scholarly journals The Overexpression of Epidermal Growth Factor (EGF) in HaCaT Cells Promotes the Proliferation, Migration, Invasion and Transdifferentiation to Epidermal Stem Cell Immunophenotyping of Adipose-Derived Stem Cells (ADSCs)

2020 ◽  
Vol 13 (1) ◽  
pp. 93-103 ◽  
Author(s):  
Yueping Mao ◽  
Jianchi Ma ◽  
Yue Xia ◽  
Xiaoyuan Xie
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Hacat Cells ◽  
Adipose Derived Stem Cells ◽  
Epidermal Stem Cell ◽  
Epidermal Growth

scholarly journals In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor

2018 ◽  
Vol 24 ◽  
pp. 4254-4261 ◽  
Author(s):  
Shangliang Chen ◽  
Mingzhu Wang ◽  
Xinglu Chen ◽  
Shaolian Chen ◽  
Li Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cytokeratin 19 ◽  
Adipose Derived Stem Cells ◽  
In Vitro Expression ◽  
Epidermal Growth

Epidermal Growth Factor-like Domain 7 Promotes Hematopoietic Stem Cell Expansion and Increases Myeloid-Megakaryocytic Lineage Priming through beta3 Integrin

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 2919-2919
Author(s):  
Chiemi Nishida ◽  
Yousef Salama ◽  
Ismael Gritli ◽  
Terumasa Umemoto ◽  
Hiromitsu Nakauchi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Extracellular Matrix Protein ◽  
Hematopoietic Stem ◽  
Megakaryocytic Differentiation ◽  
Beta3 Integrin ◽  
Epidermal Growth

Abstract Interactions between stem cells and their surrounding microenvironment, or niche, are critical for the establishment and maintenance of stem-cell properties. Niche cells within the bone marrow (BM) are contacted by the hematopoietic stem cells (HSCs), which retain their stem-cell character through the direct association with these niche cells. Within the BM microenvironment, an adhesion-dependent or -independent niche system regulates HSC function. Here we show that the extracellular matrix protein epidermal growth factor-like domain protein 7 (Egfl7) induced HSCs to enter the cell cycle and to undergo myelo-megakaryocytic differentiation both under steady state conditions and after myelosuppression in vivo. Mechanistically, we show that Egfl7 binds to the beta3 integrin expressed on HSCs, thereby activating the Akt pathway in HSCs. In beta3-/- mice, Egfl7-mediated HSC expansion, cell cycle progression and myelo-megakaryocytic differentiation did not occur. We propose that the ECM protein Egfl7 recruits dormant HSCs into active cell cycle, and can govern stress-induced hematopoiesis by beta3 integrin-dependent anchoring to the stem cell niche. Disclosures No relevant conflicts of interest to declare.

scholarly journals Hemangiosarcoma and its cancer stem cell subpopulation are effectively killed by a toxin targeted through epidermal growth factor and urokinase receptors

2013 ◽  
Vol 133 (8) ◽  
pp. 1936-1944 ◽  
Author(s):  
Jill T. Schappa ◽  
Aric M. Frantz ◽  
Brandi H. Gorden ◽  
Erin B. Dickerson ◽  
Daniel A. Vallera ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cancer Stem Cell ◽  
Cell Subpopulation ◽  
Epidermal Growth

scholarly journals CG6015 controls spermatogonia transit-amplifying divisions by epidermal growth factor receptor signaling in Drosophila testes

2021 ◽  
Vol 12 (5) ◽  
Author(s):  
Jun Yu ◽  
Qianwen Zheng ◽  
Zhiran Li ◽  
Yunhao Wu ◽  
Yangbo Fu ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Stem Cell ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptor ◽  
Gene Set Enrichment Analysis ◽  
Germline Stem Cell ◽  
Egfr Signaling ◽  
Egfr Signaling Pathway ◽  
Epidermal Growth

