CLINICAL STUDIES OF "BIG ACTH": ITS PHYSICO-CHEMICAL CHARACTERISTICS

1978 ◽  
Vol 89 (2) ◽  
pp. 221-231 ◽  
Author(s):  
Yukio Yamada ◽  
Seiki Ito ◽  
Masahiro Miyashita ◽  
Kenzo Kaneko ◽  
Toru Watanabe ◽  
...  
Keyword(s):  
Human Subjects ◽  
Gel Filtration ◽  
Disc Electrophoresis ◽  
Plasma Samples ◽  
Acth Secretion ◽  
Healthy Human ◽  
Physico Chemical ◽  
Healthy Human Subjects ◽  
Pituitary Glands ◽  
Stimulation Of

ABSTRACT The big ACTH fractions available from human plasma and pituitary glands and from porcine pituitary glands were physico-chemically characterized by gel filtration, disc electrophoresis and isoelectric separation. In the case of healthy human subjects, big ACTH fractions were isolated by gel filtration from plasma samples taken during states of acute ACTH hypersecretion such as the lysine-8-vasopressin, insulin or metopyrone tests though none of these fractions were isolated from plasma sampled under normal conditions. Even with no stimulation of ACTH secretion, patients with Cushing's disease gave plasma samples that contained an isolable big ACTH fraction, but such a fraction was hardly isolated from plasma taken from patient with Addison's disease. Both human pituitaries and porcine pituitaries contained an isolable big ACTH fraction. By a gel filtration analysis the molecular weight of the big ACTH was estimated to be higher than 20 000. Disc electrophoresis with an acrylamide gel indicated that big ACTH is strongly basic while small ACTH is more acidic than pH 8.3. Isoelectric separation revealed that the isoelectric point of human big ACTH is higher than pH 10.0 while that of small ACTH is about pH 6.8.

scholarly journals Facial Vibrotactile Stimulation Activates the Parasympathetic Nervous System: Study of Salivary Secretion, Heart Rate, Pupillary Reflex, and Functional Near-Infrared Spectroscopy Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Hisao Hiraba ◽  
Motoharu Inoue ◽  
Kanako Gora ◽  
Takako Sato ◽  
Satoshi Nishimura ◽  
...  
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Infrared Spectroscopy ◽  
Near Infrared ◽  
Human Subjects ◽  
Vibrotactile Stimulation ◽  
Functional Near Infrared Spectroscopy ◽  
Healthy Human ◽  
Healthy Human Subjects ◽  
Stimulation Of

We previously found that the greatest salivation response in healthy human subjects is produced by facial vibrotactile stimulation of 89 Hz frequency with 1.9 μm amplitude (89 Hz-S), as reported by Hiraba et al. (2012, 20011, and 2008). We assessed relationships between the blood flow to brain via functional near-infrared spectroscopy (fNIRS) in the frontal cortex and autonomic parameters. We used the heart rate (HRV: heart rate variability analysis in RR intervals), pupil reflex, and salivation as parameters, but the interrelation between each parameter and fNIRS measures remains unknown. We were to investigate the relationship in response to established paradigms using simultaneously each parameter-fNIRS recording in healthy human subjects. Analysis of fNIRS was examined by a comparison of various values between before and after various stimuli (89 Hz-S, 114 Hz-S, listen to classic music, and “Ahh” vocalization). We confirmed that vibrotactile stimulation (89 Hz) of the parotid glands led to the greatest salivation, greatest increase in heart rate variability, and the most constricted pupils. Furthermore, there were almost no detectable differences between fNIRS during 89 Hz-S and fNIRS during listening to classical music of fans. Thus, vibrotactile stimulation of 89 Hz seems to evoke parasympathetic activity.

scholarly journals Oxyphytosterols are present in plasma of healthy human subjects

2004 ◽  
Vol 91 (1) ◽  
pp. 101-106 ◽  
Author(s):  
André Grandgirard ◽  
Lucy Martine ◽  
Luc Demaison ◽  
Catherine Cordelet ◽  
Corinne Joffre ◽  
...  
Keyword(s):  
Human Subjects ◽  
Plasma Samples ◽  
Healthy Human ◽  
Human Samples ◽  
Human Volunteers ◽  
Healthy Human Subjects ◽  
Few Data ◽  
Derivatives Of

The oxidised derivatives of phytosterols (oxyphytosterols) were identified in plasma samples from thirteen healthy human volunteers, using MS. All the samples contained noticeable quantities of (24R)-5β,6β-epoxy-24-ethylcholestan-3β-ol (β-epoxysitostanol) and (24R)-ethylcholestan-3β,5α,6β-triol (sitostanetriol) and also trace levels of (24R)-5α,6α-epoxy-24-ethylcholestan-3β-ol (α-epoxysitostanol), (24R)-methylcholestan-3β,5α,6β-triol (campestanetriol) and (24R)-ethylch olest-5-en-3β-ol-7-one(7-ketositosterol). The amounts of these oxyphytosterols in plasma varied from 4·8 to 57·2 ng/ml. There are two possibilities concerning the origin of these compounds. First, they could come from the small amounts of oxyphytosterols in food. Second, they could originate from the in vivo oxidation of phytosterols in plasma. Very few data actually exist concerning these compounds. Their identification in human samples suggests that further research is necessary in this field.

