scholarly journals OPTIMIZATION of a NEW MEDIUM of WHEY WATER and SWEET POTATO EXTRACT for DIFFERENT LACTOBACILLUS SPECIES by PLACKETT-BURMAN STATISTICAL DESIGN USING MINI –TAB16 SOFTWARE

Author(s):  
Amel Ahmed Ibrahim
2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins


2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Weni Tri Purnani

ABSTRAKASI merupakan sumber makanan yang mengandung nutrisi yang lengkap untuk bayi. Rendahnya cakupan ASI di Indonesia menyumbang akibat yang tidak baik bagi kesehatan bayi. Berdasarkan hasil survey pendahuluan, dari 10 ibu menyusui 6 (60%) diantaranya mengatakan bahwa pengeluaran ASI mereka tidak lancar. Salah satu solusi yang tepat untuk meningkatkan suplai ASI adalah dengan mengonsumsi daun ubi jalar yang dipercaya mengandung berbagai macam zat dan vitamin terutama vitamin A. Tujuan penelitian ini adalah mengetahui pengaruh pemberian rebusan daun ubi jalar terhadap kecukupan ASI pada ibu menyusui di Wilayah Kerja Puskesmas Campurejo Tahun 2016. Desain penelitian ini adalah pre eksperimental menggunakan rancangan pretest posttest design. Dalam penelitian ini diperoleh sampel 20 orang menggunakan teknik simple random sampling. Pengumpulan data dilakukan dengan menggunakan lembar kuesioner dan penilaian. uji hipotesis yang digunakan adalah uji peringkat bertanda dari wilcoxon dengan nilai signifikasi α = 0,05. Hasil uji statistik dari Wilcoxon Sign Rank diperoleh nilai p value = 0,000 dengan taraf kesalahan (α =0,05) dapat dikatakan p ≤ α maka dapat disimpulakan bahwa H0 ditolak dan H1 diterima. Hal ini berarti ada pengaruh pemberian rebusan daun ubi jalar terhadap kecukupan ASI pada ibu menyusui diwilayah kerja puskesmas campurejo kota Kediri tahun 2016. Berdasarkan hasil penelitian diharapkan bagi tenaga kesehatan dapat  dijadikan sebagai masukan untuk menggunakan daun ubi jalar untuk meningkatkan kecukupan ASI dalam pengembangan pengobatan tradisional. Kata Kunci : Kecukupan ASI, Daun Ubi jalar, Menyusui   ABSTRACTBreast milk is a food source that contains a complete nutritions for infants. Coverage of breast milk in Indonesia is still very low. Based on the preliminary results of the survey, from 10 breastfeeding mothers, there are 6 (60%) breastfeeding mom of them say that their breast milk isn’t enough for the babies. There’s a rightest solution to increase the breast milk supply, such as; the breastfeeding mom should eat or consume the sweet potato leaves that believed contains various nutritions and vitamins, especially vitamin A. The purpose of this study was to know the effect of giving sweet potato extract to breast milk adequacy on breastfeeding mothers in work area clinic of Campurejo Kediri City in 2016. The research design of this study was pre-experimental that using pre-test and post-test design. In this study obtained 20 samples of people that using simple random sampling technique. The collecting of data is done by using a questionnaires and assessment. The test hypothesis is marked on the Wilcoxon rank test with significance value α = 0.05. The result of statistical test Wilcoxon Sign Rank obtained p value = 0.000 with a standard error (α = 0.05) can be said p ≤ α then it means that H0 is rejected and H1 is accepted. This means that there is an effect of giving sweet potato extract to breast milk adequacy on breastfeeding mothers in work area clinic of Campurejo Kediri City in 2016. Based on the results is the researcher expecting for health workers can be used as input to use sweet potato leaves to improve the adequacy of breast milk in the development of traditional medicine. Keywords: Adequacy of Breast Milk, Sweet Potato Leaves, Breastfeeding.


