scholarly journals Effect of endometrial biopsy on uterine health in tropically adapted beef cattle

2017 ◽  
Author(s):  
Helbert Ramirez
Keyword(s):  
Beef Cattle ◽  
Endometrial Biopsy ◽  
Uterine Health

scholarly journals 63 EFFECT OF ENDOMETRIAL BIOPSY ON UTERINE HEALTH OF TROPICALLY ADAPTED BEEF CATTLE

2017 ◽  
Vol 29 (1) ◽  
pp. 139
Author(s):  
O. Ramirez-Garzon ◽  
N. Satake ◽  
R. E. Lyons ◽  
C. Palmieri ◽  
J. Hill ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Embryo Transfer ◽  
Transforming Growth Factor ◽  
Interleukin 1 ◽  
Transforming Growth Factor Β ◽  
Endometrial Biopsy ◽  
Bovine Embryo ◽  
Biopsy Site ◽  
Uterine Health ◽  
Quantitative Scoring

In cattle, endometrial gene expression during the first 2 weeks after fertilization is different in cattle that conceive compared with those that fail to conceive. The potential selection of animals based on endometrial marker expression before the day of embryo transfer may improve the efficiency of bovine embryo transfer. This study aimed to evaluate the morphological and molecular changes induced by endometrial biopsy in cycling tropically adapted beef cattle. In both experiments, a single biopsy was performed on the horn ipsilateral to the corpus luteum using a circular cup biopsy forceps. The uterus was collected after slaughter, and sections were taken adjacent to the biopsy site. Five heifers were not biopsied and acted as controls. In Experiment 1, presynchronised heifers (n = 10) were biopsied on Day 7 post-oestrus (B7) and then resynchronised with cloprostenol (Estromil®, 250 μg IM) to induce oestrus (O), 2 to 3 days after injection. At Day 7 post-oestrus (D7), the reproductive tracts were collected, 10 days after biopsy (B7-O-D7). In Experiment 2, presynchronised heifers (n = 7) were biopsied on Day 4 post-oestrus (B4), and reproductive tracts were collected 3 days later (B4-D7). A quantitative scoring system was used to define histological appearance of the endometrium (1 to 25; above 16 indicates endometritis). The RNA expressions of interleukin-1-β (IL-1β) and transforming growth factor-β (TGF-β) were measured by quantitative real-time PCR (qRT-PCR). Macroscopic examination showed the uterus collected from B7-O-D7 heifers exhibited a focal, well demarcated, dark red discoloration of the endometrium (4/10) with no evidence of the biopsy site found in the remaining heifers (6/10). The biopsied uteri from B4-D7 heifers revealed no macroscopic evidence of the biopsied site (4/7), serosa congestion (2/7), and one case where a severe endometrial haemorrhage was evident (1/7). Histologically, the overall mean score was not indicative of endometritis. The RNA expression showed up-regulation of both IL-1β and TGF-β in both B7-O-D7 and B4-D7 heifers compared with non-biopsied heifers and significantly higher expression of IL-1β in B4-D7 than B7-O-D7 (P = 0.031, P < 0.05). Although in most cases there was little or no evidence of damage to the endometrium within 3 to 10 days after endometrial biopsy, there was still some evidence of up-regulation of pro-inflammatory and tissue repair genes. What effect the latter has on likelihood of conception is yet to be determined.

