RELATIONSHIP BETWEEN OESTROGEN, PROSTAGLANDIN F2α AND HISTAMINE IN DELAYED IMPLANTATION IN THE MOUSE

1976 ◽  
Vol 81 (4) ◽  
pp. 801-807 ◽  
Author(s):  
S. K. Saksena ◽  
I. F. Lau ◽  
M. C. Chang
Keyword(s):  
Single Injection ◽  
Intraperitoneal Administration ◽  
Cell Formation ◽  
Prostaglandin F2α ◽  
Decidual Cell ◽  
Mouse Uterus ◽  
Subcutaneous Injections ◽  
Ovariectomized Mice ◽  
Complete Reversal ◽  
Pregnant Mice

ABSTRACT Treatment of pregnant mice ovaricctomized on day 3 (23.00-2330 h) with progesterone (1 mg/day/mouse, from day 4 through 11 post coitum (p.c.)) and oestrogen (single injection of 0.05 αg on day 8 p. c.) induced implantation in all the animals. Implantation was inhibited in animals following three subcutaneous injections of indomethacin given on the morning and afternoon of day 8 and the morning of day 9 p. c. A single injection of histamine on day 8 p. c. partially compensated the effect of indomethacin. A complete reversal of the antifertility effect of indomethacin was achieved following intraperitoneal administration of histamine (2 αg at noon of day 8 p. c.) and PGF2α (20 μg on the morning and afternoon of day 8 p. c.). This study suggests that in the mouse uterus indomethacin impairs the physiological changes which are required for the decidual cell formation and implantation. It is likely that an injection of oestradiol in the progesterone treated ovariectomized mice triggers the release of PGF2α and histamine and that these compounds are involved in the chain of events leading to implantation of the blastocysts.

EFFECTS OF ADRENALECTOMY ON REPRODUCTION IN THE MOUSE

1980 ◽  
Vol 86 (1) ◽  
pp. 155-164
Author(s):  
BARBARA M. WHITE ◽  
A. A. GIDLEY-BAIRD ◽  
C. W. EMMENS
Keyword(s):  
Single Injection ◽  
Sesame Seed ◽  
Linear Increase ◽  
Prednisolone Acetate ◽  
Methyl Prednisolone ◽  
Ovariectomized Mice ◽  
Significant Difference ◽  
Sesame Seed Oil ◽  
Pregnant Mice ◽  
Desoxycorticosterone Acetate

Survival of mice treated with sesame seed oil after adrenalectomy was very low and suggested no beneficial effect, whereas treatment with progesterone improved the chances of survival. Treatment with desoxycorticosterone acetate (DOCA) and methyl prednisolone acetate also increased the number of animals surviving after adrenalectomy. The corticosteroids were significantly more effective in ensuring survival than was progesterone. There was no significant difference in survival between mice receiving a single injection of 10 mg DOCA and those being given an injection of 1·0 mg DOCA per day for 3 days after the operation. To ensure minimum interference of exogenous corticosteroid with the experimental investigation, animals routinely received only a single injection of 1·0 mg DOCA after the operation. The chance of survival after adrenalectomy was higher in pregnant than in non-pregnant mice. There was a significant linear increase in survival during the first 5 days of pregnancy. Progesterone and prolactin both appeared to be involved in increasing the chance of survival in adrenalectomized pregnant mice. Adrenalectomy had no effect on the number of mice mating and ovulating. Adrenalectomized mice were apparently having normal cycles and 4 weeks after adrenalectomy they were able to mate and ovulate. Compensatory ovulation was seen in hemi-ovariectomized mice and was not abolished by adrenalectomy. Implantation was also unaffected by the operation.

Some biological activities of cronolone and medroxyprogesterone acetate in the uterus of the sheep, mouse and rabbit

1989 ◽  
Vol 1 (2) ◽  
pp. 157 ◽  
Author(s):  
X Zhang ◽  
BG Miller
Keyword(s):  
Biological Activity ◽  
Medroxyprogesterone Acetate ◽  
Biological Activities ◽  
Decidual Cell ◽  
Cell Reaction ◽  
Limited Growth ◽  
Mouse Uterus ◽  
Breeding Activity ◽  
Ovariectomized Mice

Cronolone (9 alpha-fluoro-11 beta-hydroxy-17 alpha-acetoxypregn-4-ene-3,20-dione) is widely employed to regulate breeding activity in the ewe, but its biological activity in the uterus of this and most other species has not been studied. In this study several in vivo uterus-related activities of cronolone have been examined in the sheep, mouse and rabbit. In some experiments the corresponding activities of medroxyprogesterone acetate (6 alpha-methyl-17 alpha-acetoxypregn-4-ene-3,20-dione, MAP) were also examined. Cronolone maintained pregnancy in ovariectomized ewes but not in ovariectomized mice and rabbits; it terminated pregnancy in some mice and in all rabbits that were receiving daily progesterone treatment. Cronolone could not sensitize the mouse uterus for the induction of the decidual-cell reaction or block the induction of such sensitivity by progesterone, but did support limited growth of the oil-stimulated horn after sensitization with progesterone. Cronolone induced uteroglobin secretion by rabbit endometrium. It was concluded that, whereas MAP is a potent progestogen in the sheep, mouse and rabbit, cronolone is a progestogen in the sheep and rabbit only. In the mouse and especially the rabbit, cronolone has other, non-progestational activities, which block pregnancy.

