Numerical Study of Granular Scaffold Efficiency to Convert Fluid Flow into Mechanical Stimulation in Bone Tissue Engineering

2015 ◽  
Vol 21 (9) ◽  
pp. 863-871 ◽  
Author(s):  
Magali Cruel ◽  
Morad Bensidhoum ◽  
Cécile Nouguier-Lehon ◽  
Olivier Dessombz ◽  
Pierre Becquart ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fluid Flow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Stimulation ◽  
Numerical Study

SIMULATION ON TISSUE DIFFERENTIATIONS FOR DIFFERENT ARCHITECTURE DESIGNS IN BONE TISSUE ENGINEERING SCAFFOLD BASED ON CELLULAR STRUCTURE MODEL

2015 ◽  
Vol 15 (03) ◽  
pp. 1550028 ◽  
Author(s):  
XIANBIN ZHANG ◽  
HE GONG
Keyword(s):  
Tissue Engineering ◽  
Fluid Flow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Compressive Loading ◽  
Three Dimensional ◽  
Mechanical Stimuli ◽  
Interstitial Fluid Flow ◽  
Unconfined Compression Test ◽  
Defect Sites

In bone tissue engineering, mechanical stimuli are among the key factors affecting cell proliferation and differentiation. This study aimed to investigate the effects of different inlet fluid velocities and axial strains on the differentiation of bone marrow mesenchymal stem cells (BMSCs) on the surface of scaffolds with different morphologies. Five three-dimensional bone scaffold architectures with 65% porosity were designed using typical cellular structural models of trabecular bone. Apparent compressive strains between 0% and 5% were applied to simulate an unconfined compression test. Strain distributions were analyzed on the wall surface of the solid model. The interstitial fluid flow at inlet velocities ranging between 0.01 mm/s and 1 mm/s was applied to interconnected pores, simulating a steady state flow in the scaffold. The shear stress distributions on the surface of the scaffolds were calculated. The differentiation of BMSCs on the surface of the scaffolds with different morphologies was predicted according to mechanoregulation theory. This study shows that different levels of mechanical stimuli can be generated as a result of different scaffold morphologies under compressive loading and fluid flow to satisfy the mechanical requirements for different bone defect sites.

FOAM VERSUS FIBRE SCAFFOLDS FOR MECHANICAL STIMULATION OF CELLS IN BONE TISSUE ENGINEERING

2008 ◽  
Vol 41 ◽  
pp. S385
Author(s):  
David Clarke ◽  
Carme Coll ◽  
Anthony Ryan ◽  
Aileen Crawford ◽  
Gwendolen Reilly
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Stimulation ◽  
Stimulation Of

Computational modeling of media flow through perfusion-based bioreactors for bone tissue engineering

2020 ◽  
Vol 234 (12) ◽  
pp. 1397-1408
Author(s):  
Hanieh Nokhbatolfoghahaei ◽  
Mahboubeh Bohlouli ◽  
Kazem Adavi ◽  
Zahrasadat Paknejad ◽  
Maryam Rezai Rad ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shear Stress ◽  
Fluid Flow ◽  
Computational Modeling ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Fluid Motion ◽  
Shear Loading ◽  
Perfusion Bioreactor ◽  
Perfusion Bioreactors

Bioreactor system has been used in bone tissue engineering in order to simulate dynamic nature of bone tissue environments. Perfusion bioreactors have been reported as the most efficient types of shear-loading bioreactor. Also, combination of forces, such as rotation plus perfusion, has been reported to enhance cell growth and osteogenic differentiation. Mathematical modeling using sophisticated infrastructure processes could be helpful and streamline the development of functional grafts by estimating and defining an effective range of bioreactor settings for better augmentation of tissue engineering. This study is aimed to conduct computational modeling for newly designed bioreactors in order to alleviate the time and material consuming for evaluating bioreactor parameters and effect of fluid flow hydrodynamics (various amounts of shear stress) on osteogenesis. Also, biological assessments were performed in order to validate similar parameters under implementing the perfusion or rotating and perfusion fluid motions in bioreactors’ prototype. Finite element method was used to investigate the effect of hydrodynamic of fluid flow inside the bioreactors. The equations used in the simulation to calculate the velocity values and consequently the shear stress values include Navier–Stokes and Brinkman equations. It has been shown that rotational fluid motion in rotating and perfusion bioreactor produces more velocity and shear stress compared with perfusion bioreactor. Moreover, implementing the perfusion together with rotational force in rotating and perfusion bioreactors has been shown to have more cell proliferation and higher activity of alkaline phosphatase enzyme as well as formation of extra cellular matrix sheet, as an indicator of bone-like tissue formation.

scholarly journals FLUID FLOW WITHIN BONE TISSUE ENGINEERING SCAFFOLDS: A COMPUTATIONAL AND EXPERIMENTAL STUDY

2008 ◽  
Vol 41 ◽  
pp. S83
Author(s):  
Silvia Truscello ◽  
Sebastian de Boodt ◽  
Toon Leroy ◽  
Jan Schrooten ◽  
Daniel Berckmans ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Experimental Study ◽  
Fluid Flow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Engineering Scaffolds
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