scholarly journals Calcification of Axial Skeleton and Segmentation Muscular Regeneration of Gecko’s Tail (Gekko Gecko Linnaeus, 1758)

2019 ◽  
Vol 2 ◽  
pp. 189-192
Author(s):  
Azkiya Zahrotus Syarifah ◽  
Muhammad Ja’far Luthfi ◽  
Alfiatun Wa’is Khusnul Khatimah ◽  
Aulya Nidaur Rahmah

Gecko is an animal that can carry out autotomy. Research on gecko tail autotomy has been carried out, but there are still few who research the axial skeleton that focuses on vertebrae caudales and segmentation muscular arranged, this is the background of this research. This research is expected to be data base further research and as a comparison between animals that can induce further autotomy. This study aims to determine the macrostructure and microstructure of axial axial gecko tail regenerates and determine the anatomical microstructure of muscular regeneration of gecko tail. The methods used were X-Ray, Alizarin Red S and Alcian Blue, Paraffin method with hematoxylin-eosin staining, and Mallory Triple Stain. The results showed that the color of the gecko tail regenerate was paler compared to the original tail. On observations using radiological rays and alizarin staining showed that the original tail wo uld look segmented and have a process. The original gecko tail is composed of bones, because it is red which shows perfectly calcified bone. While the gecko tail regenerate is composed of cartilage that is shaped like a long pipe glazed red because it has calcified. At the end of the tail there is also a blue color, this indicates that the gecko tail regenerate has not been completely calcified. Segmentation Muscular of the original gecko tail, when viewed longitudinally, indicates a segment that extends from one process to the skin and when viewed from the cross has only four muscle segments separated by the septum. While the gecko tail regenerate when viewed in a longitudinal manner there is no segment and when seen transversely there are 12 muscle segments seen. Muscles are composed of a collection of myotubes that form myotomes, each myotomes limited by myoseptum.

Author(s):  
Ardiana Ardiana ◽  
Muhammad Ja’far Luthfi ◽  
Nyoman Puniawati Soesilo ◽  
Laila Ainun Nisa ◽  
Risti Widyaningsih

Lizards are animals that can do autotomy. Research on the autotomy of lizard’s tail has been carried out, but few have examined about axial skeletons that focus on vertebrae caudales or arranged muscles. That is the background of this research. This research is expected to be a database for further research and as a comparison between animals that can perform other autotomies. This research intend to determine the macro and micro structures of the anatomical axial skeleton lizard’s regenerate tail and to know the micro structures of anatomy muscular segmentation lizard’s regenerate tail. The used methods are X-Ray, Alizarin Red S and Alcian Blue, Paraffin method with Hematoxylin-Eosin and Mallory Triple Strain staining. The result showed that the lizard's regenerate tail was darker than the original. On observations using X-Ray and Alizarin staining showed that the original tail is seen broad and have a Processus. The original lizard's tail is composed of bones, because it is red which shows perfectly calcified bones. While the regenerate of the lizard's tail is composed of bones in the form of long pipes which are red-colored because they have calcified from outside. Muscular segmentation of the original lizard's tail when viewed transversely has irregular segments. While the lizard's regenerate tail has 15 muscle segments. Muscles are composed of a collection of myotubes that become myotomes, each of myotomes confined by the myoseptum.


2019 ◽  
Vol 2 ◽  
pp. 137-140
Author(s):  
Putri Nofita ◽  
Muhammad Ja’far Luthfi ◽  
Reza Sukma Dewi

Turtles are reptiles armored backs hard, slow motion, appearing for the first time about 200 million years ago and relative ly unchanged for 150 million years. This study aims to determine the anatomical structure of the axial skeleton and determine the histologic structure of red-eared turtles (Trachemys scripta elegans) females. The method used, among others, Alizarin Red S staining Alcian Blue, methods of paraffin with Hematoxylin-eosin staining (HE) and Mallory acid fuchsin and used 3 adult turtles 3 years old. The results showed that vertebral caudalis red-eared turtles (Trachemys scripta elegans) females composed of true bone, vertebrae-type amphicoelous not have plains autotomy and histology red-eared turtles (Trachemys scripta elegans) females resemble histological structure other tail reptiles with some differences that only have 4 files do not have the muscle and fat tissue.


