scholarly journals Optimization of Oil Production from Cassava Pulp and Sugarcane Bagasse using Oleaginous Yeast

2019 ◽  
Vol 35 (2) ◽  
pp. 668-677 ◽  
Author(s):  
Phakamas Rachamontree ◽  
Malinee Sriariyanun ◽  
Surapun Tepaamorndech ◽  
Issaraporn Somboonwatthanakul
Keyword(s):  
Fatty Acid ◽  
Sugarcane Bagasse ◽  
Agricultural Waste ◽  
Value Added ◽  
Oil Production ◽  
Biodiesel Production ◽  
Oil Yield ◽  
Microbial Oil ◽  
Crude Lipid ◽  
Cassava Pulp

Microbial oil is one of alternative sources for biofuel and value-added chemical production in biorefining process of lignocellulosic biomass. The bottleneck of this process is the low conversion rate of lignocellulose biomass to the target product. This research aims to optimize microbial oil production as raw material for biorefining process from agricultural waste by using Plackett–Burman (PB) design and Response Surface Methodology (RSM). Acid-hydrolysates of cassava pulp and sugarcane bagasse were converted to bio-oil by fermentation activities of two oleaginous yeasts, designated as MSU2 and Ka28. The fermentation parameters were screened by PB design to identify their impacts on oil yield, and the condition of each parameter was optimized by RSM to maximized oil yield. The predicted optimal condition obtained from RSM provided the highest lipid yield at 34.56 and 21.85% from hydrolysates of cassava pulp and sugarcane bagasse, respectively. Then, fatty acid compositions in oil were analyzed by GCMS, and our results demonstrated that palmitic acid and oleic acid were the major constituents at 72.95 and 195.01 mg/g-crude lipid, respectively. These fatty acid distribution profiles were suitable for application in biodiesel production and suggested the utilization of agricultural waste in biorefining process.

scholarly journals Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Pratik Prashant Pawar ◽  
Annamma Anil Odaneth ◽  
Rajeshkumar Natwarlal Vadgama ◽  
Arvind Mallinath Lali
Keyword(s):  
Yarrowia Lipolytica ◽  
Oil Recovery ◽  
Oleaginous Yeast ◽  
Fermentation Broth ◽  
Continuous Production ◽  
Oil Production ◽  
Biofuel Production ◽  
Oil Yield ◽  
Microbial Oil

Abstract Background Recent trends in bioprocessing have underlined the significance of lignocellulosic biomass conversions for biofuel production. These conversions demand at least 90% energy upgradation of cellulosic sugars to generate renewable drop-in biofuel precursors (Heff/C ~ 2). Chemical methods fail to achieve this without substantial loss of carbon; whereas, oleaginous biological systems propose a greener upgradation route by producing oil from sugars with 30% theoretical yields. However, these oleaginous systems cannot compete with the commercial volumes of vegetable oils in terms of overall oil yields and productivities. One of the significant challenges in the commercial exploitation of these microbial oils lies in the inefficient recovery of the produced oil. This issue has been addressed using highly selective oil capturing agents (OCA), which allow a concomitant microbial oil production and in situ oil recovery process. Results Adsorbent-based oil capturing agents were employed for simultaneous in situ oil recovery in the fermentative production broths. Yarrowia lipolytica, a model oleaginous yeast, was milked incessantly for oil production over 380 h in a media comprising of glucose as a sole carbon and nutrient source. This was achieved by continuous online capture of extracellular oil from the aqueous media and also the cell surface, by fluidizing the fermentation broth over an adsorbent bed of oil capturing agents (OCA). A consistent oil yield of 0.33 g per g of glucose consumed, corresponding to theoretical oil yield over glucose, was achieved using this approach. While the incorporation of the OCA increased the oil content up to 89% with complete substrate consumptions, it also caused an overall process integration. Conclusion The nondisruptive oil capture mediated by an OCA helped in accomplishing a trade-off between microbial oil production and its recovery. This strategy helped in realizing theoretically efficient sugar-to-oil bioconversions in a continuous production process. The process, therefore, endorses a sustainable production of molecular drop-in equivalents through oleaginous yeasts, representing as an absolute microbial oil factory.

