scholarly journals In vitro study of NCs/dyes complexes accumulation and dyes release kinetics in rat hepatocytes

2015 ◽  
Vol 22 (2) ◽  
pp. 199-206 ◽  
Author(s):  
S.L. Yefimova ◽  
◽  
◽  
Keyword(s):  
Release Kinetics ◽  
In Vitro Study ◽  
Rat Hepatocytes ◽  
Vitro Study

Bile salt-induced cytotoxicity and ursodeoxycholate cytoprotection: in-vitro study in perifused rat hepatocytes

1997 ◽  
Vol 9 (7) ◽  
pp. 703-709 ◽  
Author(s):  
Paolo Pazzi ◽  
Anna C. Puviani ◽  
Mauro Dalla Libera ◽  
Giovanni Guerra ◽  
Daniele Ricci ◽  
...  
Keyword(s):  
Bile Salt ◽  
In Vitro Study ◽  
Rat Hepatocytes ◽  
Vitro Study

scholarly journals In vitro study of new combinations for local antibiotic therapy with calcium sulphate - Near constant release of ceftriaxone offers new treatment options

2018 ◽  
Vol 3 (4) ◽  
pp. 212-221 ◽  
Author(s):  
Peter Wahl ◽  
Karolin Rönn ◽  
Marc Bohner ◽  
Laurent A Decosterd ◽  
Christoph Meier ◽  
...  
Keyword(s):  
Release Kinetics ◽  
Local Treatment ◽  
In Vitro Study ◽  
Calcium Sulphate ◽  
Vitro Study ◽  
Local Application ◽  
Dissolution Time ◽  
New Combinations ◽  
Small Reduction

Abstract. Introduction: Local application of antibiotics provides high concentrations at the site of interest, with minimal systemic toxicity. Carrier materials might help manage dead space. Calcium sulphate (CaSO4) has a dissolution time that only slightly exceeds the usually recommended duration of systemic antibiotic treatments. This in vitro study evaluates compatibility, release kinetics and antibacterial activity of new combinations of antibiotics with CaSO4 as carrier material.Methods: CaSO4 pellets added with 8% w/w antibiotic powder were exposed once in phosphate-buffered saline (PBS) solution and once in bovine plasma, in an elution experiment run over 6 weeks at 37 °C. Antibiotic elution was examined at various time points. Concentration was measured by liquid chromatography with tandem mass spectrometry. Antimicrobial activity was checked with an agar diffusion test.Results: Piperacillin-tazobactam, ceftazidime, cefepime, and meropenem showed fast reduction of concentration and activity. Flucloxacillin and cefuroxime remained present in relevant concentrations for 4 weeks. Ciprofloxacin, levofloxacin and clindamycin lasted for 6 weeks, but also at cell toxic concentrations. Ceftriaxone showed a near-constant release with only a small reduction of concentration from 130 to 75 mg/l. Elution profiles from PBS and plasma were comparable.Conclusion: CaSO4 provides new possibilities in the local treatment of bone and joint infections. Ceftriaxone appears to be of particular interest in combination with CaSO4. Release persists at clinically promising concentrations, and appears to have a depot-like slow release from CaSO4, with only a small reduction in activity and concentration over 6 weeks. To the best of our knowledge, such a particular persistent release never was described before, for any antibiotic in combination with a carrier material for local application.

scholarly journals Hydrogen Peroxide Release Kinetics of Four Tooth Whitening Products—In Vitro Study

Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7597
Author(s):  
Susana Dias ◽  
António Mata ◽  
João Silveira ◽  
Ruben Pereira ◽  
Angelo Putignano ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Release Kinetics ◽  
Pearson Correlation ◽  
In Vitro Release ◽  
In Vitro Study ◽  
Vitro Study ◽  
Tooth Whitening ◽  
Mean Values ◽  
Kinetics Of