2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ernawati

Biological information, behaviour and suitable habitat of water monitor was very less in order to support its maintenance management and breeding efforts. One of important information is the information of digestive tract, particularly the information about the structure of intestine tissue of water monitor. Sample in this research was intestine organ of water monitor. The animal was anesthetized, exanguinated, and fixed in paraformaldehyde 4% by tissue perfusion method. The intestine tissue sample for histological section with paraffin method was cutted with 3-4 μm thick and coloured with hematoxylin eosin (HE). Observation were performed to the structure of intestine histology. The results was analysed descriptively and presented in figures. Monitor lizard intestine consist of small intestine and large intestine. The small intestinal wall was observed similar to jejunum and ileum. The large intestinal wall was composed of transitional ephytelia and connective tissue. However, the ephytelial layer in this tissue was composed of transitional ephytelia that similar to vesica urinaria and there are no villi.


2016 ◽  
Vol 690 ◽  
pp. 282-285
Author(s):  
Soravich Mulinta

The purpose of the study was to investigate the effects of dolomite body, frit and potassium feldspar on the properties of color slip for decorative method of color slip on earthen ware production. The characterization of raw material was analyzed by X-ray fluorescence (XRF). The experiment started with triaxial blend of dolomite body, frit and potassium feldspar in the defined ratio and then to painting of color slips on earthenware production. After that, firing specimens at 950 °C in oxidation atmosphere. Finally, the specimens were tested microstructure and physical properties. The results showed that the ratio of 50% dolomite body, 40% frit and 10% potassium feldspar were optimum properties for decorative on earthenware body. In color slip consisting of blue color added Cobalt oxide 1% ,Green colors added chromic oxide 10% , Gray color added manganese oxide 10% ,yellow color added stain 2225 10% and hazel color added ferric oxide 2 wt %.


1979 ◽  
Vol 23 ◽  
pp. 93-97 ◽  
Author(s):  
J. W. Criss

Fundamental-parameters calculations can be made on a laboratory microcomputer fo r automatic treatment of interelement absorption and enhancement effects in x-ray fluorescence analysis. A new software package, called XRF-11, uses an efficient combination of fundamental parameters and alpha factors to compensate for any lack of measured reference materials, while taking full advantage of whatever standards are available, even just pure elements. In many cases, one multi-element standard is enough for accurate analysis.The new XRF-11 software uses the same data base of absorption coefficients, fluorescence yields, etc. as the big-computer program NRLXRF, and combines theory with experiment in a consis tent way that is similar to, but more efficient than, the treatment used in NRLXRF.


Catalysts ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1150
Author(s):  
Muhammad Hamza ◽  
Ataf Ali Altaf ◽  
Samia Kausar ◽  
Shahzad Murtaza ◽  
Nasir Rasool ◽  
...  

Dye removal through photocatalytic degradation employing nanomaterials as catalysts is a growing research area. In current studies, photocatalytic alizarin red (AR) dye degradation has been investigated by designing a series of Cr based manganese oxide nanomaterials (MH1–MH5). Synthesized nanomaterials were characterized by powder X-ray diffraction, scanning electron microscopy/energy dispersive x-ray, Brunauer–Emmett–Teller, and photoluminescence techniques and were utilized for photocatalytic AR dye degradation under UV light. AR dye degradation was monitored by UV–visible spectroscopy and percent degradation was studied for the effect of time, catalyst dose, different dye concentrations, and different pH values of dye solution. All the catalysts have shown more than 80% dye degradation exhibiting good catalytic efficiencies for dye removal. The catalytic pathway was analyzed by applying the kinetic model. A pseudo second-order model was found the best fitted kinetic model indicating a chemically-rate controlled mechanism. Values of constant R2 for all the factors studied were close to unity depicting a good correlation between experimental data.


