scholarly journals Cytokine and Growth Factor Delivery from Implanted Platelet-Rich Fibrin Enhances Rabbit Achilles Tendon Healing

2020 ◽  
Vol 21 (9) ◽  
pp. 3221
Author(s):  
Chin-Chean Wong ◽  
Yu-Min Huang ◽  
Chih-Hwa Chen ◽  
Feng-Huei Lin ◽  
Yi-Yen Yeh ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Histological Analysis ◽  
Release Kinetics ◽  
Tendon Healing ◽  
In Vitro Study ◽  
Vitro Study ◽  
Platelet Rich Fibrin ◽  
Tendon Defect ◽  
Collagen Iii

Tendons are hypocellular and hypovascular tissues, and thus, their natural healing capacity is low. In this study, we sought to evaluate the efficacy of platelet-rich fibrin (PRF) to serve as a bioactive scaffold in promoting the healing of rabbit Achilles tendon injury. For in vitro study, the essence portion of PRF was determined through bioluminescent assay. Furthermore, we analyzed the time-sequential cytokines-release kinetics of PRF and evaluated their effects on tenocytes proliferation and tenogenic gene expressions. In animal study, the rabbit Achilles tendon defect was left untreated or implanted with normal/heat-denatured PRF scaffolds. Six weeks postoperatively, the specimens were evaluated through sonographic imaging and histological analysis. The results revealed significantly more activated platelets on bottom half of the PRF scaffold. Cytokine concentrations released from PRF could be detected from the first hour to six days. For the in vitro study, PRF enhanced cell viability and collagen I, collagen III, tenomodulin, and tenascin gene expression compared to the standard culture medium. For in vivo study, sonographic images revealed significantly better tendon healing in the PRF group in terms of tissue echogenicity and homogeneity. The histological analysis showed that the healing tissues in the PRF group had more organized collagen fiber, less vascularity, and minimal cartilage formation. In conclusion, bioactive PRF promotes in vitro tenocytes viability and tenogenic phenotypic differentiation. Administration of a PRF scaffold at the tendon defect promotes tissue healing as evidenced by imaging and histological outcomes.

scholarly journals The effect of platelet-rich fibrin on normal dermal fibroblast proliferation after mitomycin-c treatment: An in vitro study

2021 ◽  
Vol 62 ◽  
pp. 473-476
Author(s):  
Ishandono Dachlan ◽  
Hendy Satrya Kurniawan ◽  
Aditya Wicaksana ◽  
Aditya Rifqi Fauzi ◽  
Firdian Makrufardi ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Dermal Fibroblast ◽  
In Vitro Study ◽  
Vitro Study ◽  
Fibroblast Proliferation ◽  
Platelet Rich Fibrin

scholarly journals Evaluation of histological and pH changes in platelet-rich fibrin and platelet-rich fibrin matrix: A In vitro study

2019 ◽  
Vol 10 (4) ◽  
pp. 652
Author(s):  
RajanikanthB Rajaram ◽  
Shruthi Nagaraja ◽  
Sylvia Mathew ◽  
C Pushpalatha ◽  
Anil Abraham ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Platelet Rich Fibrin ◽  
Ph Changes ◽  
Fibrin Matrix

Effect of Basic Fibroblast Growth Factor; An In Vitro Study of Tendon Healing

1997 ◽  
Vol 342 ◽  
pp. 239???247 ◽  
Author(s):  
Barbara P. Chan ◽  
Kai Ming Chan ◽  
Nicola Maffulli ◽  
Sarah Webb ◽  
Kenneth K.H. Lee
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Tendon Healing ◽  
In Vitro Study ◽  
Vitro Study ◽  
Fibroblast Growth

scholarly journals Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

2020 ◽  
Vol 35 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Solomiya Kyyak ◽  
Sebastian Blatt ◽  
Andreas Pabst ◽  
Daniel Thiem ◽  
Bilal Al-Nawas ◽  
...  
Keyword(s):  
Bone Substitute ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Substitute Material ◽  
Platelet Rich Fibrin ◽  
Osteoblast Activity ◽  
Substitute Material ◽  
Xenogenic Bone

The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for migration (day 10), ABSM-i-PRF/XBSM-i-PRF showed higher values compared to ABSM/XBSM with maximum values for ABSM-i-PRF and minimum values for XBSM. At days 3 and 7, the highest expression of AP was detected in ABSM-i-PRF/XBSM-i-PRF when compared to ABSM/XBSM, whereas at day 10, AP expression levels were elevated in ABSM-i-PRF/ABSM. The highest BMP-2 expression was seen in ABSM-i-PRF whereas OCN expression showed higher levels in ABSM-i-PRF/XBSM-i-PRF at days 3 and 7 with lowest expression for ABSM. Later on, elevated OC levels were detected for ABSM-i-PRF only. In conclusion, i-PRF in combination with ABSM enhances HOB activity when compared to XBSM-i-PRF or untreated BSM in vitro. Therefore, addition of i-PRF to ABSM and – to a lower extent – to XBSM may influence osteoblast activity in vivo.

Drug Delivery Assessment of a Novel Triple Antibiotic-Eluting Injectable Platelet-Rich Fibrin Scaffold: An in-vitro Study.

2020 ◽  
Vol 21 ◽  
Author(s):  
Azade Rafiee ◽  
Mahtab Memarpour ◽  
Sara Taghvamanesh ◽  
Forough Karami ◽  
Somayeh Karami ◽  
...  
Keyword(s):  
High Performance ◽  
In Vitro Study ◽  
Vitro Study ◽  
Burst Release ◽  
Platelet Rich Fibrin ◽  
Fibrin Scaffold ◽  
Antibiotic Release ◽  
Simultaneous Identification

Background: Intracanal disinfection is a critical, yet challenging goal for the long-term success in regenerative-based treatments. This in-vitro study aimed to assess the release profile of triple antibiotic-eluting injectable platelet-rich fibrin (I-PRF) constructs in 28 days. Methods: I-PRF scaffolds containing triple antibiotic mixture [metronidazole (MET), ciprofloxacin (CIP), and minocycline (MINO)] by immersion (group one), I-PRF scaffolds containing triple antibiotic mixture by integration (group two), and antibiotic-free I-PRF scaffolds (group three) were fabricated. The antibiotic release from the scaffolds was measured using a high performance liquid chromatography (HPLC) (the mobile phase of 0.1% formic acid and methanol (35:65 v/v), a C18 analytical column (150 × 4.6 mm, 5 μm) at a flow rate of 0.7 mL/min, at 25ºC) at days 1, 3, 7, 14, 21, and 28. Results: Retention times for MINO, CIP, and MET were achieved as 2.3, 2.6, and 3.1 min, respectively. The maximum UV absorbances for CIP, MET, and MINO were at 268 nm, 278 nm, and 350 nm, respectively. The results of the first group showed burst release within the first 24 hours followed by sustained maintenance of all three antibiotics up to 14 days. MINO and MET were still detectable in the third week. The second group could not sustainably release of the antibiotics. Conclusions: The developed method for the simultaneous identification, and quantification of each antibiotic in I-PRF was sensitive and quick. Overall, group one could take up the antibiotics in adequate quantities and then subsequently release them over the study period.

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