scholarly journals Platelet Lysate as Replacement for Fetal Bovine Serum in Mesenchymal Stromal Cell Cultures

2013 ◽  
Vol 40 (5) ◽  
pp. 326-335 ◽  
Author(s):  
Karen Bieback
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Fetal Bovine

Serum-converted platelet lysate can substitute for fetal bovine serum in human mesenchymal stromal cell cultures

Cytotherapy ◽  
2013 ◽  
Vol 15 (12) ◽  
pp. 1458-1468 ◽  
Author(s):  
Mariluz P. Mojica-Henshaw ◽  
Pam jacobson ◽  
Julie Morris ◽  
Linda Kelley ◽  
Jan Pierce ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Fetal Bovine

Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation?

Biomaterials ◽  
2016 ◽  
Vol 76 ◽  
pp. 371-387 ◽  
Author(s):  
Thierry Burnouf ◽  
Dirk Strunk ◽  
Mickey B.C. Koh ◽  
Katharina Schallmoser
Keyword(s):  
Human Cell ◽  
Gold Standard ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine ◽  
Cell Propagation

scholarly journals Various factors affect lipopolysaccharide sensitization in cell cultures

BioTechniques ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 126-132
Author(s):  
Nan Yang ◽  
Don D Sin ◽  
Delbert R Dorscheid
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Signal Loss ◽  
The Media ◽  
Fetal Bovine

Commercially available lipopolysaccharide (LPS) is commonly used in research. Although protocols for its use are well established, we experienced a loss of LPS responsiveness in our cell cultures despite no obvious experimental changes. Our cell lines were stimulated with LPS and the media quantified for LPS responsiveness via an IL-8 ELISA. We discovered that the major cause of signal loss was differences in fetal bovine serum (FBS) formulation and concentration. One FBS formulation was notably better at eliciting an IL-8 signal than the second FBS, and 10% FBS in media was better at inducing LPS responsiveness than lower concentrations. We urge researchers to be aware of inherent variations in seemingly commonplace reagents as they may be unexpected sources of inconsistencies.

Platelet-Rich Plasma Can Replace Fetal Bovine Serum in Human Meniscus Cell Cultures

2013 ◽  
Vol 19 (11) ◽  
pp. 892-899 ◽  
Author(s):  
Veronica K. Gonzales ◽  
Eric L.W. de Mulder ◽  
Trix de Boer ◽  
Gerjon Hannink ◽  
Tony G. van Tienen ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Rich Plasma ◽  
Meniscus Cell ◽  
Fetal Bovine
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