THE THYMUS INVOLUTION ASSAY OF CORTICOTROPHIN
ABSTRACT The assay of corticotrophin in weanling rats, using the decrease in thymus weight as a criterion of response is described. A straight line was obtained over the range of doses of 120–1920 milliunits* when the log dose was plotted against the thymus weight per 100 g of body weight. A study of the factors affecting the assay has shown that there is no significant difference in response between males and females and that the thymus weight bears a direct relation to body weight over the range used. Multiple injections increased the sensitivity of the animals to corticotrophin but did not affect the precision of the assay. Purified corticotrophin preparations were found to have the same relative potency by the thymus involution method as by other subcutaneous injection assay methods.