AbstractSpermatogonia transit-amplifying (TA) divisions are crucial for the differentiation of germline stem cell daughters. However, the underlying mechanism is largely unknown. In the present study, we demonstrated that CG6015 was essential for spermatogonia TA-divisions and elongated spermatozoon development in Drosophila melanogaster. Spermatogonia deficient in CG6015 inhibited germline differentiation leading to the accumulation of undifferentiated cell populations. Transcriptome profiling using RNA sequencing indicated that CG6015 was involved in spermatogenesis, spermatid differentiation, and metabolic processes. Gene Set Enrichment Analysis (GSEA) revealed the relationship between CG6015 and the epidermal growth factor receptor (EGFR) signaling pathway. Unexpectedly, we discovered that phosphorylated extracellular regulated kinase (dpERK) signals were activated in germline stem cell (GSC)-like cells after reduction of CG6015 in spermatogonia. Moreover, Downstream of raf1 (Dsor1), a key downstream target of EGFR, mimicked the phenotype of CG6015, and germline dpERK signals were activated in spermatogonia of Dsor1 RNAi testes. Together, these findings revealed a potential regulatory mechanism of CG6015 via EGFR signaling during spermatogonia TA-divisions in Drosophila testes.

scholarly journals The p38 signaling pathway mediates quiescence of glioma stem cells by regulating epidermal growth factor receptor trafficking

Oncotarget ◽  
2017 ◽  
Vol 8 (20) ◽  
pp. 33316-33328 ◽  
Author(s):  
Akio Soeda ◽  
Justin Lathia ◽  
Brian J. Williams ◽  
Qiulian Wu ◽  
Joseph Gallagher ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Signaling Pathway ◽  
Growth Factor Receptor ◽  
Receptor Trafficking ◽  
Glioma Stem Cells ◽  
Epidermal Growth

scholarly journals Use of adipose-derived stem cells in lymphatic tissue engineering and regeneration

2021 ◽  
Vol 48 (5) ◽  
pp. 559-567
Author(s):  
Antonio Jorge Forte ◽  
Daniel Boczar ◽  
Rachel Sarabia-Estrada ◽  
Maria T. Huayllani ◽  
Francisco R. Avila ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Systematic Review ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Cochrane Central Register ◽  
Adipose Derived Stem Cells ◽  
Lymphatic Tissue ◽  
Factor C

The potential to differentiate into different cell lines, added to the easy and cost-effective method of extraction, makes adipose-derived stem cells (ADSCs) an object of interest in lymphedema treatment. Our study’s goal was to conduct a comprehensive systematic review of the use of ADSCs in lymphatic tissue engineering and regeneration. On July 23, 2019, using PubMed/MEDLINE, Cochrane Clinical Answers, Cochrane Central Register of Controlled Trials, and Embase databases, we conducted a systematic review of published literature on the use of ADSCs in lymphatic tissue engineering and regeneration. There were no language or time frame limitations, and the following search strategy was applied: ((Adipose stem cell) OR Adipose-derived stem cell)) AND ((Lymphedema) OR Breast Cancer Lymphedema). Only original research manuscripts were included. Fourteen studies fulfilled the inclusion criteria. Eleven studies were experimental (in vitro or in vivo in animals), and only three were clinical. Publications on the topic demonstrated that ADSCs promote lymphangiogenesis, and its effect could be enhanced by modulation of vascular endothelial growth factor-C, interleukin-7, prospero homeobox protein 1, and transforming growth factor-β1. Pilot clinical studies included 11 patients with breast cancer-related lymphedema, and no significant side effects were present at 12-month follow-up. Literature on the use of ADSCs in lymphatic tissue engineering and regeneration demonstrated promising data. Clinical evidence is still in its infancy, but the scientific community agrees that ADSCs can be useful in regenerative lymphangiogenesis. Data collected in this review indicate that unprecedented advances in lymphedema treatment can be anticipated in the upcoming years.

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