Microbial Oil Production from Lignocellulosic Biomass – Recent Development and Prospect

2014 ◽  
Vol 541-542 ◽  
pp. 397-403
Author(s):  
Zhang Nan Lin ◽  
Hong Juan Liu ◽  
Zhi Qin Wang ◽  
Jia Nan Zhang
Keyword(s):  
Lignocellulosic Biomass ◽  
Large Scale ◽  
Raw Materials ◽  
Oil Production ◽  
Renewable Resource ◽  
Raw Material ◽  
Biodiesel Production ◽  
Microbial Oil ◽  
Key Issues ◽  
Application Potential

Microbial oil is one of the ideal raw materials for biodiesel production because of its rapid reproduction and less influence by the climate and season variation. However, the high cost is one of the key issues that restricted its production in a large-scale. Lignocellulosic biomass, the cheap and renewable resource, might be the best raw material for microbial oil production by oleaginous microorganisms. Recent development on the microbial oil production from lignocellulosic biomass was summarized in this paper. Furthermore, the challenges and application potential of microbial oil were prospected.

scholarly journals Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams

Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 300 ◽  
Author(s):  
Tsakona ◽  
Papadaki ◽  
Kopsahelis ◽  
Kachrimanidou ◽  
Papanikolaou ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Carbon Sources ◽  
Oil Production ◽  
Major Fatty Acid ◽  
Added Value ◽  
Microbial Oil ◽  
Microbial Lipids ◽  
Fed Batch ◽  
Batch Mode ◽  
Intracellular Content

Diversified mixed confectionery waste streams were utilized in a two-stage bioprocess to formulate a nutrient-rich fermentation media for microbial oil production. Solid-state fermentation was conducted for the production of crude enzyme consortia to be subsequently applied in hydrolytic reactions to break down starch, disaccharides, and proteins into monosaccharides, amino acids, and peptides. Crude hydrolysates were evaluated in bioconversion processes using the red yeast Rhodosporidium toruloides DSM 4444 both in batch and fed-batch mode. Under nitrogen-limiting conditions, during fed-batch cultures, the concentration of microbial lipids reached 16.6–17 g·L−1 with the intracellular content being more than 40% (w/w) in both hydrolysates applied. R. toruloides was able to metabolize mixed carbon sources without catabolite repression. The fatty acid profile of the produced lipids was altered based on the substrate employed in the bioconversion process. Microbial lipids were rich in polyunsaturated fatty acids, with oleic acid being the major fatty acid (61.7%, w/w). This study showed that mixed food side-streams could be valorized for the production of microbial oil with high unsaturation degree, pointing towards the potential to produce tailor-made lipids for specific food applications. Likewise, the proposed process conforms unequivocally to the principles of the circular economy, as the entire quantity of confectionery by-products are implemented to generate added-value compounds that will find applications in the same original industry, thus closing the loop.

Evaluating the effect of medium composition and fermentation conditions on microbial oil production by a Fusarium strain isolated from the open ocean

2017 ◽  
Vol 60 (4) ◽  
Author(s):  
Tian-Hua Zhong ◽  
Jia-Wei Zhang ◽  
Wei Xu ◽  
Liying Huang ◽  
Zhu-Hua Luo
Keyword(s):  
Cell Growth ◽  
Lipid Accumulation ◽  
Oil Production ◽  
Culture Conditions ◽  
Medium Composition ◽  
Open Ocean ◽  
Biodiesel Production ◽  
Microbial Oil ◽  
Fermentation Conditions ◽  
Oleaginous Fungus

AbstractAs an important feedstock for biodiesel production, microbial oil has attracted considerable interest in recent years. Here, we investigated the effect of medium composition and culture conditions on cell growth and lipid accumulation of an oleaginous fungus

Microbial oil production from thermophile cyanobacteria for biodiesel production

2011 ◽  
Vol 88 (11) ◽  
pp. 3632-3635 ◽  
Author(s):  
Sevgi Ertuğrul Karatay ◽  
Gönül Dönmez
Keyword(s):  
Oil Production ◽  
Biodiesel Production ◽  
Microbial Oil

Enhanced microbial oil production by activated sludge microorganisms from sugarcane bagasse hydrolyzate

2015 ◽  
Vol 78 ◽  
pp. 114-118 ◽  
Author(s):  
Andro Mondala ◽  
Rafael Hernandez ◽  
Todd French ◽  
Magan Green ◽  
Linda McFarland ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Sugarcane Bagasse ◽  
Oil Production ◽  
Microbial Oil

scholarly journals Utilization of Agricultural Waste for the Production of Xylooligosaccharides Using Response Surface Methodology and Their In Vitro Prebiotic Efficacy

2021 ◽  
Author(s):  
Nagamani Kathiresan ◽  
Lingesh Gopal ◽  
Vijay Karuppiah ◽  
Renuka Naveenethan ◽  
David Ravindran Abraham ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Sugarcane Bagasse ◽  
Agricultural Waste ◽  
Relative Yield ◽  
Value Added ◽  
A Value ◽  
Hydrolysis Of

