scholarly journals Role of osteopontin in decidualization and pregnancy success

Reproduction ◽  
2018 ◽  
Vol 155 (5) ◽  
pp. 423-432 ◽  
Author(s):  
Xiao-Bo Wang ◽  
Qian-Rong Qi ◽  
Kai-Lin Wu ◽  
Qing-Zhen Xie
Keyword(s):  
Signal Pathway ◽  
Human Endometrium ◽  
Implantation Failure ◽  
Successful Pregnancy ◽  
Secretory Phase ◽  
Failure Group ◽  
Blastocyst Implantation

OPN is essential for blastocyst implantation and placentation. Previous study found that miR181a was increased while miR181b was downregulated in endometrium during decidualization. However, the information regarding their effects on decidualization in human endometrium is still limited. Here, we report a novel role of OPN and miR181b in uterine decidualization and pregnancy success in humans. The expression of OPN was high in endometrium in secretory phase and in vitro decidualized hESC, whereas miR181b expression was low in identical conditions. Further analysis confirmed that OPN expression was upregulated by cAMP and C/EBPβ signal pathway, while downregulated by miR181b. Increased OPN expression could promote the expression of decidualization-related and angiogenesis-related genes. Conversely, the processes of decidualization and angiogenesis in hESC were compromised by inhibiting OPN expression in vitro. OPN expression was repressed in implantation failure group when compared with successful pregnancy group in IVF/ICSI-ET cycles. These findings add a new line of evidence supporting the fact that OPN is involved in decidualization and pregnancy success.

scholarly journals Medawar’s PostEra: Galectins Emerged as Key Players During Fetal-Maternal Glycoimmune Adaptation

2021 ◽  
Vol 12 ◽  
Author(s):  
Ellen Menkhorst ◽  
Nandor Gabor Than ◽  
Udo Jeschke ◽  
Gabriela Barrientos ◽  
Laszlo Szereday ◽  
...  
Keyword(s):  
Immune Cell ◽  
Successful Pregnancy ◽  
Fetal Health ◽  
The Past ◽  
Mammalian Embryogenesis ◽  
Carbohydrate Ligands ◽  
Membrane Bound

Lectin-glycan interactions, in particular those mediated by the galectin family, regulate many processes required for a successful pregnancy. Over the past decades, increasing evidence gathered from in vitro and in vivo experiments indicate that members of the galectin family specifically bind to both intracellular and membrane bound carbohydrate ligands regulating angiogenesis, immune-cell adaptations required to tolerate the fetal semi-allograft and mammalian embryogenesis. Therefore, galectins play important roles in fetal development and placentation contributing to maternal and fetal health. This review discusses the expression and role of galectins during the course of pregnancy, with an emphasis on maternal immune adaptions and galectin-glycan interactions uncovered in the recent years. In addition, we summarize the galectin fingerprints associated with pathological gestation with particular focus on preeclampsia.

scholarly journals Lycopene Treatment Ameliorates Amyloid Plaque Deposition and Memory Impairment in the APP/PS1 Mice

2021 ◽  
Author(s):  
Shanshan Ou ◽  
Yinchao Fang ◽  
Tong Wu ◽  
Jie Xu ◽  
Kaihua Guo
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Western Blot ◽  
Chronic Inflammation ◽  
Signal Pathway ◽  
Qualitative Assessment ◽  
Cell Cytotoxicity ◽  
Rt Pcr

Abstract Alzheimer’s disease (AD) is a neurodegenerative condition associated with oxidative stress and neuroinflammation. Lycopene has previously been shown to ameliorate neuroinflammation and exert protection against oxidative damage in neuroblastoma cells. The role of this compound in reversing cognitive dysfunction in AD has yet to be determined. The present study investigates the role of lycopene in AD with an in vitro Aβ1-42-induced cell cytotoxicity model as well as the in vivo APP/PS1 mouse model. The activation of Nrf2 signal pathway was assessed using western blot and RT-PCR. MDA, 8-OHdG, ROS, SOD, GHS and GSSG measurements were carried out using the specialized assay kits. The Morris water maze was used to examine qualitative assessment of memory and spatial learning. Immunofluorescence was used to visualize astrocytes and microglia activation as well as brain β-amyloid (Aβ) deposition. The NeuN positive cells were detected by immunofluorescence and western blot. Levels of cerebral cytokines were quantified using RT-PCR. Lycopene ameliorates oxidative damage in the Aβ1-42-triggered cell cytotoxicity model via Nrf2-ARE signal pathway activation, which is regulated by AKT-GSK3β pathway. In addition, lycopene improves the cognitive impairment and reduces the Aβ deposition. Mechanistically, lycopene attenuates neuron loss, decreases chronic inflammation and activates cerebral Nrf2-ARE signaling pathway in APP/PS1 mice. The results suggest that lycopene alleviates oxidative stress via AKT- Nrf2-ARE pathway. And early administration of lycopene improves cognitive deficits by reducing Aβ deposition, neuronal loss and decreasing the degree of chronic inflammation.

