IN VITRO METABOLISM OF [6,7-3H]NORETHYNODREL IN THE HUMAN ENDOMETRIUM AND THE MYOMETRIUM

1973 ◽  
Vol 74 (3) ◽  
pp. 576-591 ◽  
Author(s):  
K. Murugesan ◽  
V. Hingorani ◽  
K. R. Laumas
Keyword(s):  
Subcellular Fractions ◽  
Human Endometrium ◽  
In Vitro Metabolism ◽  
Supernatant Fraction ◽  
Chromatographic Mobility ◽  
Secretory Phase ◽  
Proliferative Phase ◽  
Polar Metabolites

ABSTRACT The human endometrium and the myometrium have the capacity to convert [6,7-3H]norethynodrel to norethindrone, 17α-ethynyl-3α,17β-dihydroxy-5-(10)-oestrene and two other unidentified metabolites. Under in vitro conditions, 80–90 % of 3H-norethynodrel was converted to its metabolites within 90 min. The metabolites formed were the same for both the endometrium and the myometrium. The amount of norethynodrel converted to its metabolites was more in the proliferative phase than in the secretory phase. Among the subcellular fractions of the myometrium, mitochondrial and microsomal fractions metabolised norethynodrel to norethindrone and two polar metabolites with low chromatographic mobility. In the myometrial 105 000 × g supernatant fraction, 17α-ethynyl-3α,17β-dihydroxy-5(10)-oestrene was formed in addition to norethindrone and the polar metabolites. The significance of the formation of norethindrone – a potent oral progestin as a product of the metabolism of norethynodrel and its possible action at the uterine level in the control of fertility is discussed.

METABOLISM OF OESTRADIOL-17β AND OESTRONE IN THE HUMAN UTERUS

1975 ◽  
Vol 80 (4) ◽  
pp. 719-731 ◽  
Author(s):  
A. R. Krishnan ◽  
B. K. Bajaj ◽  
V. Hingorani ◽  
K. R. Laumas
Keyword(s):  
Metabolic Activity ◽  
Subcellular Fractions ◽  
Human Endometrium ◽  
Physiological Significance ◽  
Pyridine Nucleotides ◽  
Hormone Action ◽  
Human Uterus ◽  
Secretory Phase ◽  
Proliferative Phase ◽  
Metabolic Conversion

ABSTRACT A study of the metabolism of oestradiol in the human endometrium and myometrium of the proliferative and secretory phases of the cycle showed that the conversion of oestradiol to oestrone by endometrium in the proliferative phase was higher than that in the secretory phase. The decreased metabolic activity of the secretory phase endometrium was attributed to the influence of progesterone on the endometrium. The metabolic conversion of oestradiol to oestrone was enhanced when pyridine nucleotides were added to the system. The conversion of oestradiol to oestrone was maximum in the cytoplasmic and nuclear fractions of the endometrium. Furthermore, the conversion of oestradiol was low in all the subcellular fractions of the myometrium as compared with the endometrial subcellular fractions. The presence of co-factors increased the metabolic conversion of oestradiol to oestrone in the subcellular fractions of the endometrium. The presence of 17β-hydroxysteroid oxidoreductase was indicated in all the subcellular fractions. A correlation was found between the amount of oestradiol and oestrone bound to the receptors in the uterus and the rate of metabolism of oestradiol in the uterus. The physiological significance of metabolism of oestradiol and the hormone action are discussed.

scholarly journals In Vitro Metabolism of Isopropylated and tert-Butylated Triarylphosphate Esters Using Human Liver Subcellular Fractions

2020 ◽  
Vol 33 (6) ◽  
pp. 1428-1441 ◽  
Author(s):  
Allison L. Phillips ◽  
Nicholas J. Herkert ◽  
Jake C. Ulrich ◽  
Jessica H. Hartman ◽  
Matthew T. Ruis ◽  
...  
Keyword(s):  
Human Liver ◽  
Subcellular Fractions ◽  
In Vitro Metabolism

In vitro metabolism of C19 steroids in human endometrium

1982 ◽  
Vol 17 (6) ◽  
pp. 621-629 ◽  
Author(s):  
Virginia Hausknecht ◽  
Eduardo Lopez de la Osa ◽  
Erlio Gurpide
Keyword(s):  
Human Endometrium ◽  
In Vitro Metabolism

In vitro metabolism of [14C]rubratoxin B by rat hepatic subcellular fractions

1979 ◽  
Vol 17 (2) ◽  
pp. 111-116 ◽  
Author(s):  
P.D. Unger ◽  
M.Y. Siraj ◽  
A.W. Hayes
Keyword(s):  
Subcellular Fractions ◽  
In Vitro Metabolism

THE METABOLISM OF PROSTAGLANDIN E2 BY TISSUES FROM THE HUMAN UTERUS AND FOETO-PLACENTAL UNIT

1978 ◽  
Vol 87 (3) ◽  
pp. 632-642 ◽  
Author(s):  
Eve A. Willman ◽  
W. P. Collins
Keyword(s):  
Endometrial Tissue ◽  
Prostaglandin E ◽  
Human Uterus ◽  
Slight Effect ◽  
High Turnover ◽  
Secretory Phase ◽  
Proliferative Phase ◽  
Catabolic Enzymes ◽  
Decidual Tissue

