Urinary cystatin C as a specific marker of tubular dysfunction

2006 ◽  
Vol 44 (3) ◽  
Author(s):  
Marc Conti ◽  
Stéphane Moutereau ◽  
Mokhtar Zater ◽  
Karim Lallali ◽  
Antoine Durrbach ◽  
...  
Keyword(s):  
Cystatin C ◽  
Specific Marker ◽  
Tubular Dysfunction ◽  
Urinary Cystatin C

Abstract

scholarly journals Urinary Cystatin C as a Marker of Tubular Dysfunction

2007 ◽  
Vol 26 (2) ◽  
pp. 98-102 ◽  
Author(s):  
Zoran Mijušković ◽  
Đorđe Maksić ◽  
Rajko Hrvačević ◽  
Marina Vučelić ◽  
Vesna Subota ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Cystatin C ◽  
Statistical Significance ◽  
Circadian Variation ◽  
Control Group ◽  
Tubular Dysfunction ◽  
Kidney Glomerulus ◽  
Patient Groups ◽  
Urinary Cystatin C

Urinary Cystatin C as a Marker of Tubular DysfunctionCystatin C (CysC) is a nonglycosylated 13 KD protein that belongs to the type II cystatin gene family. It is a strong inhibitor of cysteine proteinases, freely filtered by the kidney glomerulus and reabsorbed by the tubulus, where it is almost totally catabolized. Remainder of the nonmetabolized CysC is eliminated in urine and may represent a useful marker of tubular dysfunction. The aim of the study was to confirm the clinical importance of the quantitative determination of CysC by an automated immunonephelometric method (DADE Behring). Two groups of patients were examined: one with glomerular (GD, n=36) and one with tubular dysfunction (TD, n=31), and compared with the control group (CG, n=31) of healthy males and females from laboratory personnel (n=11) and patients on routine systematic examination (n=20), from 25 to 58 years old. The patient groups were categorised according to the urine analysis of total proteins, creatinine and adequate proteins electrophoretic panel. CysC concentration in CG was in the range of 0.02-0.15 mg/L; 0.01-0.48 mg/L and 0.25-18 mg/L in GD and TD groups respectively. Values of means ± SD for patient groups (GD=0.11 ± 0.14; TD=3.92 ± 3.75 mg/L) showed statistical significance (p<0.001) in the TD group in relation to GD and CG groups. It confirms that quantitative determination of CysC in one urine portion, with a fast laboratory method, might be a useful marker of tubular dysfunction, especially in emergency situations, taking into account that there is no interference of circadian variation on its concentration.

Analytical performances of PENIA and PETIA urinary cystatin C determination allow tubular injury investigation

2018 ◽  
Vol 56 (2) ◽  
pp. 228-231
Author(s):  
Anne-Sophie Bargnoux ◽  
Vincent Azoury ◽  
Stéphanie Badiou ◽  
Kada Klouche ◽  
Maëlle Plawecki ◽  
...  
Keyword(s):  
Cystatin C ◽  
Limit Of Detection ◽  
Acute Injury ◽  
Tubular Dysfunction ◽  
Regression Equations ◽  
Hiv Patients ◽  
Limit Of Quantification ◽  
Coefficients Of Variations ◽  
Urinary Cystatin C