scholarly journals Remimazolam Protects Against LPS-Induced Endotoxicity Improving Survival of Endotoxemia Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaolei Liu ◽  
Shaoping Lin ◽  
Yiyue Zhong ◽  
Jiaojiao Shen ◽  
Xuedi Zhang ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Signal Pathway ◽  
Tlr4 Expression ◽  
Tnf Α ◽  
Sedative Drugs ◽  
Mapk Signal Pathway ◽  
Lps Treatment

Remimazolam is a new benzodiazepine of sedative drugs with an ultra-short-acting anesthetic effect, commonly used for critically ill patients (especially septic patients) in intensive care units (ICUs). Although some anesthetics have been reported to show certain anti-inflammatory effects, the role of remimazolam in inflammation is still remained unknown. Here, we studied the effects of remimazolam on macrophage in response to LPS both in vivo and in vitro. Interestingly, compared with LPS treatment group, remimazolam remarkably improved survival rate of endotoxemia mice and decreased the release of LPS-induced inflammatory mediators (such as TNF-α, IL-6, and IL-1β). We further found that remimazolam not only inhibited the activation of MAPK signal pathway at 15 min after LPS treatment but also disturbed Rab5a related TLR4 expression at cell surface in response to LPS at a later time. Such evidence suggests that remimazolam might be beneficial to septic patients who are suffering from uncontrolled inflammatory responses.

Clinical and immunological aspects of treatment women with repeated in-vitro fertilization failure

2014 ◽  
Vol 63 (5) ◽  
pp. 89-95
Author(s):  
Marina Ivanovna Krivonos ◽  
Marina Sabirovna Zaynulina ◽  
Sergey Vladimirovich Chepanov ◽  
Alexandr Vasilevich Selutin ◽  
Sergey Alekseevich Selkov ◽  
...  
Keyword(s):  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Implantation Failure ◽  
Fertilization Failure ◽  
Vitro Fertilization ◽  
Repeated Implantation Failure ◽  
Assisted Reproductive Treatment ◽  
Immune Pathology

This review presents the existing literature concerning repeated implantation failure in assisted reproductive technologies. There are also discussed the role of immune pathology in the occurrence of repeated implantation failure in assisted reproductive treatment and the prospects of improving implantation rates with intravenous immunoglobulin in women with different immune pathologies.

scholarly journals CO-CULTIVATION OF EMBRYOS WITH HUMAN ENDOMETRIUM: OPTIMIZATION OF IN VITRO FERTILIZATION

2012 ◽  
Vol 61 (4) ◽  
pp. 16-22 ◽  
Author(s):  
Eduard Karpovich Ailamazyan ◽  
Anna Olegovna Durnova ◽  
Viktoriya Olegovna Polyakova ◽  
Mariya Nikolaevna Sudalina ◽  
Igor Moiseevich Kvetnoy
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Current Knowledge ◽  
Human Endometrium ◽  
Human Embryos ◽  
Vitro Fertilization ◽  
Endometrial Cells ◽  
Methodological Aspects

The review is devoted to analysis of current knowledge about co-cultivation of human embryos with endometrial cells. The role of signaling molecules for regulation of implantation as well as embryo development is discussed. The special attention takes to the methodological aspects of cocultivation, which are promising for in vitro fertilization

IN VITRO METABOLISM OF [6,7-3H]NORETHYNODREL IN THE HUMAN ENDOMETRIUM AND THE MYOMETRIUM

1973 ◽  
Vol 74 (3) ◽  
pp. 576-591 ◽  
Author(s):  
K. Murugesan ◽  
V. Hingorani ◽  
K. R. Laumas
Keyword(s):  
Subcellular Fractions ◽  
Human Endometrium ◽  
In Vitro Metabolism ◽  
Supernatant Fraction ◽  
Chromatographic Mobility ◽  
Secretory Phase ◽  
Proliferative Phase ◽  
Polar Metabolites