Tooth whitening efficacy can be influenced by several factors, of which concentration and application time are two of the most important. This in vitro study aimed to evaluate the initial content and release kinetics of the hydrogen peroxide (HP) content, or the carbamide peroxide (CP) content as converted to its HP equivalent, of four tooth whitening products with different concentrations (6% HP, 16% CP, 10% CP, and 5% CP). Titrations with Cerium Sulphate IV were performed to determine HP concentration. HP release kinetics were evaluated by a spectrophotometric technique. The results were expressed as the mean values and 95% confidence interval of the percentage of hydrogen peroxide content during release kinetics. One sample t-test, one-way ANOVA, Tukey post hoc testing, and Pearson correlation testing were used, as appropriate, with a significance level of α = 0.05. The concentration of titrated HP was higher than that indicated by the manufacturers in all tested products (p < 0.01). At the minimum application times indicated by the manufacturers, all products released at least 85% of HP content; the gel containing 10% CP registered the lowest release at 85.49 (81.52–89.46). There was a significant HP release in all products during the application times indicated by the manufacturers. Further studies are needed to assess in vitro release kinetics.

scholarly journals Dopamine impairs functional integrity of rat hepatocytes through nuclear factor kappa B activity modulation: An in vivo, ex vivo, and in vitro study

2015 ◽  
Vol 21 (12) ◽  
pp. 1520-1532 ◽  
Author(s):  
Cheuk-Kwan Sun ◽  
Ying-Hsien Kao ◽  
Po-Huang Lee ◽  
Ming-Chang Wu ◽  
Kun-Cho Chen ◽  
...  
Keyword(s):  
Nuclear Factor Kappa B ◽  
Ex Vivo ◽  
In Vitro Study ◽  
Rat Hepatocytes ◽  
Vitro Study ◽  
Nuclear Factor ◽  
Functional Integrity ◽  
Nuclear Factor Kappa

scholarly journals Cytokine and Growth Factor Delivery from Implanted Platelet-Rich Fibrin Enhances Rabbit Achilles Tendon Healing

2020 ◽  
Vol 21 (9) ◽  
pp. 3221
Author(s):  
Chin-Chean Wong ◽  
Yu-Min Huang ◽  
Chih-Hwa Chen ◽  
Feng-Huei Lin ◽  
Yi-Yen Yeh ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Histological Analysis ◽  
Release Kinetics ◽  
Tendon Healing ◽  
In Vitro Study ◽  
Vitro Study ◽  
Platelet Rich Fibrin ◽  
Tendon Defect ◽  
Collagen Iii

Tendons are hypocellular and hypovascular tissues, and thus, their natural healing capacity is low. In this study, we sought to evaluate the efficacy of platelet-rich fibrin (PRF) to serve as a bioactive scaffold in promoting the healing of rabbit Achilles tendon injury. For in vitro study, the essence portion of PRF was determined through bioluminescent assay. Furthermore, we analyzed the time-sequential cytokines-release kinetics of PRF and evaluated their effects on tenocytes proliferation and tenogenic gene expressions. In animal study, the rabbit Achilles tendon defect was left untreated or implanted with normal/heat-denatured PRF scaffolds. Six weeks postoperatively, the specimens were evaluated through sonographic imaging and histological analysis. The results revealed significantly more activated platelets on bottom half of the PRF scaffold. Cytokine concentrations released from PRF could be detected from the first hour to six days. For the in vitro study, PRF enhanced cell viability and collagen I, collagen III, tenomodulin, and tenascin gene expression compared to the standard culture medium. For in vivo study, sonographic images revealed significantly better tendon healing in the PRF group in terms of tissue echogenicity and homogeneity. The histological analysis showed that the healing tissues in the PRF group had more organized collagen fiber, less vascularity, and minimal cartilage formation. In conclusion, bioactive PRF promotes in vitro tenocytes viability and tenogenic phenotypic differentiation. Administration of a PRF scaffold at the tendon defect promotes tissue healing as evidenced by imaging and histological outcomes.

Sign in / Sign up

Export Citation Format

Share Document