A SIMPLE METHOD FOR THE MEASUREMENT OF TOTAL URINARY RADIOACTIVE STEROID METABOLITES AND ITS APPLICATION TO THE STUDY OF PERCUTANEOUS ABSORPTION

1961 ◽  
Vol 38 (2) ◽  
pp. 276-284 ◽  
Author(s):  
Robert E. Baker ◽  
Joseph W. Goldzieher
Keyword(s):  
Urinary Excretion ◽  
Postmenopausal Women ◽  
Percutaneous Absorption ◽  
Simple Method ◽  
Ovarian Status ◽  
Quantitative Procedure

ABSTRACT A simple and quantitative procedure for the extraction and measurement of total urinary radioactive steroid metabolites is described. The urinary excretion of topically applied 14C oestrone + 3H 17β-oestradiol and of 14C cortisol (11β, 17, 21-trihydroxy-pregn-4-ene-3, 20-dione) alone was studied with and without the addition of carrier oestrone, in both premenopausal and postmenopausal women. Over 80 percent of the cortisol, 7.5 to 41 percent of the oestrone, and 3.4 to 20 percent of the 17β-oestradiol metabolites appeared in the urine within 72 hours. Neither the ovarian status of the subjects nor the presence of carrier oestrone appeared to influence these results.

A COMPARISON OF TWO METHODS FOR MEASUREMENT OF ALDOSTERONE SECRETION RATE IN MAN

1966 ◽  
Vol 53 (2) ◽  
pp. 177-188 ◽  
Author(s):  
P. Lund-Johansen ◽  
T. Thorsen ◽  
K. F. Støa
Keyword(s):  
Urinary Excretion ◽  
Normal Subjects ◽  
Secretion Rate ◽  
Aldosterone Secretion ◽  
Simple Method ◽  
Mean Values ◽  
Pathological Conditions ◽  
Significant Difference ◽  
Derivative Method ◽  
The Mean

ABSTRACT A comparison has been made between (A), a relatively simple method for the measurement of aldosterone secretion rate, based on paper chromatography and direct densitometry of the aldosterone spot and (B) a more elaborate isotope derivative method. The mean secretion rate in 9 normal subjects was 112 ± 26 μg per 24 hours (method A) and 135 ± 35 μg per 24 hours (method B). The »secretion rate« in one adrenalectomized subject after the intravenous injection of 250 μg of aldosterone was 230 μg per 24 hours (method A) and 294 μg per 24 hours (method B). There was no significant difference in the mean values, and correlation between the two methods was good (r = 0.80). It is concluded that the densitometric method is suitable for clinical purposes as well as research, being more rapid and less expensive than the isotope derivative method. Method A also measures the urinary excretion of the aldosterone 3-oxo-conjugate, which is of interest in many pathological conditions. The densitometric method is obviously the less sensitive and a prerequisite for its use is an aldosterone secretion of 20—30 μg per 24 hours. Lower values are, however, rare in adults.

OOPHORECTOMY AND CORTISONE TREATMENT AS A METHOD OF ELIMINATING OESTROGEN PRODUCTION IN PATIENTS WITH BREAST CANCER

1964 ◽  
Vol 47 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Bengt H. Persson ◽  
Lars Risholm
Keyword(s):  
Breast Cancer ◽  
Urinary Excretion ◽  
Postmenopausal Women ◽  
Cancer Patients ◽  
Surgical Procedures ◽  
Breast Cancer Patients ◽  
Healthy Women ◽  
Adequate Dose ◽  
Secondary Rise ◽  
Excretion Level