Background The study was designed to evaluate the analytical performances of two ERM-DA471/IFCC traceable cystatin C (CysC) reagents available on the market for urinary CysC (u-CysC) quantification. In addition, clinical relevance was assessed by measuring u-CysC in healthy controls and in patients with tubular dysfunction. Methods CysC in urine was measured by a particle-enhanced nephelometric immunoassay using Siemens reagents and by a particle-enhanced turbidimetric immunoassay using DiaSys reagents. Imprecision, linearity, limit of detection and limit of quantification were evaluated according to CLSI recommendations. The two methods were tested on 150 urinary samples from 50 healthy subjects, 50 HIV patients with tubular dysfunction and 50 patients who developed acute kidney acute injury. Results Within-laboratory coefficients of variations were below 4%. The lower limit of quantification of the assay was found at 0.043 and 0.046 mg/L for DiaSys and Siemens, respectively. The following Passing-Bablok regression equations were obtained: DiaSys = 0.99 Siemens + 0.00. Using Bland-Altman analysis, the mean bias was –0.004 mg/L on the analytical range between 0.02 and 1 mg/L. Median u-CysC in 50 HIV patients with tubular dysfunction and in 50 patients with AKI was higher than in control subjects. Conclusions Both Siemens and DiaSys reagents demonstrated reliable and reproducible performances allowing easy determination of u-CysC on automated platforms in clinical practice with potential interest for the detection of tubular dysfunction.

Serial Quantification of Urinary Protein Biomarkers to Predict Drug-induced Acute Kidney Injury

2019 ◽  
Vol 20 (8) ◽  
pp. 656-664 ◽  
Author(s):  
Yi Da ◽  
K. Akalya ◽  
Tanusya Murali ◽  
Anantharaman Vathsala ◽  
Chuen-Seng Tan ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Cystatin C ◽  
Calcineurin Inhibitors ◽  
Kidney Injury ◽  
Tubular Dysfunction ◽  
Renal Tubular Dysfunction ◽  
Protein Biomarkers ◽  
Drug Induced ◽  
Beta 2 ◽  
Beta 2 Microglobulin

Background: : Drug-induced Acute Kidney Injury (AKI) develops in 10-15% of patients who receive nephrotoxic medications. Urinary biomarkers of renal tubular dysfunction may detect nephrotoxicity early and predict AKI. Methods:: We prospectively studied patients who received aminoglycosides, vancomycin, amphotericin, or calcineurin inhibitors, and collected their serial urine while on therapy. Patients who developed drug-induced AKI (fulfilling KDIGO criteria) were matched with non-AKI controls in a 1:2 ratio. Their urine samples were batch-analyzed at time-intervals leading up to AKI onset; the latter benchmarked against the final day of nephrotoxic therapy in non- AKI controls. Biomarkers examined include clusterin, beta-2-microglobulin, KIM1, MCP1, cystatin-C, trefoil-factor- 3, NGAL, interleukin-18, GST-Pi, calbindin, and osteopontin; biomarkers were normalized with corresponding urine creatinine. Results:: Nine of 84 (11%) patients developed drug-induced AKI. Biomarkers from 7 AKI cases with pre-AKI samples were compared with those from 14 non-AKI controls. Corresponding mean ages were 55(±17) and 52(±16) years; baseline eGFR were 99(±21) and 101(±24) mL/min/1.73m2 (all p=NS). Most biomarker levels peaked before the onset of AKI. Median levels of 5 biomarkers were significantly higher in AKI cases than controls at 1-3 days before AKI onset (all µg/mmol): clusterin [58(8-411) versus 7(3-17)], beta-2-microglobulin [1632(913-3823) versus 253(61-791)], KIM1 [0.16(0.13-0.76) versus 0.07(0.05-0.15)], MCP1 [0.40(0.16-1.90) versus 0.07(0.04-0.17)], and cystatin-C [33(27-2990) versus 11(7-19)], all p<0.05; their AUROC for AKI prediction were >0.80 (confidence intervals >0.50), with average accuracy highest for clusterin (86%), followed by beta-2-microglobulin, cystatin-C, MCP1, and KIM1 (57%) after cross-validation. Conclusion: : Serial surveillance of these biomarkers could improve the lead time for nephrotoxicity detection by days.

scholarly journals Urinary Cystatin C and Tubular Proteinuria Predict Progression of Diabetic Nephropathy

