Non-invasive Imaging of Tissue-Engineered Vascular Endothelium with Iron Oxide Nanoparticles

2012 ◽  
Vol 57 (SI-1 Track-G) ◽  
Author(s):  
J. Frese ◽  
L. Hrdlicka ◽  
M. E. Mertens ◽  
L. Rongen ◽  
S. Koch ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Vascular Endothelium ◽  
Oxide Nanoparticles ◽  
Non Invasive

Non-invasive Imaging of Tissue-Engineered Vascular Endothelium with Iron Oxide Nanoparticles

nano Online ◽  
2016 ◽  
Author(s):  
J. Frese ◽  
L. Hrdlicka ◽  
M. E. Mertens ◽  
L. Rongen ◽  
S. Koch ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Vascular Endothelium ◽  
Oxide Nanoparticles ◽  
Non Invasive

scholarly journals A Novel Approach for Non-Invasive Lung Imaging and Targeting Lung Immune Cells

2020 ◽  
Vol 21 (5) ◽  
pp. 1613 ◽  
Author(s):  
Amlan Chakraborty ◽  
Simon Royce ◽  
Cordelia Selomulya ◽  
Magdalena Plebanski
Keyword(s):  
Iron Oxide ◽  
Magnetic Resonance ◽  
Iron Oxide Nanoparticles ◽  
Immune Cells ◽  
Alveolar Macrophages ◽  
Lung Imaging ◽  
Oxide Nanoparticles ◽  
Super Paramagnetic Iron Oxide ◽  
Non Invasive ◽  
The Impact

Despite developments in pulmonary radiotherapy, radiation-induced lung toxicity remains a problem. More sensitive lung imaging able to increase the accuracy of diagnosis and radiotherapy may help reduce this problem. Super-paramagnetic iron oxide nanoparticles are used in imaging, but without further modification can cause unwanted toxicity and inflammation. Complex carbohydrate and polymer-based coatings have been used, but simpler compounds may provide additional benefits. Herein, we designed and generated super-paramagnetic iron oxide nanoparticles coated with the neutral natural dietary amino acid glycine (GSPIONs), to support non-invasive lung imaging and determined particle biodistribution, as well as understanding the impact of the interaction of these nanoparticles with lung immune cells. These GSPIONs were characterized to be crystalline, colloidally stable, with a size of 12 ± 5 nm and a hydrodynamic diameter of 84.19 ± 18 nm. Carbon, Hydrogen, Nitrogen (CHN) elemental analysis estimated approximately 20.2 × 103 glycine molecules present per nanoparticle. We demonstrated that it is possible to determine the biodistribution of the GSPIONs in the lung using three-dimensional (3D) ultra-short echo time magnetic resonance imaging. The GSPIONs were found to be taken up selectively by alveolar macrophages and neutrophils in the lung. In addition, the GSPIONs did not cause changes to airway resistance or induce inflammatory cytokines. Alveolar macrophages and neutrophils are critical regulators of pulmonary inflammatory diseases, including allergies, infections, asthma and chronic obstructive pulmonary disease (COPD). Therefore, pulmonary Magnetic Resonance (MR) imaging and preferential targeting of these lung resident cells by our nanoparticles offer precise imaging tools, which can be utilized to develop precision targeted radiotherapy as well as diagnostic tools for lung cancer, thereby having the potential to reduce the pulmonary complications of radiation.

scholarly journals Sensitive detection of extremely small iron oxide nanoparticles in living mice using MP2RAGE with advanced image co-registration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Joong H. Kim ◽  
Stephen Dodd ◽  
Frank Q. Ye ◽  
Andrew K. Knutsen ◽  
Duong Nguyen ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Contrast Agents ◽  
Iron Oxide Nanoparticles ◽  
Mouse Brain ◽  
Oxide Nanoparticles ◽  
Non Invasive ◽  
T1 Map ◽  
Magnetic Resonance Imaging Mri ◽  
Molecular Contrast

AbstractMagnetic resonance imaging (MRI) is a widely used non-invasive methodology for both preclinical and clinical studies. However, MRI lacks molecular specificity. Molecular contrast agents for MRI would be highly beneficial for detecting specific pathological lesions and quantitatively evaluating therapeutic efficacy in vivo. In this study, an optimized Magnetization Prepared—RApid Gradient Echo (MP-RAGE) with 2 inversion times called MP2RAGE combined with advanced image co-registration is presented as an effective non-invasive methodology to quantitatively detect T1 MR contrast agents. The optimized MP2RAGE produced high quality in vivo mouse brain T1 (or R1 = 1/T1) map with high spatial resolution, 160 × 160 × 160 µm3 voxel at 9.4 T. Test–retest signal to noise was > 20 for most voxels. Extremely small iron oxide nanoparticles (ESIONPs) having 3 nm core size and 11 nm hydrodynamic radius after polyethylene glycol (PEG) coating were intracranially injected into mouse brain and detected as a proof-of-concept. Two independent MP2RAGE MR scans were performed pre- and post-injection of ESIONPs followed by advanced image co-registration. The comparison of two T1 (or R1) maps after image co-registration provided precise and quantitative assessment of the effects of the injected ESIONPs at each voxel. The proposed MR protocol has potential for future use in the detection of T1 molecular contrast agents.