ABSTRACT The biosynthesis and metabolism of prostaglandin E2 was studied in cell-free homogenates of tissues from the uterus and foeto-placental unit using specific in vitro methods. The results show that the synthesis of prostaglandin E2 is greater in endometrial tissue from the secretory phase (53.07 ± 39.05; ng/100 mg wet tissue/h) than from the proliferative phase of the uterine cycle. Furthermore, endometrial tissue forms more of this compound than myometrium (P < 0.005). During early pregnancy, synthesis is decreased (25.10 ± 16.62); at term, myometrium produces more prostaglandin E2 than decidua. During labour, however, decidual tissue accumulates more of this compound (52.73 ± 18.04) than either myometrium (34.71 ± 13.19) or cord (28.63 ± 11.71). The catabolic enzymes are most active in the placenta and chorion, followed by the cord, myometrium, decidua and amnion, but the differences have only a slight effect on the amounts of prostaglandin E2 which remain at the end of the incubation. The results suggest a high turnover of prostaglandin E2 in the decidua, myometrium and cord.

UPTAKE AND METABOLISM OF TRITIATED OESTRADIOL AND OESTRONE BY HUMAN ENDOMETRIAL AND MYOMETRIAL TISSUE IN VITRO

1974 ◽  
Vol 62 (1) ◽  
pp. 109-123 ◽  
Author(s):  
R. G. GABB ◽  
G. M. STONE
Keyword(s):  
Hydroxysteroid Dehydrogenase ◽  
Endometrial Tissue ◽  
Human Uterus ◽  
Secretory Phase ◽  
Proliferative Phase ◽  
Significant Difference ◽  
Degree Of Oxidation ◽  
Tissue Homogenates ◽  
Uptake And Metabolism

SUMMARY Human endometrial and myometrial tissues were incubated in vitro with [3H]oestradiol-17β and [3H]oestrone to study the uptake and interconversion of these two steroids by the tissues. Endometrial tissue displayed a higher capacity for oestrogen interconversion than myometrial tissue and the oxidation of oestradiol-17β to oestrone was favoured, rather than the reverse reaction. A greater degree of oxidation was found in tissue taken from uteri in the secretory phase than in the proliferative phase. A study of the distribution of radiometabolites between 'soluble' and 'particulate' fractions of tissue homogenates showed that a greater proportion of the tissue radioactivity was associated with the 'particulate' fraction in the proliferative phase than in the secretory phase. After incubation of endometrial tissue from the secretory phase with tritiated oestrogens there was evidence of the formation of chloroform-insoluble radiometabolites and some of these were tentatively identified as oestrogen sulphates. In a second experiment, the uptake and metabolism of the same two oestrogens by tissue from leiomyomata uteri (fibroids) and normal myometrial tissue were compared. No significant difference between these tissues was found. The results suggest that the levels of 17β-hydroxysteroid dehydrogenase in the human uterus may be dependent upon the levels of oestrogens in the blood. The lack of a difference in the treatment of oestrogens by fibroid and normal myometrial tissue suggests that these tissues may have a similar mechanism for the uptake and retention of oestrogens.

In vitro metabolism of opioid tetrapeptide agonists in various tissues and subcellular fractions from rats

Peptides ◽  
2001 ◽  
Vol 22 (4) ◽  
pp. 613-621 ◽  
Author(s):  
E Krondahl ◽  
H von Euler-Chelpin ◽  
A Orzechowski ◽  
G Ekström ◽  
H Lennernäs
Keyword(s):  
Subcellular Fractions ◽  
In Vitro Metabolism

scholarly journals Role of osteopontin in decidualization and pregnancy success

Reproduction ◽  
2018 ◽  
Vol 155 (5) ◽  
pp. 423-432 ◽  
Author(s):  
Xiao-Bo Wang ◽  
Qian-Rong Qi ◽  
Kai-Lin Wu ◽  
Qing-Zhen Xie
Keyword(s):  
Signal Pathway ◽  
Human Endometrium ◽  
Implantation Failure ◽  
Successful Pregnancy ◽  
Secretory Phase ◽  
Failure Group ◽  
Blastocyst Implantation

OPN is essential for blastocyst implantation and placentation. Previous study found that miR181a was increased while miR181b was downregulated in endometrium during decidualization. However, the information regarding their effects on decidualization in human endometrium is still limited. Here, we report a novel role of OPN and miR181b in uterine decidualization and pregnancy success in humans. The expression of OPN was high in endometrium in secretory phase and in vitro decidualized hESC, whereas miR181b expression was low in identical conditions. Further analysis confirmed that OPN expression was upregulated by cAMP and C/EBPβ signal pathway, while downregulated by miR181b. Increased OPN expression could promote the expression of decidualization-related and angiogenesis-related genes. Conversely, the processes of decidualization and angiogenesis in hESC were compromised by inhibiting OPN expression in vitro. OPN expression was repressed in implantation failure group when compared with successful pregnancy group in IVF/ICSI-ET cycles. These findings add a new line of evidence supporting the fact that OPN is involved in decidualization and pregnancy success.

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