Technology of Regeneration of the Bone Tissue in a Rotational Bioreactor: Modeling of the Fluid Flow and Laser-Induced Fluorescence Diagnostics

2020 ◽  
Keyword(s):  
Fluid Flow ◽  
Bone Tissue ◽  
Laser Induced Fluorescence ◽  
Fluorescence Diagnostics ◽  
Bioreactor Modeling

A Model for Bone Resorption

2006 ◽  
Author(s):  
Lars Johansson ◽  
Ulf Edlund ◽  
Anna Fahlgren ◽  
Per Aspenberg
Keyword(s):  
Fluid Flow ◽  
Bone Resorption ◽  
Constitutive Model ◽  
Bone Tissue ◽  
Driving Force ◽  
Strain State ◽  
Bone Remodelling ◽  
Fluid Pressure ◽  
Fluid Flow Velocity ◽  
Surgical Implants

In the present paper a model for the resorption of bone, such as that observed at the interface between surgical implants and bone tissue, is developed. While there are many previous studies where models for bone remodelling calculations are proposed, these have been based on the stress or strain state of the bone tissue itself as the driving force for bone remodelling. We, instead, develop a constitutive model based on observations in recent experiments where it seems that fluid pressure, or possibly fluid flow velocity, is a major factor in the bone resorption process.

SIMULATION ON TISSUE DIFFERENTIATIONS FOR DIFFERENT ARCHITECTURE DESIGNS IN BONE TISSUE ENGINEERING SCAFFOLD BASED ON CELLULAR STRUCTURE MODEL

2015 ◽  
Vol 15 (03) ◽  
pp. 1550028 ◽  
Author(s):  
XIANBIN ZHANG ◽  
HE GONG
Keyword(s):  
Tissue Engineering ◽  
Fluid Flow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Compressive Loading ◽  
Three Dimensional ◽  
Mechanical Stimuli ◽  
Interstitial Fluid Flow ◽  
Unconfined Compression Test ◽  
Defect Sites

In bone tissue engineering, mechanical stimuli are among the key factors affecting cell proliferation and differentiation. This study aimed to investigate the effects of different inlet fluid velocities and axial strains on the differentiation of bone marrow mesenchymal stem cells (BMSCs) on the surface of scaffolds with different morphologies. Five three-dimensional bone scaffold architectures with 65% porosity were designed using typical cellular structural models of trabecular bone. Apparent compressive strains between 0% and 5% were applied to simulate an unconfined compression test. Strain distributions were analyzed on the wall surface of the solid model. The interstitial fluid flow at inlet velocities ranging between 0.01 mm/s and 1 mm/s was applied to interconnected pores, simulating a steady state flow in the scaffold. The shear stress distributions on the surface of the scaffolds were calculated. The differentiation of BMSCs on the surface of the scaffolds with different morphologies was predicted according to mechanoregulation theory. This study shows that different levels of mechanical stimuli can be generated as a result of different scaffold morphologies under compressive loading and fluid flow to satisfy the mechanical requirements for different bone defect sites.

Numerical Study of Granular Scaffold Efficiency to Convert Fluid Flow into Mechanical Stimulation in Bone Tissue Engineering

2015 ◽  
Vol 21 (9) ◽  
pp. 863-871 ◽  
Author(s):  
Magali Cruel ◽  
Morad Bensidhoum ◽  
Cécile Nouguier-Lehon ◽  
Olivier Dessombz ◽  
Pierre Becquart ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fluid Flow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Stimulation ◽  
Numerical Study

In Vivo Mesenchymal Stem Cell Proliferation in Response to Dynamic Fluid Flow Stimulation

2012 ◽  
Author(s):  
M. Hu ◽  
R. Yeh ◽  
M. Lien ◽  
Y. X. Qin
Keyword(s):  
Fluid Flow ◽  
Bone Tissue ◽  
Bone Adaptation ◽  
Hindlimb Suspension ◽  
Osteoporotic Bone ◽  
Hydraulic Stimulation ◽  
Structural Deterioration ◽  
Bone Fluid Flow ◽  
Cord Injury

Osteoporosis is a debilitating disease characterized as decreased bone mass and structural deterioration of bone tissue. Osteoporotic bone tissue turns itself into altered structure, which leads to weaker bones that are more susceptible for fractures. While often happening in elderly, long-term bed-rest patients, e.g. spinal cord injury, and astronauts who participate in long-duration spaceflights, osteoporosis has been considered as a major public health thread and causes great medical cost impacts to the society. Mechanobiology and novel stimulation on regulating bone health have long been recognized. Loading induced bone fluid flow, as a critical mechanotransductive promoter, has been demonstrated to regulate cellular signaling, osteogenesis, and bone adaptation [4]. As one of the factors that mediate bone fluid flow, intromedullary pressure (ImP) creates a pressure gradient that further influence the magnitude of mechanotransductory signals [5]. As for a potential translational development of ImP, our group has recently introduced a novel, non-invasive dynamic hydraulic stimulation (DHS) on bone structural enhancement. Its promising effects on inhibition of disuse bone loss has been shown with 2 Hz loading through a 4-week hindlimb suspension rat study followed by microCT analysis. At the cellular level, mesenchymal stem cells (MSCs) are defined by their self-renewal ability and that to potentially differentiate into the cells that form tissues such as bone [1]. To further elucidate the cellular effects of DHS and its potential mechanism on bone quality enhancement, the objective of this study was to measure MSC quantification in response to the in vivo mechanical signals driven by DHS.

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