Author(s):  
Kadek Ayu Trisna Yanti ◽  
Iriani Setyawati ◽  
Ni Putu Adriani Astiti

This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.


2017 ◽  
Vol 737 ◽  
pp. 585-589 ◽  
Author(s):  
Natthapong Monarumit ◽  
Wiwat Wongkokua ◽  
Somruedee Satitkune

Sapphire, an inorganic gem-material in a variety of corundum, mainly consists of alpha-alumina (α-Al2O3) structure. The geological origins of sapphire are related to either basaltic or metamorphic rocks. The causes of the color on sapphire are some trace elements such as Cr, Fe, and Ti. It could be mentioned that Ti atoms have cooperated with Fe atoms for creating the blue color. In this study, X-ray absorption spectroscopy (XAS) technique focused on the x-ray absorption near edge structure (XANES) and the extended x-ray absorption fine structure (EXAFS) is employed to identify the oxidation state of Ti atoms and Ti-O bond length on sapphire samples. The Ti K-edge XANES and EXAFS spectra of natural sapphires were carried out using the 13-channel array germanium detector in fluorescence mode. The XANES spectra showed that the oxidation state of Ti was Ti4+ regardless of Fe content. Moreover, the Ti-O bond length on a-Al2O3 was equal to the Ti-O bond length on rutile (TiO2) analyzed from the EXAFS spectra. From these results, it could be concluded that the oxidation state of Ti atoms on natural sapphires was Ti4+ which substitutes Al3+ on the sapphire structure.


2017 ◽  
Vol 31 (23) ◽  
pp. 1750171 ◽  
Author(s):  
Y. S. M. Alajerami ◽  
K. M. Abushab ◽  
S. I. Alagha ◽  
M. H. A. Mhareb ◽  
A. Saidu ◽  
...  

The photoluminescence, optical and physical properties of sodium borate (NB) doped with different concentrations of Dy[Formula: see text] were determined and well discussed. The samples were prepared by the melt-quenching technique and characterized by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. Photoluminescence and absorption spectra of NB glasses doped with different concentrations of Dy[Formula: see text] are reported. Ten absorption bands with hypersensitive transition at 1272 nm ([Formula: see text] [Formula: see text][Formula: see text]) and two emission bands for the transitions at [Formula: see text] [Formula: see text][Formula: see text] (blue color) and [Formula: see text] [Formula: see text][Formula: see text] (yellow color) with an excitation of 330 nm have been recorded. A series of considerable physical properties (oscillator strengths, refractive index, ions concentration, polaron radius) was calculated for each dopant concentration.


2012 ◽  
Vol 67 (6) ◽  
pp. 564-572 ◽  
Author(s):  
Sandra Scharfe ◽  
Thomas F. Fässler

The synthesis of several phosphine Cu(I) complexes is reported, and their reactivity towards the solvents ethylenediamine (en) and dimethylformamide (dmf) has been investigated. CuX(PPh3) [X = Cl (1), Br (2)], CuCl(PCy3) (3) and CuCl(PiPr3)n [n = 1 (4), 2 (5)] were prepared according to adapted literature procedures. The complexes CuCl(PiPr3)n were obtained in crystalline form from a hexane solution at -70 °C and structurally characterized for n = 1 and 2. Copper halide complexes of PCy3 (3) and PiPr3 [n=1 (4)] gave colorless solutions in both en and dmf indicating the absence of Cu(II) ions. CuCl(PiPr3)2 is stable in dmf solutions. By contrast, the complexes CuX(PPh3) (X = Cl, Br) dissolve with blue color in en and with greenish-yellow color in dmf at higher temperatures under inert gas indicating a redox process affording Cu(II). At room temperature colorless crystals of CuX(PPh3)(en)2 [X = Cl (6), Br (7)] and CuX(PPh3)(dmf) [X = Cl (8), Br (9)] were isolated from the solutions and analyzed by single-crystal X-ray diffraction.


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