Pectin lyase production by Penicillium griseoroseum: Effect of tea extract, caffeine, yeast extract, and pectin

1996 ◽  
Vol 18 (11) ◽  
pp. 1283-1286 ◽  
Author(s):  
R. C. Minussi ◽  
J. L. C. Coelho ◽  
M. C. Baracat-Pereira ◽  
D. O. Silva
Keyword(s):  
Yeast Extract ◽  
Pectin Lyase ◽  
Penicillium Griseoroseum ◽  
Tea Extract

scholarly journals Cyclic AMP and Low Molecular Weight Effector(s) Present in Yeast Extract Are Involved in Pectin Lyase Production by Penicillium griseoroseum Cultured on Sucrose

1999 ◽  
Vol 76 (2) ◽  
pp. 129-142 ◽  
Author(s):  
Maria Cristina Baracat-Pereira ◽  
Jorge Luiz Cavalcante Coelho ◽  
Rosana Cristina Minussi ◽  
Virgínia Maria Chaves-Alves ◽  
Rogelio Lopes Brandio ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cyclic Amp ◽  
Yeast Extract ◽  
Pectin Lyase ◽  
Low Molecular Weight ◽  
Penicillium Griseoroseum

Tea extract as an inexpensive inducer of pectin lyase in Penicillium griseoroseum cultured on sucrose

1997 ◽  
Vol 18 (5) ◽  
pp. 308-311 ◽  
Author(s):  
M C Baracat-Pereira ◽  
R C Minussi ◽  
J L C Coelho ◽  
D O Silva
Keyword(s):  
Pectin Lyase ◽  
Penicillium Griseoroseum ◽  
Tea Extract

scholarly journals Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum

1998 ◽  
Vol 29 (4) ◽  
pp. 246-250 ◽  
Author(s):  
Rosana Cristina Minussi ◽  
Juliana Rocha Lopes Soares-Ramos ◽  
Jorge Luiz Cavalcante Coelho ◽  
Daison Olzany Silva
Keyword(s):  
Yeast Extract ◽  
Sugar Cane ◽  
Culture Media ◽  
Cellulase Activity ◽  
Dry Weight ◽  
Pectin Lyase ◽  
Production Costs ◽  
Cane Juice ◽  
Sugar Cane Juice ◽  
Penicillium Griseoroseum

The use of other inducers as substitutes for pectin was studied aiming to reduce the production costs of pectic enzymes. The effects of sugar-cane juice on the production of pectin lyase (PL) and polygalacturonase (PG) by Penicillium griseoroseum were investigated. The fungus was cultured in a mineral medium (pH 6.3) in a rotary shaker (150 rpm) for 48 h at 25oC. Culture media were supplemented with yeast extract and sucrose or sugar-cane juice. Sugar-cane juice added singly to the medium promoted higher PL activity and mycelial dry weight when compared to pectin and the use of sugar-cane juice and yeast extract yielded levels of PG activity that were similar to those obtained with sucrose-yeast extract or pectin. The results indicated that, even at low concentrations, sugar-cane juice was capable of inducing pectin lyase and polygalacturonase with no cellulase activity in P. griseoroseum.

Influence of different mixing and aeration regimens on pectin lyase production by Penicillium griseoroseum

2003 ◽  
Vol 38 (6) ◽  
pp. 849-854 ◽  
Author(s):  
R.H Piccoli-Valle ◽  
F.J.V Passos ◽  
I.V Brandi ◽  
L.A Peternelli ◽  
D.O Silva
Keyword(s):  
Pectin Lyase ◽  
Penicillium Griseoroseum

Pectin lyase production by Penicillium griseoroseum grown in sugar cane juice in repeated batch cultures

2001 ◽  
Vol 17 (5) ◽  
pp. 433-437 ◽  
Author(s):  
Roberta Hilsdorf Piccoli-Valle ◽  
Igor Viana Brandi ◽  
Daison Olzany Silva ◽  
Frederico José Vieira Passos
Keyword(s):  
Sugar Cane ◽  
Pectin Lyase ◽  
Batch Cultures ◽  
Cane Juice ◽  
Sugar Cane Juice ◽  
Penicillium Griseoroseum ◽  
Repeated Batch

scholarly journals Molecular characterization and expression profile of pectin-lyase-encoding genes from Penicillium griseoroseum

2006 ◽  
Vol 52 (11) ◽  
pp. 1070-1077 ◽  
Author(s):  
Denise S Bazzolli ◽  
Andréa O.B Ribon ◽  
Marisa V de Queiroz ◽  
Elza F de Araújo
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Carbon Sources ◽  
Pectin Lyase ◽  
Open Reading Frame ◽  
Northern Analysis ◽  
Calculated Molecular Mass ◽  
Reading Frame ◽  
Penicillium Griseoroseum ◽  
Pectin Lyases

Penicillium griseoroseum has been studied by our group because of its good pectinase production. Attempts have been done to clone pectinolytic genes, aiming to obtain pectinase-overproducing strains for industrial purposes. Here, two genes coding for pectin lyase were isolated from the P. griseoroseum genome. The plg1 gene has an open reading frame of 1341 bp coding for a putative protein of 374 amino acids with a calculated molecular mass of 40.1 kDa. The plg2 gene is characterized by an open reading frame of 1400 nucleotides and codes for a polypeptide of 383 amino acids. The plg1 gene 5′-flanking region contains putative binding sites for the transcription factors involved in regulation by ambient pH and catabolite repression. The primary structure of Plg1 and Plg2 proteins showed a relatively high homology (varying between 32.4% and 74.8%) to fungal pectin lyases characterized to date. Southern blotting analysis revealed that both genes are present as single copies in the fungus genome. Expression studies revealed a differing pattern of gene expression of plg1 and plg2 when mycelium was cultivated on medium containing different pectic components. Citric pectin followed by apple pectin were the carbon sources that best induced plg1 expression, and transcripts were detected from 24 to 76 h. The expression of the plg2 gene was monitored by reverse transcriptase – polymerase chain reaction, since Northern analysis failed to detect hybridization signals. The differential expression of these genes may provide means for the fungus to adapt to various growth conditions.Key words: pectin lyase, gene cloning, Penicillium griseoroseum, gene expression.

scholarly journals Methylxanthines as inducers of pectin lyase in Penicillium griseoroseum cultured on sucrose

1997 ◽  
Vol 24 (5) ◽  
pp. 369-372 ◽  
Author(s):  
R. C. Minussi ◽  
M. C. Baracat‐Pereira ◽  
J. L. C. Coelho ◽  
D. O. Silva
Keyword(s):  
Pectin Lyase ◽  
Penicillium Griseoroseum

scholarly journals Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer

2001 ◽  
Vol 32 (2) ◽  
Author(s):  
Roberta Hilsdorf Piccoli-Valle ◽  
Flávia Maria Lopes Passos ◽  
Frederico José Vieira Passos ◽  
Daison Olzany Silva
Keyword(s):  
Pectin Lyase ◽  
Penicillium Griseoroseum

scholarly journals Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression

2017 ◽  
Vol 48 (3) ◽  
pp. 602-606 ◽  
Author(s):  
Juliana Oliveira Lima ◽  
Jorge Fernando Pereira ◽  
Elza Fernandes de Araújo ◽  
Marisa Vieira de Queiroz
Keyword(s):  
Catabolite Repression ◽  
Pectin Lyase ◽  
Penicillium Griseoroseum
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