Label-free, Non-invasive Mapping of Water and Lipid Content in Tissue with Structured Shortwave-infrared Imaging (SSI)

2018 ◽  
Author(s):  
Yanyu Zhao ◽  
Matthew B. Applegate ◽  
John P. Dumas ◽  
Mark C. Pierce ◽  
Darren Roblyer
Keyword(s):  
Lipid Content ◽  
Infrared Imaging ◽  
Label Free ◽  
Non Invasive ◽  
Shortwave Infrared

scholarly journals Non-invasive monitoring of chronic liver disease via near-infrared and shortwave-infrared imaging of endogenous lipofuscin

2020 ◽  
Vol 4 (8) ◽  
pp. 801-813 ◽  
Author(s):  
Mari Saif ◽  
Wilhelmus J. Kwanten ◽  
Jessica A. Carr ◽  
Ivy X. Chen ◽  
Jessica M. Posada ◽  
...  
Keyword(s):  
Liver Disease ◽  
Chronic Liver Disease ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Chronic Liver ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
Shortwave Infrared

scholarly journals Label-free non-invasive quantitative measurement of lipid contents in individual microalgal cells using refractive index tomography

2017 ◽  
Author(s):  
JaeHwang Jung ◽  
Seong-Joo Hong ◽  
Han-Byeol Kim ◽  
Geon Kim ◽  
Moosung Lee ◽  
...  
Keyword(s):  
Refractive Index ◽  
Lipid Content ◽  
Three Dimensional ◽  
Biofuel Production ◽  
Chemical Extraction ◽  
Label Free ◽  
Non Invasive ◽  
Lipid Contents ◽  
Dry Cell Weight ◽  
Rapid Quantification

Microalgae are promising candidates for biofuel production due to their high lipid content. To facilitate utilization of the microalgae for biofuel, rapid quantification of the lipid contents in microalgae is necessary. However, conventional methods based on the chemical extraction of lipids require a time-consuming destructive extraction process. Here, we demonstrate label-free, non-invasive, rapid quantification of the lipid contents in individual micro-algal cells measuring the three-dimensional refractive index tomograms. We measure three-dimensional refractive index distributions within Nannochloropsis oculata cells and find that lipid droplets are identifiable in tomograms by their high refractive index. In addition, we alter N. oculata under nitrogen deficiency by measuring the volume, lipid weight, and dry cell weight of individual cells. Characterization of individual cells allows correlative analysis between the lipid content and size of individual cells.

scholarly journals Author Correction: Non-invasive monitoring of chronic liver disease via near-infrared and shortwave-infrared imaging of endogenous lipofuscin

2020 ◽  
Vol 4 (12) ◽  
pp. 1222-1222
Author(s):  
Mari Saif ◽  
Wilhelmus J. Kwanten ◽  
Jessica A. Carr ◽  
Ivy X. Chen ◽  
Jessica M. Posada ◽  
...  
Keyword(s):  
Liver Disease ◽  
Chronic Liver Disease ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Chronic Liver ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
Shortwave Infrared

scholarly journals Shortwave-infrared meso-patterned imaging enables label-free mapping of tissue water and lipid content

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yanyu Zhao ◽  
Anahita Pilvar ◽  
Anup Tank ◽  
Hannah Peterson ◽  
John Jiang ◽  
...  
Keyword(s):  
Blood Lipids ◽  
Imaging Modality ◽  
Label Free ◽  
Tissue Water ◽  
Absorption Bands ◽  
Non Invasive ◽  
Physiological Processes ◽  
Key Participants ◽  
Shortwave Infrared

Abstract Water and lipids are key participants in many biological processes, but there are few non-invasive methods that provide quantification of these components in vivo, and none that can isolate and quantify lipids in the blood. Here we develop a new imaging modality termed shortwave infrared meso-patterned imaging (SWIR-MPI) to provide label-free, non-contact, spatial mapping of water and lipid concentrations in tissue. The method utilizes patterned hyperspectral illumination to target chromophore absorption bands in the 900–1,300 nm wavelength range. We use SWIR-MPI to monitor clinically important physiological processes including edema, inflammation, and tumor lipid heterogeneity in preclinical models. We also show that SWIR-MPI can spatially map blood-lipids in humans, representing an example of non-invasive and contact-free measurements of in vivo blood lipids. Together, these results highlight the potential of SWIR-MPI to enable new capabilities in fundamental studies and clinical monitoring of major conditions including obesity, cancer, and cardiovascular disease.