ABSTRACT The human endometrium and the myometrium have the capacity to convert [6,7-3H]norethynodrel to norethindrone, 17α-ethynyl-3α,17β-dihydroxy-5-(10)-oestrene and two other unidentified metabolites. Under in vitro conditions, 80–90 % of 3H-norethynodrel was converted to its metabolites within 90 min. The metabolites formed were the same for both the endometrium and the myometrium. The amount of norethynodrel converted to its metabolites was more in the proliferative phase than in the secretory phase. Among the subcellular fractions of the myometrium, mitochondrial and microsomal fractions metabolised norethynodrel to norethindrone and two polar metabolites with low chromatographic mobility. In the myometrial 105 000 × g supernatant fraction, 17α-ethynyl-3α,17β-dihydroxy-5(10)-oestrene was formed in addition to norethindrone and the polar metabolites. The significance of the formation of norethindrone – a potent oral progestin as a product of the metabolism of norethynodrel and its possible action at the uterine level in the control of fertility is discussed.

scholarly journals The Role of Cytokines in Human Endometrium: The Inhibitory Effect of IL-1 and TNF α on in Vitro Decidualization and mRNA Expression of M-CSF, SCF and LIF in the Human Endometrium

1994 ◽  
Vol 41 (Supplement) ◽  
pp. S105-S115 ◽  
Author(s):  
HIDEHARU KANZAKI ◽  
KIMITOSHI IMAI ◽  
HIROSHI HATAYAMA ◽  
TAKUYA INOUE ◽  
KENJI KOJIMA ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Inhibitory Effect ◽  
Human Endometrium ◽  
Tnf Α

scholarly journals Effects of Acupuncture on Th1, Th2 Cytokines in Rats of Implantation Failure

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Juan Gui ◽  
Fan Xiong ◽  
Jing Li ◽  
Guangying Huang
Keyword(s):  
Acupuncture Group ◽  
Implantation Failure ◽  
Th2 Cytokines ◽  
Pregnant Rats ◽  
Failure Group ◽  
The Poor ◽  
Group A ◽  
Blastocyst Implantation ◽  
Th1 Cytokines ◽  
Cytokines Secretion

The aim is to explore the effect of acupuncture on Th1, Th2 cytokines in rats of implantation failure. Early pregnant rats were randomized into normal group (N), implantation failure group (M), acupuncture group (A), progestin group (H). The model was established with mifepristone. Samples of serum, endometrium were collected on Day 5, 6 and 8 of pregnancy. Compared with group M, the number of embryos was significantly higher in groups N, A and H; IL-1β, IL-2 protein in serum and endometrium were significantly lower in groups N, A and H, while IL-4, IL-10 were significantly higher in groups N, A and H; the endometrial IL-2, IL-4 mRNA were significantly lower in groups N, A and H, while IL-1β, IL-10 mRNA were significantly higher in groups N, A and H. Acupuncture could improve the poor receptive state of endometrium due to mifepristone by promoting Th2 cytokines secretion and inhibiting Th1 cytokines to improve blastocyst implantation.

scholarly journals The role of chronic endometritis in development of infertility in terms of etiological factors

2021 ◽  
pp. 22-27
Author(s):  
Ihor I. Chermak ◽  
Volodymyr I. Chermak ◽  
Mariia A. Matvian ◽  
Mansi Singh
Keyword(s):  
In Vitro Fertilization ◽  
Preterm Labor ◽  
Recurrent Miscarriage ◽  
Uterine Cavity ◽  
Implantation Failure ◽  
Chronic Endometritis ◽  
Vitro Fertilization ◽  
Persistent Inflammation

Chronic endometritis (CE) is a persistent inflammation of the inner lining of the uterine cavity caused by bacteria such as Enterococcus, Streptococcus, Staphylococcus, Enterobacteriaceae. A majority of CE produces no signs or sometimes mild symptoms, and the prevalence rate of CE is 10-15%. Several studies have shown that it is a condition frequently associated with repeated unexplained implantation failure at in vitro fertilization, recurrent miscarriage, as well as preterm labor. In this study, we reviewed how chronic endometritis plays an important role in the development of infertility. Also, we discuss the importance of hysteroscopic procedures in the diagnosis of chronic endometritis.

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