Semiautomated analysis of ethanol and acetate in human plasma by head space gas chromatography

1986 ◽  
Vol 64 (6) ◽  
pp. 717-719 ◽  
Author(s):  
H. G. Giles ◽  
S. Meggiorini ◽  
E. I. Vidins
Keyword(s):  
Gas Chromatography ◽  
Methyl Acetate ◽  
Human Subjects ◽  
Plasma Samples ◽  
Healthy Human ◽  
Time Profiles ◽  
Head Space ◽  
Coefficients Of Variation ◽  
Healthy Human Subjects ◽  
Head Space Gas Chromatography

Plasma samples (0.5 mL) were analyzed for ethanol and acetate by head space gas chromatography using a Porapak QS® column (80–100 mesh). Acetate was esterified to methyl acetate simply by the addition of acidified methanol. The analytical ranges were 1.61–103 and 0.05–1.9 mM for ethanol and acetate, respectively. The within-run coefficients of variation did not exceed 4.7% for acetate and 2.7% for ethanol. After the oral administration of ethanol to two healthy human subjects, the concentration versus time profiles of plasma ethanol and acetate were determined. Acetate concentrations (0.4–0.9 mM) remained quite constant while ethanol was being metabolized and appeared not to be affected by the concentration of ethanol in the range 3–18 mM. The advantages of the method are speed and simplicity.

scholarly journals Oxyphytosterols are present in plasma of healthy human subjects

2004 ◽  
Vol 91 (1) ◽  
pp. 101-106 ◽  
Author(s):  
André Grandgirard ◽  
Lucy Martine ◽  
Luc Demaison ◽  
Catherine Cordelet ◽  
Corinne Joffre ◽  
...  
Keyword(s):  
Human Subjects ◽  
Plasma Samples ◽  
Healthy Human ◽  
Human Samples ◽  
Human Volunteers ◽  
Healthy Human Subjects ◽  
Few Data ◽  
Derivatives Of

The oxidised derivatives of phytosterols (oxyphytosterols) were identified in plasma samples from thirteen healthy human volunteers, using MS. All the samples contained noticeable quantities of (24R)-5β,6β-epoxy-24-ethylcholestan-3β-ol (β-epoxysitostanol) and (24R)-ethylcholestan-3β,5α,6β-triol (sitostanetriol) and also trace levels of (24R)-5α,6α-epoxy-24-ethylcholestan-3β-ol (α-epoxysitostanol), (24R)-methylcholestan-3β,5α,6β-triol (campestanetriol) and (24R)-ethylch olest-5-en-3β-ol-7-one(7-ketositosterol). The amounts of these oxyphytosterols in plasma varied from 4·8 to 57·2 ng/ml. There are two possibilities concerning the origin of these compounds. First, they could come from the small amounts of oxyphytosterols in food. Second, they could originate from the in vivo oxidation of phytosterols in plasma. Very few data actually exist concerning these compounds. Their identification in human samples suggests that further research is necessary in this field.

CLINICAL STUDIES OF "BIG ACTH": ITS PHYSICO-CHEMICAL CHARACTERISTICS

1978 ◽  
Vol 89 (2) ◽  
pp. 221-231 ◽  
Author(s):  
Yukio Yamada ◽  
Seiki Ito ◽  
Masahiro Miyashita ◽  
Kenzo Kaneko ◽  
Toru Watanabe ◽  
...  
Keyword(s):  
Human Subjects ◽  
Gel Filtration ◽  
Disc Electrophoresis ◽  
Plasma Samples ◽  
Acth Secretion ◽  
Healthy Human ◽  
Physico Chemical ◽  
Healthy Human Subjects ◽  
Pituitary Glands ◽  
Stimulation Of

ABSTRACT The big ACTH fractions available from human plasma and pituitary glands and from porcine pituitary glands were physico-chemically characterized by gel filtration, disc electrophoresis and isoelectric separation. In the case of healthy human subjects, big ACTH fractions were isolated by gel filtration from plasma samples taken during states of acute ACTH hypersecretion such as the lysine-8-vasopressin, insulin or metopyrone tests though none of these fractions were isolated from plasma sampled under normal conditions. Even with no stimulation of ACTH secretion, patients with Cushing's disease gave plasma samples that contained an isolable big ACTH fraction, but such a fraction was hardly isolated from plasma taken from patient with Addison's disease. Both human pituitaries and porcine pituitaries contained an isolable big ACTH fraction. By a gel filtration analysis the molecular weight of the big ACTH was estimated to be higher than 20 000. Disc electrophoresis with an acrylamide gel indicated that big ACTH is strongly basic while small ACTH is more acidic than pH 8.3. Isoelectric separation revealed that the isoelectric point of human big ACTH is higher than pH 10.0 while that of small ACTH is about pH 6.8.

