Evaluation of an in vitro mouse testis organ culture system for assessing male reproductive toxicity

2018 ◽  
Vol 111 (2) ◽  
pp. 70-77 ◽  
Author(s):  
Noriko Nakamura ◽  
Daniel T. Sloper ◽  
Pedro L. Del Valle
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Reproductive Toxicity ◽  
Mouse Testis ◽  
Organ Culture System

In vivo and in vitro Study of the Effects of Vitamin A Deficiency on Rat Third Molar Development

1986 ◽  
Vol 65 (12) ◽  
pp. 1445-1448 ◽  
Author(s):  
S.S. Harris ◽  
J.M. Navia
Keyword(s):  
Vitamin A ◽  
Organ Culture ◽  
Culture System ◽  
Vitamin A Deficiency ◽  
Third Molar ◽  
In Vitro Study ◽  
Organ Culture System ◽  
Vitamin A Status

We have examined the effect of in vivo vitamin A status on subsequent rat third molar formation and mineralization in an in vitro organ culture system. Vitamin A deficiency imposed during an eight-day in vitro period caused effects very similar to those of vitamin A deficiency imposed on rats in vivo. Analysis of the data also demonstrates that retinoic acid is capable of reversing the interference in mineralization of third molars induced by vitamin A deficiency in the organ culture system.

Usefulness of the organ culture system in the in vitro diagnosis of Celiac Disease: A multicenter study

2006 ◽  
Vol 38 ◽  
pp. S80-S81
Author(s):  
A. Picarelli ◽  
M. Di Tola ◽  
L. Sabbatella ◽  
M.C. Anania ◽  
A. Calabrò ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Organ Culture ◽  
Multicenter Study ◽  
Culture System ◽  
Organ Culture System ◽  
In Vitro Diagnosis

DEMONSTRATION OF A PITUITARY GONADOTROPHIN HORMONE ACTIVITY IN THE MALE FOETAL MOUSE

1976 ◽  
Vol 83 (1) ◽  
pp. 158-165 ◽  
Author(s):  
G. Pointis ◽  
J. A. Mahoudeau
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Culture Medium ◽  
Biologically Active ◽  
Mouse Testis ◽  
Time Dependent ◽  
Hormone Activity ◽  
Organ Culture System ◽  
Testosterone Production

ABSTRACT The testosterone production by 18-days-old foetal mouse testis was measured in an organ culture system, by RIA in the culture medium. This production was time-dependent, and could be stimulated by ovine LH and age-matched foetal pituitary. The gonadotrophin activity derived from foetal pituitary appeared to be released into the culture medium as a limited reserve. These data clearly show that a biologically active gonadotrophin material is present in the pituitary of the 18-days-old mouse foetus.

Growth hormone and somatostatin directly inhibit gastric ghrelin secretion. An in vitro organ culture system

2007 ◽  
Vol 30 (9) ◽  
pp. RC22-RC25 ◽  
Author(s):  
L. M. Seoane ◽  
O. Al-Massadi ◽  
F. Barreiro ◽  
C. Dieguez ◽  
F.F Casanueva
Keyword(s):  
Growth Hormone ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System ◽  
In Vitro Organ Culture ◽  
Ghrelin Secretion

scholarly journals Phenotypic Mapping of The Chicken Embryonic Thymic Microenvironment Developing Within an Organ Culture System

1993 ◽  
Vol 3 (2) ◽  
pp. 147-158 ◽  
Author(s):  
Natalie J. Davidson ◽  
Richard L. Boyd
Keyword(s):  
Organ Culture ◽  
Mhc Class Ii ◽  
Culture System ◽  
Antigen Expression ◽  
Cell Types ◽  
Normal Embryo ◽  
Organ Culture System ◽  
Stromal Component ◽  
Epithelial Antigens

The chicken thymic microenvironment, as it developed in an embryonic thymus organ culture system, was phenotypically mapped using a panel of mAb defining both epithelial and nonepithelial stromal cell antigens. We have previously reported that thymocyte proliferation and differentiation will proceed for up to 6–8 days in thymus organ culture, hence demonstrating the functional integrity of the thymic microenvironmentin vitro. During this time, the stromal component reflected that of the normal embryo with cortical and medullary epithelial areas readily identifiable by both morphology and surface-antigen expression. An abundance of subcapsular and cortical epithelial antigens was detected in the cultured thymus, particularly those normally expressed by the epithelium lining the capsule, trabeculae, and vascular regions (type epithelium) in the adult and embryonic thymus. Medullary epithelial antigens developed in organ culture, although were present in lower frequency than observed in the age-matched embryonic thymus. MHC class II expression by both epithelial and nonepithelial cells was maintained at high levels throughout the culture period. With increasing time in culture, the ratio of epithelial to nonepithelial cells decreased, concurrent with a decrease in thymocyte frequency and suggestive of a bidirectional interaction between these two cell types. Thus, a functionally intact thymic microenvironment appears to be maintained in embryonic thymus organ culture, a model that is currently being exploited to assess the role of stromal antigens, as defined by our mAb, in the process of thymopoiesis.

THE EFFECT OF OESTROGENS ON THE RESPONSE OF BONE TO PARATHYROID HORMONE IN VITRO

1972 ◽  
Vol 54 (1) ◽  
pp. 107-117 ◽  
Author(s):  
D. ATKINS ◽  
JOAN M. ZANELLI ◽  
M. PEACOCK ◽  
B. E. C. NORDIN
Keyword(s):  
Parathyroid Hormone ◽  
Citric Acid ◽  
Organ Culture ◽  
Culture System ◽  
Glucose Consumption ◽  
Organ Culture System ◽  
Mouse Calvaria ◽  
Ethinyl Oestradiol

SUMMARY An organ culture system was used to examine the effects of oestrogens on the response of 5-day-old mouse calvaria to parathyroid hormone (PTH). Parathyroid hormone released calcium and phosphate from the bone and this was associated with an increase in glucose consumption, an accumulation of citric acid and an inhibition of citrate oxidation. Oestradiol, oestriol, oestrone and ethinyl oestradiol all inhibited the PTH-induced release of calcium. The accumulation of citrate was prevented without the PTH-induced block on citrate oxidation being removed, and this was explained in terms of a reduction in glycolysis. Oestradiol, oestriol and oestrone appeared to be of equal potency. However, ethinyl oestradiol was active at much lower doses but appeared to be toxic at higher levels.

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