Non-invasive label-free investigation and typing of head and neck cancers by multimodal nonlinear microscopy

2012 ◽  
Author(s):  
Tobias Meyer ◽  
Nadine Vogler ◽  
Benjamin Dietzek ◽  
Denis Akimov ◽  
Johanna Inhestern ◽  
...  
Keyword(s):  
Head And Neck ◽  
Head And Neck Cancers ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Non Invasive

scholarly journals DNA Methylation at the Novel CpG Sites in the Promoter of MED15/PCQAP Gene as a Biomarker for Head and Neck Cancers

2014 ◽  
Vol 9 ◽  
pp. BMI.S16199 ◽  
Author(s):  
Dmitry A. Ovchinnikov ◽  
Yunxia Wan ◽  
William B. Coman ◽  
Pratibala Pandit ◽  
Justin J. Cooper-White ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Head And Neck ◽  
Transcription Initiation ◽  
Normal Tissue ◽  
Cpg Methylation ◽  
Head And Neck Cancers ◽  
Diagnostic Methods ◽  
Regulation Of Transcription ◽  
Healthy Controls ◽  
Non Invasive

Head and neck cancers (HNCs) represent a significant and ever-growing burden to the modern society, mainly due to the lack of early diagnostic methods. A significant number of HNCs is often associated with drinking, smoking, chewing beetle nut, and human papilloma virus (HPV) infections. We have analyzed DNA methylation patterns in tumor and normal tissue samples collected from head and neck squamous cell carcinoma (HNSCC) patients who were smokers. We have identified novel methylation sites in the promoter of the mediator complex subunit 15 ( MED15/PCQAP) gene (encoing a co-factor important for regulation of transcription initiation for promoters of many genes), hyper methylated specifically in tumor cells. Two clusters of CpG dinucleotides methylated in tumors, but not in normal tissue from the same patients, were identified. These CpG methylation events in saliva samples were further validated in a separate cohort of HNSCC patients (who developed cancer due to smoking or HPV infections) and healthy controls using methylation-specific PCR (MSP). We used saliva as a biological medium because of its non-invasive nature, close proximity to the tumors, easiness and it is an economically viable option for large-scale screening studies. The methylation levels for the two identified CpG clusters were significantly different between the saliva samples collected from healthy controls and HNSCC individuals (Welch's t-test returning P < 0.05 and Mann–Whitney test P < 0.01 for both). The developed MSP assays also provided a good discriminative ability with AUC values of 0.70 ( P < 0.01) and 0.63 ( P < 0.05). The identified novel CpG methylation sites may serve as potential non-invasive biomarkers for detecting HNSCC.

Organ sparing neck dissection for the treatment of head and neck cancers in children

2017 ◽  
Vol 16 (4) ◽  
pp. 56-61
Author(s):  
N.S. Grachev ◽  
◽  
I.N. Vorozhtsov ◽  
N.V. Babaskina ◽  
E.Yu. Iaremenko ◽  
...  
Keyword(s):  
Head And Neck ◽  
Neck Dissection ◽  
Head And Neck Cancers ◽  
Organ Sparing

In silico modeling of cellular probabilistic nanoparticle radiosensitization in head and neck cancers

Nanomedicine ◽  
2020 ◽  
Vol 15 (29) ◽  
pp. 2837-2850
Author(s):  
Myxuan Huynh ◽  
Ivan Kempson ◽  
Eva Bezak ◽  
Wendy Phillips
Keyword(s):  
Head And Neck ◽  
Patient Outcomes ◽  
Head And Neck Cancers ◽  
New Approach ◽  
Treatment Regimens ◽  
Therapeutic Benefits ◽  
In Silico Modeling ◽  
The Individual ◽  
Dose Deposition ◽  
Therapy Treatment

Background: The use of gold nanoparticles (AuNPs) as radiosensitizers may offer a new approach in the treatment of head and neck cancers; minimizing treatment-associated toxicities and improving patient outcomes. AuNPs promote localized dose deposition; permitting improved local control and/or dose reduction. Aim: This work aimed to address the theoretical optimization of radiation doses, fractionation and nanoparticle injection schedules to maximize therapeutic benefits. Materials & methods: Probabilistic nanoparticle sensitization factors were incorporated into the individual cell-based HYP-RT computer model of tumor growth and radiotherapy. Results: Total dose outcomes across all radiation therapy treatment regimens were found to be significantly reduced with the presence of AuNPs, with bi-weekly injections showing the most decrease. Conclusion: Outcomes suggest the need for regular AuNP administration to permit effective radiosensitization.

Postoperative chemoradiation for head and neck cancers

2006 ◽  
Author(s):  
Kumar Prabhash ◽  
V R Pai ◽  
Purvish M Parikh ◽  
Ameesh Vora
Keyword(s):  
Head And Neck ◽  
Head And Neck Cancers ◽  
Postoperative Chemoradiation
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