COMPARATIVE STUDIES ON THE EFFECTS OF HUMAN PROLACTIN (HP) AND GROWTH HORMONE (HGH) FOLLOWING INTRAVENOUS ADMINISTRATION

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S141
Author(s):  
M. Apostolakis ◽  
P. Berle
Keyword(s):  
Growth Hormone ◽  
Intravenous Administration ◽  
Comparative Studies ◽  
Human Prolactin

THE INFLUENCE OF THE PINEAL GLAND ON NITROGEN METABOLISM

1969 ◽  
Vol 45 (2) ◽  
pp. 175-181 ◽  
Author(s):  
IOANA MILCU ◽  
LYDIA NANU-IONESCU ◽  
RODICA MARCEAN ◽  
VIOLETA IONESCU
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Pineal Gland ◽  
Nitrogen Metabolism ◽  
Intravenous Administration ◽  
Protein Metabolism ◽  
Amino Acid Mixture ◽  
Acid Mixture ◽  
Test Substance ◽  
Anabolic Action

SUMMARY The influence of the pineal gland on protein metabolism was investigated by using a test previously devised for studying the anabolic action of growth hormone. This test consists in measuring the accumulation of urea in blood after the intravenous administration of an amino acid mixture to nephrectomized rats injected with the test substance. Like growth hormone, pineal extract significantly reduced the quantity of urea formed, suggesting an anabolic action. In contrast, in pinealectomized animals, the quantity of urea formed significantly increased when compared with controls.

scholarly journals ELISA for Determination of Human Growth Hormone: Recognition of Helix 4 Epitopes

2004 ◽  
Vol 2004 (3) ◽  
pp. 143-149 ◽  
Author(s):  
Juliana F. Moura ◽  
Luiz DeLacerda ◽  
Romolo Sandrini ◽  
Fernanda M. Borba ◽  
Denise N. Castelo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Synthetic Peptide ◽  
Human Growth ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoaffinity Column ◽  
Receptor Dimerization ◽  
Human Prolactin

Human growth hormone (hGH) signal transduction initiates with a receptor dimerization in which one molecule binds to the receptor through sites 1 and 2. A sandwich enzyme-linked immunosorbent assay was developed for quantifying hGH molecules that present helix 4 from binding site 1. For this, horse anti-rhGH antibodies were eluted by an immunoaffinity column constituted by sepharose-rhGH. These antibodies were purified through a second column with synthetic peptide correspondent tohGH helix 4, immobilized to sepharose, and used as capture antibodies. Those that did not recognize synthetic peptide were used as a marker antibody. The working range was of 1.95 to 31.25 ng/mL of hGH. The intra-assay coefficient of variation (CV) was between 4.53% and 6.33%, while the interassay CV was between 6.00% and 8.27%. The recovery range was between 96.0% to 103.8%. There was no cross-reactivity with human prolactin. These features show that our assay is an efficient method for the determination of hGH.

EFFECT OF INTRAVENOUS ADMINISTRATION OF HUMAN GROWTH HORMONE ON SULPHATION FACTOR ACTIVITY IN SERUM OF HYPOPITUITARY SUBJECTS

1971 ◽  
Vol 66 (3) ◽  
pp. 491-497 ◽  
Author(s):  
Kerstin Hall
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Intravenous Administration ◽  
Human Growth ◽  
Significant Rise ◽  
Mean Difference ◽  
Factor Activity ◽  
The Mean

ABSTRACT Human growth hormone (HGH) administered as an iv injection of 2–4 mg to hypopituitary patients induced a rise in the levels of sulphation factor (SF) in serum. The low basal levels of SF were not changed during the first hour after HGH injection. Not until three hours after injection, when HGH values approached basal values, there was a significant rise in SF. The mean difference of SF at one and at three hours after HGH injection was 0.52 ± 0.11.

Pituitary-Specific Factor Binding to the Human Prolactin, Growth Hormone, and Placental Lactogen Genes

DNA ◽  
1989 ◽  
Vol 8 (3) ◽  
pp. 149-159 ◽  
Author(s):  
F.P. LEMAIGRE ◽  
B. PEERS ◽  
D.A. LAFONTAINE ◽  
M. MATHY-HARTERT ◽  
G.G. ROUSSEAU ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Placental Lactogen ◽  
Specific Factor ◽  
Factor Binding ◽  
Human Prolactin
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