STUDIES IN CASTRATED FEMALE RATS WITH OVARIAN TISSUE TRANSPLANTED IN THE SPLEEN

1954 ◽  
Vol 17 (1_Suppl) ◽  
pp. S3-S31 ◽  
Author(s):  
Stig Kullander
Keyword(s):  
Ovarian Tissue ◽  
Female Rats

scholarly journals Sphingosine 1-phosphate protects against radiation-induced ovarian injury in female rats—impact on mitochondrial-related genes

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Jiahui Zhao ◽  
Shuyun Zhang ◽  
Liesong Chen ◽  
Xiaolong Liu ◽  
Haihong Su ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Protective Effect ◽  
Mitochondrial Function ◽  
Target Genes ◽  
Ovarian Function ◽  
Ovarian Tissue ◽  
Differentially Expressed ◽  
Female Rats ◽  
Sphingosine 1 Phosphate ◽  
Radiation Induced

Abstract The toxic effects of ionizing radiation on the gonads have been widely recognized. Sphingosine 1-phosphate (S1P) has a protective effect on ovarian injury, and although it is known that mitochondria are involved in this process, the specific mechanism is not fully understood. The present study analysed the changes in the serum AMH and ovarian histology in Sprague-Dawley female rats exposed to X-ray radiation only or co-administered with S1P. The mRNA expression profile of ovarian tissue was further analysed via next-generation sequencing and bioinformatics approaches to screen out candidate mitochondria-related genes. Finally, differentially expressed target genes were verified by real-time PCR. The results showed that ionizing radiation could reduce the serum AMH level, destroy ovarian structure and decrease the number of follicles in rats, while S1P administration significantly attenuated the impairment of ovarian function. Gene ontology (GO) and KEGG pathway analysis revealed that a variety of genes related to mitochondrial function were differentially expressed, and the protective effect of S1P on mitochondria was more obvious in the acute phase 24 h after radiation. The differentially expressed mitochondrial function-related genes associated with the protective effect of S1P were UQCRH, MICU2 and GPX4, which were subsequently verified by RT-PCR. Therefore, ionizing radiation has a significant effect on ovarian function, and S1P has a protective effect on radiation-induced ovarian injury, in which mitochondria may play an important role. This study sheds new light on the mechanism of radiation-induced ovarian injury and helps develop a novel potential strategy to control it.

Effects of Hachimijiogan, Tokishakuyakusan and Keishibukuryogan on the Corpus Luteum Function and Weights of Various Organs in Vivo

1987 ◽  
Vol 15 (03n04) ◽  
pp. 99-108 ◽  
Author(s):  
Satoshi Usuki
Keyword(s):  
Corpus Luteum ◽  
Ovarian Tissue ◽  
Female Rats

The 27-day-old female rats received 20 IU PMS and 56 hours later, 40 IU hCG and were orally given with 20 or 200 μg of Hachimjiogan (TJ-7), Tokishakuyakusan (TJ-23) or Keishibukuryogan (TJ-25) daily for 3 or 5 days before sacrifice. Seven days after hCG treatment, the serum and ovarian tissue were assayed for progestins. 200 μg/day of TJ-23 significantly (p<0.05) decreased the progesterone and 17 αhydroxpogesterone levels, whereas it significantly (p<0.01) increased 20 α-hydroxpogesterone, TJ-7 and TJ-25 showed a tendency to decrease these progestins. These results suggest that TJ-23 has a luteolytic effect on the corpus luteum in vivo.

STEROID HORMONE FORMATION BY THE RAT OVARY.

1966 ◽  
Vol 51 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Bernard F. Rice ◽  
Albert Segaloff
Keyword(s):  
Steroid Hormone ◽  
Ovarian Tissue ◽  
Female Rats ◽  
Male Rats ◽  
Corpora Lutea ◽  
Castrate Male ◽  
Rat Ovary ◽  
17 Hydroxyprogesterone ◽  
Hormone Formation

ABSTRACT Ovaries were transplanted to the spleens of castrate male rats. After 120 days, slices of ovarian tissue, composed predominantly of corpora lutea, were incubated in Krebs-Ringer bicarbonate medium containing 50 μc acetate-1-14C. Radioactive steroid formation was assessed quantitatively by reverse isotope dilution. The formation of radioactive progesterone and 20α-hydroxy-pregn-4-en-3-one was established. The formation of radioactive 3β-hydroxy-pregn-5-en-20-one, androst-4-ene-3,17-dione, 17-hydroxyprogesterone, testosterone, oestrone and 17β-oestradiol could not be established. It appears that the corpus luteum of the rat, induced by endogenous gonadotrophins, forms only progestins from acetate-1-14C. Contrary to results previously obtained with ovarian tissue transplanted to female rats, radioactive steroid formation in vitro appeared to be augmented by luteinizing hormone (NIH-LH-S1) added to the incubation flasks. Administration of human chorionic gonadotrophin (200 IU/day) for 5 days prior to autopsy did not enhance acetate-1-14C incorporation in vitro.

