DEMONSTRATION OF A PITUITARY GONADOTROPHIN HORMONE ACTIVITY IN THE MALE FOETAL MOUSE

1976 ◽  
Vol 83 (1) ◽  
pp. 158-165 ◽  
Author(s):  
G. Pointis ◽  
J. A. Mahoudeau
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Culture Medium ◽  
Biologically Active ◽  
Mouse Testis ◽  
Time Dependent ◽  
Hormone Activity ◽  
Organ Culture System ◽  
Testosterone Production

ABSTRACT The testosterone production by 18-days-old foetal mouse testis was measured in an organ culture system, by RIA in the culture medium. This production was time-dependent, and could be stimulated by ovine LH and age-matched foetal pituitary. The gonadotrophin activity derived from foetal pituitary appeared to be released into the culture medium as a limited reserve. These data clearly show that a biologically active gonadotrophin material is present in the pituitary of the 18-days-old mouse foetus.

scholarly journals Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mohit Parekh ◽  
Alessandro Ruzza ◽  
Stefano Ferrari ◽  
Diego Ponzin
Keyword(s):  
Organ Culture ◽  
Glucose Uptake ◽  
Culture System ◽  
Culture Medium ◽  
Organ Culture System ◽  
Before And After ◽  
Pas Staining ◽  
Organ Culture Medium ◽  
Preservation Conditions ◽  
Histological Staining

Purpose.To determine the optimum preservation conditions for preloading DMEK lenticules using organ culture system.Methods.8.5 mm DMEK lenticules were stripped and preserved with endothelium flap-in for 4 days at RT in an IOL cartridge that was blocked with rubber stoppers from each end. In C1, tissues were collected from tissue culture medium (TCM) and preserved in TCM. In C2, tissues were collected from transport medium (TCM + 6% dextran T500) (TM) and preserved in TM. In C3, tissues were collected from TCM and preserved in TM. Mortality, glucose uptake, histological staining, tight junctions and cell apoptosis were studied post-preservation.Results.Mortality in C1, C2, and C3 were 49.40%, 8.53%, and 27.74%, with 40.7%, 13%, and 41.8% uncovered areas. Glucose uptake (mg/mL) was 0.32, 0.43, and 0.56 in C1, C2, and C3. PAS staining showed presence of DM and endothelium in C2 but not in C1 and with fewer cells in C3. ZO-1 was expressed in all the conditions. Polymorphism was higher in C1 and C3. Mild apoptosis was observed in C3.Conclusions.Dextran may play an important role in preserving the endothelial cells before and after stripping for trifolded (endothelium-in) preloaded DMEK lenticules.

Ontogeny of pituitary gonadotrophin hormone activity and of testicular responsiveness to gonadotrophins in foetal rabbit

1980 ◽  
Vol 94 (3) ◽  
pp. 412-418 ◽  
Author(s):  
G. Veyssiere ◽  
M. Berger ◽  
Ch. Jean-Faucher ◽  
M. de Turckheim ◽  
Cl. Jean
Keyword(s):  
Organ Culture ◽  
Pituitary Gland ◽  
Sexual Differentiation ◽  
Culture System ◽  
Hormone Activity ◽  
Male And Female ◽  
Organ Culture System

Abstract. Synthesis and secretion of T and DHT by 18, 19 and 20 day old foetal rabbit testes were measured (RIA) in an organ culture system (M199). It was demonstrated that synthesis and secretion in vitro of T and DHT were greater at 19 and 20 days than at 18 days. The T and DHT production could be stimulated by ovine LH at 19 and 20 days but not at 18 days. The pituitaries of 19 and 20 day old male and female foetuses (but not those of foetuses aged 18 days) stimulate synthesis and secretion of T by 20 day old testis. A gonadotrophic factor, active on testicular T production, is present in the pituitary gland of 19 day old foetuses of both sexes. These findings indicate that the testicular responsiveness to LH and to the pituitary appears concomitantly with the pituitary gonadotrophic activity, between 19 and 20 days, just prior to the beginning of male sexual differentiation (20–25 days).

Evaluation of an in vitro mouse testis organ culture system for assessing male reproductive toxicity

2018 ◽  
Vol 111 (2) ◽  
pp. 70-77 ◽  
Author(s):  
Noriko Nakamura ◽  
Daniel T. Sloper ◽  
Pedro L. Del Valle
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Reproductive Toxicity ◽  
Mouse Testis ◽  
Organ Culture System

scholarly journals An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes

2013 ◽  
Vol 15 (5) ◽  
pp. R121 ◽  
Author(s):  
Dessislava Z Markova ◽  
Christopher K Kepler ◽  
Sankar Addya ◽  
Hallie B Murray ◽  
Alexander R Vaccaro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Global Gene Expression ◽  
Degenerative Changes ◽  
Organ Culture System

scholarly journals Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential

2011 ◽  
Vol 20 (8) ◽  
pp. 1244-1254 ◽  
Author(s):  
Bernice Jim ◽  
Thomas Steffen ◽  
Janet Moir ◽  
Peter Roughley ◽  
Lisbet Haglund
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

scholarly journals In the absence of a basal lamina, ameloblasts absorb enamel in a serumless and chemically defined organ culture system

2021 ◽  
Author(s):  
Retsu Ohki ◽  
M. Matsuki-Fukushima ◽  
K. Fujikawa ◽  
Mitsuori Mayahara ◽  
Kayo Matsuyama ◽  
...  
Keyword(s):  
Organ Culture ◽  
Basal Lamina ◽  
Culture System ◽  
Organ Culture System

Bench-Top Validation Tests for Tissue-Engineered Arteries

2000 ◽  
Author(s):  
Shawn Chin Quee ◽  
Hai-Chao Han ◽  
David N. Ku
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Vascular Grafts ◽  
Living System ◽  
Organ Culture System ◽  
Flow Pressure ◽  
Validation Tests

Abstract Standard tests are needed for evaluating and comparing the mechanical and biological functions of tissue engineered arteries and other vascular grafts. We propose an ex vivo organ culture system as a living system for testing tissue-engineered vascular grafts. This bench-top organ culture system mimics the physiological environment of arteries including the flow, pressure, and the axial stretch. Arterial mechanical properties and physiologic functions including compliance, burst pressure, and contractile functions can be assessed before an expensive long-term animal test is initiated. Test results of natural arteries indicate that organ culture is a valid model for comprehensive evaluation of tissue-engineered vascular grafts.

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