In vitro regeneration of shoot buds and plantlets from seedling root segments of Brassica napus L.

1989 ◽  
Vol 18 (1) ◽  
pp. 129-141 ◽  
Author(s):  
Kiran K. Sharma ◽  
Trevor A. Thorpe
Keyword(s):  
Brassica Napus ◽  
In Vitro Regeneration ◽  
Brassica Napus L ◽  
Shoot Buds ◽  
Seedling Root ◽  
Root Segments

Development of an Effective In Vitro Regeneration System for Ukrainian Breeding Winter Rape Brassica napus L.

2020 ◽  
Vol 54 (4) ◽  
pp. 341-346 ◽  
Author(s):  
I. S. Hnatyuk ◽  
O. I. Varchenko ◽  
M. V. Kuchuk ◽  
M. F. Parii ◽  
Yu. V. Symonenko
Keyword(s):  
Brassica Napus ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Winter Rape ◽  
Brassica Napus L

In vitro shoot regeneration from commercial cultivars of Australian canola (Brassica napus L.)

2004 ◽  
Vol 55 (7) ◽  
pp. 753 ◽  
Author(s):  
Yan Zhang ◽  
Prem L. Bhalla
Keyword(s):  
Brassica Napus ◽  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Brassica Napus L ◽  
Shoot Initiation ◽  
Seedling Explants ◽  
Modern Breeding ◽  
In Vitro Shoot Regeneration ◽  
Number Of Shoots

Canola, (Brassica napus L.) is an important crop in Australia. Large genetic variability in the Australian canola cultivars is reflected by their diverse agronomic characteristics. Further improvement using modern breeding methods will lead to the generation of better canola varieties suited for Australian conditions. Genetic engineering relies on the development of efficient methods for regeneration of viable shoots from cultured tissues, and the successful application of transformation techniques. This study reports the in vitro shoot regeneration potential from seedling explants of 7 commercial genotypes (Dunkeld, Grouse, RK7, RI25, Oscar, Rainbow, and Monty) of Australian canola. Seedling explants of these genotypes were all responsive to shoot regeneration. Total number of shoots regenerated varied significantly among the 7 genotypes. Based on the number of shoots regenerated, Rainbow was found to be the most amenable to in vitro regeneration with 55% of cotyledon explants regenerating 2.47 shoots per explants on shoot initiation medium containing 6-benzylaminopurine (3 mg/L), 1-naphthylacetic acid (0.2 mg/L), and gibberellic acid (0.01 mg/L). Normal fertile canola plants from all the 7 genotypes were regenerated. The results obtained from this study will form the basis for genetic transformation studies.

Biosynthesis of glucosinolates by microspore derived embryoids and plantlets in vitro of Brassica napus L.

Plant Science ◽  
1995 ◽  
Vol 112 (1) ◽  
pp. 107-115 ◽  
Author(s):  
M.C.M. Iqbal ◽  
G. Röbbelen ◽  
C. Möllers
Keyword(s):  
Brassica Napus ◽  
Brassica Napus L

Screening rhizobacteria for improving the growth, yield, and oil content of canola (Brassica napus L.)

2004 ◽  
Vol 55 (2) ◽  
pp. 187 ◽  
Author(s):  
H. N. Asghar ◽  
Z. A. Zahir ◽  
M. Arshad
Keyword(s):  
Brassica Napus ◽  
Grain Yield ◽  
Root Length ◽  
Oil Content ◽  
Growth Promotion ◽  
Brassica Napus L ◽  
Growth Promoting ◽  
1000 Grain Weight ◽  
Number Of Branches

One hundred rhizobacteria previously isolated from the rhizospheres of Brassica species were screened for their growth promoting activity in Brassica napus L. under gnotobiotic conditions. Results revealed that 58% of the rhizobacteria increased root length (up to 139%), 39% enhanced shoot length (up to 78%), and shoot weight (up to 72%) of Brassica napus L. Based upon growth promotion of B. napus seedlings under gnotobiotic conditions, 10 promising plant-growth-promoting rhizobacteria (PGPR) were selected and tested for their effectiveness in growth promotion, yield, and oil content of B. napus grown in pots. The pot trials revealed that inoculation with selected PGPR increased plant height, root length, number of branches per plant, stem diameter, number of pods per plant, 1000-grain weight, grain yield, and oil content over a range of 7–57% above the uninoculated control. These isolates were then assayed for their ability to produce auxins in vitro in the presence and absence of L-tryptophan. Regression analysis showed that in vitro auxin production by these bacteria was significantly related to the number of branches and oil content of B. napus. It is highly likely that improvement in growth and yield of the inoculated plants is due to an increase in the number of branches per plant, since there was a positive correlation of this growth parameter with the number of pods per plant, 1000-grain weight, grain yield, and seed oil content. Results indicated that simultaneous screening of rhizobacteria for growth promotion under gnotobiotic conditions and in vitro production of auxins could be a useful approach for selecting effective PGPR.

Sign in / Sign up

Export Citation Format

Share Document