scholarly journals Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mohit Parekh ◽  
Alessandro Ruzza ◽  
Stefano Ferrari ◽  
Diego Ponzin
Keyword(s):  
Organ Culture ◽  
Glucose Uptake ◽  
Culture System ◽  
Culture Medium ◽  
Organ Culture System ◽  
Before And After ◽  
Pas Staining ◽  
Organ Culture Medium ◽  
Preservation Conditions ◽  
Histological Staining

Purpose.To determine the optimum preservation conditions for preloading DMEK lenticules using organ culture system.Methods.8.5 mm DMEK lenticules were stripped and preserved with endothelium flap-in for 4 days at RT in an IOL cartridge that was blocked with rubber stoppers from each end. In C1, tissues were collected from tissue culture medium (TCM) and preserved in TCM. In C2, tissues were collected from transport medium (TCM + 6% dextran T500) (TM) and preserved in TM. In C3, tissues were collected from TCM and preserved in TM. Mortality, glucose uptake, histological staining, tight junctions and cell apoptosis were studied post-preservation.Results.Mortality in C1, C2, and C3 were 49.40%, 8.53%, and 27.74%, with 40.7%, 13%, and 41.8% uncovered areas. Glucose uptake (mg/mL) was 0.32, 0.43, and 0.56 in C1, C2, and C3. PAS staining showed presence of DM and endothelium in C2 but not in C1 and with fewer cells in C3. ZO-1 was expressed in all the conditions. Polymorphism was higher in C1 and C3. Mild apoptosis was observed in C3.Conclusions.Dextran may play an important role in preserving the endothelial cells before and after stripping for trifolded (endothelium-in) preloaded DMEK lenticules.

DEMONSTRATION OF A PITUITARY GONADOTROPHIN HORMONE ACTIVITY IN THE MALE FOETAL MOUSE

1976 ◽  
Vol 83 (1) ◽  
pp. 158-165 ◽  
Author(s):  
G. Pointis ◽  
J. A. Mahoudeau
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Culture Medium ◽  
Biologically Active ◽  
Mouse Testis ◽  
Time Dependent ◽  
Hormone Activity ◽  
Organ Culture System ◽  
Testosterone Production

ABSTRACT The testosterone production by 18-days-old foetal mouse testis was measured in an organ culture system, by RIA in the culture medium. This production was time-dependent, and could be stimulated by ovine LH and age-matched foetal pituitary. The gonadotrophin activity derived from foetal pituitary appeared to be released into the culture medium as a limited reserve. These data clearly show that a biologically active gonadotrophin material is present in the pituitary of the 18-days-old mouse foetus.

scholarly journals An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes

2013 ◽  
Vol 15 (5) ◽  
pp. R121 ◽  
Author(s):  
Dessislava Z Markova ◽  
Christopher K Kepler ◽  
Sankar Addya ◽  
Hallie B Murray ◽  
Alexander R Vaccaro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Global Gene Expression ◽  
Degenerative Changes ◽  
Organ Culture System

scholarly journals Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential

2011 ◽  
Vol 20 (8) ◽  
pp. 1244-1254 ◽  
Author(s):  
Bernice Jim ◽  
Thomas Steffen ◽  
Janet Moir ◽  
Peter Roughley ◽  
Lisbet Haglund
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

scholarly journals In the absence of a basal lamina, ameloblasts absorb enamel in a serumless and chemically defined organ culture system

2021 ◽  
Author(s):  
Retsu Ohki ◽  
M. Matsuki-Fukushima ◽  
K. Fujikawa ◽  
Mitsuori Mayahara ◽  
Kayo Matsuyama ◽  
...  
Keyword(s):  
Organ Culture ◽  
Basal Lamina ◽  
Culture System ◽  
Organ Culture System

Bench-Top Validation Tests for Tissue-Engineered Arteries

2000 ◽  
Author(s):  
Shawn Chin Quee ◽  
Hai-Chao Han ◽  
David N. Ku
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Vascular Grafts ◽  
Living System ◽  
Organ Culture System ◽  
Flow Pressure ◽  
Validation Tests

Abstract Standard tests are needed for evaluating and comparing the mechanical and biological functions of tissue engineered arteries and other vascular grafts. We propose an ex vivo organ culture system as a living system for testing tissue-engineered vascular grafts. This bench-top organ culture system mimics the physiological environment of arteries including the flow, pressure, and the axial stretch. Arterial mechanical properties and physiologic functions including compliance, burst pressure, and contractile functions can be assessed before an expensive long-term animal test is initiated. Test results of natural arteries indicate that organ culture is a valid model for comprehensive evaluation of tissue-engineered vascular grafts.

Axial Stretch Increases Cell Proliferation in Arteries in Organ Culture

2000 ◽  
Author(s):  
Hai-Chao Han ◽  
Raymond P. Vito ◽  
Kristin Michael ◽  
David N. Ku
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Organ Culture ◽  
Vascular Function ◽  
Culture System ◽  
Carotid Arteries ◽  
Ex Vivo ◽  
Axial Stretch ◽  
Organ Culture System ◽  
Physiological Flow

Abstract To study the effect of axial stretch on vascular function and wall remodeling, porcine carotid arteries were cultured under conditions of physiological flow and elevated axial stretch in an ex vivo organ culture system. Smooth muscle cell proliferation was measured by bromodeoxyuridine index. Results showed that cell proliferation was significantly increased in the highly stretched arteries when compared to the normally stretched arteries. This may indicate the feasibility of stimulating new arterial growth by stretching natural arteries.

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