Electrical Stimulation of Somatic Afferent Nerves in the Foot Increases Bladder Capacity in Healthy Human Subjects

2014 ◽  
Vol 191 (4) ◽  
pp. 1009-1013 ◽  
Author(s):  
Mang L. Chen ◽  
Christopher J. Chermansky ◽  
Bing Shen ◽  
James R. Roppolo ◽  
William C. de Groat ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Human Subjects ◽  
Bladder Capacity ◽  
Healthy Human ◽  
Afferent Nerves ◽  
Healthy Human Subjects ◽  
Stimulation Of

Comparison of human vocal cord movements during isocapnic hypoxia and hypercapnia

1982 ◽  
Vol 53 (1) ◽  
pp. 81-86 ◽  
Author(s):  
S. J. England ◽  
D. Bartlett ◽  
S. L. Knuth
Keyword(s):  
Vocal Cord ◽  
Human Subjects ◽  
Healthy Human ◽  
Peripheral Chemoreceptors ◽  
Airflow Resistance ◽  
Air Breathing ◽  
Healthy Human Subjects ◽  
Laryngeal Resistance ◽  
Stimulation Of

Respiratory vocal cord movements were analyzed in healthy human subjects during air breathing and in isocapnic hypoxia and hypercapnia. In the majority of experiments, expiratory glottic narrowing was substantially greater during hypoxia than during hypercapnia. Because ventilations were carefully matched under these two conditions, it is concluded that hypoxic stimulation of peripheral chemoreceptors favors expiratory glottic narrowing and leads to a relatively high laryngeal airflow resistance. In contrast, hypercapnia is accompanied by low expiratory laryngeal resistance. Similar rates of expiratory airflow with these two stimuli must be achieved by different balances of the factors that determine this flow.

A 1H NMR-based metabonomic study of urine and plasma samples obtained from healthy human subjects

2003 ◽  
Vol 33 (5) ◽  
pp. 1103-1115 ◽  
Author(s):  
E.M. Lenz ◽  
J. Bright ◽  
I.D. Wilson ◽  
S.R. Morgan ◽  
A.F.P. Nash
Keyword(s):  
1H Nmr ◽  
Human Subjects ◽  
Plasma Samples ◽  
Healthy Human ◽  
Healthy Human Subjects

Semiautomated analysis of ethanol and acetate in human plasma by head space gas chromatography

1986 ◽  
Vol 64 (6) ◽  
pp. 717-719 ◽  
Author(s):  
H. G. Giles ◽  
S. Meggiorini ◽  
E. I. Vidins
Keyword(s):  
Gas Chromatography ◽  
Methyl Acetate ◽  
Human Subjects ◽  
Plasma Samples ◽  
Healthy Human ◽  
Time Profiles ◽  
Head Space ◽  
Coefficients Of Variation ◽  
Healthy Human Subjects ◽  
Head Space Gas Chromatography

Plasma samples (0.5 mL) were analyzed for ethanol and acetate by head space gas chromatography using a Porapak QS® column (80–100 mesh). Acetate was esterified to methyl acetate simply by the addition of acidified methanol. The analytical ranges were 1.61–103 and 0.05–1.9 mM for ethanol and acetate, respectively. The within-run coefficients of variation did not exceed 4.7% for acetate and 2.7% for ethanol. After the oral administration of ethanol to two healthy human subjects, the concentration versus time profiles of plasma ethanol and acetate were determined. Acetate concentrations (0.4–0.9 mM) remained quite constant while ethanol was being metabolized and appeared not to be affected by the concentration of ethanol in the range 3–18 mM. The advantages of the method are speed and simplicity.

Chronic stress decreases circulating endocannabinoid 2-arachidonoylglycerol in healthy human subjects

2015 ◽  
Author(s):  
Buqing Yi ◽  
Igor Nichiporuk ◽  
Matthias Feuerecker ◽  
Gustav Schelling ◽  
Alexander Chouker
Keyword(s):  
Chronic Stress ◽  
Human Subjects ◽  
Healthy Human ◽  
Healthy Human Subjects
Sign in / Sign up

Export Citation Format

Share Document