The Determination of Plasma Volume Using Coomassie Blue Dye as Indicator - III - A Comparison of the Values Obtained by this Method with those Obtained in the Same Individuals on the Same Day Using Evans' Blue Dye (Twenty-four Cases) and Radioiodinated Albumin (Eleven Cases)

1967 ◽  
Vol 52 (4) ◽  
pp. 525-530 ◽  
Author(s):  
WILLIAM BRADLEY MARTIN ◽  
GEORG F. KOCH
Keyword(s):  
Plasma Volume ◽  
Evans Blue ◽  
Blue Dye ◽  
Evans Blue Dye ◽  
Coomassie Blue

Determination of plasma volume in swine by the enzyme-dilution method

1987 ◽  
Vol 252 (5) ◽  
pp. R1003-R1008
Author(s):  
M. A. Holmes ◽  
R. B. Weiskopf
Keyword(s):  
Plasma Volume ◽  
Evans Blue ◽  
Dilution Method ◽  
Blue Dye ◽  
Time Activity ◽  
Evans Blue Dye ◽  
Before And After ◽  
Concentration Curves ◽  
Alt Activity

Plasma volume of six young swine was determined by simultaneous dilution in the plasma of two purified porcine enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and Evans blue dye. Ninety-minute time-activity and time-concentration curves were obtained, and the dilution spaces of each plasma indicator at the time of injection were estimated by extrapolation of data from the first 30 min after injection. Plasma volumes estimated using the two enzymes agreed closely with each other (AST, mean 50.2 ml/kg; ALT, mean 49.8 ml/kg) and were 11% smaller than those determined using Evans blue dye (mean 56.3 ml/kg). Plasma volumes calculated from the AST and ALT activities and Evans blue dye concentration in a sample drawn 5 min after injection very closely approximated those obtained by extrapolation. These values were comparable to previously published estimates of plasma volume for swine of equivalent weight. Endogenous plasma levels of AST and ALT activity were measured in samples drawn from five swine before and after a 40% blood loss. No significant change in AST or ALT activities occurred after hemorrhage.

scholarly journals Estimating plasma volume in neonatal Holstein calves fed one or two feedings of a lacteal-based colostrum replacer using Evans blue dye and hematocrit values at various time points

2015 ◽  
Vol 95 (2) ◽  
pp. 293-298 ◽  
Author(s):  
Rosemarie G. Cabral ◽  
Colleen E. Chapman ◽  
Emily J. Kent ◽  
Peter S. Erickson
Keyword(s):  
Body Weight ◽  
Plasma Volume ◽  
Evans Blue ◽  
Treatment Effects ◽  
Birth Date ◽  
Blue Dye ◽  
Time Points ◽  
Evans Blue Dye ◽  
Holstein Calves ◽  
Feeding Regimen

Cabral, R. G., Chapman, C. E., Kent, E. J. and Erickson, P. S. 2015. Estimating plasma volume in neonatal Holstein calves fed one or two feedings of a lacteal-based colostrum replacer using Evans blue dye and hematocrit values at various time points. Can. J. Anim. Sci. 95: 293–298. Twenty-eight Holstein calves were blocked by birth date and randomly assigned to one of two treatments to investigate the effect of colostrum replacer (CR) feeding regimen on plasma volume (PV). Treatments were: (1) one feeding of CR (C1; 3 L of reconstituted CR 675 g of powder providing 184.5 g of IgG at birth) or (2) two feedings of CR (C2; 2 L of reconstituted CR at birth and 1 L of reconstituted CR at 6 h). By 6 h of age, all calves had received 3 L of CR providing 184.5 g of IgG. Plasma volume was estimated at 6, 12, 18, and 24 h after birth using Evans blue dye. No treatment effects were noted at any time points (P>0.05). Mean PV for all calves regardless of treatment at 6, 12, 18, and 24 h were 78.6, 89.2, 83.9, and 90.7 mL kg−1 of body weight, respectively. Plasma volume was correlated with hematocrit (HCT), initial HCT, and treatment. Hematocrit was correlated with PV, initial HCT, and body weight. Hematocrit for 6, 12, 18 and 24 h after birth can be predicted with an initial precolostral HCT determination.