HORMONE SECRETION DURING EARLY PREGNANCY IN THE MOUSE

1969 ◽  
Vol 45 (1) ◽  
pp. 57-65 ◽  
Author(s):  
C. A. FINN ◽  
L. MARTIN
Keyword(s):  
Cell Division ◽  
Hormone Secretion ◽  
Hormone Treatment ◽  
Ovarian Hormones ◽  
Decidual Cell ◽  
Cell Reaction ◽  
Mouse Uterus ◽  
Small Doses ◽  
Pregnant Mice ◽  
Characteristic Manner

SUMMARY The distribution of cell division in the tissues of the mouse uterus changes in a characteristic manner during the first few days of pregnancy due to changes in the secretion of ovarian hormones. Pregnant mice were ovariectomized at various times and attempts made to reproduce the pattern of cell division with exogenous hormones. The schedule of hormone injections producing a pattern of uterine mitoses which most closely approached that of pregnancy suggested that secretion of both oestrogen and progesterone started about 48 hours after mating and continued, possibly increasing, until after implantation had started. This conflicts with the oestrogen surge hypothesis. Further experiments were carried out to determine the optimum hormone treatment schedules for sensitivity to induction of the oil decidual cell reaction. Multiple small doses of oestradiol allowed the production of greater deciduomas than a single dose, thus again suggesting that the oestrogen surge hypothesis may not be correct.

A REFRACTORY PERIOD FOLLOWING OESTROGENIC STIMULATION OF CELL DIVISION IN THE MOUSE UTERUS

1969 ◽  
Vol 44 (1) ◽  
pp. 121-126 ◽  
Author(s):  
C. A. FINN ◽  
L. MARTIN ◽  
JANET CARTER
Keyword(s):  
Cell Division ◽  
Refractory Period ◽  
Stromal Cell ◽  
Single Injection ◽  
Mouse Uterus ◽  
Ovariectomized Mice ◽  
Stimulation Of

SUMMARY A single injection of oestradiol given to ovariectomized mice pretreated with progesterone for several days stimulates a large increase in cell division in the uterine stroma which reaches a maximum 24 hr. later. A second injection 48 hr. after the first, also stimulates stromal cell division, but a second injection 12–36 hr. after the first does not. This failure to respond is not due simply to depletion of the stock of cells ripe for division. Rather it appears that the uterus becomes insensitive to further stimulation for approximately 36 hr. Although a second injection 24 hr. after the first injection does not increase stromal cell division, it does lengthen the refractory period so that a third injection given at 48 hr. has no effect.

HYPERTROPHY AND HYPERPLASIA IN THE MOUSE UTERUS AFTER OESTROGEN TREATMENT: AN AUTORADIOGRAPHIC STUDY

1973 ◽  
Vol 56 (1) ◽  
pp. 133-NP ◽  
Author(s):  
L. MARTIN ◽  
C. A. FINN ◽  
GAIL TRINDER
Keyword(s):  
Cell Death ◽  
Dna Synthesis ◽  
Proliferative Response ◽  
Autoradiographic Study ◽  
Luminal Epithelium ◽  
Uterine Tissue ◽  
Mouse Uterus ◽  
Oestrogen Treatment ◽  
Ovariectomized Mice

SUMMARY The uteri of untreated ovariectomized mice consisted almost entirely of myometrium and connective tissue stroma. After oestrogenic stimulation these tissues underwent marked hypertrophy, but showed little proliferation. The luminal epithelium underwent marked hyperplasia, with most cells dividing twice to quadruple cell numbers by 35–40 h, when they made up 10–12% of the uterine tissue volume and 20% of the total uterine cell population. The proliferative response was rapid, highly synchronized and short-lived. The number of cells incorporating [3H]thymidine first increased 8·5 h after oestradiol-17β and by 13–16 h 60–70% were engaged in DNA synthesis. Up to 21 h cell-death was minimal. From 21 h onwards the proliferation rate declined and the rate of cell death increased. A second injection of oestrogen prevented the rise in death rate and produced a second smaller burst of DNA synthesis. Cells in DNA synthesis or mitosis were insensitive to oestrogen. A smaller proliferative response occurred in the glands: only 25% of cells entered DNA synthesis after the first injection of oestradiol and none after the second. Gland cells appeared to die in situ and there was no evidence that they migrated into the luminal epithelium.