Relationship between uterine biopsy score, endometrial infection and inflammation in the mare

2016 ◽  
Vol 44 (03) ◽  
pp. 158-163
Author(s):  
Roland Kozdrowski ◽  
Marcin Nowak ◽  
Monika Sikora ◽  
Justyna Buczkowska
Keyword(s):  
Endometrial Biopsy ◽  
Polymorphonuclear Cells ◽  
Cytological Examination ◽  
Uterine Infection ◽  
Uterine Health ◽  
Microbiological Examination ◽  
Infection And Inflammation ◽  
Subclinical Endometritis ◽  
Biopsy Score ◽  
Infectious Endometritis

Summary Objective: Endometrial biopsy score is an accepted marker of uterine health and predicted fertility, and it has been suggested that endometrial alternations are correlated with susceptibility to persistent infectious endometritis. The objective of this study was to investigate associations of endometrial biopsy score with: 1) presence of polymorphonuclear cells (PMNs) in the epithelium and stratum compactum in histopathology; 2) presence of PMNs in cytology and 3) presence of infection in microbiology. Materials and methods: The material for examination was collected from 69 mares suspected for subclinical endometritis (bred three or more times unsuccessfully in the same breeding season) and from 15 maiden mares. Samples were collected by endometrial biopsy and cytobrush technique. Results: Endometrial alterations (biopsy score IIA, IIB, III) were found in 64 of 82 mares (78%). There was an increase in PMN occurrence for grades IIA, IIB and III. When comparing grades and PMNs infiltration, we observed statistically significant differences between grades I and IIA (p = 0.222) and grades I and IIB (p = 0.042) in samples collected by endometrial biopsy. Statistically significant differences were found in microbiological examination (biopsy p = 0.036; cytobrush p = 0.189), cytological examination (biopsy p = 0.040; cytobrush p = 0.079) and PMN infiltration (p = 0.042) between mares with biopsy scores I and IIB. Furthermore, the highest percentage of infected mares was in grade IIA and IIB, and we found statistically significant differences between grades I and IIA (p = 0.043), and grades I and IIB (p = 0.036) in biopsy samples. We observed a tendency to higher prevalence of endometrial infection in mares with biopsy score IIA, IIB and III than with biopsy score I in samples collected using cytobrush technique. However, there were no statistical significant differences. Conclusion: Degenerative endometrial changes can predispose to uterine infection and inflammation. Our study shows that mares with endometrial score I are less predisposed to infection than mares with category IIA, IIB and III. Endometrial biopsy is a reliable diagnostic tool.

Breeding and productive indicators of young animals of different genotypes of beef cattle

2019 ◽  
Vol 331 (8) ◽  
pp. 36-39
Author(s):  
E. Nasanbaev ◽  
◽  
A.B. Akhmetalieva ◽  
A.E. Nugmanova ◽  
A.K. Zhumayeva ◽  
...  
Keyword(s):  
Beef Cattle

Prevalence of vesivirus in a laboratory-based set of serum samples obtained from dairy and beef cattle

2006 ◽  
Vol 228 (2) ◽  
pp. 260-260
Author(s):  
Andreas Kurth ◽  
James F. Evermann ◽  
Douglas E. Skilling ◽  
David O. Matson ◽  
Alvin W. Smith
Keyword(s):  
Beef Cattle ◽  
Serum Samples

The theoretical background for the effective development of beef raising

2020 ◽  
Vol 18 (9) ◽  
pp. 1738-1749
Author(s):  
N.L. Titov ◽  
M.M. Nizamutdinov ◽  
G.S. Klychova
Keyword(s):  
Beef Cattle ◽  
Economic Efficiency ◽  
Performance Indicators ◽  
Theoretical Basis ◽  
Theoretical Background ◽  
Theory And Practice ◽  
Cattle Breeding ◽  
Cattle Industry ◽  
Effective Development ◽  
Cattle Husbandry

Subject. This article explores the theoretical basis of the economic efficiency of production and classifies the performance figures of beef cattle husbandry. Objectives. The article aims to define a system of indicators of beef cattle husbandry economic efficiency. Methods. For the study, we used a comparative analysis. Conclusions and Relevance. To assess the economic efficiency of agriculture production, it is necessary to use a set of indicators by beef cattle breeding sub-sector. The system of economic performance indicators of production needs to be transformed into a summarizing indicator. The results of the study can be applied in the theory and practice of determining the economic efficiency of the beef cattle industry.

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