A short-wavelength infrared emitting multimodal probe for non-invasive visualization of phagocyte cell migration in living mice

2014 ◽  
Vol 50 (92) ◽  
pp. 14356-14359 ◽  
Author(s):  
Y. Tsukasaki ◽  
A. Komatsuzaki ◽  
Y. Mori ◽  
Q. Ma ◽  
Y. Yoshioka ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Migration ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Short Wavelength ◽  
Rhodamine 6G ◽  
Oxide Nanoparticles ◽  
Cds Quantum Dots ◽  
Non Invasive ◽  
Lymph System

For the non-invasive visualization of phagocyte cell migration in a mouse lymph system, we developed a short-wavelength infrared (SWIR) emitting multimodal probe that contains PbS/CdS quantum dots, rhodamine 6G and iron oxide nanoparticles.

scholarly journals An automatable platform for genotoxicity testing of nanomaterials based on the fluorometric γ-H2AX assay reveals no genotoxicity of properly surface-shielded cadmium-based quantum dots

Nanoscale ◽  
2019 ◽  
Vol 11 (28) ◽  
pp. 13458-13468 ◽  
Author(s):  
D. Geißler ◽  
M. Wegmann ◽  
T. Jochum ◽  
V. Somma ◽  
M. Sowa ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Gold Nanoparticles ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Semiconductor Quantum Dots ◽  
Oxide Nanoparticles ◽  
Genotoxic Potential ◽  
Genotoxicity Testing

The genotoxic potential of citrate-stabilized gold nanoparticles, micellar encapsulated iron oxide nanoparticles, and cadmium-based semiconductor quantum dots with different shell compositions was tested using the automated microscope system AKLIDES.

Non-invasive Imaging of Tissue-Engineered Vascular Endothelium with Iron Oxide Nanoparticles

nano Online ◽  
2016 ◽  
Author(s):  
J. Frese ◽  
L. Hrdlicka ◽  
M. E. Mertens ◽  
L. Rongen ◽  
S. Koch ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Vascular Endothelium ◽  
Oxide Nanoparticles ◽  
Non Invasive

Non-invasive Imaging of Tissue-Engineered Vascular Endothelium with Iron Oxide Nanoparticles

2012 ◽  
Vol 57 (SI-1 Track-G) ◽  
Author(s):  
J. Frese ◽  
L. Hrdlicka ◽  
M. E. Mertens ◽  
L. Rongen ◽  
S. Koch ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Vascular Endothelium ◽  
Oxide Nanoparticles ◽  
Non Invasive

scholarly journals A Novel Approach for Non-Invasive Lung Imaging and Targeting Lung Immune Cells

2020 ◽  
Vol 21 (5) ◽  
pp. 1613 ◽  
Author(s):  
Amlan Chakraborty ◽  
Simon Royce ◽  
Cordelia Selomulya ◽  
Magdalena Plebanski
Keyword(s):  
Iron Oxide ◽  
Magnetic Resonance ◽  
Iron Oxide Nanoparticles ◽  
Immune Cells ◽  
Alveolar Macrophages ◽  
Lung Imaging ◽  
Oxide Nanoparticles ◽  
Super Paramagnetic Iron Oxide ◽  
Non Invasive ◽  
The Impact

Despite developments in pulmonary radiotherapy, radiation-induced lung toxicity remains a problem. More sensitive lung imaging able to increase the accuracy of diagnosis and radiotherapy may help reduce this problem. Super-paramagnetic iron oxide nanoparticles are used in imaging, but without further modification can cause unwanted toxicity and inflammation. Complex carbohydrate and polymer-based coatings have been used, but simpler compounds may provide additional benefits. Herein, we designed and generated super-paramagnetic iron oxide nanoparticles coated with the neutral natural dietary amino acid glycine (GSPIONs), to support non-invasive lung imaging and determined particle biodistribution, as well as understanding the impact of the interaction of these nanoparticles with lung immune cells. These GSPIONs were characterized to be crystalline, colloidally stable, with a size of 12 ± 5 nm and a hydrodynamic diameter of 84.19 ± 18 nm. Carbon, Hydrogen, Nitrogen (CHN) elemental analysis estimated approximately 20.2 × 103 glycine molecules present per nanoparticle. We demonstrated that it is possible to determine the biodistribution of the GSPIONs in the lung using three-dimensional (3D) ultra-short echo time magnetic resonance imaging. The GSPIONs were found to be taken up selectively by alveolar macrophages and neutrophils in the lung. In addition, the GSPIONs did not cause changes to airway resistance or induce inflammatory cytokines. Alveolar macrophages and neutrophils are critical regulators of pulmonary inflammatory diseases, including allergies, infections, asthma and chronic obstructive pulmonary disease (COPD). Therefore, pulmonary Magnetic Resonance (MR) imaging and preferential targeting of these lung resident cells by our nanoparticles offer precise imaging tools, which can be utilized to develop precision targeted radiotherapy as well as diagnostic tools for lung cancer, thereby having the potential to reduce the pulmonary complications of radiation.

scholarly journals Sensitive detection of extremely small iron oxide nanoparticles in living mice using MP2RAGE with advanced image co-registration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Joong H. Kim ◽  
Stephen Dodd ◽  
Frank Q. Ye ◽  
Andrew K. Knutsen ◽  
Duong Nguyen ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Contrast Agents ◽  
Iron Oxide Nanoparticles ◽  
Mouse Brain ◽  
Oxide Nanoparticles ◽  
Non Invasive ◽  
T1 Map ◽  
Magnetic Resonance Imaging Mri ◽  
Molecular Contrast

AbstractMagnetic resonance imaging (MRI) is a widely used non-invasive methodology for both preclinical and clinical studies. However, MRI lacks molecular specificity. Molecular contrast agents for MRI would be highly beneficial for detecting specific pathological lesions and quantitatively evaluating therapeutic efficacy in vivo. In this study, an optimized Magnetization Prepared—RApid Gradient Echo (MP-RAGE) with 2 inversion times called MP2RAGE combined with advanced image co-registration is presented as an effective non-invasive methodology to quantitatively detect T1 MR contrast agents. The optimized MP2RAGE produced high quality in vivo mouse brain T1 (or R1 = 1/T1) map with high spatial resolution, 160 × 160 × 160 µm3 voxel at 9.4 T. Test–retest signal to noise was > 20 for most voxels. Extremely small iron oxide nanoparticles (ESIONPs) having 3 nm core size and 11 nm hydrodynamic radius after polyethylene glycol (PEG) coating were intracranially injected into mouse brain and detected as a proof-of-concept. Two independent MP2RAGE MR scans were performed pre- and post-injection of ESIONPs followed by advanced image co-registration. The comparison of two T1 (or R1) maps after image co-registration provided precise and quantitative assessment of the effects of the injected ESIONPs at each voxel. The proposed MR protocol has potential for future use in the detection of T1 molecular contrast agents.

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