The hormonal control and morphology of blastocyst invasion in the mouse uterus in vitro

Development ◽  
1975 ◽  
Vol 34 (2) ◽  
pp. 299-310
Author(s):  
P. S. Grant ◽  
I. Ljungkvist ◽  
O. Nilsson
Keyword(s):  
Hormonal Control ◽  
Mouse Uterus ◽  
Chemically Defined Media ◽  
Fibrillar Material ◽  
Ovariectomized Mice ◽  
Defined Media ◽  
Stromal Tissue ◽  
Ultrastructural Effect

When mature and immature uteri from ovariectomized mice were cultured in chemically defined media, blastocyst invasion occurred in the presence of progesterone but not in media containing only oestradiol. The invaded stromal tissue did not decidualize unless the uteri were taken from mice pretreated with progesterone and oestradiol. Fibrillar material was, however, concentrated in the stromal tissue adjacent to the invasive trophoblast. Neither progesterone nor oestradiol, had any ultrastructural effect on the endometrium of cultured uteri from ovariectomized mice, other than closure of uterine lumina in the presence of progesterone. Embryogenesis became abnormal, probably owing to the failure of formation of a suitable implantation chamber.

Synchronization of oestrus and subsequent fertility in cattle, using the prostaglandin F2α analogue, ICI 80996 (cloprostenol)

1976 ◽  
Vol 16 (81) ◽  
pp. 437 ◽  
Author(s):  
H Hearnshaw
Keyword(s):  
Artificial Insemination ◽  
Luteal Phase ◽  
Prostaglandin F2α ◽  
Optimum Time ◽  
Subcutaneous Injections ◽  
Late Luteal Phase ◽  
Initial Injection ◽  
Subsequent Fertility ◽  
Subsequent Cycle ◽  
Cycle Lengths

The prostaglandin analogue, ICI 80996 (cloprostenol), was administered subcutaneously to synchronize oestrus in cattle. One injection, or two injections 12 days apart, of 400 pg gave optimal results (78 and 72 per cent synchronized respectively) for the Hereford cross heifers used. Heifers treated with cloprostenol from days one to five of the cycle, did not respond to the first injection, but did so to a second injection given 12 days later. A greater percentage of heifers, treated in the mid or late luteal phase of the cycle, exhibited oestrus after the first injection of cloprostenol than after the second injection. The percentage of these heifers showing induced oestrus varied from 44 to 100 per cent. There was a peak in the incidence of oestrus between 49 and 72 hours after injection but the distribution ranged from 24 to 120 hours. Peripheral blood progesterone concentrations, palpation of the ovaries and subsequent cycle lengths, indicated that the cloprostenol had no adverse effects in the majority of heifers. However, about 30 per cent of the heifers that responded to an initial injection of cloprostenol given in the mid luteal phase of the cycle failed to respond to a second injection. Some of these heifers had abnormal progesterone profiles and subsequent reproductive patterns. Fertility, following one or two subcutaneous injections of cloprostenol and artificial insemination at the set times of 72 and 96 hours, was 30 and 46 per cent respectively. However, if only heifers inseminated at the optimum time were considered, fertility at pregnancy diagnosis (73 and 65 per cent) and calving (53 and 57 per cent) was acceptable.

scholarly journals Interactions Between Oestradiol 3,17ß and Progesterone on the Induction and Growth of Deciduomata in Ovariectomized Mice

1969 ◽  
Vol 22 (3) ◽  
pp. 689 ◽  
Author(s):  
KW Humphrey
Keyword(s):  
Single Injection ◽  
Hormonal Control ◽  
Peanut Oil ◽  
Critical Parameters ◽  
Uterine Receptivity ◽  
Ovariectomized Mice ◽  
Progesterone Treatment ◽  
Before And After

The hormonal control of uterine sensitivity in progesterone-treated, ovariectomized mice was investigated by stimulating the deciduoma reaction by various means. Uterine sensitivity reached a peak on day 5 of progesterone treatment and then declined. Oestradiol before and after stimulation significantly increased the weight of the deciduomata induced by crushing, bradykinin, and compound 48/80 and increased uterine sensitivity so that intraluminal peanut oil could induce deciduomata. A single injection of 0�024 fJ-g of oestradiol 8 hr before intraluminal peanut oil also greatly increased uterine receptivity to oil. The dose of oestradiol and the time of injection of oestradiol and progesterone were critical parameters. Surprisingly, the hormonal requirements for uterine sensitivity to intraluminal peanut oil are more stringent than for transferred blastocysts.

Sign in / Sign up

Export Citation Format

Share Document