A FLUORESCENT REACTION FOR MONOAMINES IN THE INSULIN PRODUCING CELLS OF THE GUINEA-PIG

1964 ◽  
Vol 45 (1) ◽  
pp. 133-138 ◽  
Author(s):  
Bengt Falck ◽  
Bo Hellman
Keyword(s):  
B Cells ◽  
Islets Of Langerhans ◽  
Silver Impregnation ◽  
Fluorescent Light ◽  
Storage Site ◽  
Insulin Producing Cells ◽  
Freeze Dried ◽  
Aldehyde Fuchsin ◽  
Fluorescent Products ◽  
Fluorescent Cells

ABSTRACT Certain catecholamines and tryptamines condense in freeze-dried tissues with formaldehyde to form intensely fluorescent products. A distinct fluorescence has recently been demonstrated in the islets of Langerhans from several species with this sensitive and specific technique. The fluorescent reaction of the islets has been studied in more detail in the guineapig. Using a combination of fluorescence microscopy with subsequent silver impregnation and granule staining on the same section it was found that the fluorescent cells were identical with the B cells. The B cells exhibited no, or only weak, fluorescence after administration of reserpine. The colour of the emitted fluorescent light suggests that the B cells contain a tryptamine rather than a catecholamine. Since the fluorescent material was distributed in fine cytoplasmic granules the amine may be associated with the aldehyde-fuchsin positive granules which are regarded as the storage site for insulin or some insulin precursor. No support was found for the previous concept of an adrenergic parenchymatous innervation of the islets of Langerhans.

THE SPECIFICITY OF THE ARGYROPHIL REACTION IN THE ISLETS OF LANGERHANS IN MAN

1961 ◽  
Vol 36 (1) ◽  
pp. 22-30 ◽  
Author(s):  
Bo Hellman ◽  
Claes Hellerström
Keyword(s):  
B Cells ◽  
Islets Of Langerhans ◽  
The Other ◽  
Human Pancreas ◽  
Paraffin Sections ◽  
Cell Counts ◽  
A Cells ◽  
Aldehyde Fuchsin ◽  
Differential Cell ◽  
Argyrophil Reaction

ABSTRACT By studying the Islets of Langerhans in man in thin Bouin fixed paraffin sections, first after impregnation with silver, and subsequently after removal of the silver, stained with Gomori's chrome-haematoxylin or aldehyde-fuchsin, it was possible to assess the specificity of the argyrophil reaction. Reports in the literature that some of the B cells were also silver impregnated could not be confirmed. On the other hand, the argyrophil reaction was not characteristic for all the A cells, since a minority of them were not blackened. In agreement with these observations, the considerably higher frequency of silver cells over A cells, previously reported in connection with comparative differential cell counts on the same human pancreas material, was shown to be only apparent.

scholarly journals CYTOCHEMICAL LOCALIZATION OF ADENYL CYCLASE ACTIVITY IN RAT ISLETS OF LANGERHANS

1972 ◽  
Vol 20 (11) ◽  
pp. 873-879 ◽  
Author(s):  
S. L. HOWELL ◽  
MARGARET WHITFIELD
Keyword(s):  
B Cells ◽  
Islets Of Langerhans ◽  
Adenosine Triphosphate ◽  
Adenyl Cyclase ◽  
Cyclase Activity ◽  
Cytochemical Localization ◽  
Adenyl Cyclase Activity ◽  
Rat Islets ◽  
Rat Islets Of Langerhans ◽  
A And B Cells

A cytochemical method has been used to investigate the localization of adenyl cyclase activity in A and B cells of isolated rat islets of Langerhans. Adenosine triphosphate was initially utilized as substrate, the pyrophosphate liberated being precipitated by lead ions at its site of production. The specificity of the method was increased by the use of adenylyl-imidodiphosphate as an alternative substrate; this adenosine triphosphate analogue was not hydrolyzed by adenosine triphosphatase but provided an effective substrate for adenyl cyclase. Adenyl cyclase activity, which was found to retain its glucagon and fluoride sensitivity in glutaraldehyde-fixed tissue, was found exclusively and almost uniformly in the plasma membranes of A and B cells. Storage granule membrane, incorporated into the plasma membrane during secretion of the granule content by exocytosis, appeared to be devoid of adenyl cyclase activity.