ABSTRACT The urinary excretion of oestrone, oestradiol-17β, oestriol, and 17-hydroxycorticosteroids has been estimated in 55 premenopausal and 91 postmenopausal women with breast cancer at various stages of the disease before therapy. The depletion of oestrogen excretion caused by oophorectomy combined with cortisone treatment was studied in the patients with metastasising breast tumours (32 premenopausal and 56 postmenopausal subjects). Patients with disseminating breast cancer excreted significantly larger quantities of oestriol than healthy women of the same age, whether preor postmenopausal. The excretion of oestrone and oestradiol-17β was similar in cancer patients and healthy women of corresponding age. Oophorectomy reduced the oestrogen excretion not only in premenopausal subjects but also in quite a number of postmenopausal patients, particularly in those who were still excreting significant amounts of oestrogens. This decrease in the oestrogen output was transient and was followed by a compensatory increase, possibly of adrenal origin. Cortisone administered within a month after the oophorectomy at a dosage of 50 mg per day rapidly depressed the secondary rise in the oestrogen output to a level of about 4 to 5 μg/24 h irrespective of the age of the patient. This excretion level was unchanged as long as cortisone was given in an adequate dose, but increased rapidly when the cortisone treatment was discontinued. Oophorectomy combined with adequate cortisone treatment seems to offer an alternative to the extensive surgical procedures undertaken to eliminate oestrogen production in breast cancer patients.

96169787 Urinary excretion of relaxin after estradiol treatment of postmenopausal women

Maturitas ◽  
1996 ◽  
Vol 25 (2) ◽  
pp. 158 ◽  
Author(s):  
T.H. Lippert ◽  
F.P. Armbruster ◽  
H. Seeger ◽  
A.O. Mueck ◽  
M. Zwirner ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Postmenopausal Women ◽  
Estradiol Treatment

Nyctohemeral changes in bone turnover assessed by serum bone Gla-protein concentration and urinary deoxypyridinoline excretion: effects of growth and ageing

1992 ◽  
Vol 83 (3) ◽  
pp. 375-382 ◽  
Author(s):  
Richard Eastell ◽  
Patricia S. Simmons ◽  
Antony Colwell ◽  
Adel M. A. Assiri ◽  
Mary F. Burritt ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Bone Turnover ◽  
Urinary Excretion ◽  
Postmenopausal Women ◽  
Protein Concentration ◽  
Premenopausal Women ◽  
Adult Women ◽  
Bone Gla Protein ◽  
Night Time

1. To investigate whether there is a nyctohemeral rhythm in bone turnover, we measured serum bone Gla-protein (osteocalcin, an index of osteoblast activity) concentration every 2 h and urinary deoxypyridinoline (a marker of bone collagen resorption) excretion for 8 h periods in 10 pubertal girls (aged 10–14 years), 15 premenopausal women (aged 20–49 years) and 17 postmenopausal women (aged 50–75 years). 2. The serum concentration of bone Gla-protein and the urinary excretion of deoxypyridinoline were five times higher in the pubertal girls than in the premenopausal women. The urinary excretion of deoxypyridinoline in the postmenopausal women was twice that in the premenopausal women. 3. There was a nyctohemeral pattern in all age groups with mean night-time increases of 28% (P<0.001) in the urinary excretion of deoxypyridinoline and of 5% (P<0.001) in the serum bone Gla-protein concentration. 4. There also were nyctohemeral patterns in the urinary excretion of calcium (P<0.02), sodium (P<0.001) and potassium (P<0.001), with decreases at night. There was a negative correlation between the night-time changes in the urinary excretion of deoxypyridinoline and calcium, especially in adult women (P<0.01). 5. The serum level of parathyroid hormone increased with age, but this effect was only observed at night (01.00 to 07.00 hours). There was a nyctohemeral rhythm of the serum intact parathyroid hormone level at all ages, with a peak in the afternoon and night. 6. Thus, at night, there is a large increase in bone resorption and a small increase in osteoblastic activity, representing a nyctohemeral rhythm of bone turnover. Although the amplitudes of bone formation and bone resorption are greater during growth, the pattern of nyctohemeral changes present during growth continues up to the age of 75 years.

scholarly journals Urinary excretion of equol by postmenopausal women consuming soymilk fermented by probiotic bifidobacteria

2006 ◽  
Vol 61 (3) ◽  
pp. 438-441 ◽  
Author(s):  
D Tsangalis ◽  
G Wilcox ◽  
N P Shah ◽  
A E J McGill ◽  
L Stojanovska
Keyword(s):  
Urinary Excretion ◽  
Postmenopausal Women
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