2009 ◽  
Vol 7 (2) ◽  
pp. 68-76
Author(s):  
RETNATI RETNATI ◽  
M.A.M. ANDRIANI ◽  
GUSTI FAUZA

Retnati, Andriani MAM, Fauza G. 2009. The influence of addition of various sweet potatoes (Ipomea batatas) extract to total count of cells and antioxidant activity in yogurt. Biofarmasi 7: 68-76. The aim of this research was to determine the influence of addition of various sweet potatoes extract to the total count of cells and the antioxidant activity in yogurt. Yogurt was made from fresh milk, skim milk, white sweet potato, orange sweet potato, purple sweet potato, and pure culture of Streptococcus thermophilus 0040 and Lactobacillus bulgaricus 0041 in straight MRS agar. Fresh milk, skim milk powder (5%, b/v), and sweet potato extract (10%, v/v) was pasteurized at 90oC for 15 minutes, cooled to the temperature between 40-45oC, inoculated with 2.5% S. thermophilus and L. bulgaricus with a proportion of 1.4:1, and then incubated at a temperature of 40oC for 15 hours. Yogurt without an addition of sweet potato extract was used as control. The parameters measured in this experiment were the total count of cells with TPC (Total Plate Count) method and the antioxidant activity with DPPH (2,2- diphenyl-1-picrylhydrazyl) method. Samples were taken at one hour interval to examine the total count of cells, while the antioxidant activity was collected at three hours interval. The result of each analysis was plotted into graphics which describing the relation of total bacteria and antioxidant activity with fermentation time. ANOVA was employed to analyze the data. If there was a significant difference, it should be followed by Duncan Multiple Range Test (DMRT) at a significance level α=0.05. The result of this research showed that the addition of various sweet potatoes extracts increased the total count of cells and the antioxidant activity in yogurt. The total count of cells showed no significant different for each sample, it meant that the different colors in sweet potato did not influence the total count of cells. However, yogurt with orange and purple sweet potato extract addition had a significant difference on the antioxidant activity with control and yogurt with white sweet potato extract. In conclusion, the difference colors in sweet potato influenced in the antioxidant activity in yogurt significantly. Sweet potato is potential for milk substitute in yogurt production due to oligosaccharide content and antioxidant activity.


2020 ◽  
Vol 12 (1) ◽  
pp. 9-15
Author(s):  
Yusmarini Yusmarini ◽  
Shanti Fitriani ◽  
Vonny Setiaries Johan ◽  
Rahmayuni Rahmayuni ◽  
Vita F. Artanti

Tapai is one of the typical Indonesian fermented products. This research utilized Lactobacillus plantarum 1 RN2-53 and natural dyes made from plants in the production of cassava tapai. The objectives of this research were to improve the quality of probiotic cassava tapai and to obtain the chemical, microbiological and sensory characteristics of the cassava tapai. Natural dyes used were purple sweet potato extract, red dragon fruit extract and narrow-leaf pleomele extract. The results showed that cassava tapai made with the addition of Lactobacillus plantarum 1 RN2-53 and the addition of natural dyes from plants had different chemical and microbiological characteristics. Tapai made with the addition of red dragon fruit extract generally had a lower pH value and a higher total titrated acid compared to tapai made with the addition of purple sweet potato extract and narrow-leaf pleomele extract, but the alcohol content of tapai made with the addition of purple sweet potatoes extract was higher than the others. The number of lactic acid bacteria was around 109 and had met the probiotic food criteria.


Author(s):  
Meddy Setiawan ◽  
Fakih Nadhil

Cardiovascular disease is a disease caused by impaired functions of heart and blood vessel, one of those is coronary heart disease. This study aimed to identify the effect of purple sweet potato (Ipomoea batatas L) extract on MDA (Malondialdehyde) levels of male white rat (Rattus norvegicus Wistar strain) model of atherosclerosis. This research was an experimental study using Post-Test Only Control Group Design method, used 5 groups (positive control group, negative control, treatment 1, treatment 2, and treatment 3), each group contained 5 rats plus 2 reserve rats. ANOVA one way test obtained a significance of 0.00 (p <0.05). The regression test results showed the coefficient of determination R² = 0.71, which means a decrease in serum MDA levels of experimental animals is influenced by the dose of purple sweet potato extract administration as much as 71% while the remaining 29% can be influenced by factors outside the study. It can be concluded that purple sweet potato extract can affect serum MDA levels of experimental animals.


