increased and BnMT3 transcripts decreased in plants growing in the presence of L. laccata. This indicates the involvement of BnMT in the adaptation of oilseed rape to growth in fungi presence.

scholarly journals Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos

2019 ◽  
Vol 55 (No. 4) ◽  
pp. 162-165
Author(s):  
Miroslav Klíma ◽  
Eva Jozová ◽  
Irena Jelínková ◽  
Vratislav Kučera ◽  
Shengwu Hu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Restorer Gene ◽  
Brassica Napus L ◽  
Rf Gene ◽  
Dh Lines ◽  
Non Destructive ◽  
Selection Of

Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype comparisons. In 14 plants (12.2%) the phenotype did not match the genotype: two plants were marked as fertile, but without the Rf gene. Vice versa, in 12 sterile plants the Rf gene was determined. Nevertheless, the two fertile plants did not produce any pods after self-pollination. In total, 47 doubled haploid (DH) lines with good seed yield, carrying the Rf gene were harvested.  

Development of an Effective In Vitro Regeneration System for Ukrainian Breeding Winter Rape Brassica napus L.

2020 ◽  
Vol 54 (4) ◽  
pp. 341-346 ◽  
Author(s):  
I. S. Hnatyuk ◽  
O. I. Varchenko ◽  
M. V. Kuchuk ◽  
M. F. Parii ◽  
Yu. V. Symonenko
Keyword(s):  
Brassica Napus ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Winter Rape ◽  
Brassica Napus L

scholarly journals Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)

2015 ◽  
Vol 18 (1) ◽  
pp. 58-60 ◽  
Author(s):  
Lenka Havlíčková ◽  
Miroslav Klíma ◽  
Marie Přibylová ◽  
Alois Antonín Hilgert-Delgado ◽  
Vratislav Kučera ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Winter Oilseed Rape ◽  
Fertility Restorer ◽  
Brassica Napus L ◽  
Fertility Restorer Gene ◽  
Non Destructive ◽  
Selection Of

In vitro selection of herbicide resistance in microspore-derived embryos of oilseed rape (Brassica napus L.)

2006 ◽  
Vol 3 (1) ◽  
pp. 33-38
Author(s):  
Xu Ling ◽  
Zhang Guo-Qing ◽  
Gu Hong-Hui ◽  
He Yun ◽  
Zhou Wei-Jun
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Chromosome Doubling ◽  
Microspore Culture ◽  
Brassica Napus L ◽  
Regenerated Plants ◽  
Short Time ◽  
Selection Of

AbstractUsing four oilseed rape (Brassica napus L.) F1 hybrids (7039, 7040, 282 and 5102) as donor plants for microspore culture, the experiment was conducted to select glyphosate- and haloxyfop-resistant embryos through application of these substances to the cultural media with microspore-derived embryos in vitro. Genotypes 7039 and 7040 were used to select glyphosate-resistant regenerated plants, and genotypes 282 and 5102 to select haloxyfop-resistant plants. The embryos at cotyledonary stage were grown on glyphosate- and haloxyfop-containing MS-2 medium for 2 weeks. The non-resistant embryos collapsed after a short time, while the resistant ones turned green and survived for 2 weeks. Transferred into the normal MS-2 medium for further plant regeneration, the regenerated plants from green embryos showed tolerance to 0.25% sprayed glyphosate, indicating the effectiveness and reliability of this in vitro selection method. When the regenerated plants selected from 0.02% haloxyfop were sprayed with 0.05% haloxyfop, most of them grew well; however, the survival rate of the regenerated plants from 0.01% haloxyfop-containing medium was lower. The present experiment indicates that the use of 0.02% haloxyfop in the selection of haloxyfop-resistant plants was more promising than that of 0.01%. The chromosome doubling efficiency of regenerated plants reached 34% and 52% after being treated with 170 mg/l colchicine for 20 and 30 h, respectively.

In vitro shoot regeneration from commercial cultivars of Australian canola (Brassica napus L.)

2004 ◽  
Vol 55 (7) ◽  
pp. 753 ◽  
Author(s):  
Yan Zhang ◽  
Prem L. Bhalla
Keyword(s):  
Brassica Napus ◽  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Brassica Napus L ◽  
Shoot Initiation ◽  
Seedling Explants ◽  
Modern Breeding ◽  
In Vitro Shoot Regeneration ◽  
Number Of Shoots

Canola, (Brassica napus L.) is an important crop in Australia. Large genetic variability in the Australian canola cultivars is reflected by their diverse agronomic characteristics. Further improvement using modern breeding methods will lead to the generation of better canola varieties suited for Australian conditions. Genetic engineering relies on the development of efficient methods for regeneration of viable shoots from cultured tissues, and the successful application of transformation techniques. This study reports the in vitro shoot regeneration potential from seedling explants of 7 commercial genotypes (Dunkeld, Grouse, RK7, RI25, Oscar, Rainbow, and Monty) of Australian canola. Seedling explants of these genotypes were all responsive to shoot regeneration. Total number of shoots regenerated varied significantly among the 7 genotypes. Based on the number of shoots regenerated, Rainbow was found to be the most amenable to in vitro regeneration with 55% of cotyledon explants regenerating 2.47 shoots per explants on shoot initiation medium containing 6-benzylaminopurine (3 mg/L), 1-naphthylacetic acid (0.2 mg/L), and gibberellic acid (0.01 mg/L). Normal fertile canola plants from all the 7 genotypes were regenerated. The results obtained from this study will form the basis for genetic transformation studies.

scholarly journals Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens

2020 ◽  
Vol 21 (22) ◽  
pp. 8740
Author(s):  
Daria Chlebek ◽  
Artur Pinski ◽  
Joanna Żur ◽  
Justyna Michalska ◽  
Katarzyna Hupert-Kocurek
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Oilseed Rape ◽  
Plant Growth Promotion ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Genome Mining ◽  
Plant Colonization ◽  
Brassica Napus L

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.

Amino acid contents and transport in oilseed rape (Brassica napus L.) under different nitrogen conditions

Planta ◽  
2004 ◽  
Vol 221 (3) ◽  
pp. 328-338 ◽  
Author(s):  
Jens Tilsner ◽  
Nina Kassner ◽  
Christine Struck ◽  
Gertrud Lohaus
Keyword(s):  
Amino Acid ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Amino Acid Contents ◽  
Nitrogen Conditions
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