scholarly journals In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia

2017 ◽  
Author(s):  
Olivier Sandre ◽  
Coralie Genevois ◽  
Eneko Garaio ◽  
Laurent Adumeau ◽  
Stéphane Mornet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Magnetic Field ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Hyperthermia ◽  
Oxide Nanoparticles ◽  
Magnetic Iron Oxide Nanoparticles ◽  
Non Invasive ◽  
Nanoparticles Dispersions

The present work aims to demonstrate that colloidal dispersions of magnetic iron oxide nanoparticles stabilized with dextran macromolecules placed in an alternating magnetic field can not only produce heat, but also that these particles could be used in vivo for local and non-invasive deposition of a thermal dose sufficient to trigger thermo-induced gene expression. Iron oxide nanoparticles were first characterized in vitro on a bio-inspired setup, and then they were assayed in vivo using a transgenic mouse strain expressing the luciferase reporter gene under transcriptional control of a thermosensitive promoter. Iron oxide nanoparticles dispersions were applied topically on the mouse skin or injected sub-cutaneously with Matrigel™ to generate so called pseudo tumors. Temperature was monitored continuously with a feedback loop to control the power of the magnetic field generator and to avoid overheating. Thermo-induced luciferase expression was followed by bioluminescence imaging 6 hours after heating. We showed that dextran-coated magnetic iron oxide nanoparticles dispersions were able to induce in vivo mild hyperthermia compatible with thermo-induced gene expression in surrounding tissues and without impairing cell viability. These data open new therapeutic perspectives for using mild magnetic hyperthermia as non-invasive modulation of tumor microenvironment by local thermo-induced gene expression or drug release.

A short-wavelength infrared emitting multimodal probe for non-invasive visualization of phagocyte cell migration in living mice

2014 ◽  
Vol 50 (92) ◽  
pp. 14356-14359 ◽  
Author(s):  
Y. Tsukasaki ◽  
A. Komatsuzaki ◽  
Y. Mori ◽  
Q. Ma ◽  
Y. Yoshioka ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Migration ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Short Wavelength ◽  
Rhodamine 6G ◽  
Oxide Nanoparticles ◽  
Cds Quantum Dots ◽  
Non Invasive ◽  
Lymph System

For the non-invasive visualization of phagocyte cell migration in a mouse lymph system, we developed a short-wavelength infrared (SWIR) emitting multimodal probe that contains PbS/CdS quantum dots, rhodamine 6G and iron oxide nanoparticles.

scholarly journals Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2337
Author(s):  
Ralf P. Friedrich ◽  
Iwona Cicha ◽  
Christoph Alexiou
Keyword(s):  
Tissue Engineering ◽  
Iron Oxide ◽  
Regenerative Medicine ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Specific Cell ◽  
Non Invasive ◽  
Observation Methods ◽  
Therapeutic Compounds ◽  
Kidney Liver

In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

Superparamagnetic Iron Oxide Nanoparticles (SPIONs) for Diagnosis and Treatment of Breast, Ovarian and Cervical Cancers

2020 ◽  
Vol 20 (12) ◽  
pp. 942-945 ◽  
Author(s):  
Sekhar Talluri ◽  
Rama R. Malla
Keyword(s):  
Cervical Cancer ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Diagnosis And Treatment ◽  
In Vivo Studies ◽  
Oxide Nanoparticles ◽  
Therapeutic Applications ◽  
Non Invasive ◽  
Theranostic Agents

Background: The potential of Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) as theranostic agents for cancer has been investigated extensively. SPIONS can be utilized for diagnostic imaging, drug delivery as well as for therapeutic applications. SPIONS are of particular interest because of their potential for non-invasive diagnosis and non-invasive therapeutic applications. This article is a review of in vivo and clinical studies of SPIONs for diagnosis and treatment of breast, ovarian and cervical cancer. The current limitations of this technology with relation to clinical therapeutic applications and the potential to overcome these limitations are also discussed. Methods: NCBI Pubmed was searched for relevant documents by using keyword and MESH based search. The following keyword combinations were used: ‘breast cancer’ and SPION, ‘ovarian cancer’ and SPION, and ‘cervical cancer’ and SPION. The resulting list was manually scanned for the studies involving clinical and in vivo studies. Results: The 29 most relevant publications were identified and reviewed. Conclusion: Although numerous in vitro and in vivo studies have demonstrated the safety and effectiveness of the use of SPIONs for both diagnostic and therapeutic applications, there is relatively little progress towards translation to clinical applications involving breast, ovarian and cervical cancer.

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