A 1H NMR-based metabonomic study of urine and plasma samples obtained from healthy human subjects

2003 ◽  
Vol 33 (5) ◽  
pp. 1103-1115 ◽  
Author(s):  
E.M. Lenz ◽  
J. Bright ◽  
I.D. Wilson ◽  
S.R. Morgan ◽  
A.F.P. Nash
Keyword(s):  
1H Nmr ◽  
Human Subjects ◽  
Plasma Samples ◽  
Healthy Human ◽  
Healthy Human Subjects

Chronic stress decreases circulating endocannabinoid 2-arachidonoylglycerol in healthy human subjects

2015 ◽  
Author(s):  
Buqing Yi ◽  
Igor Nichiporuk ◽  
Matthias Feuerecker ◽  
Gustav Schelling ◽  
Alexander Chouker
Keyword(s):  
Chronic Stress ◽  
Human Subjects ◽  
Healthy Human ◽  
Healthy Human Subjects

Effect Of Water On Head Space-Gas Chromatography Odorants Signals From Orange Juice: A Possible Influence On Odor Intensity

LWT ◽  
2021 ◽  
pp. 111712
Author(s):  
David Reznik ◽  
Aviv Kaplan ◽  
Igal Gozlan ◽  
Gefen Ronen-Eliraz ◽  
Dror Avisar
Keyword(s):  
Gas Chromatography ◽  
Orange Juice ◽  
Odor Intensity ◽  
Effect Of Water ◽  
Head Space ◽  
Head Space Gas Chromatography

scholarly journals Acacia Gum Is Well Tolerated While Increasing Satiety and Lowering Peak Blood Glucose Response in Healthy Human Subjects

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 618
Author(s):  
Riley Larson ◽  
Courtney Nelson ◽  
Renee Korczak ◽  
Holly Willis ◽  
Jennifer Erickson ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Human Subjects ◽  
Area Under The Curve ◽  
Soluble Fiber ◽  
Healthy Human ◽  
Glucose Response ◽  
Blood Glucose Response ◽  
Acacia Gum ◽  
Fiber Treatment ◽  
Healthy Human Subjects

Acacia gum (AG) is a non-viscous soluble fiber that is easily incorporated into beverages and foods. To determine its physiological effects in healthy human subjects, we fed 0, 20, and 40 g of acacia gum in orange juice along with a bagel and cream cheese after a 12 h fast and compared satiety, glycemic response, gastrointestinal tolerance, and food intake among treatments. Subjects (n = 48) reported less hunger and greater fullness at 15 min (p = 0.019 and 0.003, respectively) and 240 min (p = 0.036 and 0.05, respectively) after breakfast with the 40 g fiber treatment. They also reported being more satisfied at 15 min (p = 0.011) and less hungry with the 40 g fiber treatment at 30 min (p = 0.012). Subjects reported more bloating, flatulence, and GI rumbling on the 40 g fiber treatment compared to control, although values for GI tolerance were all low with AG treatment. No significant differences were found in area under the curve (AUC) or change from baseline for blood glucose response, although actual blood glucose with 20 g fiber at 30 min was significantly less than control. Individuals varied greatly in their postprandial glucose response to all treatments. AG improves satiety response and may lower peak glucose response at certain timepoints, and it is well tolerated in healthy human subjects. AG can be added to beverages and foods in doses that can help meet fiber recommendations.

Diaphragmatic Fatigue after Exercise in Healthy Human Subjects

1993 ◽  
Vol 148 (6_pt_1) ◽  
pp. 1571-1575 ◽  
Author(s):  
M. Jeffery Mador ◽  
Ulysses J. Magalang ◽  
Angel Rodis ◽  
Thomas J. Kufel
Keyword(s):  
Human Subjects ◽  
Healthy Human ◽  
Healthy Human Subjects ◽  
Diaphragmatic Fatigue
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