Hyperplasia and hypertrophy of B cells in the islets of Langerhans in hamsters infected with the 139H strain of scrapie

1994 ◽  
Vol 110 (2) ◽  
pp. 169-183 ◽  
Author(s):  
X. Ye ◽  
R.I. Carp ◽  
Y. Yu ◽  
R. Kozielski ◽  
P. Kozlowski
Keyword(s):  
B Cells ◽  
Islets Of Langerhans

DEVELOPMENT OF POLYPLOIDY IN B-CELLS OF NORMAL AND DIABETIC MICE

1979 ◽  
Vol 90 (2) ◽  
pp. 295-306 ◽  
Author(s):  
M. N. Pohl ◽  
F. J. Swartz
Keyword(s):  
B Cells ◽  
B Cell ◽  
Age Groups ◽  
Particle Size Analysis ◽  
Nuclear Volume ◽  
Size Analysis ◽  
Disease Symptoms ◽  
Aldehyde Fuchsin ◽  
The Relationship ◽  
Diabetic Syndrome

ABSTRACT This study was designed to clarify the relationship between pancreatic B-cell polyploidization and the progress of the diabetic syndrome in genetically diabetic (C57BL/Ks-db/db) and normal control mice (C57BL/KsJ) of matched age groups. Nuclear volume was confirmed to be a proper index of the polyploid class of the B-cell by correlation with Feulgen-DNA content as measured by microdensitometry. Nuclei of B-cells, identified by aldehyde fuchsin positive cytoplasmic granules, were traced by camera lucida and their volumes determined by semiautomatic particle size analysis. Six age groups were studied: 4.5, 7, 9.5, 12, 14.5 and 17 weeks. The major conclusions are: 1) The percentage of tetraploid nuclei in normal mice is consistently between 1.0 and 2.0% from 4.5 to 14.5 weeks of age and increases to approximately 3.0 % at 17 weeks of age; however, further studies are required to determined the significance of this increase; 2) in all age groups studied, percentages of polyploid nuclei are significantly greater in diabetic than in control mice; 3) the percentage of tetraploid nuclei in diabetic animals is elevated 220 % over controls at 4.5 weeks of age, remains constant until 12 weeks (while other parameters such as blood glucose level and body weight continue to rise) and increases significantly between 12 and 14.5 weeks of age. Implications of both the increased polyploidy observed at the onset of disease symptoms, and the dramatic increase occurring during the later stages of the disease, are discussed.

Diabetes is not associated with a change in the elemental composition of the pancreatic B cell in diabetic C57BL KsJ-db/db mice

1990 ◽  
Vol 10 (2) ◽  
pp. 217-223 ◽  
Author(s):  
Lisa Juntti-Berggren ◽  
Ulf Lindh ◽  
Per-Olof Berggren ◽  
Ove Berglund ◽  
Barbara J. Frankel
Keyword(s):  
B Cells ◽  
B Cell ◽  
Elemental Composition ◽  
Exocrine Pancreas ◽  
X Rays ◽  
Proton Bombardment ◽  
Pancreatic B Cell ◽  
Freeze Dried ◽  
Different Ages ◽  
Onset Of Diabetes

Freeze-dried pancreas sections from 7-, 17-and 27-week-old genetically diabetic (db/db) and normal (±/±) mice were subjected to proton bombardment and the concentrations of 15 elements in B cells and exocrine pancreas were calculated from the characteristic X-rays emitted. In the 7-week-old diabetic animals, B cells contained significantly above-normal levels of Na and S, while exocrine pancreas contained subnormal levels of Ca, and excess Mn. The B cells from the 17-week-old diabetic animals contained subnormal levels of Cu and the exocrine pancreas of the 27-week-old diabetic animals was deficient in Cd. The 7-, 17- and 27-week-old, genetically diabetic (db/db) mice were hyperglycemic, hyperinsulinemic and heavier than age-matched normal (±/±) mice. Although significant changes were found in elemental composition when comparing both B cells and exocrine pancreas at different ages, the changes were not consistent. Therefore, it appears as if the measured elemental changes were random and not related to the onset of diabetes.

Cryo-Ultramicrotomy of Islets of Langerhans

1974 ◽  
Vol 15 (3) ◽  
pp. 591-603
Author(s):  
S. L. HOWELL ◽  
MARGARET TYHURST
Keyword(s):  
Polyethylene Glycol ◽  
B Cells ◽  
Islets Of Langerhans ◽  
Structural Features ◽  
Silicotungstic Acid ◽  
Frozen Sections ◽  
Glutaraldehyde Fixation ◽  
Ultrathin Frozen Sections ◽  
Direct Immersion ◽  
A And B Cells

A procedure is described for the preparation of ultrathin frozen sections of glutaraldehyde-fixed or unfixed islets of Langerhans by cryo-ultramicrotomy. Freezing of the tissue was accomplished by direct immersion of isolated islets in liquid nitrogen. Sectioning was performed at a specimen temperature of -80 °C and a knife temperature of -40 °C, the ribbon of sections being collected on a trough containing 60 % dimethyl sulphoxide. Staining was accomplished with 4 % silicotungstic acid and sections were protected from drying artifacts by rinsing with 0.5% polyethylene glycol. Even in tissue not subjected to prior glutaraldehyde fixation, most of the structural features of A and B cells were well preserved in frozen sections, which were obtained in a number and quality which should render them suitable for ultrastructural, cytochemical or radioautographic studies.

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