BESTIMMUNG VON TESTOSTERON IM PERIPHEREN BLUT VON SCHWEIN UND RIND MIT EINER DOPPELISOTOPEN-DERIVAT-VERDÜNNUNGSMETHODE

1970 ◽  
Vol 64 (2) ◽  
pp. 377-384 ◽  
Author(s):  
B. Hoffmann ◽  
R. Claus ◽  
H. Karg
Keyword(s):  
Isotope Dilution ◽  
Peripheral Blood ◽  
Derivative Method ◽  
Double Isotope

ABSTRACT Using a double-isotope-dilution derivative method combined with a cristallisation technique testosterone was measured in pig and bovine peripheral blood. In the male pig the values varied from 0.94 to 1.61 μg per 100 ml plasma and were 7- resp. 11-times higher than in castrated and female animals. Values increased with age. The concentration measured in a bull was 0.59 μg per 100 ml plasma.

A DOUBLE ISOTOPE DERIVATIVE METHOD FOR THE DETERMINATION OF ALDOSTERONE IN PERIPHERAL PLASMA

1961 ◽  
Vol 37 (4) ◽  
pp. 541-558 ◽  
Author(s):  
Ejgil Bojesen ◽  
Hans Degn
Keyword(s):  
Isotope Dilution ◽  
Half Life ◽  
Acid Anhydride ◽  
Hydroxyl Group ◽  
Aldosterone Level ◽  
Peripheral Plasma ◽  
Derivative Method ◽  
Double Isotope

ABSTRACT The details of a method for the determination of aldosterone at the level of 1 ng or more are described. A combination of the isotope derivative principle and the isotope dilution principle, using 35S and 131I labelled piodobenzenesulfonic acid anhydride (pipsan) as the labelled reagents, has been employed. A quantitative or almost quantitative and reproducible esterification of the 21-hydroxyl group of aldosterone and an 80% recovery of the steroid added to plasma can be achieved by the procedure described. The blank value has been determined by the analysis of epripheral plasma of adrenalectomized dogs and was found to be indistinguishable from zero and therefore less than 3 ng per 100 ml of plasma. In salt depleted anaesthetized dogs the aldosterone level was 20–30 ng per 100 ml. The concentration increased considerably during adrenalectomy and decreased with a »half life« of 20–30 minutes from the time this was completed.

Secretion rate of dehydroepiandrosterone and dehydroepiandrosterone sulfate in benign essential hypertension as compared to normal subjects

1970 ◽  
Vol 48 (12) ◽  
pp. 1308-1313 ◽  
Author(s):  
A. Shao ◽  
W. Nowaczynski ◽  
O. Kuchel ◽  
J. Genest
Keyword(s):  
Essential Hypertension ◽  
Isotope Dilution ◽  
Normal Subjects ◽  
Dehydroepiandrosterone Sulfate ◽  
Secretion Rate ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
Hypertensive Patients ◽  
Double Isotope ◽  
Column Chromatographic

A study of the secretion rate of dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA-S) in normal subjects and patients with benign essential hypertension by a double isotope dilution method showed a fivefold increase in the secretion rate of dehydroepiandrosterone (from 9.8 mg/day ± 3.1 mg/day S.D. to 52.0 mg/day ± 15.5 mg/day S.D.), and a sixfold increase of dehydroepiandrosterone sulfate in hypertensive patients (from 10.7 mg/day ± 2.9 mg/day S.D. to 60.7 mg/day ± 18.6 mg/day S.D.). This study was carried out following the administration of 14C-labelled DHEA and 3H-labelled DHEA-S and involved an initial column chromatographic separation of urinary DHEA-glucuronide and sulfate.

A Comparison of Double-Isotope Derivative and Radioimmunological Estimation of Plasma Aldosterone Concentration in Man

1973 ◽  
Vol 45 (3) ◽  
pp. 411-415 ◽  
Author(s):  
R. Fraser ◽  
Sheena Guest ◽  
Jessie Young
Keyword(s):  
Large Scale ◽  
Plasma Aldosterone ◽  
Plasma Aldosterone Concentration ◽  
Plasma Pool ◽  
Wide Range ◽  
Significant Difference ◽  
Derivative Method ◽  
Scale Population ◽  
Suitable Technique ◽  
Double Isotope

1. Two techniques for estimating plasma aldosterone concentration are compared by means of repeated assays of a plasma pool and also by analysis of a wide range of plasma samples. 2. No significant difference was found in the results obtained by the methods. Radioimmunoassay required only one tenth of the volume of plasma needed for the double-isotope derivative method. 3. Its rapidity and relative inexpensiveness makes radioimmunoassay at present the most suitable technique for large-scale population screening.

A species-specific double isotope dilution strategy for the accurate quantification of platinum–GG adducts in lung cells exposed to carboplatin

2017 ◽  
Vol 32 (7) ◽  
pp. 1320-1330 ◽  
Author(s):  
Susana Cuello-Nuñez ◽  
Raquel Larios ◽  
Christian Deitrich ◽  
Tamara Lekishvili ◽  
Volker Nischwitz ◽  
...  
Keyword(s):  
Measurement Uncertainty ◽  
Isotope Dilution ◽  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Species Specific ◽  
Double Isotope ◽  
Accurate Quantification

Measurement uncertainty of Pt–GG quantification in human lung cells by species-specific double IDMS.

On-line double isotope dilution laser ablation inductively coupled plasma mass spectrometry for the quantitative analysis of solid materials

2014 ◽  
Vol 851 ◽  
pp. 64-71 ◽  
Author(s):  
Beatriz Fernández ◽  
Pablo Rodríguez-González ◽  
J. Ignacio García Alonso ◽  
Julien Malherbe ◽  
Sergio García-Fonseca ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Laser Ablation ◽  
Isotope Dilution ◽  
Inductively Coupled Plasma ◽  
Solid Materials ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
On Line ◽  
Double Isotope

Quantitation of the Fe spatial distribution in biological tissue by online double isotope dilution analysis with LA-ICP-MS: a strategy for estimating measurement uncertainty

2016 ◽  
Vol 31 (1) ◽  
pp. 270-279 ◽  
Author(s):  
David N. Douglas ◽  
Jennifer O'Reilly ◽  
Ciaran O'Connor ◽  
Barry L. Sharp ◽  
Heidi Goenaga-Infante
Keyword(s):  
Spatial Distribution ◽  
Quantitative Analysis ◽  
Laser Ablation ◽  
Measurement Uncertainty ◽  
Isotope Dilution ◽  
Biological Tissue ◽  
Isotope Dilution Analysis ◽  
Icp Ms ◽  
On Line ◽  
Double Isotope

A strategy, systematically developed, is reported for the quantitative analysis of the Fe spatial distribution in biological tissue using laser ablation with ICP-MS and on-line double isotope dilution analysis.

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