A modified evans blue dye method for determining plasma volume in the horse

1988 ◽  
Vol 8 (3) ◽  
pp. 208-212 ◽  
Author(s):  
Kenneth H. McKeever ◽  
William A. Schurg ◽  
Victor A. Convertino
Keyword(s):  
Plasma Volume ◽  
Evans Blue ◽  
Blue Dye ◽  
Evans Blue Dye

Measurement of plasma volume in pregnancy

1992 ◽  
Vol 83 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Mark A. Brown ◽  
Dina A. Mitar ◽  
Judith A. Whitworth
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Pregnant Women ◽  
Human Serum ◽  
Plasma Volume ◽  
Evans Blue ◽  
Usual Method ◽  
Blue Dye ◽  
Limits Of Agreement ◽  
Evans Blue Dye

1. Determination of the plasma volume in pregnant women is a useful research tool and may become an important clinical measurement. We used three methods to determine plasma volume using Evans Blue dye: (1) the ‘usual’ method, measuring serum absorbance at a wavelength of 610 nm, (2) a two-wavelength method, and (3) precipitation of non-albumin proteins by the addition of polyethyleneglycol before measuring serum absorbance at a wavelength of 620 nm. These were each compared with the standard 125I-human serum albumin method in 20 non-pregnant subjects. Subsequently, the polyethylene glycol method was considered the standard and the three Evans Blue dye methods were compared in 20 pregnant women. 2. In non-pregnant subjects mean plasma volumes did not differ significantly according to the method used. However, the limits of agreement with 125I-human serum albumin method were closest for the polyethyleneglycol method, for both clear and turbid sera. 3. In pregnant women, mean plasma volume values did not differ according to the Evans Blue dye method used, but the limits of agreement were significantly closer with the two-wavelength method than with the ‘usual’ method (P<0.05) largely owing to the effects of turbid sera. 4. These studies demonstrate that considerable error may occur when the Evans Blue dye concentration is determined in turbid sera by the ‘usual’ method. This can be overcome by the use of the two-wavelength method or the polyethyleneglycol method. The most accurate results will be obtained if the latter method is employed routinely to determine plasma volume in pregnant women.

Evans blue dye as a marker of albumin clearance in cultured endothelial monolayer and isolated lung

1992 ◽  
Vol 72 (3) ◽  
pp. 865-873 ◽  
Author(s):  
C. E. Patterson ◽  
R. A. Rhoades ◽  
J. G. Garcia
Keyword(s):  
Endothelial Cell ◽  
Evans Blue ◽  
Lavage Fluid ◽  
Blue Dye ◽  
Protein Transfer ◽  
Evans Blue Dye ◽  
Isolated Lung ◽  
Perfused Lung ◽  
Albumin Clearance

Determination of protein transfer across the endothelial barrier or the entire alveolar capillary membrane is critical for investigation of mechanisms leading to pulmonary edema. The purpose of this study was to evaluate Evans blue dye for determination of protein clearance across cultured bovine pulmonary artery endothelial cell monolayers and as a quantitative marker for albumin leakage to the air spaces in isolated perfused rat lungs. Evans blue dye bound tightly to albumin (EBA) as determined by lack of transfer through dialysis membranes and specific elution with albumin from a molecular exclusion column. EBA was equivalent to 125I-labeled albumin for calculation of albumin clearance rates (Calb) across intact and challenged monolayers [Calb (+ vehicle) = 0.12 microliters/min; Calb (+10 nM alpha-thrombin) = 0.47 microliters/min; Calb (+5 mg/ml trypsin) = 1.29 microliters/min]. Transfer of EBA was linear with time in both the endothelial cell monolayer model and the perfused lung. EBA was a sensitive marker for early edema in the perfused lung (before detectable weight gain) as well as for severe edema in the oxidant-injured lung (marked EBA accumulation in lavage fluid) and was a more specific marker for protein transfer than lavage fluid protein. EBA transfer is a convenient, reproducible, and accurate means to assess alterations in vascular permeability.

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