Label-free non-invasive classification of rice seeds using optical coherence tomography assisted with deep neural network

2021 ◽  
Vol 137 ◽  
pp. 106861
Author(s):  
Deepa Joshi ◽  
Ankit Butola ◽  
Sheetal Raosaheb Kanade ◽  
Dilip K. Prasad ◽  
S.V. Amitha Mithra ◽  
...  
Keyword(s):  
Neural Network ◽  
Optical Coherence Tomography ◽  
Deep Neural Network ◽  
Optical Coherence ◽  
Label Free ◽  
Non Invasive ◽  
Rice Seeds

scholarly journals Analytical Approach for the Study of Teotihuacan Mural Paintings from the Techinantitla Complex

Minerals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 508
Author(s):  
José Luis Ruvalcaba-Sil ◽  
Luis Barba ◽  
Edgar Casanova-González ◽  
Alejandro Mitrani ◽  
Margarita Muñoz ◽  
...  
Keyword(s):  
Fiber Optics ◽  
Infrared Imaging ◽  
Blue Pigment ◽  
Large Set ◽  
Non Invasive ◽  
Mural Paintings ◽  
Interdisciplinary Research Project ◽  
Manufacturing Techniques ◽  
Materials Used ◽  
The 1960S

Techinantitla building complex, in the Amanalco neighborhood of the ancient city of Teotihuacan, is famous for the iconography and quality of the mural paintings found in this site. A significant part of this heritage has been lost due to looting. In recent years, an interdisciplinary research project was developed to study the limited patrimony that was left. As part of this study, we first employed geophysical techniques to reconstruct the architectural pattern of the compound’s remaining walls, where other paintings may still be found. Then, we applied a non-invasive methodology to characterize a large set of fragments recovered in the 1980s and to gain information on their pigments and manufacturing techniques. This methodology included False Color Infrared Imaging, X-ray Fluorescence and Fiber-Optics Reflectance Spectroscopy, and led to the identification of hematite, calcite, malachite, azurite and an unidentified blue pigment. The results were compared with a previous study performed on a set of Techinantitla mural paintings looted in the 1960s. A broader comparison with contemporary mural paintings from other Teotihuacan complexes shows good agreement in the materials used. These results may suggest a standardization in the making of Teotihuacan mural painting during the Xolapan period (350 to 550 AD).

scholarly journals Non-Invasive Identification of Nutrient Components in Grain

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3124
Author(s):  
Charles Farber ◽  
A. S. M. Faridul Islam ◽  
Endang M. Septiningsih ◽  
Michael J. Thomson ◽  
Dmitry Kurouski
Keyword(s):  
Rice Variety ◽  
Nutrient Content ◽  
Plant Diseases ◽  
Variety Identification ◽  
Successful Implementation ◽  
Label Free ◽  
Accurate Identification ◽  
Analytical Technique ◽  
Non Invasive ◽  
Digital Farming

Digital farming is a modern agricultural concept that aims to maximize the crop yield while simultaneously minimizing the environmental impact of farming. Successful implementation of digital farming requires development of sensors to detect and identify diseases and abiotic stresses in plants, as well as to probe the nutrient content of seeds and identify plant varieties. Experimental evidence of the suitability of Raman spectroscopy (RS) for confirmatory diagnostics of plant diseases was previously provided by our team and other research groups. In this study, we investigate the potential use of RS as a label-free, non-invasive and non-destructive analytical technique for the fast and accurate identification of nutrient components in the grains from 15 different rice genotypes. We demonstrate that spectroscopic analysis of intact rice seeds provides the accurate rice variety identification in ~86% of samples. These results suggest that RS can be used for fully automated, fast and accurate identification of seeds nutrient components.

scholarly journals Tethered Lipid Membranes as a Nanoscale Arrangement Towards Non-Invasive Analysis of Acute Pancreatitis

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 755
Author(s):  
Rima Budvytyte ◽  
Akvile Milasiute ◽  
Dalius Vitkus ◽  
Kestutis Strupas ◽  
Aiste Gulla ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Lipid Membranes ◽  
Electrochemical Impedance ◽  
Membrane Integrity ◽  
Electrochemical Technique ◽  
Label Free ◽  
Non Invasive ◽  
Novel Approach ◽  
Analytical System ◽  
Shock Proteins

Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.

Non-invasive label-free investigation and typing of head and neck cancers by multimodal nonlinear microscopy

2012 ◽  
Author(s):  
Tobias Meyer ◽  
Nadine Vogler ◽  
Benjamin Dietzek ◽  
Denis Akimov ◽  
Johanna Inhestern ◽  
...  
Keyword(s):  
Head And Neck ◽  
Head And Neck Cancers ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Non Invasive

Non-invasive and label-free identification of human natural killer subclasses by biophysical single-cell features in microfluidic flow

Lab on a Chip ◽  
2021 ◽  
Author(s):  
David Dannhauser ◽  
Domenico Rossi ◽  
Anna T Palatucci ◽  
Valentina Rubino ◽  
Flavia Carriero ◽  
...  
Keyword(s):  
Single Cell ◽  
Natural Killer ◽  
Label Free ◽  
Therapeutic Treatment ◽  
Non Invasive ◽  
Microfluidic Flow

Natural Killer (NK) are indicated as favorite candidates for innovative therapeutic treatment and are divided in two subclasses: immature regulatory NK CD56bright and mature cytotoxic NK CD56dim. Therefore, the ability...

Sign in / Sign up

Export Citation Format

Share Document