2019 ◽  
Vol 18 (1) ◽  
pp. 80-88 ◽  
Author(s):  
Achmad Noerkhaerin Putra ◽  
Yayu Romdhonah

ABSTRACT This study aimed to investigate the effects of probiotic (Bacillus NP5), prebiotic (extracted from Ipomoea batatas var. sukuh,), and synbiotic on growth and digestive enzyme activity of dumbo catfish (Clarias sp.). Four treatments with 3 replications were used in the experiment, i.e; control, probiotic (1% Bacillus NP5 in feed), prebiotic (2% sweet potato extract in feed), and synbiotic (1% probiotic and 2% prebiotic in feed). After 45 days of rearing, catfish that were given probiotic, prebiotic, and synbiotic showed significantly (P<0.05) higher growth than that of the control. The average results on digestive enzyme activities in probiotic, prebiotic, and synbiotic treatments were significantly different (P<0.05) from that of the control. The addition of synbiotic to diets showed the best result on final weight, specific growth rate, feed conversion ratio, protein retention, protein efficiency ratio, and protease activities among other treatments. Keywords: catfish, growth, probiotic, prebiotic, symbiotic  ABSTRAK Penelitian ini bertujuan untuk menginvestigasi pengaruh dari probiotik (Bacillus NP5), prebiotik (ekstrak ubi jalar varietas sukuh), dan sinbiotik terhadap pertumbuhan dan aktivitas enzim perncernaan ikan lele dumbo (Clarias sp.). Penelitian terdiri dari 4 perlakuan dan 3 kali ulangan, yakni: probiotik (Bacillus NP5 sebanyak 1% dalam pakan), prebiotik (ekstrak ubi jalar sebanyak 2% dalam pakan), dan sinbiotik (Bacillus NP5 1% + ekstrak ubi jalar sebanyak 2% dalam pakan). Setelah pemeliharaan selama 45 hari, ikan lele pada perlakuan dengan suplementasi probiotik, prebiotik, dan sinbiotik dalam pakan secara signifikan (P<0,05) menghasilkan pertumbuhan yang lebih baik dibandingkan dengan kontrol. Nilai aktivitas enzim pencernaan pada perlakuan probiotik, prebiotik, dan sinbiotik secara signifikan berbeda (P<0,05) dari kontrol. Penambahan sinbiotik pada pakan memberikan hasil terbaik pada parameter bobot akhir, laju pertumbuhan harian, FCR, retensi protein, rasio efisiensi protein, dan aktivitas enzim protease dibandingkan dengan perlakuan lainnya. Kata kunci: ikan lele, pertumbuhan, probiotik, prebiotik, sinbiotik.  


2018 ◽  
Vol 5 (2) ◽  
pp. 129-139
Author(s):  
Devi Craselly Sihombing ◽  
Ade Dwi Sasanti ◽  
Mohamad Amin

ABSTRACTSynbiotics are a balanced combination of probiotics and prebiotics. Synbiotics indirectly influence the population of bacteria in the digestive tract and survival of the fish. The purpose of this research is to determine the affection of synbiotic addition to diet for population of intestinal bacteria, feed efficiency, survival rate and growth of tilapia. This research conducted on October-December 2016 at Laboratorium Budidaya Perairan, Departement of Aquaculture, Faculty of Agriculture, Sriwijaya. This research methode used Completly Randomize Design with six treatments and three replications. Tilapia feed treated with different dose of sweet potato extract prebiotics. P0 treatment by feeding (commercial pellets without probiotics and prebiotics), P1 treatment (5 ml probiotic + 2,5 ml prebiotic/kg pellets), P2 treatment (5 ml probiotic + 5,0 ml prebiotic/kg pellets), P3 treatment (5 ml probiotic + 7,5 ml prebiotic/kg pellets), P4 treatment (5 ml probiotic + 10 ml prebiotic/kg pellets) and P5 treatment (5 ml probiotic + 12,5 ml prebiotic/kg pellets). Parameters to be observed is population of intestinal bacteria, feed efficiency, growth and survival rate of tilapia. The highest value population of intestinal bacteria is P5 treatment with 4,17x104 cfu/ml. P3 treatment has the highest value of feed efficiency 50,19%, growth of length 1,92 cm, growth of weight 8,87 gram and survival rate 88,89%. Keywords: synbiotic, probiotic, prebiotic, feed.


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