Abstract Air pollution is a prominent problem recently faced in various parts of India due to the burning of stubbles (coconut husk, corn cob, paddy stubbles, sugarcane bagasse, etc.) which are rich in a lignocellulosic component that can be converted into a prebiotic known as Xylooliogsaccaride (XOS). They can be produced by autohydrolysis, acid hydrolysis and enzymatic hydrolysis of xylan. In the present study, Xylan was extracted from sugarcane bagasse using two alkalis (NaOH and KOH) and the yield was compared. Xylooligosaccharide produced by enzymatic hydrolysis and their factors influencing the yield were optimized using Response Surface Methodology. Xylan and Xylooligosaccharide was characterized by FTIR, NMR, XRD, TGA and ESI-MS. Xylooligosaccharides was investigated for their prebiotic potential by in vitro study. The maximum (Relative yield of 86%) yield of xylan was observed in 20% of NaOH. Xylan peaks at 3762cm− 1, 3347 cm− 1, 2917cm− 1 represents the OH and CH stretching of xylan. The main signals at 4.26 (H-1), 3.19 (H-2), 3.59 (H-3), 3.63 (H-4) and 3.98 (H-5) ppm determines the existence of xylan. The higher amount of XOS is pH 4.75, temperature 45°C, enzyme 4U/ml and for time of 16h. The spectrum of 5.0-5.40ppm and 4.30-4.60ppm represents the α anomeric and β anomeric protons in XOS. They are resistant digested and the reaching percentage to the intestine is 95% unhydrolyzed. The maximum prebiotic index was noted in L.plantarum (1.92) and L.fermentum (1.61). The highest prebiotic index and score was observed in L.plantarum (1.9) and L.fermentum (17). The maximum bacteriocin production of Enterococcus faecium against E.fecalis (13mm) and Streptococcus pyogenes (11mm). Therefore, utilization of agricultural residues for a value-added product not only shows a great impact on environmental issues but also could double the farmer’s income

scholarly journals Utilization of Agricultural Waste for the Production of Xylooligosaccharides Using Response Surface Methodology and Their In Vitro Prebiotic Efficacy

2021 ◽  
Author(s):  
Nagamani Kathiresan ◽  
Lingesh Gopal ◽  
Renuka Naveenathan ◽  
Vijay Karuppiah ◽  
David Ravindran Abraham ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Sugarcane Bagasse ◽  
Agricultural Waste ◽  
Relative Yield ◽  
Value Added ◽  
A Value ◽  
Hydrolysis Of

Abstract Air pollution is a prominent problem recently faced in various parts of India due to the burning of stubbles (coconut husk, corn cob, paddy stubbles, sugarcane bagasse, etc.) which are rich in a lignocellulosic component that can be converted into a prebiotic known as Xylooliogsaccaride (XOS). They can be produced by autohydrolysis, acid hydrolysis and enzymatic hydrolysis of xylan. In the present study, Xylan was extracted from sugarcane bagasse using two alkalis (NaOH and KOH) and the yield was compared. Xylooligosaccharide produced by enzymatic hydrolysis and their factors influencing the yield were optimized using Response Surface Methodology. Xylan and Xylooligosaccharide was characterized by FTIR, NMR, XRD, TGA and ESI-MS. Xylooligosaccharides was investigated for their prebiotic potential by in vitro study. The maximum (Relative yield of 86%) yield of xylan was observed in 20% of NaOH. Xylan peaks at 3762cm-1, 3347 cm-1, 2917cm-1 represents the OH and CH stretching of xylan. The main signals at 4.26 (H-1), 3.19 (H-2), 3.59 (H-3), 3.63 (H-4) and 3.98 (H-5) ppm determines the existence of xylan. The higher amount of XOS is pH 4.75, temperature 45°C, enzyme 4U/ml and for time of 16h. The spectrum of 5.0-5.40ppm and 4.30-4.60ppm represents the α anomeric and β anomeric protons in XOS. They are resistant digested and the reaching percentage to the intestine is 95% unhydrolyzed. The maximum prebiotic index was noted in L.plantarum (1.92) and L.fermentum (1.61). The highest prebiotic index and score was observed in L.plantarum (1.9) and L.fermentum (17). The maximum bacteriocin production of Enterococcus faecium against E.fecalis (13mm) and Streptococcus pyogenes (11mm). Therefore, utilization of agricultural residues for a value-added product not only shows a great impact on environmental issues but also could double the farmer’s income

Microbial oils as food additives: recent approaches for improving microbial oil production and its polyunsaturated fatty acid content

2016 ◽  
Vol 37 ◽  
pp. 24-35 ◽  
Author(s):  
Stamatia Bellou ◽  
Irene-Eva Triantaphyllidou ◽  
Dimitra Aggeli ◽  
Ahmed Mohammed Elazzazy ◽  
Mohammed Nabih Baeshen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Food Additives ◽  
Oil Production ◽  
Microbial Oil ◽  
Polyunsaturated Fatty Acid Content ◽  
Microbial Oils
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