Diabetes Care ◽  
2012 ◽  
Vol 36 (3) ◽  
pp. 656-661 ◽  
Author(s):  
S. S. Kim ◽  
S. H. Song ◽  
I. J. Kim ◽  
Y. K. Jeon ◽  
B. H. Kim ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Cystatin C ◽  
Tubular Proteinuria ◽  
Urinary Cystatin C

scholarly journals Assessment of urinary cystatin C levels in HIV-1-infected patients with preserved kidney function

2018 ◽  
Vol 17 (4) ◽  
pp. 236-242
Author(s):  
Aleksandra Szymczak ◽  
Anna Szymanek-Pasternak ◽  
Małgorzata Zalewska ◽  
Krzysztof Małyszczak ◽  
Weronika Rymer ◽  
...  
Keyword(s):  
Kidney Function ◽  
Cystatin C ◽  
Urinary Cystatin C ◽  
Hiv 1

A Simple Method to Evaluate Residual Renal Function by Spot Urinary Cystatin C-to-Creatinine Ratio in Peritoneal Dialysis Patients

2010 ◽  
Vol 30 (4) ◽  
pp. 464-467 ◽  
Author(s):  
Yoko Adachi ◽  
Akira Nishio
Keyword(s):  
Renal Function ◽  
Peritoneal Dialysis ◽  
Cystatin C ◽  
Screening Method ◽  
Residual Renal Function ◽  
Urine Collection ◽  
Creatinine Ratio ◽  
Simple Method ◽  
Spot Urine ◽  
Urinary Cystatin C

Residual renal function (RRF) is a key element in the management of chronic peritoneal dialysis (PD) patients, and 24-hour creatinine clearance (24-h Ccr) and arithmetic mean of creatinine and urea nitrogen clearances [24-h (Ccr+Curea)/2] are still standard clinical techniques for the assessment of glomerular filtration rate (GFR) to represent RRF. However, it is sometimes difficult to monitor urine collection for 24 hours, especially in outpatients, and it requires serum sampling. Therefore, we devised a new and simple method to measure RRF in prevalent PD patients. Levels of urinary cystatin C (Cys-C) and creatinine in spot urine samples [24-h (Ccr+Curea)/2] were measured in 15 stable Japanese PD patients. Although no statistical correlation was seen between Cys-C and 24-h (Ccr+Curea)/2 values, a strong correlation was found between the spot urine Cys-C-to-creatinine ratio [U (Cys-C/Cr)] and 24-h (Ccr+Curea)/2. By simple linear regression analysis, the following regression equation was derived: y = 17.0 – 6.1x, where x = log 10000*U (Cys-C/Cr) ratio and y = 24-h (Ccr+Curea)/2. Measurement of U (Cys-C/Cr) ratio does not require serum sampling or 24-hour urine collection but requires only a spot urine sample. We suggest that the U (Cys-C/Cr) ratio is a simple and easy screening method to estimate GFR in PD patients.

Myeloma progression and urinary gammaglobulin affect the urinary cystatin C to diagnose acute kidney injury in multiple myeloma

2014 ◽  
Vol 81 (05) ◽  
pp. 345-349 ◽  
Author(s):  
Jae Seok Kim ◽  
Jae Won Yang ◽  
Hyeoncheol Park ◽  
Young Sub Kim ◽  
Jun Young Lee ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Acute Kidney Injury ◽  
Cystatin C ◽  
Kidney Injury ◽  
Urinary Cystatin C

Urinary cystatin C levels as a diagnostic and prognostic biomarker in patients with acute kidney injury

Nephrology ◽  
2013 ◽  
Vol 18 (4) ◽  
pp. 256-262 ◽  
Author(s):  
Moo Yong Park ◽  
Soo Jeong Choi ◽  
Jin Kuk Kim ◽  
Seung Duk Hwang ◽  
Yong Wha Lee
Keyword(s):  
Acute Kidney Injury ◽  
Cystatin C ◽  
Prognostic Biomarker ◽  
Kidney Injury ◽  
Urinary Cystatin C
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