Granulocyte Colony-Stimulating Factor Prevents Ischemia/Reperfusion-Induced Ovarian Injury in Rats: Evaluation of Histological and Biochemical Parameters

2018 ◽  
Vol 26 (10) ◽  
pp. 1389-1394 ◽  
Author(s):  
Ismet Hortu ◽  
Gokay Ozceltik ◽  
Cagdas Sahin ◽  
Levent Akman ◽  
Nuri Yildirim ◽  
...  
Keyword(s):  
Sham Group ◽  
Ischemia Reperfusion ◽  
Ovarian Tissue ◽  
Torsion Group ◽  
Female Rats ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Group 5 ◽  
Group 2 ◽  
Stimulating Factor

Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein commonly used in the field of medicine to treat neutropenia. Granulocyte colony-stimulating factor has also crucial roles in ameliorating the ischemia/reperfusion (I/R) injury in particular tissues. In this study, we aimed to investigate the protective effect of G-CSF on ovarian damage in experimental ovarian I/R injury. Thirty adult female rats were used. Rats were separated randomly into 5 groups; Group 1: sham group (abdominal wall was opened and closed surgically), Group 2: torsion group with 3-hour ischemia using vascular clips. Group 3: torsion + G-CSF group with 3-hour ischemia 30 minutes after the administration intraperitoneal (i.p.) of 100 µg/kg of G-CSF. Group 4: torsion-detorsion group with 3 hour ischemia and 3 hour reperfusion. Group 5: torsion-detorsion + G-CSF group with 3 hour ischemia followed by 100 µg/kg of G-CSF i.p. administration 30 minutes prior to 3 hour of detorsion/reperfusion. Ovarian tissue damage was scored on histopathology. Ovarian tissue malondialdehyde (MDA) was measured biochemically. In comparison with the sham group, both the torsion and torsion-detorsion groups had significantly higher scores for follicular degeneration, vascular congestion, edema, hemorrhage, and leukocyte infiltration ( P < .05). When compared group torsion-detorsion + G-CSF to group torsion-detorsion, parameters aforementioned significantly decreased in group torsion-detorsion + G-CSF ( P < .05). Granulocyte colony-stimulating factor has also decreased MDA levels notably both in the torsion + G-CSF and torsion-detorsion + G-CSF groups ( P < .05, P < .01). Our experimental study suggests that G-CSF can be a novel agent for the treatment of ovarian I/R injury.

Influence of Functioning Ovarian Tissue on the Secretion of Progesterone by the Adrenal Glands of Female Rats

Endocrinology ◽  
1971 ◽  
Vol 88 (6) ◽  
pp. 1425-1428 ◽  
Author(s):  
DELPHINE BARTOSIK ◽  
DONALD H. SZAROWSKI ◽  
DAVID J. WATSON
Keyword(s):  
Adrenal Glands ◽  
Ovarian Tissue ◽  
Female Rats

THE LEVEL OF URINARY NEUTRAL 17-KETOSTEROIDS IN FEMALE RATS

1960 ◽  
Vol XXXIV (III) ◽  
pp. 353-358
Author(s):  
Stig Kullander
Keyword(s):  
Ovarian Tissue ◽  
Female Rats ◽  
Adrenal Weight ◽  
Normal Female ◽  
List Type ◽  
Ovarian Tumours

ABSTRACT The excretion of 17-ketosteroids in the urine was studied in female rats aged 6 months, 1 year and 2 years. Some of the animals were intact, some were spayed at 3 weeks whilst in some of the spayed animals, ovarian tissue was autotransplanted to the spleen at the time of the operation (ovarian tumours form in this ovarian tissue after 6 months to 1 year). Normal female rats excreted about 30 μg neutral 17-ketosteroids in the urine per day independently of age (6 months to 2 years). The adrenal weight was smaller in spayed, female rats and the excretion of neutral 17-ketosteroids was less than in intact animals. Spayed female rats with ovarian tumours in the spleen excreted relatively large amount of 17-ketosteroids, the amount appearing to vary with the size of the ovarian tumours in the spleen.

