scholarly journals Cyclic AMP and Low Molecular Weight Effector(s) Present in Yeast Extract Are Involved in Pectin Lyase Production by Penicillium griseoroseum Cultured on Sucrose

1999 ◽  
Vol 76 (2) ◽  
pp. 129-142 ◽  
Author(s):  
Maria Cristina Baracat-Pereira ◽  
Jorge Luiz Cavalcante Coelho ◽  
Rosana Cristina Minussi ◽  
Virgínia Maria Chaves-Alves ◽  
Rogelio Lopes Brandio ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cyclic Amp ◽  
Yeast Extract ◽  
Pectin Lyase ◽  
Low Molecular Weight ◽  
Penicillium Griseoroseum

scholarly journals Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum

1998 ◽  
Vol 29 (4) ◽  
pp. 246-250 ◽  
Author(s):  
Rosana Cristina Minussi ◽  
Juliana Rocha Lopes Soares-Ramos ◽  
Jorge Luiz Cavalcante Coelho ◽  
Daison Olzany Silva
Keyword(s):  
Yeast Extract ◽  
Sugar Cane ◽  
Culture Media ◽  
Cellulase Activity ◽  
Dry Weight ◽  
Pectin Lyase ◽  
Production Costs ◽  
Cane Juice ◽  
Sugar Cane Juice ◽  
Penicillium Griseoroseum

The use of other inducers as substitutes for pectin was studied aiming to reduce the production costs of pectic enzymes. The effects of sugar-cane juice on the production of pectin lyase (PL) and polygalacturonase (PG) by Penicillium griseoroseum were investigated. The fungus was cultured in a mineral medium (pH 6.3) in a rotary shaker (150 rpm) for 48 h at 25oC. Culture media were supplemented with yeast extract and sucrose or sugar-cane juice. Sugar-cane juice added singly to the medium promoted higher PL activity and mycelial dry weight when compared to pectin and the use of sugar-cane juice and yeast extract yielded levels of PG activity that were similar to those obtained with sucrose-yeast extract or pectin. The results indicated that, even at low concentrations, sugar-cane juice was capable of inducing pectin lyase and polygalacturonase with no cellulase activity in P. griseoroseum.

scholarly journals Determination of ionization constants by paper electrophoresis

1981 ◽  
Vol 195 (2) ◽  
pp. 419-426 ◽  
Author(s):  
M E Tate
Keyword(s):  
Molecular Weight ◽  
Cyclic Amp ◽  
Paper Electrophoresis ◽  
Ionization Constants ◽  
Low Molecular Weight ◽  
Electrophoretic Data ◽  
Oxalate Ions

Dimensionless apparent ionization constants of charged low-molecular-weight species may be obtained from paper-electrophoretic data at 20-25 degrees C with buffers (I0.1-0.5) of measured pH (1.5-12.5) containing oxalate ions. Relative mobilities rather than absolute mobilities were measured by using glycerol and m-nitrobenzenesulphonate respectively as standards of zero and unit mobility. Application of the procedure to ionizations of adenine, adenosine, 2′-deoxyadenosine, 3′-deoxyadenosine, 3′:5′-cyclic AMP, ADP, ADP-glucose-agrocin 84 and ATP is described.

Pectin lyase production by Penicillium griseoroseum: Effect of tea extract, caffeine, yeast extract, and pectin

1996 ◽  
Vol 18 (11) ◽  
pp. 1283-1286 ◽  
Author(s):  
R. C. Minussi ◽  
J. L. C. Coelho ◽  
M. C. Baracat-Pereira ◽  
D. O. Silva
Keyword(s):  
Yeast Extract ◽  
Pectin Lyase ◽  
Penicillium Griseoroseum ◽  
Tea Extract

A high-performance oil-free backing pump for electron microscopes

1978 ◽  
Vol 36 (1) ◽  
pp. 110-111
Author(s):  
G.K.W. Balkau ◽  
E. Bez ◽  
J.L. Farrant
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Hot Spots ◽  
High Performance ◽  
Carbonaceous Material ◽  
Contamination Rate ◽  
Low Molecular Weight ◽  
Principal Source ◽  
Rotary Pumps ◽  
Electron Microscopes

The earliest account of the contamination of electron microscope specimens by the deposition of carbonaceous material during electron irradiation was published in 1947 by Watson who was then working in Canada. It was soon established that this carbonaceous material is formed from organic vapours, and it is now recognized that the principal source is the oil-sealed rotary pumps which provide the backing vacuum. It has been shown that the organic vapours consist of low molecular weight fragments of oil molecules which have been degraded at hot spots produced by friction between the vanes and the surfaces on which they slide. As satisfactory oil-free pumps are unavailable, it is standard electron microscope practice to reduce the partial pressure of organic vapours in the microscope in the vicinity of the specimen by using liquid-nitrogen cooled anti-contamination devices. Traps of this type are sufficient to reduce the contamination rate to about 0.1 Å per min, which is tolerable for many investigations.

Low-molecular-weight heparin in the prevention and treatment of thromboembolic disorders

1998 ◽  
Vol 1 (5) ◽  
pp. 166-174 ◽  
Author(s):  
Evelyn R Hermes De Santis ◽  
Betsy S Laumeister ◽  
Vidhu Bansal ◽  
Vandana Kataria ◽  
Preeti Loomba ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight Heparin ◽  
Low Molecular Weight ◽  
Prevention And Treatment
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