Effects of Raloxifeneon on Ovarian Tissue of Mature Female Rats

2014 ◽  
Vol 11 (2) ◽  
pp. 1013-1017
Author(s):  
Ebadi Zahra ◽  
Kargar Jahromi Hossein ◽  
Abedi Hassan Ali ◽  
Farzam Mohsen ◽  
Farzam Mohammad
Keyword(s):  
Ovarian Tissue ◽  
Mature Female ◽  
Female Rats

Hachimijiogan and Tokishakuyakusan Enhance Deoxyribonucleic Acid α-Nucleitidyltransferase Activity in Relationship to DNA Synthesis by Ovarian Follicles via A Cyclic AMP System

1988 ◽  
Vol 16 (01n02) ◽  
pp. 37-46 ◽  
Author(s):  
Satoshi Usuki
Keyword(s):  
Cyclic Amp ◽  
Dna Synthesis ◽  
Deoxyribonucleic Acid ◽  
Ovarian Tissue ◽  
Ovarian Follicles ◽  
Female Rats ◽  
Amp System ◽  
Cyclic Amp Accumulation

The ovaries resected from twenty-nine day old female rats primed with 10 IU of PMS for 48 hours were incubated for 120 minutes with 2-20 μg/ml of Hachimijogan (HZ) and Tokishakuyakusan (TS). The animals in another group were injected intravenously with 20 μg of HZ and TS 48 hours after PMS injection, and 20 minutes later decapitated. Deoxyribonucleic acid (DNA) α-nucleotidyltransferase activity and cyclic AMP accumulation in ovarian tissue were significantly increased by HZ and TS. These results suggest that HZ and TS enhance DNA α-nucleotidyltransferase activity via cyclic AMP by ovarian follicles.

scholarly journals Effects of Aerobic Exercise on Rats with Hyperandrogenic Polycystic Ovarian Syndrome

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Na Li ◽  
Chenghao Yang ◽  
Huiyu Xie ◽  
Yinghong Liu ◽  
Yuanpeng Liao
Keyword(s):  
Aerobic Exercise ◽  
Polycystic Ovarian Syndrome ◽  
Ovarian Tissue ◽  
Female Rats ◽  
Leptin Resistance ◽  
Control Group ◽  
Low Grade ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Ovarian Syndrome

Hyperandrogenism is a key pathologic characteristic of polycystic ovarian syndrome (PCOS), and exercise can alleviate the accompanying inflammation and decrease the high androgen levels, but the mechanism is still unclear, so the purpose of this study is to explore the pathophysiologic characteristics of hyperandrogenic PCOS and the mechanism underlying its amelioration with aerobic exercise. Thirty-two female rats were randomly allocated to a normal control group (NC, n = 8), exercise control group (EC, n = 8), PCOS group (PC, n = 8), and PCOS plus exercise group (PE, n = 8). The PC and PE groups were injected with a dehydroepiandrosterone (DHEA) solution to induce the hyperandrogenic PCOS rat model. The EC and PE groups underwent a Masashi swimming protocol (120 min per session, 6 days/week, for 15 days). Results indicated that the concentrations of leptin (LP) in the EC group were significantly lower than those in the NC group ( p < 0.05 ). Compared with the NC group, the levels of testosterone (T), estradiol (E2), follicle-stimulating hormone (FSH), LP, anti-Müllerian hormone (AMH), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and free fatty acids (FFA) were all significantly augmented in the PC group (all p < 0.05 ). In addition, compared with the NC group, the levels of adiponectin (ADP) were significantly decreased ( p < 0.05 ), and the expression of aromatase cytochrome P450 (P450arom) in ovarian tissue was significantly elevated in the PC group ( p < 0.05 ). The levels of T, FSH, LP, and FFA were also significantly increased in the PE group ( p < 0.05 ). Compared with the PC group, the levels of T and LP in the PE group were significantly diminished ( p < 0.05 ), and the levels of ADP were significantly increased in the PE group ( p < 0.05 ). T was positively correlated with E2, FSH, AMH, LP, TNF-α, IL-6, and FFA levels, while ADP was negatively correlated with LP and E2. These results showed that hyperandrogenism, chronic low-grade inflammation, and leptin resistance may interact to influence the occurrence and development of PCOS. Aerobic exercise can alleviate the internal inflammation by relieving leptin resistance and may mitigate the sex hormone disorder and hyperandrogenism in rats with PCOS by affecting the hypothalamic-pituitary-ovarian axis.

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